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Genetic Distances and Variations of Three Clupeid Species Determined by PCR Technique

  • Choi, Sang-Hoon;Yoon, Jong-Man
    • Development and Reproduction
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    • v.18 no.4
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    • pp.287-292
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    • 2014
  • In this study, seven oligonucleotides primers were shown to generate the shared loci, specific loci, unique shared loci to each species and shared loci by the three species which could be obviously calculated. Euclidean genetic distances within- and between-species were also calculated by complete linkage method with the sustenance of the hierarchical dendrogram program Systat version 13. The genomic DNA isolated from herring (Clupea pallasii), Korean anchovy (Coilia nasus) and large-eyed herring (Harengula zunashi), respectively, in the Yellow Sea, were amplified several times by PCR reaction. The hierarchical dendrogram shows three chief branches: cluster 1 (PALLASII 01, 02, 03, 04, 06 and 07), cluster 2 (NASUS 08, 09, 10, 11, 12, 13 and 14), and cluster 3 (ZUNASHI 15, 16, 17, 18, 19, 20, 21 and PALLASII 05). In three clupeid species, the shortest genetic distance displaying significant molecular difference was between individual PALLASII no. 03 and PALLASII no. 02 (0.018). Individual no. 06 of PALLASII was most distantly related to NASUS no. 11 (genetic distance = 0.318). Individuals from herring (C. pallasii) species (0.920) exhibited higher bandsharing values than did individuals from Korean anchovy (C. nasus) species (0.872) (P<0.05). As a result, this PCR analysis generated on the genetic data displayed that the herring (C. pallasii) species was widely separated from Korean anchovy (C. nasus) species. Reversely, individuals of Korean anchovy (C. nasus) species were a little closely related to those of large-eyed herring (H. zunashi) species.

Seasonal Variation in Species Composition of Fishes Collected by a Three Sided Fyke Net from Sannam Reservoir in Changwon-si, Gyeongsangnam-do (경상남도 창원시 산남저수지에서 삼각망으로 채집된 어류의 계절별 종조성)

  • Kim, Kyung Ryul;Park, Jong Hyeok;Park, Jong Sick;Choi, Mun Seong;Lee, Dae Hyun;Baeck, Gun Wook
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.52 no.6
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    • pp.745-751
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    • 2019
  • Seasonal variation in species composition of fishes in the Sannam reservoir in Changwon, Korea was determined using samples collected by a three-sided fyke net in 2018. During the study period, we collected 2,181 individuals of 9 species, with a total weight of 111,552.2 g. The dominant species were Lepomis macrochirus, Carassius carassius, Erythroculter erythropterus, and Cyprinus carpio. These species accounted for 96.1% of the total number of individuals. The number of fish species, number of individuals, biomass and species diversity indices fluctuated with the seasons. The peak number of fish species occurred in May and August, the biomass of fishes was highest in May. Whereas the number of individuals was highest in February.

Chittagong University Campus: Rich in Forest Growing Stock of Valuable Timber Tree Species in Bangladesh

  • Akter, Salena;Rahman, Md. Siddiqur;Al-Amin, M.
    • Journal of Forest and Environmental Science
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    • v.29 no.2
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    • pp.157-164
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    • 2013
  • The campus of Chittagong University in Bangladesh is rich in forest ecosystem. The campus has large area with vast tract of land planted with valuable timber tree species. The present study identifies and discovers the potential growing stock of the plantations in the campus area. This Growing stock was measured in three parameters viz. volume, biomass and organic carbon stock. Study identified thirty three economically valuable forest tree species in the plantations of Chittagong University. Out of three growing stock parameters, volume of timber was found to be low in indigenous tree species in the plantation sites other than exotic species. This might be due to their slow growth rate and low density in the plantation sites. However, biomass and organic carbon stock of trees per hactre area showed that indigenous species gather and sequester more timber and carbon respectively than introduced species. Plantations of Chittagong University campus can acquire $25.51m^3/ha$ volume of economically important tree species, where biomass and organic carbon stock is 222.33 tonne/ha and 107.48 tonne/ha respectively. This result shows a positive impression on the plantation site to be considered as good forest reserve.

New records of five species of Lepidoptera (Cosmopterigidae, Tortricidae, Pyralidae and Erebidae) from sand-dunes along the western coastline of Korea

  • Sohn, Jae-Cheon
    • Journal of Species Research
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    • v.11 no.1
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    • pp.61-66
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    • 2022
  • Three localities of coastal sand-dunes along the western coastline of Korea: Sindu-ri, Sinhap-ri, and Jaeun-do, were surveyed for the lepidopteran fauna. This survey resulted in the records of five species new to Korea: a species of Cosmopterigidae, Cosmopterix flavidella Kuroko, 2011, based on eight specimens from Sinhap-ri and Jaeun-do; a species of Tortricidae, Noduliferola abstrusa Kuznetzov, 1973, based on five specimens from Sinhap-ri and Jaeun-do; a species of Pyralidae, Maliarpha borealis Sasaki, 2012, based on four specimens from Sindu-ri; and two species of Erebidae, Ectoblemma rosella Sugi, 1982 and Metachrostis miasma (Hampson, 1891), based on one specimen for each from Jaeun-do. Three genera, Noduliferola Kuznetzov, 1973, Maliarpha Ragonot, 1888 and Ectoblemma Sugi, 1982, are introduced for the first time to the Korean fauna. Two species, Noduliferola abstrusa and Maliarpha borealis, are suggested as the indicator species for coastal sand-dunes. The superficial and genital features of the five species found from this study are described and illustrated. A photograph of the female genitalia of Ectoblemma rosella is provided for the first time.

Development of a Species Identification Method for the Egg and Fry of the Three Korean Bitterling Fishes (Pisces: Acheilognathinae) using RFLP (Restriction Fragment Length Polymorphism) Markers (제한절편 길이 다형성(RFLP) 분자마커를 이용한 납자루아과 담수어류 3종의 난과 치어 종 동정 기법 개발)

  • Choi, Hee-kyu;Lee, Hyuk Je
    • Korean Journal of Environmental Biology
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    • v.36 no.3
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    • pp.352-358
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    • 2018
  • This study aimed to develop a species identification method for the egg and fry of the three Korean bitterling fishes (Pisces: Acheilognathinae), including Acheilognathus signifer, Acheilognathus yamatsutae and Rhodeus uyekii based on the PCR-based Restriction Fragment Length Polymorphism (RFLP) markers. We conducted a field survey on the Deokchicheon River from the North Han River basin, where the three Acheilognathinae species co-occur, and also analyzed the existing sequence dataset available from the GenBank. We found coexistence of the three species at the study site. The egg and fry were obtained from the host mussels (Unio douglasiae sinuolatus) by hand from May to June 2015 and in May 2017. To develop PCR-based RFLP markers for species identification of the three Acheilognathinae fish species, restriction enzymes pinpointing species-specific single nucleotide variation (SNV) sites in mitochondrial DNA COI (cytochrome oxidase I) and cyt b (cytochrome b) genes were determined. Genomic DNA was extracted from the egg and fry and RFLP experiments were carried out using restriction enzymes Apal I, Stu I and EcoR V for A. signifer, A. yamatsutae and R. uyekii, respectively. Consequently, unambiguous discrimination of the three species was possible, as could be seen in DNA band patterns from gel electrophoresis. Our developed PCR-based RFLP markers will be useful for the determination of the three species for the young and would assist in studying the spawning patterns and reproductive ecology of Acheilognathinae fishes. Furthermore, we believe the obtained information will be of importance for future maintenance, management and conservation of these natural and endangered species.

Community Structure, Species Composition and Population Status of NTFPs of Ziro Valley in Arunachal Pradesh, India

  • Bamin, Yakang;Gajurel, Padma Raj;Paul, Ashish
    • Journal of Forest and Environmental Science
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    • v.33 no.3
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    • pp.202-225
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    • 2017
  • Non Timber Forest Products (NTFPs) has gained a lot of significance over the years as a means of income generation. Forests are playing a vital role in the supply of these products, however, due to their continuous extraction, the population of many species might have depleted. Very little information is known about community structure and population status of NTFPs. No specific studies have been made to find out the occurrence, availability of species and population status in the forests, supplying the resources. The present study has been carried out in community forests of the naturally occurring NTFPs in the temperate forest of the Ziro valley of Arunachal Pradesh. The main aim is to determine community structure, species composition and population status of NTFPs. Three forest stands viz., Nyilii, Dura and Gyachi were selected which are used by the Apatani tribe for extraction of the NTFPs. For evaluation of species composition and community characteristics, the sampling of the vegetation was done using the quadrat method. A total 137 species representing 68 families and 116 genera were recorded. Herbs represent the maximum diversity with 71 species followed by 35 shrub species and 31 tree species. The families Asteraceae and Rosaceaeae exhibited maximum representation followed by Urticaceae. The species under Fagaceae, Lauraceae, Rosaceae and Rutaceae were found to be important NTFP yielding species. Highest species richness was recorded in Nyilii having 124 species, while lowest in Dura with 102 species. Density of tree, shrub and herb ranged between 376 to $456\;individuals\;ha^{-1}$, 2848 to $3696\;individuals\;ha^{-1}$ and 31.44 to $36.64\;individuals\;m^{-2}$, respectively. The total basal area was found to be highest ($51.64m^2\;ha^{-1}$) in Dura followed by Nyilii ($25.32m^2\;ha^{-1}$) and lowest in Gyachi ($22.82m^2\;ha^{-1}$). In all the three study stands the species diversity indices showed the trend, herbs > shrubs > trees while the evenness index showed the trend as shrubs > herbs > trees. The overall species similarity index was highest (82.35%) between Dura and Gyachi. About 80% of the total recorded species showed clumped distribution while, no regular distribution was shown by any species. The three selected stands harbor about 50 important NTFP yielding species which are being used commonly by the Apatani people in their day to day life. Among the three study sites, overall diversity of NTFP was found highest in the Nyilii stand while the density of population was found better in Dura and Gyachi stands. The population of many species was found to be low due to continue harvesting without any sustainable management by the communities. All the selected forest stands have the potentiality to grow the high value NTFP yielding species and if managed properly, they can support the livelihood and economy of the local communities.

Aggressiveness of Three Snow Mold Fungi on Creeping Bentgrass Cultivars under Controlled Environment Conditions

  • Chang, Seog-Won;Jung, Geun-Hwa
    • The Plant Pathology Journal
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    • v.25 no.1
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    • pp.6-12
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    • 2009
  • Snow molds are the most important winter diseases of turfgrass in the United States and Canada. Eight isolates of three snow mold fungal species (three isolates of Typhula ishikariensis, three of T. incarnata, and two of Microdochium nivale) were collected from infected turfgrasses on golf courses. The isolates were evaluated for their relative aggressiveness on three cultivars (L-93, Penncross, and Providence) of creeping bentgrass (Agrostis palustris) under the same controlled conditions. Four plant ages (15, 19, 23 and 27 week-old plants from germination to inoculation) were evaluated for their susceptibility to the three pathogens and for the recovery of the plants. Regardless of age or cultivar of the host plant, M. nivale was found to be more aggressive and faster to infect and colonize than Typhula species. After three weeks recovery, M. nivale-inoculated plants showed higher disease severity than plants inoculated with the two Typhula species. Plants infected by Typhula species displayed no significant difference in disease severity. As creeping bentgrass plants get older, the severity of disease caused by three snow molds gradually decreases. This effect was observed in all cultivars tested, suggesting expression of age-related resistance as the bentgrass plants matured.

Estimating Detection Probabilities and Site Occupancy Rates of Three Anuran Species Using Call Surveys in Haenam Gun, Korea

  • Sung, Ha-Cheol;Kim, Su-Kyung;Cheong, Seok-Wan;Park, Shi-Ryong;Roh, Dong-Chan;Baek, Kyung-Whan;Lee, Jung-Hyun;Park, Dae-Sik
    • Journal of Ecology and Environment
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    • v.29 no.4
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    • pp.331-335
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    • 2006
  • We investigated the distribution of three anuran species, Three-striped pond frogs (Rana nigromaculata), Bullfrogs (Rana catesbeiana), and Narrow-mouthed toads (Kaloula borealis), in an administrative district, Haenam Gun, Junnam Province, Korea using volunteer call surveys. Twenty-eight volunteer call surveyors were assigned to each $2{\times}2km^2$ survey plot. Call surveys on whether the species are present or not were conducted for 5 minutes between 30 minutes after sunset and the midnight on rice fields and ponds from 10 April to 28 August in 2005. Depending on species, call surveys were carried out at seven to 28 plots with average 8.4 to 10.7 visits per the plot. We calculated the detection probabilities and occupancy rates of the three species using four models with three covariates: temperature, humidity, and the amount of water at the habitat. The model average detection probabilities of three anuran species of R. nigromaculata, R. catesbeiana, and K. borealis were 0.53, 0.74, and 0.41 respectively, and the site occupancy rates of them were 0.93, 0.94, and 0.86 respectively. Our results indicate that R. nigromaculata, R. catesbeiana, and K. borealis are common in Haenam Gun.

Morphological Study of the Marine Algal Genus Padina (Dictyotales, Phaeophyceae) from Southern Philippines: 3 Species New to Philippines

  • Geraldino, Paul John L.;Liao, Lawrence M.;Boo, Sung-Min
    • ALGAE
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    • v.20 no.2
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    • pp.99-112
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    • 2005
  • This monographic study presents morphological descriptions of eight species of Padina collected from the Visayas and Mindanao regions of southern Philippines, including distributions of each species and a taxonomic key for all the species examined. Of these species, three are new records for the Philippines, namely: P. fernandeziana Skottsberg and Levring, P. jonesii Tsuda and P. moffittiana Abbott and Huisman. One species, P. antillarum (Kützing) Piccone, represents a new nomenclatural record, which is applied to a Philippine species for the first time. Four species previously reported in the Philippines are reconfirmed and described P. australis Hauck, P. minor Yamada, P. boryana Thivy and P. sanctae-crucis Borgesen. All eight species studied have distromatic thalli, except for P. antillarum which is tetrastromatic. Three of these have indusiate sporangia, namely: P. sanctae-crucis, P. moffittianaand P. fernandeziana.