• Proceedings of the Korean Geotechical Society Conference
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• 2000.11a
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• pp.543-550
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• 2000

Rock slopes along the road or railroad are affected by temperature and therefore experienced iterative freezing-thawing process between winter and early spring. The purpose of this study is to analyze the stability of rock slopes which are influenced by the deterioration due to the freezing-thawing. The analysis is the homogenization method which evaluates the strength property of discontinuous rock mass, and as a strength failure criterion, Drucker-Prager failure criterion is used. The deterioration property of real rock is obtained by a freezing-thawing laboratory test of tuff and this property of deterioration is quantitated and used as a basic data of stability analysis for rock mass. To evaluate the deterioration depth due to the freezing-thawing in situ rock slope, one dimensional heat conductivity equation is used and as the result I can find that the depth of which is affected by a temperature. After the freezing-thawing depth of model slope is determined, we analyze the pattern of rock mass stength value of rock slope model which excesses the limit of self-load.

• Journal of Food Hygiene and Safety
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• v.3 no.1
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• pp.19-26
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• 1988

Most of meat spoilage bacteria area Gram negative, which are very sensitive to freezing ; for instance , 90% of E. coli cells are killed or sub-lethally injured by freezing at -3$0^{\circ}C$, and the freeze-injury rate is dependent upon freezing rate. Since the injured bacterial cells are sensitive to selective agents, they fail to multiply in selective media. Injured bacterial cells are, however, capable of spontaneous repair at appropriate environmental and nutritional conditions . Enumeration of injured bacterial cells involves artificial induction of repair at these conditions. Cubic beef samples(3$\times$3$\times$3cm) were frozen at -6$0^{\circ}C$, -4$0^{\circ}C$, or -18$^{\circ}C$. The samples frozen at each temperature were thawed at 4$^{\circ}C$, 2$0^{\circ}C$, or by microwave . After these respective freezing an thawing treatments, the percentage of sub-lethally injured total coliforms out of total surviving ones was measured and compared. The results were as follows: 1. The interaction between freezing and thawing on injury rate was not significant. 2. The injury rates(as means of all three thawing treatments post-freezing) by freezing at -6$0^{\circ}C$, -4$0^{\circ}C$, or -18$^{\circ}C$ were 32.2$^{\circ}C$ and 19.2$^{\circ}C$ respectively . 3. The injury rates(as means of all three freezing treatments)by thawing at 4$^{\circ}C$, 2$0^{\circ}C$, or by microwave were 49.3%, 11.7% and 21.0% respectively. The highest injury rate was caused by freezing at -6$0^{\circ}C$ and subsequent thawing at 4$^{\circ}C$. However since the injury rates by freezing treatment were not significantly different, freezing at -18$^{\circ}C$ and subsequent thawing at 4$^{\circ}C$ can also be recommended , from an economic perspective.

• Proceedings of the Korea Concrete Institute Conference
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• 1994.10a
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• pp.205-210
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• 1994

This study was conducted to evaluate performance of asphalt concretes under various densities, using Marshall specimens before and after freezing-and-thawing treatment. Six different compaction blows per side (20, 30, 40, 50, 60, 70 blows) were applied to specimens to produce different densities. Test results showed that the lower density specimens had the weaker resistance to freezing-and-thawing treatment. The density was an index of retaining fatigue life and displacement after freezing-and-thawing. Therefore, poor compaction in pavement was considered to be a major cause of early distress mechanisms such as rutting, ravelling and cracking, which were resulted in a reduced service life.

• Korean Journal of Animal Reproduction
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• v.11 no.2
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• pp.122-126
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• 1987

This study was carried out to investigate the effects of the glycerol equilibration methods and embryo grades before freezing on the survival rate after thawing in mouse embryos. The results obtained from this study were as follows: 1. The number of embryos of grade I(Excellent), II(Good) and III(Fair) before freezing in this study was 97(27.4%), 160(45.2%) and 97(27.4%), respectively. 2. The average survival rate of frozen-thawed embryos in 3 and 5 steps glycerol equilibration was 66.7% and 64.1%, and the rate of transfererable embryos after culture was 68.9% and 69.0%, respectively. 3. Out of embryo grade I and II before freezing, the transferable rate after thawing was 75.2% and 48.1%, respectively, and grade I embryos before freezing was higher transferable rate than that of grade II.

• Journal of the Korean Geotechnical Society
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• v.19 no.5
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• pp.69-78
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• 2003

In order to investigate the dynamic behavior of weathered granite soils before and after freezing-thawing, cyclic triaxial tests were conducted for the specimens not only with the variation of silt contents within 20% but with plasticity index within 20%. As the results, the dynamic shear modulus of weathered granite soils decreased with increasing silt contents. However, the change in damping ratio was negligible. The influence of freezing-thawing on shear modulus and damping ratio was minimal for the granite soils with variation of silt contents. For the case of the weathered soils with variation of plasticity index, the shear modulus increased with plasticity index within 20%, while the modulus decreased remarkably after freezing-thawing.

• Proceedings of the Korea Concrete Institute Conference
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• 1997.10a
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• pp.293-298
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• 1997

Corrosion of steel reinforcing in concrete deteriorated by freezing/thawing and carbonation was characterized. Concrete specimens were prepared using various kinds of cements such as ordinary portland cement (type I), low heat portland cement (type IV, belite rich cement), sulphate resistance portland cement (type V), blast furnace slag portland cement and ternary blended cement. Of various cements, type V and type IV with lower $C_3A$ content revealed better steel corrosion resistance after freezing/thawing and carbonation. $C_3A$ content in cement might affect freezing/thawing resistance in sea water.

• Korean Journal of Animal Reproduction
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• v.18 no.1
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• pp.15-23
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• 1994

This study was carried out to investigate the effects of concentration, kinds of cryoprotectants, equilibration time, optimum thawing temperature on the survival rate of rapidly frozen porcine embryos. The porcine embryos following dehydration by cryoprotectants containing sucrose were directly plunged into liquid nitrogen and thawed in 30, 35 or 37$^{\circ}C$ water bath, Survival rate was defined as development rate on in vitro culture or FDA-test. The results are summarized as follows : 1. The high survival rate of porcine frozen embryos after rapidly thawed in freezing medium was attained 2.0M DMSO, 2.0M glycerol, 2.0M propanediol, 1.5M ethyleneglycol. 2. The high survival rate of porcine frozen embryos after rapidly thawed in freezing medium was obtained using single cryoprotectant(16.6~40.0%) than mixed cryoprotectants(12.5~33.3%). 3. The eqilibration time on the survival rate of rapidly thawed porcine frozen embryos was attained after short period of time(15.0~33.3%) in the freezing medium higher than long period of time(9.10~30.0%). 4. The thawing temperature on the survival rate of rapidly thawed porcine frozen embryos was attained at 3$0^{\circ}C$ of thawing temperature(33.3~40.6%) in the freezing medium higher than 25 or 37$^{\circ}C$ of thawing temperature.

• Geomechanics and Engineering
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• v.23 no.2
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• pp.179-187
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• 2020

Freezing-thawing cycles have significant effect on soils engineering behavior in frozen areas. This effect is more considerable in fine-graded than coarse-grained soils. The objective of this study is improving soil durability and strength in continues freezing-thawing cycles. For getting this purpose mixture of Polyvinyl Acetate (PVAc) and Ethylene Glycol Monobutyl Ether (EGBE) has been added to fine-grained soil and final prepared samples were tested at different freezing-thawing cycles. PVAc was mixed with 1%, 2% and 3% of soil weight. Half of PVAc weight was used as weight of EGBE. Freezing-Thawing cycles were exposed to samples and they were tested at different cycles. Results showed that adding mixture of PVAc+EGBE improved strength and durability of samples up to 10 freezing-thawing cycles. Unconfined compress strength tests were applied to samples and stress and strain of samples were tested on failure time. Behavior of samples was different at different percentages of mixture. Results showed that increasing amount of PVAc from 1% to 2% had more considerable effect on final stress than 2% to 3%. Using higher percentages of PVAc + EGBE mixture leaded to that samples carried more strain before collapsing. Another result gained from tests was that, freezing-thawing effect was more considerable after fourth cycles. It means differences between first and fourth cycles were more considerable than differences between fourth and tenth.

• Journal of the Korean Society of Food Science and Nutrition
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• v.19 no.2
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• pp.175-179
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• 1990

Investigations of skin stripping freezing and thawing conditions of common-European squid (Loigo vulgaris) were carried out. The most effective method of skin stripping was to immerse the sample at 5$0^{\circ}C$ for 10-15 minutes in fresh water or salt solution(5-10% w/v) Contact freezing and fresh water thawing method was found to be effective. The muscle structure of the sample thawed after contact freezing was almost the sample after contact freezing was almost the same as that of raw sample.

• Korean Journal of Veterinary Research
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• v.33 no.1
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• pp.171-178
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• 1993

The present study was carried out to investigate the developmental potency to blastocyst after freezing and thawing of nuclear transplanted 2-cell embryos. The nuclei from 2-, 4- and 8-cell mouse embryos were transferred into enucleated 2-cell embryos, and the reconstituted embryos were submitted to direct current(DC) pulse at output voltage of 2.0 kV/cm for $100{\mu}$ sec to induce cell fusion. The recovery rate and developmental potency to blastocyst after freezing and thawing of nuclear transplanted 2-cell embryos was investigated. 1. The recovery rate of nuclear transplanted 2-cell embryos in normal morphology after freezing and thawing was significantly higher in rapid freezing(DMSO 4.5M) than in slow cooling(p<0.01). 2. When the recovered embryos in normal morphology were cultured in vitro, there were no significant differences in the developmental potency to blastocyst between the freezing methods and the concentrations of cryoprotectant. In summary, these experiments have proved that rapid freezing method(DMSO 4.5M) is effective in nuclear transplanted 2-cell mouse embryos. If improved micromanipulation techniques and freezing are combined, nuclear transplantation technique will contribute to the improvement of productivity in livestock animals.