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Characterization of the Extracellular ${\beta}-Galactosidase$ Produced from Streptomyces sp. YB-9 (Streptomyces sp. YB-9가 생산하는 균체외 ${\beta}-galactosidase$의 특성)

  • Lee, Kyung-Seop;Kim, Chang-Jin;Yoon, Ki-Hong
    • Applied Biological Chemistry
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    • v.46 no.4
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    • pp.299-304
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    • 2003
  • A strain YB-9 was isolated from soil as a producer of the extracellular ${\beta}-D-galactosidase$, which catalyzes the hydrolysis of lactose. The strain YB-9 was identified as Streptomyces sp. on the basis of its cultural, morphological and physiological properties. After treating culture supematant of the isolate with ammonium sulfate $(15{\sim}70%)$, the precipitated protein was used as a crude ${\beta}-galactosidase$ for analyzing its reaction properties with $para-nitrophenyl-{\beta}-D-galactoside$ $(pNP-{\beta}Gal)$ and lactose as substrates. The {\beta}-galactosidase showed its maximal activity at pH $6.0{\sim}6.5$ and $60^{\circ}C$. The hydrolyzing activity of ${\beta}-galactosidase$ for both $pNP-{\beta}Gal$ and lactose was decreased by galactose. Its hydrolyzing activity for lactose was slightly decreased by glucose, but the activity for $pNP-{\beta}Gal$ was increased to 1.3-folds by glucose. Especially, its hydrolyzing activity was not affected for lactose and was increased to 1.6-folds for $pNP-{\beta}Gal$ by xylose.

Experiment and Simulation for Evaluation of Jena Storage Plug-in Considering Hierarchical Structure (계층 구조를 고려한 Jena Plug-in 저장소의 평가를 위한 실험 및 시뮬레이션)

  • Shin, Hee-Young;Jeong, Dong-Won;Baik, Doo-Kwon
    • Journal of the Korea Society for Simulation
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    • v.17 no.2
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    • pp.31-47
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    • 2008
  • As OWL(Web Ontology Language) has been selected as a standard ontology description language by W3C, many ontologies have been building and developing in OWL. The lena developed by HP as an Application Programming Interface(API) provides various APIs to develop inference engines as well as storages, and it is widely used for system development. However, the storage model of Jena2 stores most owl documents not acceptable into a single table and it shows low processing performance for a large ontology data set. Most of all, Jena2 storage model does not consider hierarchical structures of classes and properties. In addition, it shows low query processing performance using the hierarchical structure because of many join operations. To solve these issues, this paper proposes an OWL ontology relational database model. The proposed model semantically classifies and stores information such as classes, properties, and instances. It improves the query processing performance by managing hierarchical information in a separate table. This paper also describes the implementation and evaluation results. This paper also shows the experiment and evaluation result and the comparative analysis on both results. The experiment and evaluation show our proposal provides a prominent performance as against Jena2.

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Enhancement for Magnetic Property of Ba-ferrite for Perpendicular Magnetic Recording Using Ultrasonic Dispersion (초음파 분산에 의한 수직자기기록용 Ba-ferrite의 자기적 특성 향상)

  • Choi, Hyun-Seung;Kim, Chang-Gon;Jang, Hak-Jin;Jung, Ji-Hyung;Yoon, Seog-Young;Kim, Tae-Ok
    • Journal of the Korean Ceramic Society
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    • v.39 no.8
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    • pp.758-763
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    • 2002
  • The various ultrasonic energies (28 kHz, 40 kHz, 70 kHz) were used to improve the magnetic properties of Ba-ferrite as the perpendicular magnetic recording materials. In the sheet formation process, the different orientation hars were used to orientate perpendicularly the dispersed Ba-ferrite to sheet. Throughout these experiments, we have obtained relatively higher value of S. Q. (Squreness Ratio : 0.783) and O. R. (Orientation Ratio : 2.87) magnetic properties at 2 h ultrasonic treatment of 40 kHz ultrasonic energy. With aid of SEM(Scanning Electron Microscopy) images, the obtained sheet with dispersed of Ba-ferrite could be used for perpendicular magnetic recording due to orientated to easy magnetization axis, c-axis. In addition, the value of S. Q. of sheet decreased with increasing applied magnetic field angle during measuring of S. Q. value with changing applied magnetic field angle by VSM (Vibrating Sample Magnetrometer). This result also induced the probability for prependicular magnetic recording.

Design of Summer Very Short-term Precipitation Forecasting Pattern in Metropolitan Area Using Optimized RBFNNs (최적화된 다항식 방사형 기저함수 신경회로망을 이용한 수도권 여름철 초단기 강수예측 패턴 설계)

  • Kim, Hyun-Ki;Choi, Woo-Yong;Oh, Sung-Kwun
    • Journal of the Korean Institute of Intelligent Systems
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    • v.23 no.6
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    • pp.533-538
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    • 2013
  • The damage caused by Recent frequently occurring locality torrential rains is increasing rapidly. In case of densely populated metropolitan area, casualties and property damage is a serious due to landslides and debris flows and floods. Therefore, the importance of predictions about the torrential is increasing. Precipitation characteristic of the bad weather in Korea is divided into typhoons and torrential rains. This seems to vary depending on the duration and area. Rainfall is difficult to predict because regional precipitation is large volatility and nonlinear. In this paper, Very short-term precipitation forecasting pattern model is implemented using KLAPS data used by Korea Meteorological Administration. we designed very short term precipitation forecasting pattern model using GA-based RBFNNs. the structural and parametric values such as the number of Inputs, polynomial type,number of fcm cluster, and fuzzification coefficient are optimized by GA optimization algorithm.

Effect of Annealing on Structural and Electrical Properties of VOx Thin Films (VOx 박막의 구조적 특성과 전기적 특성에 대한 열처리 영향)

  • Lee, Jang Woo;Chung, Chee Won
    • Applied Chemistry for Engineering
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    • v.17 no.5
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    • pp.471-475
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    • 2006
  • $VO_x$ thin films with the thickness of 450 nm were prepared on a $Pt/Ti/SiO_{2}/Si$ substrate at room temperature by a reactive radio frequency (rf) magnetron sputtering method. The deposition rates of $VO_x$ thin films were investigated as a function of $O_{2}$ concentration and rf power. As the $O_{2}$ concentration in a $O_{2}/Ar$ mixture increased, the deposition rate decreased. However, the deposition rate increased with increasing rf power. The deposited $VO_x$ thin films were annealed at $450^{\circ}C$ for 2, 4, and 6 h in $O_{2}$ and $N_{2}$ ambient. After annealing, the phase changes of $VO_x$ thin films were investigated using X-ray diffraction analysis. The plane and cross-sectional views of $VO_x$ thin films before and after annealing were observed by field emission scanning electron microscopy. The metal-insulator transition (MIT) properties of $VO_x$ thin films were measured using current-voltage measurement. The excellent MIT properties were observed in $VO_x$ thin films annealed in $O_{2}$ ambient.

Recovery of Protein Hydrolysate from Hoki (Johnius belengeri) Frame with Tuna Pyloric Caeca Crude Enzyme and Its Functionalities (참치 유래 조효소를 이용한 민태(Johnius belengeri) Frame으로부터 단백질 가수분해물의 회수 및 그 기능성)

  • Jeon, You-Jin;Lee, Byoung-Jo;Byun, Hee-Guk;Kim, Jong-Bae;Kim, Se-Kwon
    • Applied Biological Chemistry
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    • v.42 no.1
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    • pp.49-57
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    • 1999
  • Enzymatic hydrolysis with tuna pyloric caeca crude enzyme(TPCCE) was performed to recover a protein hydrolysate from hoki frame, fish processing by-product. Optimum hydrolytic conditions were pH 10.0, temperature $50^{\circ}C$, and incubation time 12 hrs, and then the degree of hydrolysis was about 60%. The yield of the hydrolysate from hoki frame by enzymatic hydrolysis was approximately 77% on a dry weight basis. The prepared protein hydrolysates were also fractionated through a series of 30, 10, 5 and 1 kDa molecular weight cut-off (MWCO) membranes in order to investigate the effect of their functionalities according to the difference of their molecular size. As the result of studying functionalities of the hydrolysates, 1 K hydrolysate showed the highest solubility over all pHs, and 30 and 10 K hydrolysate showed more excellent emulsifying property and whippability than the other hydrolysates.

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Property Changes of Mung Bean Depending on Hydrolysis Conditions (녹두의 가수분해조건에 따른 특성 변화)

  • Gu, Young-Ah;Jang, Se-Young;Park, Nan-Young;Mun, Chae-Ryun;Kim, Ok-Mi;Jeong, Yong-Jin
    • Food Science and Preservation
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    • v.13 no.5
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    • pp.563-568
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    • 2006
  • The properties of mung bean were investigated depending on hydrolysis condition. The results showed that enzyme treatments (${\alpha}$-amylase and protease each at 0.1% (w/w)) by varying hydrolysis temperature showed better properties than non-pretense treatment (control group). The treatment with 0.08% ${\alpha}$-amylase was best for optimum hydrolysis of mung bean starch The treatment using a mixture of 0.08% (w/w) ${\alpha}$-amylase and 0.12% (w/w) protease was best for optimum hydrolysis of meg bean protein. The effects of Hydrolysis time of mung bean showed that the optimum time was 60 and 90 min and there fore the optimum time was set at 60 min. These result showed that the best hydrolysis conditions of mung bean were the treatment at $60^{\circ}C$ for 60 min using a mixture of 0.08%(w/w) ${\alpha}$-amylase and 0.12% (w/w) pretense, with the sugar level shown at $5.8^{\circ}Brix$, reducing sugar at 2,022.13 mg% and crude protein at 7,666.17 mg%.

Effect of Priming Treatment Using PEG on Seed Germination in Acer mono Max. (PEG를 이용한 priming 처리가 고로쇠나무 종자 발아에 미치는 영향)

  • Tak, Woo-Sik;Cho, Kyeong-Jin;Choi, Chung-Ho
    • Korean Journal of Plant Resources
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    • v.19 no.4
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    • pp.480-487
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    • 2006
  • This study was conducted to improve the germination percent and germination speed by seed priming using polyethylene glycol (PEG) 8,000 solution on Acer mono seed because it has low and irregular germination characteristics. Seed priming was carried out under 0, -0.25, -0.5, -1.0 and -2.0 MPa of water potential at $20^{\circ}C$ for 3 days. Primed seeds showed higher percent germination, germination speed, mean germination time (MGT) and germination performance index (GPI) than unprimed seeds. Especially, primed seed with -2.0 MPa of water potential had the highest germination properties. And primed seeds had a lower dormancy than the unprimed seeds. Relative growth rate and T/R ratio of seedling from primed seeds were measured to survey the effect of seed priming on the seedling vigor as well as seed germination. The seedlings from primed seeds at -2.0 MPa of water potential showed the highest relative growth rates of height and root collar diameter, however, the lowest value in T/R ratio. This study suggested that seeds priming at -2.0 MPa of water potential under $20^{\circ}C$ for 3 days enhanced not only the percent germination, germination speed, MGT and GPI but also the vigor and growth of seedlings in Acer mono.

Fabrication of Gd2O2S:Tb fine scintillator film and evaluation of image quality for resolution improvement of X-ray imaging based on CMOS (CMOS 기반 X선 영상의 해상력 향상을 위한 Gd2O2S:Tb 미세형광체 필름 제작 및 영상 질 평가)

  • Kang, Sang-Sik;Choi, Young-Zoon;Jung, Bong-Jae;No, Si-Cheul;Cho, Chan-Hoon;Yoon, In-Chan;Park, Ji-Koon
    • Journal of the Korean Society of Radiology
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    • v.5 no.5
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    • pp.283-287
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    • 2011
  • In this study, fine $Gd_2O_2S$:Tb powder was synthesized by using a low temperature solution-combustion method for a high-resolution digital x-ray imaging detector. From the fabricated phosphor power, the fine scintillator films was fabricated by particle sedimentation method and was investigated the luminescent property. From the experimental results of relative light output as a function of terbium concentration, the highest luminescent efficiency has at 5 wt% Tb concentration, and luminescent intensity decreased rapidly according to quenching effect about higher Tb concentration. Also, the relative light output of $270{\mu}m$-$Gd_2O_2S$:Tb film has 2945 pC/$cm^2$/mR. And light intensity was saturated at higher film thickness. Finally, to evaluate an image acquisition performance of fabricated phosphor, images were obtained by using commercial CMOS sensor and measured the MTF, NPS, and DQE. DQE(0 lp/mm) of fine phosphor film has 37%. But, DQE improvement of fine phosphor film is possible by resolving problem of film fabrication process and has a significant potential in the application of digital radiation imaging system later.

Residual Stress Behavior of PMDA/6FDA-PDA Copolyimide Thin Films (PMDA/6FDA-PDA 공중합 폴리이미드의 잔류응력 거동)

  • Jang, Won Bong;Chung, Hyun Soo;Joe, Yungil;Han, Haksoo
    • Applied Chemistry for Engineering
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    • v.10 no.7
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    • pp.1014-1019
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    • 1999
  • Copolyamic acid PMDA/6FDA-PDA(PAA) and homopolyamic acids PMDA-PDA(PAA) and 6FDA-PDA(PAA) were synthesized from 1,2,4,5-benzenetetracarboxylic dianhydride(PMDA) and 2,2'-bis(3,4-dicarboxyphenyl) hexafluoropropane dianhydride(6FDA) as the dianhydride and 1,4-phenylenediamine (PDA) as the diamine. Residual stresses were detected in-situ during thermal imidization of the co- and homopolyimide precursors as a function of processing temperature over the range of $25{\sim}400^{\circ}C$ using thin film stress analyzer(TFSA), and morphological structures were investigated by WAXD. In comparison, the resultant residual stress of polyimide films composed of different compositions decreased with the increasing content of PMDA unit in the chain and was about 5 Mpa in compression mode for PMDA-PDA. In this study, the synthesis of random PMDA/6FDA-PDA copolyimide could be completed and compensate for the difficulty of process due to high $T_g$ of PMDA-PDA and relatively higher stress of 6FDA-PDA. It showed that we can make a low level stress copolyimied having excellent mechanical properties by incorporating appropriate rod-like rigid structure PMDA-PDA unit into 6FDA-PDA polyimide backbone which generally shows higher stress due to rotational hinges such as bulky di(trifluoromethyl). Specially, PMDA/6FDA-PDA(0.9:0.1:1.0) satisfied excellent mechanical property and low level stress as an inter layer showing low dielectric constant.

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