• Journal of Periodontal and Implant Science
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• v.33 no.2
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• pp.193-213
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• 2003

The purpose of this study is to evaluate the phenomenon of attachment and spreading of the cultured rat　calvarial cell inoculated on their surface of different kinds of biodegradable membrane which had been used on tissue regeneration on periodontal defects by using scanning electron microscope. In this experiment 30 Sprague-Dawley male rats (mean BW 150gm) were used to harvest abundant number of cell in the short period. The rats were sacrificed by decapitatioan to obtain the calvaria for bone cell culture. Calvarial cells were cultured with Dulbecco's Modified Essential Medium contained with 10% Fetal Bovine Serum under the conventional conditions. Biodegradable barrier membrane were collected with collagen type, and were divided into 3 different kind of surface such as scattered, polarized and fine-net type as their surface texture. Microcover plate which usually used for cell culture was used as control for smooth surface. All the membrane were seeded with cultured calvarial cell on their surface. The number of cell inoculated on the membrane were $1{\times}10^6$ Cells/ml. After the culture as designed time, all the membrane were washed with 0.1 M Phosphate Buffered saline and fuxed with 2.5% Glutaraldehyde. And all specimen were treated with $OsO_4$, and Tannic acid before drying the cell for coating the cell with gold. Scanning Electron Microscope was used to observation. The following results were obtained. I. During the whole period of experiment, the phenomenon of cell attachment and spreading were revealed similar pattern to compare with smooth surface culture plate and ordinary culture dish. 2. The shape of cell attachment and spreading on the surface of barrier membrane were observed no remarked difference pattern between smooth surface culture plate and ordinary culture dish. 3. The cytoplasmic process of cultured calvaria cell extent to the deep portion of barrier membrane like as their own proper shape. 4. There were no remarkable relationships between the degree of cultured cell spreading and surface structure of barrier membrane. 5. Slight starified layer of cultured calvaria cell were observed on the scattered type of resorbable membrane, Conclusively, this study thus suggest that cultured bone cell inoculated onto the biodegradable barrier membrane may have an important role of carrier for many cell which could be used as new tissue regeneration, and those tissue engeering technique may become an new method in the approach to the repair of bone defects.

• The Korean Journal of Pesticide Science
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• v.3 no.1
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• pp.66-77
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• 1999

In order to elucidate the fate of the residues of the bipyridylium herbicide paraquat in soil, maize plants were grown for 4 weeks on the specially-made pots filled with two different types of soils containing fresh and 6-week-aged residues of [$^{14}C$]paraquat, respectively. The mineralization of [$^{14}C$]paraquat to $^{14}CO_{2}$ during the aging period and the cultivation period of maize plants amounted to $0.13{\sim}0.18%$ and $0.02{\sim}0.17%$, respectively, of the original $^{14}C$ activities. At harvest the roots and shoots contained less than 0.1% and 0.01% of the originally applied $^{14}C$ activities, respectively, whereas the $^{14}C$ activities remaining in soil were more than 97% in both soils. The water extractability of the soil where maize plants were grown for 4 weeks was less than 1.2% of the original $^{14}C$ activities. Most of the non-extractable soil-bound residues of [$^{14}C$]paraquat were incorporated into the humin fraction. Soil pHs during the aging of soil B and after cultivation in all treatments increased. The distribution of the $^{14}C$ activities in subcellular particles of the maize plant roots was the highest in the residue fraction(incompletely homogenized tissue). Dehydrogenase activities increased after vegetation, regardless of soil aging.

• The Korean Journal of Mycology
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• v.47 no.4
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• pp.385-392
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• 2019

Currently, plastic bags are being used for sawdust cultivation of shiitake mushroom. However, due to serious environmental problems caused by the use of plastic bags, we studied the efficacy of bottle cultivation method to replace the sawdust bag method. Small detachable plastic bottles (400 g capacity) filled with Quercus spp. sawdust and wheat bran (4:1 w/w) media were incubated for 80 and 120 days. The weight loss (%) of the media was higher for the NIFoS 2464 strain at an approximate light intensity of 300 Lux than light intensity of 500 Lux; the light intensity was associated with the loss of sawdust medium-weight during the cultivation period. The highest yield was observed when the strain was cultivated for 80 days under dark conditions, 40 days under 500 Lux light, and air circulation fan speed of 30 rpm. When incubated for 120 days, mushroom yield in the bottle media was higher at 40 days of light exposure than 20 days of light exposure. In the bottle media incubated for 80 days under dark conditions, the mushrooms fruited due to repetitive water spraying on the top of the media and light stimulation during the fruiting period. The media could be separated from the bottles because the media shrank after the first harvest. These separated plastic bottles could be re-used for mushroom cultivation, thereby reducing the amount of plastic waste.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.198-198
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• 2017

Cultivated varieties of sweet potato were from dry texture type to tender texture type on the basis of consumer preferences. There are many differences in the quantity of sweet potato, starch content, pigment, and sugar content depending on the cultivation season and area, even in the same variety. Therefore, in this study, we attempted to establish optimum time of harvesting through growth characteristics and variation in component like starch, sugar, polyphenol and flavonoid. Four sweet potato varieties were used in this experiment. Among them, Jinhongmi (JHM) & Yulmi (YM) were as dry texture type and Pungwonmi (PWM) & Hogammi (HGM) were as tender texture type. Sweet potatoes were transplanted on 23 May, 2016 and were investigated storage root weight and component contents every 20 days from 60 days to 120 days and surveyed yield at 110, 120, 130 days after transplantation. Result revealed that storage root weight of YM, JHM, and HGM were 30.1, 38.9, 20.8 g respectively in 60 days after transplanting. Storage roots of PWM gerw faster with the weight of 88.2 g. In 120 days after transplanting, storage root weight varied from 88.3 to 118.7 g, HGM was the smallest, and PWM was the largest. Sugar contents of sweet potato ranged from 21.0 to $23.8Brix^{\circ}$ in 60 days after transplanting and from 27.5 to $30.78Brix^{\circ}$ in 120 days after transplanting. In particular, the sugar content of HGM was the highest over $30Brix^{\circ}$ after 80 days. The starch content of dry texture type (YM, JHM) increased from 15.5% to 20.4% and tender texture type (PWM, HGM) increased from 11.0% to 17.3%. Starch content tended to be high in dry type sweet potatoes. The content of polyphenol and flavonoid were highest in 60 days after transplanting and was reduced according to cultivation period. The total yield of PWM was high as 3,154 kg/10a and large storage root of over 250 g accounted for 47.4% in 110 days after transplanting. Storage root (YM, JHM, HGM) of 81~150 g accounted for 34.9% ~ 43.2% in 120 days after transplanting. These are the most marketable. Because consumer in Korea prefers small, round and about 100g size sweet potato. The ratio of large storage root (over 250 g) were increased in all varieties at 130 days after transplanting. Therefore, it is considered appropriate to harvest PWM at 110 days and YM, JHM, HGM at 120 days after transplanting, which planted in late May.

• Journal of Mushroom
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• v.19 no.2
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• pp.96-102
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• 2021

A new cultivar of oak mushroom 'Dadam' was bred from monokaryotic strains of 'GMLE36062-4' and 'GMLE36288-34'. The optimum temperature for mycelial growth of the new cultivar 'Dadam' on potato dextrose agar was 19-22℃. Total cultivation period of the new cultivar, from innoculation to its first harvest, was 135-139 days, similar to that of the control cultivar 'Hwadam'. The pileus color and stipe thickness of the new cultivar were darker and thinner than those of 'Hwadam'. Total yield of 'Dadam' was 621 g per 3 kg substrate, and is higher than that of 'Hwadam' (371 g). In farmhouse field test, it showed that the period of mycelial growth, browning, and fruiting body formation were the same as those of the control cultivar L808. The number of available fruiting bodies of 'Dadam' was 15, and is lower than that of the control (47), therefore, it was possible to save workforce in thinning. The total yield for 2 flush was 480 g for 'Dadam', similar to the 473 g of the control cultivar.

• Korean Journal of Organic Agriculture
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• v.31 no.4
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• pp.413-426
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• 2023

These experiments were conducted for 3 years from 2021 to 2023 to develop a method that can be safely used to prevent the leakage of Golden apple snails (Pomacea canaliculata) from eco-friendly rice plantations. In the southern part of Jeollanam-do, after planting rice, the young golden apple snails placed in the rice field become adults around mid-July and begin spawning. These individuals can overwinter in drains that do not dry out, but individuals hatched after mid-July will not mature enough to overwinter. The size of golden apple snails overwintered in the drainage canal was more than 2.5cm in shell height. Installing a net at the inlet could block 95% of the inflow of snails, and 99% of outflow was blocked by installing an improved water trap and net at the drain. During the mid-drying period and pre-harvest drying period, a water path was created with a power paddy pottery machine. 59.5% of snails were attracted to the waterway, and it took 130 minutes to build the waterway and collect the snails. Based on these results, seasonal paddy management tips for the safe use of giant snails in rice fields were suggested.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.20 no.3
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• pp.237-247
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• 1987

The present study deals with the physico-chemical and meteorological conditions in Porphyra-cultivation ground to determine the relationship between laver production and its environmental factors in Kwangyang Bay from January to April in 1986. As a result, major environmental factors which are thought to have a great influence upon the poor harvest during the cultivation period are as follows; 1) the excessive rainfall in the beginning of cultivation period 2) the accumulation of suspended matters on the thallus of laver 3) the decrease of current velocity and the stagnation of the water in the cultivation ground.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.3
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• pp.273-278
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• 2008

This study set out to investigate the changes to the growth, yield, and flavonoid contents of mungbean according to different seeding times from mid May to mid July in the southern region of Korea. Days to first flowering, days to first maturing, and cultivation period were shorter at later seeding time than earlier seeding time. But later the seeding time was, days from first maturing to first harvesting, days required between harvesting increased. Number of pods at first harvesting and yield of mungbean were highest when seeded at late June, showing increase in yield 14% more than at early June as standard seeding time. However number of seeds per pod and 1,000-seed weight at first harvesting were highest when seeded at mid July. The number of harvesting was smallest at two times when seeded at June or mid July. The contents of average vitexin and isovitexin in mungbean were highest in the order of mid July, late June, and mid May. In particular, their contents reached its highest point in the seeds of the second harvest. Considering the results of the cultivation period, yield, harvesting times, vitexin and isovitexin contents, the proper seeding time of mungbeans in the southern region of Korea will be from late June to mid July.

• The Korean Journal of Mycology
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• v.50 no.2
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• pp.93-102
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• 2022

A new cultivar of oak mushroom Lentinula edodes 'Jadam' was bred from monokaryotic strains of 'Hwadam-18' and 'GMLE36295-22'. The optimum temperature for mycelial growth of 'Jadam' on potato dextrose agar was 22-25℃. Total cultivation period of the new cultivar, from inoculation to its first harvest, was 122-124 days, shorter to that of the control cultivar 'Hwadam'. Total yield of 'Jadam' was 623.8 g per 3 kg substrate, and is higher than that of 'Hwadam' (455.2 g). In the fruiting body of the new cultivar, the stipe was shorter and thinner than those of 'Hwadam'. A farmhouse field test showed that the period of mycelial growth and browning was shorter than that of control cultivar 'L808'. The total yield for 2 flush was 543.3 g for 'Jadam'. It was similar to the 585.3 g of the control cultivar. Therefore, the new cultivar 'Jadam' could be a substitute for 'L808' in the field of farms.

• Journal of Mushroom
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• v.10 no.4
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• pp.224-235
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• 2012

Quality change of winter mushroom were investigated during storage and distribution phase as influenced by storage temperature. According to storage period and temperature, amino acids, were analysed and quantified with the mushroom fruiting bodies using HPLC and morphological characteristics were investigated. Characteristic changes of winter mushroom fruiting bodies were described as follows during storage according to the storage temperature. Looking at the results of the analyzed amino acid contents, temperature between $-1^{\circ}C$ and $4^{\circ}C$ was optimal condition for the storage. At $4^{\circ}C$, the chemical composition tended to be maintained. On the other hand, the results indicate the rapid loss of nutrition at $-1^{\circ}C$ within 7 days of storage. Exceptionally, proline was shown to be increased. Brown line mushroom had a larger loss than white line mushroom. Based on this result, brown line mushroom have shown significant differences among varieties. Therefore, winter mushroom should be stored at $4^{\circ}C$ to minimize nutrient loss and to maintain freshness and mushrooms should be consumed within 14 days after harvest.