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A "Prime and Deploy" Strategy for Universal Influenza Vaccine Targeting Nucleoprotein Induces Lung-Resident Memory CD8 T cells

  • Haerynn Chung (Graduate School of Pharmaceutical Sciences, Ewha Womans University) ;
  • Eun-Ah Kim (Graduate School of Pharmaceutical Sciences, Ewha Womans University) ;
  • Jun Chang (Graduate School of Pharmaceutical Sciences, Ewha Womans University)
  • Received : 2020.11.30
  • Accepted : 2021.08.13
  • Published : 2021.08.31
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Abstract

Lung-resident memory T cells (TRM) play an essential role in protecting against pulmonary virus infection. Parenteral administration of DNA vaccine is generally not sufficient to induce lung CD8 TRM cells. This study investigates whether intramuscularly administered DNA vaccine expressing the nucleoprotein (NP) induces lung TRM cells and protects against the influenza B virus. The results show that DNA vaccination poorly generates lung TRM cells and massive secondary effector CD8 T cells entering the lungs after challenge infection do not offer sufficient protection. Nonetheless, intranasal administration of non-replicating adenovirus vector expressing no Ag following priming DNA vaccination deploys NP-specific CD8 TRM cells in the lungs, which subsequently offers complete protection. This novel 'prime and deploy' strategy could be a promising regimen for a universal influenza vaccine targeting the conserved NP Ag.

Keywords

Acknowledgement

We are grateful to the members of the immunology laboratory for their helpful discussion and technical assistance. The authors wish to thank all members of the HYEHWA FORUM for their helpful comments and creative motivation. This study was supported by a grant of the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (grant No. HV20C0049).

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