• Genes and Genomics
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• v.40 no.12
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• pp.1319-1329
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• 2018

SSRs were successfully isolated from the Perilla crop in our current study, and used to analyze Perilla accessions from East Asia. Analyses of the clear genetic diversity and relationship for Perilla crop still remain insufficient. In this study, 40 new simple sequence repeat (SSR) primer sets were developed from RNA sequences using transcriptome analysis. These new SSR markers were applied to analyze the diversity, relationships, and population structure among 35 accessions of the two cultivated types of Perilla crop and their weedy types. A total of 220 alleles were identified at all loci, with an average of 5.5 alleles per locus and a range between 2 and 10 alleles per locus. The MAF (major allele frequency) per locus varied from 0.229 to 0.943, with an average of 0.466. The average polymorphic information content (PIC) value was 0.603, ranging from 0.102 to 0.837. The genetic diversity (GD) ranged from 0.108 to 0.854, with an average of 0.654. Based on population structure analysis, all accessions were divided into three groups: Group I, Group II and the admixed group. This study demonstrated the utility of new SSR analysis for the study of genetic diversity and population structure among 35 Perilla accessions. The GD of each locus for accessions of cultivated var. frutescens, weedy var. frutescens, cultivated var. crispa, and weedy var. crispa were 0.415, 0.606, 0.308, and 0.480, respectively. Both weedy accessions exhibited higher GD and PIC values than their cultivated types in East Asia. The new SSR primers of Perilla species reported in this study may provide potential genetic markers for population genetics to enhance our understanding of the genetic diversity, genetic relationship and population structure of the cultivated and weedy types of P. frutescens in East Asia. In addition, new Perilla SSR primers developed from RNA-seq can be used in the future for cultivar identification, conservation of Perilla germplasm resources, genome mapping and tagging of important genes/QTLs for Perilla breeding programs.

• Journal of The Korean Society of Grassland and Forage Science
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• v.39 no.1
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• pp.31-38
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• 2019

This study was carried out to investigate the growth characteristics, yield, and chemical compositions of winter forage crops cultivated after rice harvest in the central inland region. The experimental design was arranged in a randomized block design with three replications. The treatments consisted of 4 species (Rye, Oat, Triticale and Italian ryegrass), and varieties were "Marton", "Donghan", "Shinyoung" and "Kowinnearly", respectively. As a result, dry matter yield and TDN yield were higher in rye than in the other winter forage crops, and lowest in Oat(p<0.05). Crude protein and crude fat content were significantly higher in Italian ryegrass(p<0.05). However, Crude ash and ADF content did not show significant difference among winter forage crops. NDF content was higher in order of Rye > Triticale > Oat > Italian ryegrass(p<0.05). TDN and total amino acid content (EAA+NEAA) were higher in order of winter forage crop with high crude protein content (Italian ryegrass > Oat > Triticale > Rye). Total mineral content was higher in order of Rye > Triticale > Oat > Italian ryegrass(p<0.05), and total free sugar content was higher in order of Oat > Italian Ryegrass > Triticale > Rye(p<0.05). Compared with the results above, Italian ryegrass and Oat are high in crude protein, TDN, amino acid and free sugar content. Rye and Triticale have the merit that feed value is decreased but high yield(dry matter and TDN yield) can be maintained. Therefore, it is advantageous to grow Rye and Triticale as winter forage crops after rice harvest in the central inland region.

• Korean journal of applied entomology
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• v.58 no.3
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• pp.219-224
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• 2019

The super mealworm, Zophobas morio, is mainly used as food for pets, fish, reptiles, amphibians, birds, and as snacks. An experiment was conducted to investigate the effects of temperature, supplementary feeding, and spawning interval on super mealworm rearing. To efficiently rear this species, the insects were bred at a temperature of $27^{\circ}C$ or higher and reached a weight of over 0.6 g at 80 days after hatching. Supplementary feed (with high protein content: 10% of soybean meal and 10% of fish meal) enabled larvae to reach a weight of over 0.7 g at 80 days after hatching. Mass rearing of super mealworm requires increase in egg production (i.e., high yield) and uniformity of larvae. Adults were transferred to spawning bases every 5, 10, or 15 days (three treatments), which resulted in a total of 7,256, 5,439, 2,060 hatched larvae, respectively. It is possible to obtain more than 7,000 larvae by transferring the egg-laying frame to the spawning base at intervals of 5 days and with nine spawning operations; this procedure generates larvae weighing over 0.68 g each.

• The Plant Pathology Journal
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• v.35 no.4
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• pp.381-388
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• 2019

For several years, temperatures in the Korean peninsula have gradually increased due to climate change, resulting in a changing environment for growth of crops and vegetables. An associated consequence is that emerging species of insect vector have caused increased viral transmission. In Jeju Island, Korea, occurrences of viral disease have increased. Here, we report characterization of five newly collected turnip mosaic virus (TuMV) isolates named KBJ1, KBJ2, KBJ3, KBJ4 and KBJ5 from a survey on Jeju Island in 2017. Full-length cDNAs of each isolate were cloned into the pJY vector downstream of cauliflower mosaic virus 35S and bacteriophage T7 RNA polymerase promoters. Their fulllength sequences share 98.9-99.9% nucleotide sequence identity and were most closely related to previously reported Korean TuMV isolates. All isolates belonged to the BR group and infected both Chinese cabbage and radish. Four isolates induced very mild symptoms in Nicotiana benthamiana but KBJ5 induced a hypersensitive response. Symptom differences may result from three amino acid differences uniquely present in KBJ5; Gly(382)Asp, Ile(891)Val, and Lys(2522)Glu in P1, P3, and NIb, respectively.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.32-32
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• 2019

Mutation breeding is the useful tool to improve agronomic traits in various crop species. Soybean is most important crop and is rich in protein and oil contents. Despite of the importance as economic value and various genetic resource of soybean, there have been limited studies of genetic relationship among mutant resources through radiation breeding. In this study, the agronomical phenotype for selecting various genetic resources was evaluated in 528 soybean mutant lines. As a result, 210 soybean mutants with their original cultivars were selected with various traits. We named 210 selected lines as Mutant Diversity Pool (MDP). The genetic diversity and the relationship of the MDP were investigated using TRAP and TE-TRAP markers. In TRAP analysis, sixteen primer combination (PC)s were used and a total of 551 fragments were amplified. The highest (84.00%) and the lowest (32.35%) polymorphism levels were showed in PC MIR157B+Ga5 and B14G14B+Ga3, respectively. The mean of PIC values was 0.15 ranging from 0.07 in B14G14B+Sa12 to 0.23 in MIR157B+Sa4. Phylogenetic and population structure analysis indicated that the 210 MDP lines dispersed to four groups among the wild types and their mutants. The highest genetic diversity among populations was observed between lines Paldal and 523-7 (Fst=0.409), whereas the lowest genetic diversity was between population KAS360-22 and 94seori (Fst=0.065). AMOVA showed 11.583 (21.0%) and 43.532 (79.0%) variations in inter and intra mutant population, respectively. Overall, the genetic similarity of each intra mutant populations was closer than that of inter mutant population. A total of 408 fragments were amplified in the 210 MDP using twelve PCs of TE-TRAP markers that were obtained from a combination of three TIR sequence of transposable elements (MITE-stowaway; M-s, MITE-tourist; M-t, PONG). The highest (77.42%) and the lowest (56.00%) polymorphism levels were showed in PONG+Sa4 and PONG+Sa12, respectively. The mean of PIC values was 0.15 ranging from 0.09 in M-s+Sa4 and M-s+Ga5 to 0.21 in M-t+Ga5. AMOVA of M-s showed 2.209 (20%) and 8.957 (80%) variations in inter and intra mutant population, respectively. AMOVA of M-t showed 2.766 (18%) and 12.385 (82%) variations in inter and intra mutant population, respectively. AMOVA of PONG showed 3.151 (29%) and 7.646 (71%) variations in inter and intra mutant population, respectively. According to our study, the PONG had higher inter mutant population and lower intra mutant population. This mean was that for aspect of radiation sensitivity, M-s and M-t showed higher mobility than that of PONG. Our results suggest that the TRAP and the TE-TRAP markers may be useful for assessing the genetic diversity and relationship among soybean MDP and help to improve our knowledge of soybean mutation/radiation breeding.

• Journal of Korean Society of Forest Science
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• v.107 no.4
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• pp.402-411
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• 2018

This study was conducted to develop ingrowth estimation equations on Pinus densiflora found in Gangwon Province and in the center of Korean Peninsula, based on the National Forest Inventory (NFI)'s permanent sampling plot data. For this study, identical sampling plots in $5^{th}$ and $6^{th}$ NFI data were collected in order to identify ingrowth amounts for the last 5 years. Following two-stage approaches in developing the ingrowth estimation equations, the logistic regression model was used in the first stage to estimate the ingrowth probability. In the second stage, regression analysis on sampling plots with ingrowth occurrence was used to estimate the ingrowth amount. A candidate model was finally selected as an optimal model after a verification based on three evaluation statistics which include mean difference (MD), standard deviation of difference (SDD) and standard error of difference (SED). In results, a logistic regression model based on the number of sampling plot which did not result in ingrowth (model VI), was selected for an ingrowth probability estimation equation and exponential function including the species composition (SC) variable was optimal for an ingrowth estimation equation (model VII). The ingrowth estimation equations developed in this study also evaluated the estimation ability in various forest stand conditions, and no particular issue in fitness or applicability was observed.

• Korean Journal of Heritage: History & Science
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• v.44 no.3
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• pp.4-17
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• 2011

The Baekje's cotton fabrics were excavated from 'Neungsan-ri temple site in Buyeo' (September 1999-April 2000) and stored at Buyeo National Museum after conservation treatment. In this study, it carred out non-destructive, chemical and morphological analysis for fiber identification, also considered on influx, features and difference between the 'Baek-cheop-po(Three kingdom period's cotton fiber)' and 'Mok-myeon(imported by Munikjeom, late Korea dynasty)'. As a result, the fiber proved cellulose fiber through analytical researches like color reaction, FT-IR(chemical analysis). It was also confirmed lumens, typical dimensional structure(morphological analysis) as an features of cotton fiber. The fiber was the first evidence in ancient Korea's cotton. But it can not prove that whether weaving were made in Baekje's area. However there were documentation that people in Beakje make cloth to silk fabric from 'Mahan period'. We can suppose that they have had an old weaving techniques. This study has a great historical, academic values as the only evidence for the hypothesis of a weaving technique of the Baekje's cotton. Through comparison to each region's ancient cottons, we can investigate the species of Baekje's cotton and ancient Korea cotton's influx.

• Korean Journal of Environmental Biology
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• v.37 no.1
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• pp.72-81
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• 2019

Bisphenol A (BPA), a representative endocrine disrupting chemicals, has adverse effects on growth, development and reproduction in aquatic organisms. The object of this study was to investigate the modulation of antioxidant enzyme-coding genes using quantitative real time RT-PCR (qRT-PCR), enzyme activity and total protein content, to understand oxidative stress responses after exposure to BPA for 48 h in brackish water flea Diaphanosoma celebensis. The BPA ($3mg\;L^{-1}$) significantly upregulated the expression of Cu/Zn-SOD, Mn-SOD, and catalase (CAT) mRNA. Three GST isoforms (GST-kappa, GST-mu, and GST-theta) mRNA levels significantly increased at the rate of $0.12mg\;L^{-1}$ of BPA. In particular, GST-mu showed the highest expression level, indicating its key role in antioxidant response to BPA. SOD activity was induced with a concentration-dependent manner, and total protein contents was reduced. These findings indicate that BPA can induce oxidative stress in this species, and these antioxidants may be involved in cellular protection against BPA exposure. This study will provide a better understanding of molecular mode of action of BPA toxicity in aquatic organisms.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.22 no.3
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• pp.55-71
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• 2019

The purpose of this study is to propose an evaluation method to assess green environments of streetscapes to improve urban street green spaces in Jeonju City. Through a rapid assessment of urban street green spaces, we suggest an objective basis for expanding street green space as well as for adopting sustainable maintenance and improvement measures. We choose 12 sections of streetscapes (roads and sidewalks) to investigate existing street conditions which have more than four lanes and function as major road axes. Six large roads and six medium roads of Jeonju City center area are investigated as pilot assessment study sites. Site inventory checklists consist of environmental characteristics of streetscape, street tree status, and planting condition evaluation. Environmental characteristics of streetscapes are composed of physical and neighborhood factors. For instance, items for physical factors are types and width of road/sidewalks, paving materials, tree protection materials, and green strip. And surrounding landuse is a neighborhood factor. Assessment items for street tree status are street plant names (tree/shrubs/ground cover), size, and planting intervals. Planting condition evaluation items are tree shape, damage, canopy density, and planting types with existence of adjacent green space. Evaluation results are classified into three levels such as A(maintain or repair), B(greening enhancement), and C(structural improvement). In case of grade A, streetscapes have enough sidewalk width for maintaining green strip and a multi-layered planting(in large road only) with fairly good growing conditions of street trees. For grade B and C, streetscapes have a moderate level of sidewalk width with a single street tree planting. In addition, street tree growing conditions are appeared poor so that green enhancement or maintenance measures are needed. For median, only grade B and C are found as its planting growing foundations are very limited in space. As a result, acquiring enough sidewalk space is essential to enhance ecological quality of urban street green. Especially, it is necessary to have green strip with reasonable widths for plant growing conditions in sidewalks. In addition, we need to consider native species with multi-layer plant compositions while designing street green.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.22 no.3
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• pp.15-30
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• 2019

Changpo Lake was created as a part of a land reclamation for refugee self-helping projects. It shows characteristics of a fresh water lake, and still retains the early appearance of reclamation that surrounding regions have not been developed into farm lands. Shallow wetland has formed around the lake, which provides great conditions for diverse lives, and surrounding earthiness is favorable for growth of vegetation and restoration of the ecosystem. However, as facilities of the Muan International Airport nearby Changpo Lake are expanding and barns are being constructed, artificialness is gradually increasing. Particularly, since pollution sources such as sport facilities, farm lands and barns are scattered around Changpo Lake, pollutants are flowing in constantly. Accordingly, the results for setting up management areas according to the spatial characteristics and creating natural ecological spaces near Changpo Lake, Taebongcheon stream and Hakgyecheon stream are as follows. First, the creation of a natural eco-friendly waterfront space should be promoted by securing the health of the aquatic ecosystem and restoring species and the ecosystem. In addition, a consultative body needs to be formed to lead local residents to participating in river investigation and monitoring, maintenance, and management through role sharing. Second, the basic direction of the spatial management plan is to keep the unique charm of Changpo Lake, maintain harmony with nature, create diverse waterfront areas, and secure the continuity of Changpo Lake and inflow streams. Moreover, the area should be divided into three zones such as a conservation zone, a restoration zone and a waterfront zone, and for each zone, the preservation of vegetation, the creation of ecological wetlands and restoration of the ecotone and ecological nature need to be promoted. Third, facilities and activity programs for each space of Changpo Lake should be operated for efficient management of protected areas. In order to suit the status of each space, biological habitats, water purification spaces, experiential and learning spaces, and convenience and rest spaces should be organized and designated as research, monitoring, education, and tourism areas. Accordingly, points of interest should be set up within the corresponding area. In this study, there are many parts that need to be supplemented for immediate implementation since the detailed plans and project costs for the promotion of programs by area are not calculated. Therefore, it is necessary to make detailed project plans and consider related projects such as water quality, restoration of habitats, nature learning and observation, and experience of ecological environments based on the categories such as research, monitoring, education and tourism in the future.