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http://dx.doi.org/10.5423/PPJ.NT.11.2018.0238

Five Newly Collected Turnip Mosaic Virus (TuMV) Isolates from Jeju Island, Korea are Closely Related to Previously Reported Korean TuMV Isolates but Show Distinctive Symptom Development  

Hu, Wen-Xing (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Kim, Byoung-Jo (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Kwak, Younghwan (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Seo, Eun-Young (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Kim, Jung-Kyu (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Han, Jae-Yeong (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Kim, Ik-Hyun (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Lim, Yong Pyo (Molecular Genetics and Genomics Laboratory, Department of Horticulture, College of Agriculture and Life Sciences, Chungnam National University)
Cho, In-Sook (National Institute of Horticultural & Herbal Science, Rural Development Administration)
Domier, Leslie L (Soybean/Maize Germplasm, Pathology, and Genetics Research Unit, United States Department of Agriculture-Agricultural Research Service)
Hammond, John (Floral and Nursery Plants Research Unit, United States National Arboretum, United States Department of Agriculture-Agricultural Research Service)
Lim, Hyoun-Sub (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Publication Information
The Plant Pathology Journal / v.35, no.4, 2019 , pp. 381-388 More about this Journal
Abstract
For several years, temperatures in the Korean peninsula have gradually increased due to climate change, resulting in a changing environment for growth of crops and vegetables. An associated consequence is that emerging species of insect vector have caused increased viral transmission. In Jeju Island, Korea, occurrences of viral disease have increased. Here, we report characterization of five newly collected turnip mosaic virus (TuMV) isolates named KBJ1, KBJ2, KBJ3, KBJ4 and KBJ5 from a survey on Jeju Island in 2017. Full-length cDNAs of each isolate were cloned into the pJY vector downstream of cauliflower mosaic virus 35S and bacteriophage T7 RNA polymerase promoters. Their fulllength sequences share 98.9-99.9% nucleotide sequence identity and were most closely related to previously reported Korean TuMV isolates. All isolates belonged to the BR group and infected both Chinese cabbage and radish. Four isolates induced very mild symptoms in Nicotiana benthamiana but KBJ5 induced a hypersensitive response. Symptom differences may result from three amino acid differences uniquely present in KBJ5; Gly(382)Asp, Ile(891)Val, and Lys(2522)Glu in P1, P3, and NIb, respectively.
Keywords
hypersensitive response; mild symptoms; turnip mosaic virus;
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