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Simultaneous Determination of Eight Sugar Alcohols in Foodstuffs by High Performance Liquid Chromatography  

Lim, Ho-Soo (Food Additives and Packages Division, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration)
Park, Sung-Kwan (Food Additives and Packages Division, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration)
Kwak, In-Shin (Food Additives and Packages Division, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration)
Kim, Hyung-Il (Food Additives and Packages Division, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration)
Sung, Jun-Hyun (Food Additives and Packages Division, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration)
Choi, Jung-Yoon (Korea Food Research Institute, Korea Foods Industry Association)
Kim, So-Hee (Food Additives and Packages Division, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration)
Publication Information
Journal of Food Hygiene and Safety / v.26, no.1, 2011 , pp. 16-24 More about this Journal
Abstract
A method was established for the simultaneous determination of sugar alcohols, erythritol, xylitol, sorbitol, inositol, mannitol, maltitiol, lactitol and isomalt by High Performance Liquid Chromatography (HPLC). The sugar alcohols were converted into strong ultraviolet (UV)-absorbing derivatives with p-nitrobenzoyl chloride (PNBC). HPLC was performed on Imtakt Unison US-$C_18$ column, using acetonitrile: water (77:23) as a mobile phase and UV detection (260 nm). The calibration curves for all sugar alcohols tested were linear in the 10~200 mg/L range. The average recoveries of the sugar alcohols from three confectioneries spiked at 100 ppm of eight sugar alcohol standards ranged from 81.2 to 123.1% with relative standard deviations ranging fromo 0.2 to 4.9%. The limits of detection (LODs) were $0.5{\sim}8\;{\mu}g/L$ and the limits of quantification (LOQs) were $2{\sim}17\;{\mu}g/L$. Reproducibility of 8 sugar alcohols was 0.28~1.97 %RSD. The results of the analysis of confectioneries showed that 89 samples of 130 were detected and the sugar alcohols content of samples investigated varied between 0.4 and 693.7 g/kg. A method for the simultaneous determination of eight sugar alcohols will be used as basic data for control of sugar alcohols in confectioneries, and quality control in food manufacturing.
Keywords
Sugar alcohols; HPLC; p-Nitrobenzoyl chloride;
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