• Textile Coloration and Finishing
• /
• v.24 no.3
• /
• pp.196-203
• /
• 2012

The purpose of this study was to investigate usefulness of the pine needles extract on Atopic Dermatitis(AD)-like skin lesions. To investigate the effect of pine needles extract in vivo, atopic dermatitis (NC/Nga) mice using DNCB (2.4-Dinitrochlorbenzene) was used. NC/Nga mice were challenged with DNCB during 2 weeks to develope AD-like skin lesion. After that, pine needles extract was applied to AD-like skin lesion on the backs of the NC/Nga mice during 3 weeks. The efficacy of pine needles extract in the NC/Nga mice was evaluated by measurement of the skin lesion severity(NC mouse score), the serum IgE level, epidermal thickness changes, and mast cell number. Blood was collected from the retro-orbital area and the level of IgE in the blood was measured. The epidermal thickness and mast cell number were observed by microscopic method after H&E stain. The serum IgE levels were decreased after treatment with pine needles extract. The epidermal thickness and mast cell number were decreased after treatment with pine needles extract. To conclude, the topical application of pine needles extract suppressed the progression of AD-like skin lesion.

• The Korean Journal of Ecology
• /
• v.20 no.5
• /
• pp.315-321
• /
• 1997

Peroxidase activities and isozyme patte군 of the pine needles (Pinus densiflora) were examined and compared in the coastal regions of Anmyum-Do(Choongnam, Taean-Gun) and inland regions of Shinchang-Myun(Choongnam, Asan-City). The pine needle peroxidase from Anmyum-Do showed approximately three times higher specfic activity than Shinchang pine needle peroxidase. The pine needle extracts of Anmyun-Do and Shinchang contained three anionic isoperoxidases, named A1, A2 and A3, when subjected to starch gel electrophoresis at pH 7.0. Cjationic isoperoxidases could not be found in both extracts., However, there existed unique isoperoxidase An only from the extracts of Anmyun-Do pine needles under the salinary environment. Moreover, the specific activities of catalase and glucose-6-phosphate dehydrogenase from Anmyun-Do, known for the inducible enzymes under the stress condition, were about 1.8 times higher than those of Shinchang pine needles. However, the specific activities of other enzymes did not show great differences between the two regions. Considering the above results of the higher specific activity of peroxidase and the unique expression of isoperoxidase An, pine needle peroxidase might involve in the defence mechanism against the salinary stress of Anmyun-Do.

• Journal of Forest and Environmental Science
• /
• v.33 no.2
• /
• pp.105-112
• /
• 2017

The distribution and form of pine wood nematode (PWN) were investigated in Jinju-si and Sacheon-si areas, where infested with pine wilt disease (PWD). The average PWN population per g of the tubulation part in the wilted Japanese balck pine were 381.2 and 341 in Jinju-si and Sacheon-si, respectively. The PWN population per g of affected Japanese black pine with wilt rate below 60% were 556 and 518 in Jinju-si and Sacheon-si, respectively. The ratios of PWN and other plant-parasitic nematode in the wilted Japanse black pines were 48% vs. 52% in Jinju-si and 53% vs. 47% in Sacheon-si after exposure to PWD for 1 year. The survivorship of pine wood nematode in the sampled trees after 1 year was 0-10% in the Jinju-si area and 5-20% detected in the Sacheon-si area. In the forest areas affected by PWN, the highest survival rates were 37.2% and 39.8% at 25 cm diameter at breast height (DBH) in Jinju-si and Sacehon-si, respectively, while the highest wilt rates were 30.5% and 28.3% at 30 cm DBH in Jinju-si and Sacehon-si, respectively.

• Journal of Ecology and Environment
• /
• v.32 no.2
• /
• pp.97-102
• /
• 2009

This study was carried out to evaluate litterfall dynamics in three adjacent coniferous tree plantations (larch: Larix leptolepis; red pine: Pinus densiflora; rigitaeda pine: P. rigitaeda) planted in the same year (1963), and growing under similar environmental conditions in the Sambong Exhibition Forests, Hamyang-gun, Gyeongsangnam-do. Litter was collected monthly between July 2006 and June 2008. Needle, broad leaf and total litter inputs followed a similar monthly pattern in the three coniferous plantations. The amounts of needles, flowers, and miscellaneous litter were significantly lower in the larch than in the two pine plantations, while branch litter was significantly higher in the larch than in the two pine plantations. Average total litterfall for two years was significantly higher for the pine (5,475 kg $ha^{-1}\;yr^{-1}$ for red pine and 5,290 kg $ha^{-1}\;yr^{-1}$ for rigitaeda pine) plantations than for the larch (3,953 kg $ha^{-1}\;yr^{-1}$ plantation. Needle litter comprised about 73.1% of total litterfall for the rigitaeda pine, 70.8% for the red pine and 62.9% for the larch plantations. Our results demonstrate that litterfall inputs can be affected by tree species.

• Korean journal of food and cookery science
• /
• v.33 no.2
• /
• pp.174-180
• /
• 2017

Purpose: The purpose of this study was to investigate quality characteristics and antioxidant effects on storage by temperature between new cultivar Dewdrop Pine Mushroom (Lentinula edodes GNA01) and button mushroom (Agaricus bisporus). Methods: Dewdrop Pine Mushroom and button mushroom were prepared at low storage temperature. Results: Weight loss increased with time, whereas the new cultivar Dewdrop Pine Mushroom showed a decrease of less than 4% until 15days. Hardness of new cultivar Dewdrop Pine Mushroom was reduces less than button mushroom during storage. Color change of new cultivar Dewdrop Pine Mushroom was not altered during storage. For viable cell count, new cultivar Dewdrop Pine Mushroom proliferated less than button mushroom. For antioxidant activity, polyphenol content increased with storage period of both mushrooms. The electron-donating action of new cultivar Dewdrop Pine Mushroom maintained high antioxidant activity accounting for 80% until 12 days of storage. New cultivar Dewdrop Pine Mushroom was evaluated as better than pine mushroom as it exceeded the middle of storage in sensory characteristics; such as appearance, color, aroma and overall acceptability, etc. Conclusion: In summary, new cultivar Dewdrop Pine Mushroom was stored for 12 days while button mushroom was stored for 9 days.

• Journal of Environmental Science International
• /
• v.19 no.3
• /
• pp.305-312
• /
• 2010

The purpose of this study is to compare the relative growth of annual ring width of red pine(Pinus densiflora), black pine(Pinus thunbergii) and pitch pine(Pinus rigida) by means of multiple regression method according to Graybill hypothesis. The obtained results are as follows. 1. The changes of rainfall have affected to tree growth during the periods of 1975 through 1978. 2. Among these pine trees, red pine was mostly influenced by environmental factors. 3. The growth of annual ring width was sensitively responded to the changes of rainfall and air temperature. 4. Among the heavy metals analyzed, the concentrations(ppm) of Lead(Pb) and Copper(Cu) were negatively effected on the growth of annual ring width of pine trees. 5. The analytical technique of annual ring width may be useful for estimation of the pollution in forest areas near industrial complexes.

• Journal of the Korean Applied Science and Technology
• /
• v.22 no.1
• /
• pp.71-76
• /
• 2005

We extracted pine-needles using ethanol as solvent, and we obtained the refined oil component from pine-needles extract. Also we tested the tyrosinase activated inhibition effect with melanin experiment and analysed with ICP/OES and UV/VIS. Accordingly we obtained the next conclusion from the result of this experiment. From the first result of this experiment, we could know that the degree of recovery of refined oil component from pine-needles extract appeared in about 8.0%. From the second result of this experiment, we could know that the tyrosinase activated inhibition rate increased more and more in case of increasing concentration of pine-needles, green-tea, vitamine-C. Also we could know that vitarnine-C influences to tyrosinase activated inhibition contained in pine-needles. From the third result of this experiment, we could know that inorganic materials of Ca, Mg, V, Mn, etc contained in pine-needles detected with ICP/OES analysis, and the absorbance of pine-needles extract appeared very high in UV/VIS analysis.

• Food Science and Preservation
• /
• v.6 no.1
• /
• pp.115-120
• /
• 1999

This study was carried out to investigate the effect of Pine needle extract on lipid oxidation and free radical reaction in iron sources reacted with active oxygen species. The results were summarized as follow; The pine needle extracts didn`t show a distinct effect on reduction of lipid oxidation if the iron ion didn`t exist in oil emulsion. The pine needle extracts played role as a strong chelating agents to bind iron ion if Ferrous iron(Fe\ulcorner) exist in oil emulsion. Ferric iron(Fe) was lower effect than Ferrous iron(Fe) on free radical reaction in oil emulsion. And also, the Fe\ulcorner reacted with pine needle extract did not show distinct effect on free radical reaction, compared to Fe\ulcorner reacted with pine needle extract. And also, Pine needle extracts reacted with H\ulcornerO\ulcorner were tended to show a low oxygen scavenging ability in case of H\ulcornerO\ulcorner only was existed, compared to those of H\ulcornerO\ulcorner + Fe\ulcorner complex. Pine needle extracts were the most powerful Fe\ulcorner binding agents, compared to other strong synthetic antioxidants such as EDTA and DTPA.

• Journal of Korean Society for Atmospheric Environment
• /
• v.18 no.6
• /
• pp.515-525
• /
• 2002

A field study was conducted to estimate the emission rate of biogenic volatile organic compounds (BVOCs) from pine trees. In addition, the influences of meteological variables on their distribution characteristics have been investigated. A vegetation enclosure chamber was designed and constructed of Tedlar bag and acril. Sorbent tubes made up of Tenax TA and Carbotrap were used to collect biogenic VOCs emitted from each individual tree. Analysis of BVOCs was performed using a GC-FID system. The fundamental analytical parameters including linearity, retention time, recovery efficiency, and breakthrough volume were examined and verified for the determination of monoterpene emission rates. Total average concentration of each component is found to be $\alpha$-pinene (16.5), $\beta$-pinene (4.61) from pine trees, and $\alpha$-pinene (42.4), $\beta$-pinene (18.7 ng(gdw)$^{-1}$ hr$^{-1}$ ) from Korean pine trees. On the basis of our study, $\alpha$-pinene was found to be the major monoterpene emitted from both pine and Korean pine trees which were accompanied by $\beta$-pinene, camphene, and limonene. In ambient air, variable monoterpene compositions of emissions from pine trees were similar to Korean pine trees. Emission rates of monoterpene from each tree were found to depend on such parameters as temperature and solar radiation.

• Journal of Environmental Health Sciences
• /
• v.32 no.2 s.89
• /
• pp.111-117
• /
• 2006

Polybrominated diphenyl ethers (PBDEs) in pine needles and soil samples from rural area were analyzed in this study. Concentrations of PBDE congeners were ranged between 0.2 and 3104 pg/g DW(dry weight) in pine needles and between 2.4 and 1997 pg/g DW in soil samples. The most abundant congener both in pine needles and soil samples was BDE-209, which was related to importation and production of deca-BDE technical mixtures in Korean industry. And BDE-99, BDE-47, BDE-100 deposited in pine needles and soil samples were closely matched with the major constituents of the penta-BDE technical mixture. Those can be interpreted as an evidence that transfer of the congeners in deca-BDE and penta-BDE technical mixtures from source to pine needle and soil occurs with broadly similar efficiency. Correlation coefficient(r) of PBDE congeners deposited in pine needles and soil samples is very significant because it implies th at there has been little weathering/degradation/alteration of the congeners generated from sources during atmospheric transport or within pine needles and soil themselves.