• Journal of Food Hygiene and Safety
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• v.26 no.1
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• pp.16-24
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• 2011

A method was established for the simultaneous determination of sugar alcohols, erythritol, xylitol, sorbitol, inositol, mannitol, maltitiol, lactitol and isomalt by High Performance Liquid Chromatography (HPLC). The sugar alcohols were converted into strong ultraviolet (UV)-absorbing derivatives with p-nitrobenzoyl chloride (PNBC). HPLC was performed on Imtakt Unison US-$C_18$ column, using acetonitrile: water (77:23) as a mobile phase and UV detection (260 nm). The calibration curves for all sugar alcohols tested were linear in the 10~200 mg/L range. The average recoveries of the sugar alcohols from three confectioneries spiked at 100 ppm of eight sugar alcohol standards ranged from 81.2 to 123.1% with relative standard deviations ranging fromo 0.2 to 4.9%. The limits of detection (LODs) were $0.5{\sim}8\;{\mu}g/L$ and the limits of quantification (LOQs) were $2{\sim}17\;{\mu}g/L$. Reproducibility of 8 sugar alcohols was 0.28~1.97 %RSD. The results of the analysis of confectioneries showed that 89 samples of 130 were detected and the sugar alcohols content of samples investigated varied between 0.4 and 693.7 g/kg. A method for the simultaneous determination of eight sugar alcohols will be used as basic data for control of sugar alcohols in confectioneries, and quality control in food manufacturing.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.2
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• pp.166-173
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• 2010

In order to obtain the basic information for agricultural utilization of germanium (Ge), the growth characteristics, Ge uptake, and grain quality of rice plant (Hopyungbyeo) were investigated under different germanium ($GeO_2$, and commercial Ge) treatments in paddy field. Phytotoxicity was detected in $GeO_2$ treatment but not in commercial Ge treatment. The grain yield was greater in the order of control treatment > commercial Ge treatment > $GeO_2$ treatment. The dry weight was greater in order of control treatment > $GeO_2$ treatment ${\geq}$ commercial Ge treatment. The Ge content of leaf in $GeO_2$ treatment was 6 times (177 mg $m^{-2}$) higher than that in commercial Ge treatment. The Ge content in rice bran was not different in $GeO_2$, and commercial Ge treatments. The Ge contents of brown rice in$GeO_2$, and commercial treatments were 40.9, and 31.1 mg $kg^{-1}$, respectively. The Ge uptake rates in rice plant was higher in the order of leaf > rice bran > brown rice > stem > root. Under $GeO_2$, 15.56% of Ge absorbed into plant with 11.1% in leaf, 1.6% in stem, 0.03% in root, 2.2% in rice bran and 0.73% in brown rice. Under commercial Ge treatment, 5.19% of Ge absorbed into plant with 1.8% in leaf, 0.46% in stem, 0,01% in root, 2.2% in rice bran, and 0.71% in brown rice. Based on these results, the Ge contents in polished rice in commercial Ge treatment were higher than those in $GeO_2$ treatment. However, the Ge contents of rice grain (containing rice bran and polished rice) in $GeO_2$ treatment were higher than those in commercial Ge treatment.

• Journal of Bio-Environment Control
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• v.12 no.3
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• pp.152-159
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• 2003

This study was conducted to analyze the water consumption in closed transplants production system (CTPS) for the production of quality transplants and to investigate the effect of relative humidity on the water balance in CTPS. Potato (Solanum tuberosum L. cv. Dejima) plug seedlings were grown for 15 days at air temperature of 20$^{\circ}C$, relative humidity of 70%, photoperiod of 16/8 h, and photosynthetic photon flux (PPF) of 200 ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-l}$ following rooting for 5 days in CTPS. Amount of humidified, dehumidified, irrigated and evapotranspirated water were 67.9 kg${\cdot}m^{-2},\;196.9{\cdot}m^{-2},\;44.3\;kg{\cdot}m^{-2},\;33.5\;kg{\cdot}m^{-2}$, respectively. Water content of media and plants were 1.2 kg${\cdot}m^{-2},\;6.9\;kg{\cdot}m^{-2}$, respectively. Three relative humidity levels of 60, 70, and 80% were provided to analyze the effect of humidity on the water balance in CTPS. Amount of humidified, dehumidified, irrigated, evapotranspiratad water and water contents of media and plants increased with increasing relative humidity. Since the water consumption required to produce plug seedlings in CTPS dec1eased with decreasing relative humidity, the available water utilization efficiency of CTPS increased with decreasing relative humidity. CTPS showed high available water utilization efficiency of 0.92 - 0.97 if dehumidified water in CTPS was recycled. The development of CTPS with recycling system of dehumidified water will not only reduce the water consuming for the production of transplants but contribute to the establishment of plant production economizing in water consumption.

• Clinical and Experimental Reproductive Medicine
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• v.35 no.3
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• pp.203-212
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• 2008

Objective: During cryopreservation process, cold shock and cryo-injury affect the fertilizing capacity of the sperm by damaging cell membranes with loss of functional integrity. A longstanding concept for preventing the cryo-damage is to stabilize the plasma membrane by incorporating cholesterol. This study was to determine the effects of cholesterol in freezing media on the motility and functional integrity of human sperm after cryopreservation. Methods: Control group (non-cholesterol treated) and different concentrations of cholesterol-treated sperm (14 healthy males) were frozen and thawed. After freezing and thawing of sperm, the quality of sperm was evaluated by sperm analysis, acrosome reaction test and sperm chromatin structure assay. Results: When human sperm were incubated in sperm freezing medium (SFM) containing $0.5{\mu}g$ cholesterol and then freezing/thawing, the motility of sperm have significantly improved compared to those untreated cholesterol ($33.46{\pm}1.48%$ vs. $30.10{\pm}1.07%$, p<0.05). The rate of calcium ionophore-induced acrosome reactions in post-thawed sperm was significantly higher than that ($53.60{\pm}1.60%$ vs. $47.40{\pm}1.86%$, p<0.05) in SFM containing cholesterol. Sperm chromatin structure assay revealed that DNA damage to the sperm in the cholesterol-treated group was lower than that of non-treated group. Conclusion: These results suggest that increased cholesterol content of sperm plasma membrane by supplementation of cholesterol in SFM improves sperm motility, capacitation status, and DNA integrity. Therefore, addition of cholesterol into SFM could be a useful for protecting human sperm from cold shock and cryo-injury during cryopreservation.

• Asian Journal of Turfgrass Science
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• v.22 no.2
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• pp.161-170
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• 2008

Recently irregular dark-colored patches were found on the Kentucky teeing ground in a golf course in Gyunggi providence. Interestingly, blue-green algae from the leaf tissue sample containing black spot-stained symptoms were largely observed through microscopic study. In general, algae present on the upper soil surface or in the upper layer of root zone form dark brown layers of scum or crust, which invoked harmful effects to turf growth such as poor drainage, inhibition of new root development. In this observation, unlike the algae were sometime found in senescing leaves on contacted soil in July and August, the blue-green algae were detected within black spot-stained Kentucky bluegrass leaf tissues including leaf blade, ligule, auriclea as well as leaf sheath. The blue-green algae were also detected on the leaf and stem tissue adjacent to the symptomatic leaf tissues. Two species of blue-green algae, Phomidium and Oscillatoria, were greatly observed. Oscillatoria species was more commonly notified in all samples. In addition, the two species were found on a putting green showing yellow spot disease at another golf course in Gyunggi providence. The data from chemical control assay revealed that chemicals such as propiconazole, iprodione, and azoxystrobin decreased blue-green algae population and leaf spots, which finally resulted in enhanced leaf quality. All taken together, we strongly suggested that the disease-like phenomenon by blue-green algae might be very closely mediated with infection/translocation process in relation with turfgrass. It indicates that blue-green algae in turf management may play an adverse role as a secondary barrier as well as a pathogenic agent. This report may be helpful for superintendents to recognize and understand the fact that algae control should be provided more cautiously and seriously than we did previously in upcoming golf course management.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.4
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• pp.459-464
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• 2017

Biphenyl is used as an intermediate in the production of crop protection products, a solvent in pharmaceutical production, and as a component in the preservation of citrus fruits in many countries. Biphenyl is not authorized for use and also does not have standards or specifications as a food additive in Korea. National and imported food products are likely to contain biphenyl. Therefore, control and management of these products is required. In this study, a simple analytical method was developed and validated using HPLC to determine biphenyl in food. These methods are validated by assessing certain performance parameters: linearity, accuracy, precision, recovery, limit of detection (LOD), and limit of quantitation (LOQ). The calibration curve was obtained from 1.0 to $100.0{\mu}g/mL$ with satisfactory relative standard deviations (RSD) of 0.999 in the representative sample (orange). In the measurement of quality control (QC) samples, accuracy was in the range of 95.8~104.0% within normal values. The inter-day and inter-day precision values were less than 2.4% RSD in the measurement of QC samples. Recoveries of biphenyl from spiked orange samples ranged from 92.7 to 99.4% with RSD between 0.7 and 1.7% at levels of 10, 50, and $100{\mu}g/mL$. The LOD and LOQ were determined to be 0.04 and $0.13{\mu}g/mL$, respectively. These results show that the developed method is appropriate for biphenyl identification and can be used to examine the safety of citrus fruits and surface treatments containing biphenyl residues.

• Proceedings of the Korean Information Science Society Conference
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• 1998.10a
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• pp.480-482
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• 1998

최근, 멀티캐스트 기법을 사용하는 멀티미디어 응용 프로그램들이 인터넷에 등장하고 있다. 이들 응용 프로그램들의 성공 여부는 수신자들에게 전송되는 음성/영상의 품질에 의해 좌우된다. 인터넷은 응용프로그램의 QoS(Quality of Service) 에 대한 요구를 보장할 수 없기 때문에 멀티케스트 트래픽(multicast traffic)을 위하여 인터넷의 성능을 최대한 효율적으로 이용할 수 있도록 흐름제어에 대한 많은 연구가 진행되고 있다. 그 중 IVS(INRIA Video conferencing System)에서 제안한 멀티캐스트 트래픽 흐름제어 알고리즘은 수신자가 주기적으로 전달하는 RTCP 의 패킷손실 정보에 의해 송신자가 전송율을 조절하는 것이다. 그러나 이 알고리즘은 네트워크 상태가 무부하(unload)임에도 불구하고 느린 피드백으로 인하여 가용 네트워크 대역폭을 빠르게 파악하지 못하기 때문에, TCP트래픽과 경쟁 상태에서 네트워크 대역폭을 불공정(unfairness)하게 사용하게 되고 네트워크 상태에 알맞는 전송율을 결정하지 못한다. 본 논문에서는 더욱 공정하게 대역폭을 공유할 수 있고 전체 링크 이용율을 높이는 두 가지 기법을 제안한다. 첫째, 측정된 네트워크 혼잡상태에 따라 RTCP 피드백의 전송 빈도를 동적으로 조절하는 것이다. 둘째, TCP와 같이 전송율을 증가/감소시킴으로써 공정하게 네트워크를 공유하도록 하는 것이다. 본 논문에서는 이 두 가지 기법들이 TCP 트래픽에 영향을 주지 않고 또한 RTCP피드백의 양을 증가시키지 않으면서도 공정하게 네트워크 대역폭을 공유함으로써 링크의 이용율을 높일 수 있다는 것을 시뮬레이션을 통하여 보여준다.안 모니터링 기 능 등으로 조사되었다.도 멜-켑스트럼을 사용한 경우 67.5%, K-L계수를 사용한 경우 75.3%로 7.8%의 향상된 인식률을 보였으며 K-L계수와 회귀계수를 결합한 경우에서도 비교적 높은 인식률을 보여 숫자음에 대해서도 K-L계수의 유효성을 확인할 수 있었다..rc$ 구입할 때 중점적으로 살펴보는 사항은 신선도와 순수재래종 여부, 위생상태였다. 한편 소비자가 언제나 구입할 수 없다는 의견이 85.2%나 되어 원활한 공급과 시장조성이 아직 정착되지 않고 있었다. $\bigcirc$ 현재 유통되고 있는 재래종닭은 소비자 대부분이 잡종으로 인식하고 있었으며, 재래종과 일반육계와의 구별은 깃털색, 피부색, 정강이색등 외관상으로 구별하고 있었다. 체중에 대한 반응은 너무 작다는 의견이었고, 식품으로의 인식도는 비교적 고급식품으로 인식하고 있다. $\bigcirc$ 재래종닭고기의 브랜드화에 대한 견해는 젊고 소득이 높은 계층에서 브랜드화의 필요성을 강조하고 있다. $\bigcirc$ 재래종달걀의 소비형태는 대부분의 소비자가 좋아하였으나 아직 먹어보지 못한 응답자가 많았다. 재래종달걀의 맛에 대해서는 고소하고 독특하여 차별성을 느끼고 있었다. $\bigcirc$ 재래종달걀의 구입장소는 계란판매점(축협.농협), 슈퍼, 백화점, 재래닭 사육 농장등 다양하였으며 포장단위는 10개를 가장 선호하였고, 포장재료는 종이, 플라스틱, 짚의 순으로 좋아하였다. $\bigcirc$ 달걀의 가격은 200원정도를 적정하다고 하였으며, 크기는 (평균 52g)는 가장 적당하다고

• Proceedings of the Korea Water Resources Association Conference
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• 2005.05b
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• pp.454-458
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• 2005

여름철 집중강우시 유입되는 고탁수층은 저수지의 밀도성층으로 인하여 표수층 하부에 위치하며, 이를 적기에 배제하지 않을 경우에는 수평방향의 확산현상과 연직방향의 전도현상으로 인해 저수지 전역에 분포하게 되어, 탁수현상의 장기화를 유발한다. 일본 사메우라댐의 경우에는 1977년 9월 태풍 17호에 의한 집중호우로 대량의 고탁수가 유입되어 하류하천으로의 댐 방류수의 고탁도현상이 장기간 지속된 바 있다. 이와 같은 탁수장기화에 대한 저수지내 대책의 하나로서 홍수유입후 탁도가 높은 물을 단기간동안 방류하고, 갈수기에는 탁도가 낮은 물을 방류하는 선택취수 개념이 제안된 바 있다. 국내의 임하댐의 경우에도 2002년 태풍 '루사'와 2003년 태풍 '매미'로 인해 발생한 탁수가 전도현상을 거치며 저수지내 전수층에 확산됨으로써 댐방류수의 고탁도현상이 장기화된바 있다. 이로 인하여 댐하류에 위치한 안동시 용상정수장의 동절기 정수처리 장애로 2003년 11월이후 약 160여일동안 댐방류가 중단된 바 있다. 임하댐의 경우에도 탁수장기화에 대한 대책으로서 홍수기 고탁수층을 우선배제한 후, 저수지내에 유입되는 청수을 담수함으로써 이를 방지할 수 있다. 이러한 선택취수 기법은 동일한 수리동역학적 개념을 적용하여 하류하천의 냉해문제를 대비하기 위한 방법으로 표층의 온수를 선택방류하는 데에도 이용될 수 있다. 이와 같은 선택취수를 효율적으로 수행하기 위해서는 취수시설에 접근하는 밀도흐름을 정확하게 이해하고 있어야 한다. 또한 취수탑 인근에서의 온도 및 탁도 등의 연직분포를 실시간으로 파악할 수 있는 자동수질측정시스템이 구축되어 있어야 한다. 현재 임하댐의 경우, 하류하천의 수질을 고려한 효율적인 탁수배제를 위하여 상류 유입부 2개소, 발전취수탑 전면 1개소, 조정지댐 1개소 등 총 4개의 자동수질측정시스템이 설치되어 탁도와 온도가 실시간으로 모니터링되고 있으며, 향후 강우시 저수지에서의 밀도흐름을 보다 정밀하게 모니터링하기 위하여 저수지내 4개의 주요지점에 추가적으로 설치될 예정이다. 이와 같은 자동측정시스템과 더불어 주기적인 세부 수질조사를 실시하면 저수지내의 온도성층 변화와 탁수층 분포를 매우 정확하게 파악할 수 있으며, 호소의 수질모형과 연계하여 강우시 탁수 도달시간과 탁수량을 사전에 예측할 수도 있다. 또한 이와 같은 기초적인 정보를 바탕으로 하류하천의 탁수 피해를 최소화할 수 있는 선택취수탑의 운영방안을 수립할 수 있다 본 연구에서는 이를 위해 선택취수탑 주위의 성층흐름을 기존의 실험자료와 수치해석을 통하여 분석하였고, 온도성층구조나 취수구의 위치변화에 따른 방류수 수질특성을 조사하였다.

• Journal of Korean Medical classics
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• v.23 no.4
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• pp.63-102
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• 2010

Purpose : To propose various types of clinical research which is feasible for botanical new drug (IND) development processes, and suggest essential steps to development of study protocol for IND. Methods : Literature-based discussions and one research group's experience is given regarding domestic act, regulation, and system. Results : In order to get an approval of IND for botanical drug in Korea there are several types of clinical research to conduct. In quality control steps for standardized medicinal herbs, case reports or case series can be conducted, and for good manufacturing practice(GMP) steps, we can conduct case reports, case series, and retrospective cohort studies. In addition, as long as we gathered good laboratory practice(GLP) data we can conduct up to quasi-experimental studies and clinical trials including investigator initiated trials. In order to conduct these studies development of study protocol is essential. First, we obtain historical evidence including target disease and indication, efficacy, safety, and endpoints by reviewing medical classics. Second, we obtain clinically and statistically important data by conducting non-clinical studies, observation studies, and quasi-experimental studies. Third, we generate research hypotheses and purposes and explore methodologies, endpoints, clinical practice guidelines, cost-effectiveness, and commercial potential. Finally, we develop study protocol with aid of biostatistician or expert in contract research organization. Discussions and conclusions : This study have obvious limitations in that most thoughts, suggestions, and proposes are from one research group's experience. Therefore, we hope to see various types of research in this topic and process from other research group as well.

• Korean journal of food and cookery science
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• v.21 no.5
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• pp.717-724
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• 2005

Sensory characteristics and antimicrobial activity of Korean wheat noodle with pine pollen powder and antioxidant activity of pine pollen extracts were investigated to develop the health promoting product. Pine pollen powder was extracted with water and 70% ethanol and extracts were tested electron donating ability (EDA) and nitrite scavenging ability (NSA). Sensory characteristics were evaluated by its color, flavor, moisture, softness, texture and taste. Microbiological quality was tested by total viable cells. EDA was highest in 1,000 ppm for both water extract (67% of EDA) and ethanol extract (74% of EDA). NSA was highest in pH 1.2 for both water extract(46% of NSA) and ethanol extract (48% of NSA). Antimicrobial activity of Korean wheat noodle with 3% pine pollen powder were 0.5 log $cycle{\sim}1$ log cycle lower than that of control at 5days of storage in total vival cells and at 4days of storage in total fungus. From sensory evaluation, Korean wheat noodle with 3% pine pollen powder had significantly higher of all scores.