• 한국발생생물학회지:발생과생식
• /
• 제16권4호
• /
• pp.353-361
• /
• 2012

Human embryonic stem (ES) cells are a potential source of cells for developmental studies and for a variety of applications in transplantation therapies and drug discovery. However, human ES cells are difficult to culture and maintain at a large scale, which is one of the most serious obstacles in human ES cell research. Culture of human ES cells on MEF cells after disassociation with accutase has previously been demonstrated by other research groups. Here, we confirmed that human ES cells (H9) can maintain stem cell properties when the cells are passaged as single cells under a feeder-free culture condition. Accutase-dissociated human ES cells showed normal karyotype, stem cell marker expression, and morphology. We prepared frozen stocks during the culture period, thawed two of the human ES cell stocks, and analyzed the cells after culture with the same method. Although the cells revealed normal expression of stem cell marker genes, they had abnormal karyotypes. Therefore, we suggest that accutase-dissociated single cells can be usefully expanded in a feeder-free condition but chromosomal modification should be considered in the culture after freeze-thawing.

• 한국가축번식학회지
• /
• 제16권2호
• /
• pp.117-123
• /
• 1992

This Study was carried out ot investigate the effects of concentration and equilibration time of cryoprotective aagents on survival rate of slowly and ultrarapidly frozen porcine embryos. The porcine embryos following dehydration by cryoprotective agents and 0.25M sucrose were slowly freezed(from 2$0^{\circ}C$ to -7$^{\circ}C$/-1$^{\circ}C$/min., from -7$^{\circ}C$ to -35$^{\circ}C$/-0.2$^{\circ}C$/min., from -35$^{\circ}C$ to -38$^{\circ}C$/-0.3$^{\circ}C$/min.) by Cell Freezer and directly plunged into liquid nitrogen and thawed in 38$^{\circ}C$ water bath. Survival rate was defined as development rate to the morula and blastocyst stage after in vitro culture or by FDA test. The results are summarized as follows : 1. The survival rates of porcine embryos after slow frozen-thawing in the freezing medium of 0.25M sucrose added 2.0M glycerol, 3.0M DMSO, 2.0M propanediol or 2.0M glycerol＋2.0M propanediol was 80.6, 84.7, 75.0 or 78.8%, respectively. 2. The survival rates of porcine embryos after slow frozen-thawing in the freezing medium of 0.50M sucrose added 2.0M glycerol, 3.0M DMSO, 2.0M propanediol or 2.0M glycerol＋2.0M propanediol was 80.9, 82.4, 73.1 or 77.1%, respectively. 3. The survival rates of porcine embryos after ultrarapid frozen-thawing in the freezing medium of 0.25M sucroese added 2.0M glycerol, 3.0M DMSO, 2.0M propanediol or 2.0M glycerol＋2.0M propanediol was 65.3, 68.6, 63.2 or 59.9%, respectively. 4. The survival rates of porcine embryos after ultrapid frozen-thawing in the freezing medium of 0.50M sucrose added 2.0M glycerol, 3.0M DMSO, 2.0 propanediol or 2.0M glycerol＋2.0M propanediol was 67.5, 62.9, 56.9, or 62.8%, respectively. 5. The higher survival rate of porcine embryos was attained at the short period ofequilibration time(5min.) in the freezing medium added 0.25M sucrose and 3.0 DMSO compared to those of 10 or 20min. equilibration time in the same condition.

• 한국가축번식학회지
• /
• 제16권2호
• /
• pp.125-131
• /
• 1992

This study was carried out to investigate the effects of concentration and equilibration time of cryoprotective agents on the survival rate of slowly and ultrarapidly frozen porcine embryos. The porcine embryos following dehydration by cryoprotective agents and a various concentration of sucrose were directly plunged into liquid nitrogen and thawed in 38$^{\circ}C$ water bath. Survival rate was defined as development rat to the morula and blastocyst stage after in vitro culture or by FDA test. The results are summarized as follows : 1. The survival rates of porcine embryos after ultrarapid frozen-thawing in the freezing medium of 0.25M sucrose added 2.0, 2.5, 3.0, 3.5, or 4.0M glycerol was 65.3, 61.8, 64.3, 59.4 or 39.4%, respectively. Addition of 0.25M sucrose into the freezing medium containing 2.0M glycerol showed higher survival rate than those of 2.5~4.0M glycerol. 2. The survival rates of porcine embryos after ultraradpid frozen-thawing in the freezing medium of 0.25M sucroese added 2.0, 2.5, 3.0, 3.5 or 4.0M DMSO was 65.6, 67.6, 68.6, 60.6 or 23.6%, respectively. However, addition of 0.25M sucrose into the freezing medium containing 3.0M DMSO showed higher survival rate than those of 2.0, 2.5, 3.5 or 4.0M DMSO. 3. The survival rates of porcine embryos after ultrapid frozen-thawing in the freezing medium of 0.25M sucrose added 2.0, 2.5, 3.0, 3.5 or 4.0M propanediol was 63.2, 60.3, 62.1, 52.3 or 24.3%, respectively. Addition of 0.25M sucroese into the freezing medium containing 2.0M propanediol showed higher survival rate than those of 2.5~4.0M glycerol. 4. The survival rates of porcine embryos after ultrarapid frozen-thawing the freezing medium of 2.0M glycerol added 0.10, 0.25, 0.50 or 0.75M sucrose was 61.8, 70.8, 67.6 or 52.2%, respectively. Addition of 2.0M glycerol into the freezing medium containing 0.25M sucreose showed higher survival rate than that those of 0.10, 0.50 or 0.75M sucrose. 5. The higher suvival rate of porcine embryos were attained at short period of equilibration time 92.5~5min.) in the freezing medium added 0.25M sucreose and 3.0M compared to those of 10 or 20min. equilibration time in the same condition.

• 한국건축시공학회:학술대회논문집
• /
• 한국건축시공학회 2014년도 추계 학술논문 발표대회
• /
• pp.7-8
• /
• 2014

Latex-modified concrete using ready mix concrete (R-LMC) was developed for application of building construction project (specifically, the rooftop of a parking garage unable to use heavy equipments for bridge deck overlay) due to three major outstanding properties of R-LMC; bond strength, resistance of cracks at early age, and resistance of freezing and thawing. However, R-LMC at the placement stage is required to be sufficiently cured because R-LMC is very sensitive to rate of evaporation of surface moisture. This study focused on effects of different curing methods and climate condition on cracks on the surface of hardened R-LMC considering the chart of rate of evaporation of surface moisture from concrete provided by American Concrete Institute in manual for placement of latex modified concrete.

• 대한토목학회논문집
• /
• 제32권6C호
• /
• pp.275-284
• /
• 2012

남극대륙은 바람이 강하며 지구상에서 가장 기온이 낮고 건조한 극한환경 조건으로서 지표면의 암석은 동결-융해에 의해 암석의 역학적 특성이 변해왔다. 극지 암석의 지반공학적 특성을 이해하기 위하여 남극에서 직접 암석을 채취하여 암석의 물리적 역학적 특성을 분석하였다. 서남극 케이프 벅스 및 동남극 테라노바 만의 암석은 국내 편마암이 보이는 대표적인 물성 값의 범위내에 존재하고 있었으며, 케이프 벅스의 노출된 암석이 테라노바 만에 비하여 암반 강도가 큰 것으로 나타났다. 테라노바 만의 지표에 노출된 암석의 평균 흡수율은 0.56%, 평균 P파 속도는 3,717m/s, 일축압축 강도는 109MPa로 측정되었으며, 시추조사로부터 채취한 심도 2.9m 이상의 암석 평균 흡수율은 0.24%, 평균 P파 속도는 4,670m/s, 일축압축 강도는 88MPa로 측정되었다. 테라노바 만의 지표면에 노출된 암석과 심부에서 채취한 암석을 비교한 결과, 노출된 암석은 동결-융해의 영향으로 판단되는 역학적 특성 변화는 나타나지 않았으나 흡수율-P파 속도 변화의 물리적 특성 변화는 잘 나타났다.

• 한국수정란이식학회지
• /
• 제6권1호
• /
• pp.13-24
• /
• 1991

The objective of this experiment was to study some possibilities to simplify freezing, thawing and transfer procedure of one-step straw method comparing with the conventional methods using bovine embryos. The previous work are also designed to investigate the thawing effect by development stage and its quality using the embryos. Results obtained were summarized as follows: 1. A total of 87 embryos from 14 donor cows were frozen-thawed and an average of frozen embryo/donor was 6.2. 2. The survival rates of morula stage(65.4%) were higher than those of blastocyst stage(57.l%) and vice versa in rate of morphological recovery (80% vs 95.4%). However. no significant difference was denoted between them. 3. In difference between the groups of good quality and poor quality. good quality was resulted in a significantly higher embryo survival rate(75%) and recovery rates(95%) than poor quality(P<0.0l). 4. In effects of non-permeable sugar dilution in added to l.0M glycerol. higher survival rates were orderd in sucrose. lactose, raffinose and xylose. But lactose-raffinose, sucrose-trehalose and xylose in added to 2.OM glycerol. 5. The highest survival rates were obtained by direct plunge into the liquid nitrogen with 3.OM concentration both of glycerol and trehalose. 6. The survival rates in vitro condition of one-step and direct plunge methods(75%-87.5%) were significantly higher than those of multiple steps (21.4-52.6%) in in vitro (P<0.0l). However, the results of single-step were critical in comparing to other steps of final pregnant conformation.

• 대한토목학회논문집
• /
• 제33권3호
• /
• pp.909-916
• /
• 2013

저온에서 소성된 산화마그네슘 분말을 치환한 MgO 콘크리트는 장기적인 팽창성을 가진다. 또한 MgO의 수화반응이 느린 속도로 장기재령까지 일어나기 때문에 매스콘크리트의 온도수축을 효과적으로 보상할 수 있다. 따라서 저온 소성한 MgO는 매스콘크리트 구조물인 댐에 주로 적용되었다. 최근 수화열 저감을 위해 매스콘크리트에 많이 사용되는 플라이애시를 사용한 MgO 콘크리트의 팽창특성에 대한 연구가 진행되어 왔지만 이러한 콘크리트의 내구성에 관한 연구는 부족한 실정이다. 이 연구에서는 플라이애시 콘크리트에 저온 소성한 MgO 분말을 치환하여 MgO 분말 치환에 따른 장기재령에서의 내구특성을 확인하였다. 재령 360일까지 20, $50^{\circ}C$에서 수중 양생을 실시한 후 탄산화, 동결융해 및 염화물 확산, 황산염 침투 저항성을 평가하였다. 실험결과, MgO 분말을 치환한 시편에서 탄산화 저항성 및 염해 저항성, 황산염 침투 저항성이 다소 향상되는 것을 확인하였다. 반면 동결융해 저항성은 MgO 분말 치환에 거의 영향을 받지 않았다.

• 한국구조물진단유지관리공학회 논문집
• /
• 제11권6호
• /
• pp.143-151
• /
• 2007

본 연구에서는 염화물이 함유된 동결수에 의한 콘크리트의 내동해성을 검토하기 위하여 동결융해 및 표면스케일링 저항성을 평가하고자 하였으며, 이를 위한 배합으로서 물결합재비는 0.37, 0.42, 0.47의 3수준, 결합재 방식은 일반 OPC 콘크리트, 고로슬래그 미분말 50%의 2성분계 콘크리트 및 플라이애시 15%와 고로슬래그 미분말 35%의 3성분계 콘크리트로 설정하였다. 그 결과, 고로슬래그 미분말 50% 및 플라이애시 15%와 고로슬래그 미분말 35%의 혼합 시멘트계 콘크리트의 경우 일반 OPC 콘크리트에 비하여 동결융해 및 표면스케일링 저항성이 상대적으로 우수하게 나타났으며, 이를 통해 내구성 저하가 우려되는 해양 환경 하에서 비래염분 및 비말 등의 해수의 작용에 의한 콘크리트의 내구성 저하현상을 억제하기 위한 방안으로서 슬래그의 활용이 유효함을 확인할 수 있었다.

• 한국수정란이식학회지
• /
• 제27권1호
• /
• pp.45-50
• /
• 2012

The objective of this study is to estimate the effect of adding TES to LEY and FGE freezing extender for the sperm viability, acrosomal morphology and DNA fragmentation from miniature pig sperm, we evaluated sperm characteristics in TFGE, TLE and LEY with various thawing condition ($37^{\circ}C$ for 20 sec, 45 sec and $75^{\circ}C$ for 5 sec, respectively), and in different concentration of glycerol at 1%, 1.5%, 3%. The sperm viability and normal acrosome intact(NAI) in TFGE (Viability : $60.3{\pm}2.4$, NAI : $58.6{\pm}2.2%$), TLE ($61.3{\pm}2.4$, $62.2{\pm}2.2%$) extender significantly(p<0.05) increased than that in LEY ($50.2{\pm}2.4$, $54.5{\pm}2.2%$) extender thawed at $75^{\circ}C$ for 5 sec. According to the results from glycerol concentration, the viability and NAI of miniature pig sperm in 1.5% glycerol TLE ($66.1{\pm}3.2$, $66.2{\pm}1.0%$) was highest among the experimental groups. In accordance with this, DNA fragmentation rates was the lowest in TLE ($43.3{\pm}0.5%$) while that in LEY ($63.5{\pm}2.3%$) is the highest. Therefore, these results suggest that TLE extender method for freezing- thawing of miniature pig sperm increased the viability after thawing.

• 한국콘크리트학회:학술대회논문집
• /
• 한국콘크리트학회 1991년도 가을 학술발표회 논문집
• /
• pp.5-10
• /
• 1991

This study was performed to find out the effect about the spacing factor and durability factor to evaluate the durability of concrete in high-strength concrete with freezing and thawing as following each condition, 1) unit cement content : 500kg/$\textrm{m}^3$, 550kg/$\textrm{m}^3$ 2) water/cement ratio : 25%, 30%, 35% 3) air content : below 1.5%, 1.6~3.5%, 4~6%, over 7% From the results tested, a variation of air content was more effective to the durability of concrete than that of water/cement ratio and unit cement content.