• Korean Journal of Plant Tissue Culture
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• v.28 no.2
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• pp.103-108
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• 2001

The in vitro plantlets of cassava (Manihot esculenta Crantz cv. MColl 22) could be regenerated from nodal explant cultures in a liquid MS basal medium containing 0.01 mg/L zeatin for 2 weeks. The plantlets of 1.5∼2.5 cm in shoot length were transplanted to a glass bottle containing fine sand and acclimated under non-sterile conditions after excising their intact roots by: 1) prune leaving roots base of 1∼1.5 cm; 2) complete removal of roots; and 3) cutting off the rooting zone. The majority of in vitro-formed intact roots continued growth after transferred to soil, and all of the damaged roots stopped further growth. The plantlets with excised roots began to develop new roots within 7∼10 days after being transferred to a glass bottle, and a few of the pruned roots developed lateral roots from the remaining portion. Pruning and removal of in vitro roots resulted in a high survival rate (>87%), and did not significantly affect ex vitro root regeneration and acclimation, but the plantlets in which the rooting zone had been cut-off showed 73% survival rate. Pruning or removal of in vitro roots before transfer of plantlets is recommended for useful method of commercial micropropagation because of easier handling and high survival rate of plantlets.

• Applied Biological Chemistry
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• v.9
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• pp.119-124
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• 1968

In order to learn the effect of timing and rate of potash fertilization in the rice plant nursery on the root growth and the vegetative growth of later stage a pot experiment was carried out employing 'Jaekun' a rice variety. Potassium Chloride was applied at the rate of $0{\sim}100g\;K_{2}O/3.3m^2$ right before, and 30 days after seeding. Forty-day old seedlings were transplanted three times successively at the one week intervals with the roots cut each time. Each subject was observed in rooting and other useful traits. A part of seedlings after the third root scission were cultured intact thereafter and the yield characters examined. The results were shown as: 1. The rate of sound seedlings was high in the case that a small amount of potassium was applied as the basic placement while the application of the fertilizer over $75g/3.3m^2$ (as $K_{2}O$) yielded a far less crops. 2. The plant height, number of tillers, number of leaves, and grass weight increased as the amount of potassium was elevated. However, the application of $50g\;K_{2}O/3.3m^2$ in the basic placement and that of $75g\;K_{2}O/3.3m^2$ were the peaks over which the above mentioned characters were weakened. 3. As the amount of potassium was increased the increment in number of rooting was notable, especially when the scission of roots was repeated within the limit of $50{\sim}75g\;K_{2}O/3.3m^2$. 4. In the maximum root newly shooted length indicated was about the same tendency although no statistical significance was observed. 5. The plant height, number of tillers, and variation of weight between grass and root indicated a similar tendency as the number of root shoot. 6. The differences in number of ears, grain yield, and weight of straw between the treatments were not so great but showed somewhat similar trend as in the growth of transplanted plants. In the treatment-100g $K_{2}O/3.3m^2$ the yield (ears, grain and straw) decreased as in the non-fertilized.

• Journal of the Korean Institute of Landscape Architecture
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• v.21 no.3
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• pp.99-104
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• 1993

The objectives of this study were investigation of the sutiable propagation method by softwood cuttings of female Ilex serrata, the comparison of sex determination of Ilex serrata after seeding and the fruiting percentage of the Ilex serrata male and female. The results obtained were as follows. 85 percentage of the seed were germinated with three months cold stratification. 35 percentage of female plant were observed after seeding. Cuttings should be rooted on late June using a quick dipping of 1,000ppm IBA solution. The rooting rate of the softwood cuttings treated IBA 1,000pm on June 22 in a closed moist chamber with 50% shade screen was 72.2 percentage. The suitable planting of male and female of Ilex serrata was male one versus female thirteen in a distance of 50 meter.

• Journal of Plant Biotechnology
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• v.5 no.2
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• pp.115-119
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• 2003

To determine the appropriate concentrations of nutrients and growth regulators for shoot proliferation and root initiation, several rose hybrid tea cultivars were cultured. Cultured shoot tips and lateral buds from different cultivars proliferated multiple shoots on Murashige and Skoog (MS) medium supplemented with 0 to 4 mg/L BA and 0 to 0.05 mg/L NAA. The ability of the explants to proliferate shoots and initiate roots was affected by genotype, the nodal position of explant, the strength of MS basal medium and growth regulators used. The buds nearest the apex exhibited the slowest rate of development. Most cultivars had the highest shoot proliferation when cultured on MS medium with 2 mg/L BA and 0.01 mg/L NAA, but the degree varied by cultivars. Root development was enhanced by lowering the concentration of MS salts.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.58-58
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• 2020

Xanthoceras sorbifolium Bunge (yellowhorn) is a woody tree in the soapberry family, Sapindaceae, native to northern China. This species has been identified as a major woody bioenergy plant for bio-diesel production because of high oil content in seed. But the flowers do not bear fruit well while the many flowers blooming. This study was performed to regenerate in vitro plantlet using adventitious shoot formation. To establish the protocol of plant regeneration, adventitious shoots formation rate in the culture of cotyledon of immature zygotic embryos was 68.6% in 1/2 MS medium with 0.5 mg l-1 BA and 3% sucrose (w/v). In the culture of cotyledons of mature zygotic embryos, induction of adventitious shoots was needed to contain high sucrose in pre-culture medium and the frequency of shoot induction was 64.4%. Multiple shoots were induced in 0.5 mg l-1 TDZ, and rooting of shoot was induced 4.0 mg l-1 IBA. Flow cytometry analysis revealed that all the regenerated plantlets were diploid.

• Plant Resources
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• v.7 no.1
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• pp.1-6
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• 2004

In vitro proliferation system was achieved by using nodal segment excised from greenhouse grown juvenile stock plants of Alnus japonica. Stem explants were cultured on MS medium supplemented with different plant growth regulators of cytokinin and/or their combinations. The most effective cytokinin source was the combination of zeatin 2.0 mg/L and TDZ 0.05 mg/L producing the average number of shoots (16.8 $\pm$ 3.6). In addition, healthy roots were formed after small clumps of shoots were transferred to half strength of MS medium containing IBA 0.02 mg/L with optimal rooting capacity. Soil acclimatization was successfully conducted in cell tray containing artificially mixed soil with 92 % survival rate.

• Korean Journal of Plant Resources
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• v.7 no.1
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• pp.17-22
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• 1994

This study was carried out to investigate the optimum medium and concentrations of growth regulators for induction of multiple shoot by meristem culture of floe otorefcenf Mill. MS medium supple-mented with 3${\mu}{\textrm}{m}$ TDZ was effective for induction of multiple shoot. Shoot multiplication was more ef-fective when 2mg/1 BA combined with 0.Img/1 IAA than when only BA were treated on medium. Halfstrength of MS medium supplemented with 2mg/L IAA was effective for rooting of shoots regenerated.When plantlets regenerated from meristem culture were transferred to pot, survival rate of plantletswas 80% on perlite and was 95% on vermiculite, respectively.

• Journal of Bio-Environment Control
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• v.11 no.1
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• pp.18-22
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• 2002

Diseases occurrence of hydroponic culture was less than that of soil culture, especially gummy stem blight, which was 39.9-53.3% of infection rate at soil culture, was not almost observed at hydroponic culture. Development of fermented fruit was higher at Shintozoa, Hongtozoa and self-rooting seedling in order at soil cultured but critically dropped at hydroponic culture without rootstock sort. Therefore proper moisture management at root zone can decrease the development of fermented fruit.

• Journal of Bio-Environment Control
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• v.6 no.4
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• pp.292-298
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• 1997

These studies were conducted to verify the effect of the supplementary lighting by reflective film mulching and its establishment in the north side of greenhouse on the utility of light at tomato by means of investigating changes of leaf temperatures, stomatal features, transpiration rates and photosynthetic rates. Stomatal density of leaves were high in the reflective film mulching but sizes of stomata were not different. As the osmotic potential in rooting zone was low, the stomatal resistance was high, transpiration rate was low, and leaf temperature was increased by 40.62$^{\circ}C$. And also in the block of reflective film mulching photosynthetic rates were decreased hut chlorophyll contents were not different. Especially, there is an effect of controlling greenhouse whiteflies by treatment of reflective film mulching. It is thought that the reason of high quality or increasing yield at several crops by supplementary lighting, such as reflective film mulching, would be caused by influences of absorption and distribution of nutrients through high transpiration rate and photosynthesis which resulted from increase of stomata.

• Journal of the Korean Institute of Landscape Architecture
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• v.27 no.4
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• pp.50-58
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• 1999

This study was carried out to investigate the growth characteristics and propagation methods of L. obtusiloba as a woody landscape plant. The results are obtained as follows: L. obtusiloba was dioecious shrub and shaped with bush type. Leaves were ovate, obtuse, cordate, 7.05 cm long, 7.20 cm wide, and petiole 2.0 cm long. Flowers of L. obtusiloba were diclinous. Soil acidity ranged from pH 4.06 to 5.53 with the lowest at the Mt. Soo-Ri. Mt. Soo-Ri located near factory district, which was considered to damaged by environmental deteoration. While soil organic matter was highest at Mt. Soo-Ri, inorganic nutrients were low. L. obtusiloba grows in the area with low soil acidity and low content of inorganic. Therefore it seemed to be tolerant to air pollution. L. Obtusiloba was high seed germination rate in the plug box and its shoots were longer than seeding box and softwood cutting of L. obtusiloba showed the rooting rate of 50% at 5,000ppm on June 23. To develop a mass propagation method of Korean native L. obtusiloba through an axillary bud culture as a woody landscape plant, about 2∼3 cm shoots induced from explant were subcultured to new media contained different growth regulators. Shoots multiplied most effectively on a WPM containing 1.0 mg/l BA, producing 5.5 shoots with a shoot length of 2.5 cm per shoot explant.