• Title/Summary/Keyword: thermo-stability

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Enhanced Stability of Tyrosine Phenol-Lyase from Symbiobacterium toebii by DNA Shuffling

  • Kim, Jin-Ho;Song, Jae-Jun;Kim, Bong-Gyun;Sung, Moon-Hee;Lee, Sang-Chul
    • Journal of Microbiology and Biotechnology
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    • v.14 no.1
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    • pp.153-157
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    • 2004
  • Tyrosine phenol-lyase (TPL) is a useful enzyme for the synthesis of pharmaceutical aromatic amino acids. In the current study, sequential DNA shuffling and screening were used to enhance the stability of TPL. Twenty-thousand mutants were screened, and several improved variants were isolated. One variant named A13V, in which the $13^{th}$ amino acid alanine was substituted by valine, exhibited a higher temperature and denaturant stability than the wild-type TPL. The purified mutant TPL, A13V, retained about 60% of its activity at $76^\circ{C}$, whereas the activity of the wild-type TPL decreased to less than 20% at the same temperature. Plus, A13V exhibited about 50% activity with 3 M urea, while the wild-type TPL lost almost all its catalytic activity, indicating an increased denaturant tolerance in the mutant A13V. It is speculated that the substitution of Val for the Ala in the $\beta$-strand of the N-terminal arm was responsible for the heightened stabilization, and that the current results will contribute to further research on the structural stability of TPL.

Calculation of Combustion Stability Limits Using Linear Stability Analysis in Liquid Rocket Engines (액체 로켓엔진에서 선형 연소 불안정 해석을 이용한 연소 안정한계 곡선 계산)

  • Sohn, Chae-Hoon;Moon, Yoon-Wan;Huh, Hwan-Il
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.32 no.10
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    • pp.93-101
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    • 2004
  • A method to calculate stability limits is investigated to predict the characteristics of high-frequency combustion instability in liquid-propellant rocket engine. It is based on the theory of linear stability analysis proposed in previous works and useful to predict combustion stability at the beginning stage of engine development. The system of equations governing reactive flow in combustor has the simplified and linearized forms. The overall equation expressing stability limits is adopted. The procedures to evaluate quantitatively each term included in the equation are proposed. The thermo-chemical properties and flow variables required in the evaluation can be obtained from calculation of thermodynamic equilibrium, CFD results, and experimental test data. Based on the existent data, stability limits are calculated with actual rocket engine (KSR-III rocket engine). The present calculations show the reasonable stability limits in a quantitative manner and the stability characteristics of the engine are discussed. The prediction from linear stability analysis could be serve as the first approximation to the true prediction.

Systhesis and Characterization of energetic plasticizers, Formal (포르말계 에너지화 가소제의 합성 및 특성분석)

  • 김진석;이근득;조진래
    • Journal of the Korea Institute of Military Science and Technology
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    • v.5 no.4
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    • pp.49-56
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    • 2002
  • For the purpose of the increase in the performance and thermal stability of PBX's, the mixed formal consisting of BDNPF, DNPBF and BDNBF were synthesized. In order to find out the optimal condition for the synthesis of energetic plasticizer, BDNPF, DNPBF and BDNBF, the synthetic procedures have been investigated. We synthesized DNP-OH and DNB-OH through oxidative nitration and controlled various composition of mixed formal by $H_{2}SO_{4}$ and s-trioxane to investigate optimal composition, and then characterized its thermo-physical properties.

Azo-Dyes Photo-Aligning; Physics and Applications

  • Chigrinov, V.G.;Kwok, H.S.;Takada, Hirokazu;Takatsu, Haruyoshi
    • 한국정보디스플레이학회:학술대회논문집
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    • 2005.07a
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    • pp.759-763
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    • 2005
  • This review presents the status of our research in liquid crystal display (LCD) photo-aligning. We present the basic mechanisms of the photo-induced order in various photo-aligning materials and in azo-dye layers in particular and show that photo-aligning methods can provide a controllable pretilt angle and anchoring energy of the liquid crystal cell, as well as its high thermo and UV stability. The photo-aligning of various types of liquid crystal displays is also discussed.

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Stability and Thermo-physical Properties of Nanofluids and Its Applications (나노유체의 분산안정성 및 열물성치와 그 응용에 관한 연구)

  • Hwang, Y.;Lee, K.;Kim, K.;Lee, J.
    • 한국전산유체공학회:학술대회논문집
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    • 2008.03b
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    • pp.474-478
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    • 2008
  • It has been shown that a nanofluid consisting of nanoparticles dispersed in base fluid has much higher effective thermal conductivity than pure fluid. In this study, four kinds of nanofluids such as multiwalled carbon nanotube (MWCNT) in water, CuO in water, SiO2in water, and CuO in ethylene glycol, are produced. Their thermal conductivities are measured by a transient hot-wire method. The thermal conductivity of water-based MWCNT nanofluid is shown to be increased by up to 11.3% at a volume fraction of 0.01. The measured thermal conductivities of MWCNT nanofluids are higher than those calculated with Hamilton-Crosser's model due to neglecting solid-liquid interaction at the interface. The results show that the thermal conductivity enhancement of nanofluids depends on the thermal conductivities of both particles and the base fluid. Stability of nanofluids is estimated by UV-vis spectrum analysis. Stability of nanofluid depends on the type of base fluid and the suspended particles. Also it can be improved in addition of a surfactant.

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Stability Analysis Using the Amplitude Envelope of Dynamic Pressure in the Rocket Combustor (로켓 연소기의 동압 진폭엔벨롭을 이용한 안정성 해석)

  • Lee, Soo Yong
    • Journal of the Korean Society of Propulsion Engineers
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    • v.25 no.1
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    • pp.42-49
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    • 2021
  • As a measure of susceptibility on the combustion instability, thermo-acoustic instabilities in rocket combustion system was considered for the estimation of the operational stability margin. Growth rate, which governs the asymptotic stability behavior of the system, was determined from the dynamic data measured during combustion tests in order to understand the dynamic characteristics of combustor system. Frequency transform technique was first applied to determine the system parameters such as growth rate and/or damping coefficient for an interested mode from the time series pressure data, and the PDFs of pressure amplitude were extracted from the amplitude envelope of pressure oscillation for the stochastic analysis.

High Temperature Characteristics of Commercially Available Anion Exchange Membrane for Alkaline Water Electrolysis (알칼리 수전해를 위한 상용 음이온교환막의 고온 특성)

  • JANG, SU-YOEN;RYU, CHEOL-HWI;HWANG, GAB-JIN
    • Journal of Hydrogen and New Energy
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    • v.33 no.4
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    • pp.330-336
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    • 2022
  • In order to evaluate the possibility as a separator in alkaline water electrolysis, the high temperature characteristics were evaluated by measuring the membrane resistance and durability of 5 types of commercial anion exchange membranes in 7 M KOH solution and at 80℃. The membrane resistance of AEM membrane measured in 7 M KOH solution and at 80℃ had a lower value of about 8-24 times compared to the other membranes. The durability of AEM membrane tested with the soaking time in 7 M KOH solution and at 80℃ showed a very good stability and that of FAAM40 and FAAM75-PK showed secondly a good stability. The thermal stability with the soaking time in 7 M KOH solution and at 80℃ of FAAM40 and FAAM75-PK membrane analyzed by thermo-gravimetric analysis showed a good stability compared to the other membranes.

A New Class of Sol-Gel Transition Hydrogels for Macromolecular Delivery

  • Lee, Yu-Han;Park, Sung-Young;Chung, Hyun-Jung;Park, Tae-Gwan
    • Proceedings of the Polymer Society of Korea Conference
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    • 2006.10a
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    • pp.52-53
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    • 2006
  • A new series of PEO-PPO-PEO and PPO-PEO-PPO copolymers having several hydroxyl groups on the PPO chain segment were synthesized, further modified with various poly(lactic acid) PLA oligomeric chains to confer physical stability after thermo-gelation in the body fluid. Gel stability was endowed by either increasing hydrophobic interaction between PLA chains or inducing stereocomplex formation between enatiomeric isomers of PLA chains. Macromolecular drugs were incorporated within the gels and their release patterns were investigated using Pluronic F127 as a control.

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Thermo-Oxidative Stability of Epoxy/Carbon Fiber Composites under Thermal Cycling Conditions (반복되는 열주기환경에 노출된 에폭시/탄소섬유 복합재료의 열산화 안정성 연구)

  • 남재도
    • The Korean Journal of Rheology
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    • v.10 no.2
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    • pp.106-112
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    • 1998
  • 항공기용 소재로 개발된 에폭시/탄소섬유 복합재료가 매트릭스의 유리전이온도에 육 박하는 온도주기를 경험할 때 발생하는 복합재료의 구조 및 물성변화를 실험과 모델링을 통 하여 연구하였다. 복합재료의 표준 경화온도인 177$^{\circ}C$에서 2시간 체류시킨후 냉각시키는 바 복 열주기는 복합재료를 취약하게 하여 결국 표면에서부터 미세크랙이발생한다는 것을 알수 있었다. 이러한 열주기에 따른 미세크랙 현상은 매트릭스의 분해반응이 발생할수 있는 유효 표면적을 증가시키고 크랙을 통하여 산소의투과를 용이하게 함으로서 산화반응을 가속화하 여 복합재료시편의 무게감소를 가속화시키는 것으로 판단된다. 특히 본 연구에서는 등온과 등속도 승온조건을 주기적으로 반복하는 열주기 조건을 해석하기 위하여 열주기 조건을 특 정온도에서의 등오시간으로 전환할 수 있는 e-quivalent cycle time(ECT)를 제안하였고 이 를 이용하여 열주기에 의한 복합재료 손상의 가속/감속 현상을 규명할수있었다.

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Experimental investigation on thermal behavior, sound absorption, and flammability of natural fibre polymer composites

  • Ravi Kumar, B.;Hariharan, S.S.
    • Structural Engineering and Mechanics
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    • v.76 no.5
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    • pp.613-618
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    • 2020
  • Exhausting oil resources and increasing pollution around the world are forcing researchers to look for new, renewable, biodegradable materials to lead sustainable development. The use of fiber reinforced composites based on natural fibres has increasingly begun as prospective materials for various engineering applications in the automotive, rail, construction and aerospace industries. The natural fiber chosen to make the composite material is plant-based fibre, e.g. jute fibre, and hemp fibre. Thermosetting polymer based Epoxy (LY556) was utilized as matrix material and The composites were produced using hand lay-up technique. The fabricated composites were tested for acoustic testing, thermo-gravimetric analysis (TGA) and flammability testing to asses sound absorption, thermal decomposition and fire resistivity of the structures. Hemp fibre composites have shown improved thermal stability over Jute fibre composites. However, the fire resistance characteristics of jute fibre composites are better as compared to hemp fibre composites. The sound absorption coefficient of composites was found to enhance with the increase of frequency.