• The Korean Journal of Physiology and Pharmacology
• /
• v.1 no.6
• /
• pp.625-637
• /
• 1997

Calcium ions are implicated in a variety of physiological functions, including enzyme activity, membrane excitability, neurotransmitter release, and synaptic transmission, etc. Calcium antagonists have been known to be effective for the treatment of exertional angina and essential hypertension. Selective and nonselective voltage-dependent calcium channel blockers also have inhibitory action on the acute and tonic pain behaviors resulting from thermal stimulation, subcutaneous formalin injection and nerve injury. This study was undertaken to investigate the effects of iontophoretically applied $Ca^{++}$ and its antagonists on the responses of WDR (wide dynamic range) cells to sensory inputs. The responses of WDR cells to graded electrical stimulation of the afferent nerve and also to thermal stimulation of the receptive field were recorded before and after iontophoretical application of $Ca^{++}$, EGTA, $Mn^{++}$, verapamil, ${\omega}-conotoxin$ GVIA, ${\omega}-conotoxin$ MVIIC and ${\omega}-agatoxin$ IVA. Also studied were the effects of a few calcium antagonists on the C-fiber responses of WDR cells sensitized by subcutaneous injection of mustard oil (10%). Calcium ions and calcium channel antagonists ($Mn^{++}$, verapamil, ${\omega}-conotoxin$ GVIA & ${\omega}-agatoxin$ IVA) current-dependently suppressed the C-fiber responses of WDR cells without any significant effects on the A-fiber responses. But ${\omega}-conotoxin$ MVIIC did not have any inhibitory actions on the responses of WDR cell to A-fiber, C-fiber and thermal stimulation. Iontophoretically applied EGTA augmented the WDR cell responses to C-fiber and thermal stimulations while spinal application of EGTA for about $20{\sim}30\;min$ strongly inhibited the C-fiber responses. The augmenting and the inhibitory actions of EGTA were blocked by calcium ions. The WDR cell responses to thermal stimulation of the receptive field were reduced by iontophoretical application of $Ca^{++}$, verapamil, ${\omega}-agatoxin$ IVA, and ${\omega}-conotoxin$ GVIA but not by ${\omega}-conotoxin$ MVIIC. The responses of WDR cells to C-fiber stimulation were augmented after subcutaneous injection of mustard oil (10%, 0.15 ml) into the receptive field and these sensitized C-fiber responses were strongly suppressed by iontophoretically applied $Ca^{++}$, verapamil, ${\omega}-conotoxin$ GVIA and ${\omega}-agatoxin$ IVA. These experimental findings suggest that in the rat spinal cord, L-, N-, and P-type, but not Q-type, voltage-sensitive calcium channels are implicated in the calcium antagonist-induced inhibition of the normal and the sensitized responses of WDR cells to C-fiber and thermal stimulation, and that the suppressive effect of calcium and augmenting action of EGTA on WDR cell responses are due to changes in excitability of the cell.

• Journal of Life Science
• /
• v.26 no.12
• /
• pp.1360-1366
• /
• 2016

Heat shock proteins (HSPs) are induced in response to various physiological or environmental stressors. However, the transcriptional activation of HSPs is regulated by a family of heat shock factors (HSFs). Fish models provide an ideal system for examining the biochemical and molecular mechanisms of adaptation to various temperatures and water environments. In this study, we examined the pattern differentials of heat shock factor 1 (HSF1) and expression of heat shock protein 70 (HSP70) in response to thermal stress in goldfish and mouse hepatocyte cultures by immune-blot analysis. Goldfish HSF1 (gfHSF1) changed from a monomer to a trimer at $33^{\circ}C$ and showed slightly at $37^{\circ}C$, whereas mouse HSF1 (mHSF1) did so at $42^{\circ}C$. This experiment showed similar results to a previous study, indicating that gfHSF1 and mHSF1 play different temperature in the stress response. We also examined the activation conditions of the purified recombinant proteins in human HSF1 (hmHSF1) and gfHSF1 using CD spectroscopy and immune-blot analysis. The purified recombinant HSF1s were treated from $25^{\circ}C$ to $42^{\circ}C$. Structural changes were observed in hmHSF1 and gfHSF1 according to the heat-treatment conditions. These results revealed that both mammal HSF1 (human and mouse HSF1) and fish HSF1 exhibited temperature-dependent changes; however, their optimal activation temperatures differed.

• Fibers and Polymers
• /
• v.7 no.4
• /
• pp.442-445
• /
• 2006

The purpose of this study was to compare the subjective fabric hand evaluation and wear comfort of PET treated by Ultraviolet and to evaluate the subjective results from the investigation of microclimate temperature. The subjective hand evaluation was performed by 20 subjects (age: 20-25) with 5-point scale questionnaires to investigate the change of PET knit fabrics treated for different times, specifically, 0, 30 and 90 minutes. The questionnaires were composite with 8 questions; roughness, smoothness, wetness, stiffness, coolness, touch, preference, and comfort. In order to evaluate sensations of wear comfort, we made garments with UV treated fabric and five female students were tested. They walked at the speed of 6.7 km/ hr for 15 minutes in an environment, which was controlled at $29^{\circ}C,\;75{\pm}5%$ RH. Before and after exercising, the microclimate under clothing and subjective wear comfort was measured. The results of subjective evaluation of fabric hand were that untreated and treated for 30 minutes PET were recognized as similar and have a good evaluation on comfort, preference, and touch. According to the result of wear comfort, clothing treated by UV for 90 minutes had the lowest value on the thermal and humidity sensations. In addition, the value of tactile and comfort sensation was the highest on the clothing treated by UV for 90 minutes. In the case of objective evaluation, PET treated for 90 minutes was the lowest on microclimate humidity. PET irradiated by UV for 90 minutes was more 'cool' in thermal sensation and more 'dry' in wet sensation. Accordingly, it was consistent with the result of subjective wear comfort.

• The Korean Journal of Community Living Science
• /
• v.21 no.1
• /
• pp.117-124
• /
• 2010

Recently, research on variables associated with hair styles are increasing with the spreading of total coordinated intention in the fields of clothing and cosmetology. In the present study, we focused on examining the skin temperature on the scalp(the vertex, temporal, and occiput), humidity on the occiput, skin temperatures (the forehead, back of ear, back of neck, upper back, abdomen, forearm, hand, thigh, calf, and foot), rectal temperature, total body mass loss by hair styles in a thermal neutral environment. Four young females participated as subjects. For a certain period, each subject had the five different hair styles in a random order: (1)Straight short hair(SS), (2)Perm waved short hair(PS), (3) Straight long hair(SL), (4)Perm waved long hair(PL), (5)Ponytail style(PT). Subjects wore briefs, bra, shirts with long sleeves, long legged training pants, and socks. The environmental variables of a climatic chamber were kept constant at $21{\pm}0.5^{\circ}C$ of air temperature and 55${\pm}$5%RH of air humidity. The results indicated the following: The skin temperature and humidity on the scalp did not show any significant differences among five different hair styles, but straight long hair style(SL)was the highest on the vertex, temporal, and occiput. The temporal skin temperature($29.8\sim30.8^{\circ}C$) was the highest, while the vertex skin temperature($28.7\sim30.1^{\circ}C$) was the lowest. Humidity on the occiput was the highest in the perm waved long hair style(PL). The back of ear skin temperature was significantly lower in the ponytail style(PT) than in the other four hair styles(p<.001). In the straight long hair style(SL), the skin temperature on the back of the neck and on the upper back were significantly higher than those of the other four hair styles(p<.01). Mean skin temperature and rectal temperature had no significant differences among hair styles. Total body mass was the highest in the perm waved long hair style(PL)(p<.05). We acquired fundamental data to enable the improvement of the current hair clinic system, wigs, functional hats, and helmets.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.32 no.11
• /
• pp.1792-1801
• /
• 2008

This study was to develope the pesticide-proof clothes(PPC) for fruit-grower which has been well known over applied agricultural chemicals. The ergonomic evaluation of PPC were tested in two ways. Male adults volunteered the tests to evaluate the safety to pesticide in the field and the thermal comforts in the climate-chamber($30^{\circ}C$, 60%R.H.). PPC were made of 4 different fabrics. Two of them were on the market(coated non-woven and coated nylon). Others were water-repellent treatment and coated waterproof film by developed polyester. The field study was conducted for farmers growing apples to evaluate pesticide exposure. In this experiment, we collected data with patch test on the head, chest, back, right upperarm, right forearm, left thigh and left calf. From the results, the developed PPC showed the more excellent comfort than an existing PPC with nylon coated polyurethane. But the developed PPC of water-repellent fabric was penetrated into the PPC. Therefore, we designed the functional pesticide-proof clothes of 2 different developed polyester fabrics(water-repellent treatment in chest, abdomen, the lower of back, waist, and calf; coated waterproof film in head, shoulder, the upper of back, the crotch, hip, upper arm and thigh).

• Journal of Bio-Environment Control
• /
• v.29 no.3
• /
• pp.306-312
• /
• 2020

This study was conducted to investigate cucumber plants response to greenhouse environments by solar shading in greenhouse in the summer. In order to estimate heat stress reduction of cucumber plants by solar shading in greenhouse, we measured and analyzed physiological conditions of cucumber plants, such as leaf temperature, leaf-air temperature, rubisco maximum carboxylation rate, maximum electron transport rate, thermal breakdown, light leaf respiration, etc. Shading levels were 90% mobile shading of full sunlight, 40% mobile shading of full sunlight and no shading(full sunlight). The 90% shading screen was operated when the external solar radiation is greater than 650 W·m^-2. Air temperature, solar radiation, leaf temperature, leaf-air temperature and light leaf respiration in the 90% shading of full sunlight was lower than those of 40% shading and no shading. Rubisco maximum carboxylation rate, arrhenius function value and light leaf respiration of the 90% shading were significantly lower than those of 40% shading and no shading. The thermal breakdown, high temperature inhibition, of 90% shading was significantly higher than that of 40% shading and no shading. Therefore, these results suggest that 90% mobile shading made a less stressful growth environment for cucumber crops.

• Fashion & Textile Research Journal
• /
• v.1 no.2
• /
• pp.173-181
• /
• 1999

The purpose of this study was to investigate actual sleeping environments in Ondol rooms depending on the season. The experiment was performed on five healthy women. The bedroom environments using Ondol were measured in five cases (three apartments and two houses). The environments in bedroom, bedding temperature, skin temperature and thermal sensation were measured continuously through the seven days for each season in real life. This data of sleeping environments were analyzed in the view of seasonal variations and housing types. Annual average bedroom temperatures: $26.2{\sim}31.0^{\circ}C$ in apartments, $15.7{\sim}33.6^{\circ}C$ in houses. Annual average bedroom humidity: 48.3~82.1% RH in apartments, 64.9~87.0% RH in houses. During sleeping, temperatures of contact surfaces like sheets and under quilts ranged between $30.5^{\circ}C$ and $34.1^{\circ}C$ regardless of season or housing type. Annual average rectal temperature was $36.8^{\circ}C$ with no significant difference in season or housing type. In the point of thermal sensation, neutral temperature of the bedroom was $25.9^{\circ}C$ in apartments and $20.3^{\circ}C$ in houses. It was concluded that in spite of thermal environmental variations according to the seasons, skin, bedding and bedroom temperatures in apartments were better and more stable than those of houses. It is regarded that while houses are brick structured, apartments are steel-frame structured. Due to better insulation and air tightness, apartments were affected less from outdoor temperature and maintained higher room temperature than houses.

• Korean Journal of Remote Sensing
• /
• v.36 no.5_1
• /
• pp.667-677
• /
• 2020

Crop water stress can be detected based on soil moisture content, crop physiological characteristics and remote-sensing technology. The detection of crop water stress is an important issue for the accurate assessment of yield decline. The crop water stress index (CWSI) has been introduced based on the difference between leaf and air temperature. In this paper, drone-based thermal infrared image was used to map of crop water stress in water control plot (WCP) and water deficit plot (WDP) over spring chinese cabbage fields. The spatial distribution map of CWSI was in strong agreement with the abnormal growth response factors (plant height, plant diameter, and measured value by chlorophyll meter). From these results, CWSI can be used as a good method for evaluation of crop abnormal growth monitoring.

• Korean Journal of Environmental Biology
• /
• v.40 no.3
• /
• pp.247-254
• /
• 2022

To investigate the tolerance limit and critical thermal maximum (CTM), behavioral responses of wild goldeye rockfish Sebastes thompsoni according to exposure to high water temperature were observed using a continuous behavior tracking system. As a result, behavioral index (BI) of S. thompsoni in each temperature (20.0, 25.0, and 30.0℃) showed a significant difference (p<0.05) when compared with the value measured in a stable condition of 15.0℃. The activity level of S. thompsoni exposed to 25.0℃ decreased sharply after 20 hours. Their rest time at the bottom of experiment chamber increased, and their normal swimming and metabolic activities were disturbed. In addition, at a high water temperature of 30.0℃, S. thompsoni reached the limit of resistance and showed a sub-lethal reaction of swimming behavior, with energy consumption in the body increased and all test organisms died. In conclusion, the eco-physiological response of S. thompsoni to water temperature varied greatly depending on the fluctuation range of the exposed temperature and the exposure time. In addition, the tolerance limit of S. thompsoni to high water temperature was predicted to be 25.0-30.0℃. The maximum critical thermal that had a great influence on the survival of this species was found to be around 30.0℃.

• Fisheries and Aquatic Sciences
• /
• v.23 no.1
• /
• pp.1.1-1.14
• /
• 2020

Background: Dietary protein requirements are dependent on a variety of factors and water temperature is one of the most important abiotic factors affecting protein requirement of fish. This study was, therefore, conducted to investigate effects of water temperature on dietary protein requirement of fry Heteropneustes fossilis which has high demand in most of the Asian markets. Methods: Quadruplicate groups of 30 fish per treatment (2.97 ± 0.65 cm; 5.11 ± 0.34 g) were fed seven isoenergetic diets (17.9 kJ g^-1 gross energy; 14.99 kJ g^-1 digestible energy) containing dietary protein levels ranging from 28 to 52% at two water temperatures (18 and 26 ℃). Experimental diets were fed to apparent satiation as semi-moist cakes thrice daily at 17:00, 12:00, and 17:30 h for 12 weeks. For precise information, various growth parameters, protein deposition, hematological parameters, metabolic enzymes, and stress response were analyzed, and effects of water temperature on dietary protein requirement was recommended on the basis of response from above parameters. Results: Groups held at 26 ℃ attained best growth, feed conversion, and protein deposition at 44% dietary protein indicating that temperature affected dietary protein requirement for optimum growth of H. fossilis fry and protein requirement seems to be satisfied with 44% dietary protein. Interestingly, interactive effects of both dietary protein levels and temperature were not found (P > 0.05). Fish reared at 18 ℃ had comparatively higher values for aspartate and alanine transferases than those reared at 26 ℃ water temperature which exhibited normal physiological value for these enzymes indicating that body metabolism was normal at this temperature. Hematological parameters also followed same pattern. Furthermore, fish reared at 26 ℃ water temperature exhibited more resistant to thermal stress (P < 0.05). The 95% maximum plateau of protein deposition data using second-degree polynomial regression analyses exhibited dietary protein requirement of fry H. fossilis between 40.8 and 41.8% of diet at 26 ℃ water temperature. The recommended range of dietary protein level and protein/digestible energy ratio for fry H. fossilis is 40.8-41.8% and 27.21-27.88 mg protein kJ^-1 digestible energy, respectively. Conclusions: Information developed is of high significance for optimizing growth potential by making better utilization of nutrient at 26 ℃ and, to develop effective management strategies for mass culture of this highly preferred fish species.