• The Plant Pathology Journal
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• 제19권2호
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• pp.106-110
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• 2003

Stolbur Phytoplasma was detected from Lilium Oriental hybrids showing flattened stem and flower clustering. The presence of phytoplasma was demonstrated using polymerase chain reaction（PCR) assays with phyto-plasma-universal（P1/P6）and stolbur phytoplasma-specific 16F1/R1-S primer pairs amplifying phytoplasma 16S rDNA regions. Nucleotide suquences of the phytoplasma 16S rDNA were determined. Nucleic acid extracted from lily amplified 1.5 kb DNA with a phytoplasma universal primer pair. In nested PCR, 1.1 kb PCR product was obtained using specific primer pair, indicating an isolate of stolbur phytoplasma. Nucleotide sequence of phytoplasma 16S rDNA reported in this study showed 99.5% and 99.1％ identities with two known stolbur phytoplamas (16Sr XII-A). Also, it exhibited a sequence homology of 98.0％ with phormium yellow leaf (16Sr XII-B), and 97.9％ with Australian grapevine yellows (16Sr XII-B). Meanwhile, it showed 98.1% identity with strawberry green petal phytoplama, (16Sr1-C), and 94.7 % with American aster yellows (16Sr1-B). Homology percentage of the 16S rDNA nucleotide sequence suggests that this phytoplama could be classified into the stolbur phytoplasma, subgroup A (16Sr XII-A), as a type strain stolbur.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제13권4호
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• pp.1812-1824
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• 2019

The location and human activity are usually used as one of the important parameters to monitor the health status in healthcare devices. However, nearly all existing location and monitoring systems have the limitation of short-range communication and high power consumption. In this paper, we propose a new mechanism to collect and transmit monitoring information based on LoRa technology. The monitoring device with sensors can collect the real-time activity and location information and transmit them to the cloud server through LoRa gateway. The user can check all his history and current information through the specific designed mobile applications. Experiment was carried out to verify the communication, power consumption and monitoring performance of the entire system. Experimental results demonstrate that this system can collect monitoring and activity information accurately and provide the long rang coverage with low power consumption.

• 한국콘텐츠학회논문지
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• 제20권11호
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• pp.58-76
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• 2020

본 연구는 문화공간 시설을 대상으로 유니버설디자인의 현황과 문제점을 파악하고자 한다. 아울러 부산의 대표적 문화공간인 부산 시립박물관과 부산 시립미술관 그리고 부산 현대미술관을 선정하여, 유니버설디자인의 적용 여부 및 현황을 파악하고 바람직한 개선사항을 도출하고자 한다. 연구 내용으로는 첫째, 문헌고찰을 통해서 유니버설디자인의 개념 및 평가요소 등에 대한 전반적인 연구를 진행하였다. 둘째, 선행연구 분석을 통해 유니버설디자인의 5가지 평가요소를 도출하였다. 셋째, 현장조사를 통해, 각 문화공간을 직접 방문하고 그 실태를 조사한 후, 유니버설디자인의 현황 및 문제점을 파악하였다. 문화공간별 세부평가항목 결과를 보게 되면 그 순위는 부산 현대미술관, 부산 시립박물관, 부산 시립미술관 순으로 나타났고, 위 세 가지 장소에서 문화공간의 유니버설디자인의 평균 적용성은 53.6%로 나타났다. 또한, 시설별 평균은 매개시설이 가장 좋은 평가를 보였으며 다음으로 내부시설, 위생시설, 기타시설, 안내시설의 순으로 나타났다. 앞으로 공공 문화시설에 대해 정기적으로 유니버설디자인에 관한 조사 및 관련 시설의 개조가 필요하다고 여겨진다.

• International Journal of Oral Biology
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• 제35권1호
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• pp.21-25
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• 2010

This study was undertaken to develop species-specific forward and universal reverse PCR primers for the detection of Streptococcus sobrinus. These primers target the variable regions of the 16S ribosomal RNA coding gene (rDNA) and their specificity was tested against 10 strains of S. sobrinus strains and 20 different species of oral bacteria using serial dilutions of the purified genomic DNA of S. sobrinus ATCC $33478^T$. Our data show that species-specific amplicons were obtained from all the S. sobrinus strains tested but not from other species. Both direct and nested PCR could detect as little as 400 pg and 4 fg of genomic DNA from S. sobrinus ATCC $33478^T$, respectively. This result suggests that these PCR primers are highly specific and sensitive and applicable to the detection of S. sobrinus.

• The Plant Pathology Journal
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• 제19권5호
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• pp.221-226
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• 2003

Twenty universal rice primers (URPs) were used to detect PCR polymorphisms in 25 isolates of six different Alternaria species producing host specific toxins (HST). Eight URPs could be used to reveal PCR polymorphisms of Alternaria isolates at the intra- and inter-species levels. Specific URP-PCR polymorphic bands that are different from those of the other Alternaria spp. were observed on A. gaisen and A. longipes isolates. Unweighted pair-group method with arithmetic mean (UPGMA) cluster analysis using 94 URP polymorphic bands revealed three clustered groups (A. gaisen group, A. mati complex group, and A. logipes group).

• 한국멀티미디어학회논문지
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• 제22권11호
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• pp.1242-1250
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• 2019

Super resolution technique aims to convert a low-resolution image with coarse details to a corresponding high-resolution image with refined details. In the past decades, the performance is greatly improved due to progress of deep learning models. However, universal solution for various objects is a still challenging issue. We observe that learning super resolution with a general dataset has poor performance on faces. In this paper, we propose a super resolution fusion scheme that works well for both general- and face datasets to achieve more universal solution. In addition, object-specific feature extractor is employed for better reconstruction performance. In our experiments, we compare our fusion image and super-resolved images from one- of the state-of-the-art deep learning models trained with DIV2K and FFHQ datasets. Quantitative and qualitative evaluates show that our fusion scheme successfully works well for both datasets. We expect our fusion scheme to be effective on other objects with poor performance and this will lead to universal solutions.

• Asian Pacific Journal of Cancer Prevention
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• 제17권8호
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• pp.4117-4123
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• 2016

Background: Methylation of promoter 2 of the SHP1 gene is epithelial cell specific, with reported potential as a lymph node metastatic marker. Objective: To demonstrate SHP1-P2 methylation-specific quantitative PCR effectiveness in detecting colorectal cancer (CRC) DNA in lymph nodes. Materials and Methods: SHP1-P2 methylation levels were measured in lymph nodes of CRC patients and compared with pathological findings and patient prognosis. Results: Lymph nodes of CRC metastatic patients without microscopically detectable cancer cells had higher SHP1-P2 methylation levels than lymph nodes of controls (p<0.001). In addition, a higher SHP1-P2 methylation level was associated with a shorter duration until adverse disease events, metastasis, recurrence and death (r2 = 0.236 and p value = 0.048). Studying two cohorts of 74 CRC patients without microscopic lymph node metastases showed that only the cohort containing samples with high SHP1-P2 methylation levels had a significant hazard ratio of 3.8 (95%CI = 1.02 to 14.2). Conclusions: SHP1-P2 methylation PCR can detect CRC DNA in lymph nodes even if cancer cells are not visible under a microscope, confirming applicability as a potential universal lymph node metastatic marker.

• 대한인간공학회지
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• 제24권1호
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• pp.47-53
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• 2005

The purpose of this study was to evaluate performance and subjective satisfaction of mobile phone's interface elements considering the elderly users. First, mobile phone's interface elements were outlined recent foreign and domestic studies. Then, final three mobile phone's interface elements were determined by expert rating, AHP analysis, and questionnaire analysis. Objective and subjective measures were then used to determine the effects of several specific design parameters and aging differences on performance of mobile phones. In this experiment, 38 subjects and 4 independent variables were used to measure task completion time and user's subjective satisfaction. The statistical analyses indicated significant aging differences between older people and younger people in terms of keypad type and bell sound level in performance. Significant aging differences were also found for user subjective satisfaction. It is suggested that some major results from this study be used to develop mobile phone design guidelines that are more usable and accessible for most users, particularly for older users.

• IMMUNE NETWORK
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• 제21권4호
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• pp.28.1-28.14
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• 2021

Lung-resident memory T cells (T_RM) play an essential role in protecting against pulmonary virus infection. Parenteral administration of DNA vaccine is generally not sufficient to induce lung CD8 T_RM cells. This study investigates whether intramuscularly administered DNA vaccine expressing the nucleoprotein (NP) induces lung T_RM cells and protects against the influenza B virus. The results show that DNA vaccination poorly generates lung T_RM cells and massive secondary effector CD8 T cells entering the lungs after challenge infection do not offer sufficient protection. Nonetheless, intranasal administration of non-replicating adenovirus vector expressing no Ag following priming DNA vaccination deploys NP-specific CD8 T_RM cells in the lungs, which subsequently offers complete protection. This novel 'prime and deploy' strategy could be a promising regimen for a universal influenza vaccine targeting the conserved NP Ag.

• 한국컴퓨터정보학회논문지
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• 제7권1호
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• pp.103-111
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• 2002

XMAN은 3-계층 구조를 가지며, 웹, XML 및 서버 측 자바 기술들의 확장에 기반하여 구현된다. Currently. network managers are making use of expensive, vendor-specific management consoles to view and manipulate network data. And more and more systems are transferred to web environment. Therefore, network managers want to get information at anywhere, anytime. This requires an access of management information to be unified, cost-effective, and easy-to-use. This paper presents the implementation of an XML-based universal network management system (XMAN), which supports multiple internet devices as management console. XMAN has 3-tire architecture, and the implementation is based on an extensive use of emerging Web, XML, and Java technologies.