• Title/Summary/Keyword: test strip

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Development of a Test Strip Reader for a Lateral Flow Membrane-based Immunochromatographic Assay

  • Park, Je-Kyun;Kim, Suhyeon
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.9 no.2
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    • pp.127-131
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    • 2004
  • A low-cost, simple strip reader system using a linear movement mechanism of CD-ROM deck has been developed to characterize a lateral flow membrane-based immunochromatographic assay. The test strip reader was assembled by a CD-ROM deck and home-made optical head especially designed for immunoassays. The optical head for detecting reflected light from the test strip surface consists of green light-emitting diode, large area silicon photodiode, and anodized aluminum mounting block providing a slit structure for cutting light from the LED. The stepping motor of the deck was operated in the full step mode, whose distance of each reading point is about 0.15mm. The performance of the strip reader was tested by analysis of HBV(hepatitis B virus) antigen test kit. This strip reader can be useful for inexpensive, disposable, and membrane-based assays that provide visual evidence of the presence of an analyte in a liquid sample.

The correlation between dental caries experience and improved dental caries activity tests for the students of dental hygiene (치위생과 학생의 치아우식경험도와 개량형 우식활성검사와의 관련성)

  • Cho, Min-Jung;Kim, Jin-Hee;Kim, Eun-Mi;Lee, Hyang-Nim
    • Journal of Korean society of Dental Hygiene
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    • v.3 no.2
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    • pp.197-208
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    • 2003
  • This study was implemented for 84 students of dental hygiene to show the correlation between dental caries experience and improved caries activity test. Dental caries experience for the sample groups was examined and stimulative saliva secreted for 5 minutes was collected into the tube to check saliva secretion rate. Dentocult LB test was executed to observe Lactobacilli colonies after 96 hour cultivation of culture slides moistened with stimulative saliva. Dentocult SM test(screening strip, site strip) was done to measure SM colonies distribution after 48 hour cultivation of culture strips applied with collected saliva and dental plaque respectively, and salivary buffering capacity was checked by means of Dentobuff strip kit. Following conclusions are obtained after examining the relation between Dentocult LB, Dentocult SM, Dentobuff strip test results and DMFT index, salivary secretion rate. 1. Showed no significant difference between Dentocult LB test results and DMFT index, salivary secretion rate. 2. Showed no significant difference between Dentocult SM(screening strip) test results and DMFT index, salivary secretion rate. 3. Showed significant difference between Dentocult SM(site strip) test results and DMFT index(pE0.05), but showed no significant difference between Dentocult SM(site strip) test results and salivary secretion rate. 4. Showed no significant difference between Dentobuff strip test results and DMFT index, but showed a very wide difference between Dentobuff strip test results and salivary secretion rate(pE0.01).

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Development of Immunochromatography Strip-Test Using Nanocolloidal Gold-Antibody Probe for the Rapid Detection of Aflatoxin B1 in Grain and Feed Samples

  • Shim, Won-Bo;Yang, Zheng-You;Kim, Jung-Sook;Kim, Ji-Young;Kang, Sung-Jo;Woo, Gun-Jo;Chung, Young-Chul;Eremin, Sergei A.;Chung, Duck-Hwa
    • Journal of Microbiology and Biotechnology
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    • v.17 no.10
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    • pp.1629-1637
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    • 2007
  • An immunochromatography (ICG) strip test using a nanocolloidal gold-antibody probe was developed and optimized for the rapid detection of aflatoxin B1 (AFB1). A monoclonal antibody specific to AFB1 was produced from the cloned hybridoma cell (AF78), coupled with nanocolloidal gold, and distributed on the conjugate pad of the ICG strip test. The visual detection limit of the ICG strip test was 0.5 ng/ml, and this method showed a cross-reaction to aflatoxin B2, G1, and G2. In total, 172 grain and feed samples were collected and analyzed by both the ICG strip test and HPLC. The results of the ICG strip test showed a good agreement with those obtained by HPLC. These results indicated that the ICG strip test has a potential use as a rapid and cost-effective screening tool for the determination of AFB1 in real samples and could be applied to the preliminary screening of mycotoxin in food and agricultural products, generating results within 15 min without complicated steps.

The Test Strip Reflectometer Method as a Quick Test Procedure for Soil Nitrate Nitrogen (토양의 질산태 질소 간이검정)

  • Hong, Soon-Dal;Park, Hyo-Taek
    • Korean Journal of Soil Science and Fertilizer
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    • v.33 no.5
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    • pp.369-375
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    • 2000
  • To find a quick test procedure for soil nitrate concentration, two methods, i.e. "Nitracheck 404" reflectometer (Eijkelkamp, Netherlands) with nitrate test strip and a portable colorimeter "Hanna Ion Specific meter(USA) with a color development reagent, were tested for twenty soils with different nitrate contents ranged between from $10mg\;kg^{-1}$ to $340mg\;kg^{-1}$. The standard deviation, coefficient of variability, and recovery from these quick test procedures were compared with those measured by conventional Kjeldahl distillation method and nitrate ion electrode method. The nitrate concentration measured by portable colorimeter method was higher in soils with low concentration and lower in soils with high concentration than those measured by conventional methods. However, concentrations measured by test strip reflectometer method was showed the similar coefficient of variability and recovery as those by conventional methods for all soil samples. From the experimental results in this study, the test strip reflectometer method was thought to be recommendable one revealed the satisfied results for accuracy, quickness, and simplicity for field test of soil nitrate concentration.

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Development and validation of strip bending tester for measuring mechanical properties of freestanding thin films (자유지지 박막의 기계적 물성 측정을 위한 띠굽힘시험기의 개발 및 검증)

  • Park, Jung-Min;Kim, Jae-Hyun;Lee, Hak-Joo
    • Proceedings of the KSME Conference
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    • 2008.11a
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    • pp.49-55
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    • 2008
  • Strip bending test has been frequently utilized to measure the mechanical properties of freestanding thin films in substitute for the micro-tensile test. However, in spite of its simplicity and reliability, strip bending test has a few problems, for example, the measurement of strain and the calculation of stress at zero strain. In this study, these problems are precisely reviewed and proved. Upon this review, strip bending tester has been developed, which uses the confocal laser displacement meter to measure the deformed configuration of the specimen and the possibility and limitation of this testing system is carefully investigated including the estimation of uncertainty of the measurement of strain. Finally, to prevent errors and to improve the accuracy of this testing system, the shape of the specimen has been carefully studied and is proposed.

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DEVELOPMENT OF EVALUATION METHOD FOR FRICTIONAL CHARACTERISTICS OF ZINC COATE STEEL SHEET

  • Kim, Young-Suk-
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 1992.03a
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    • pp.71-82
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    • 1992
  • The frictional characteristics of Zn-Ni coated steel sheets were investigated by draw bead test and strip draw test. In strip draw test, the frictional characteristics were evaluated by the drawing force ratio (Tc/Ts) for half coating-stripped specimens. It is clarified that the drawing force ratio obtained by strip draw test is a convenient parameter compared to coefficient of friction obtained by draw bead test to evaluate the frictional characteristics of Zn-Ni coated steel sheets.

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Effect of Heat Treatment on the Tensile Deformation Behavior of Au-Sn Strip Manufactured by Strip Casting Process (박판 주조법으로 제조된 Au-Sn 스트립의 열처리에 따른 인장 변형 거동)

  • Lee, Kee-Ahn;Jin, Young-Min;NamKung, Jung;Kim, Mun-Chul
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2009.10a
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    • pp.464-466
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    • 2009
  • This study tried to examine the suitability of strip casting process such as PFC (Planar Flow Casting) method for soldering Au-Sn strip. The effect of heat treatment on the tensile behavior and mechanical properties of an Au-Sn strip was investigated through tensile test, micro hardness test, X-ray diffraction (XRD), SEM, and TEM observations. It was apparent that 20-mm width Au-Sn strip could be well produced by using planar flow casting process. Tensile results showed that tensile strength increased from 338.3MPa to 310MPa and plastic strain improved from 0% to 1.5% with heat treatment ($170^{\circ}C$/70 hrs.). The microstructure of Au-Sn strip mainly consisted of two phases; $Au_5Sn(\zeta)$ and AuSn($\sigma$). It was also found that inhomogeneous amorphous local structure continuously changed to the homogeneous two phases microstructure with heat treatment. The fractographical observation after tensile test indicated the cleavage fracture mode of as-casted Au-Sn strip. On the other hand, the heat treated Au-Sn strip showed that fracture propagated along interface between brittle AuSn and ductile $Au_5Sn$ phases. The deformation behavior of strip casted Au-Sn alloy with microstructural evolution and the improve method for ductility of this alloy was also suggested.

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Mechanical characterization of 100 nm-thick Au thin film using strip bending test (띠 굽힘 시험을 통한 100 nm 두께 금 박막의 기계적 특성 평가)

  • Kim, J.H.;Lee, H.J.;Han, S.W.;Baek, C.W.;Kim, J.M.;Kim, Y.K.
    • Proceedings of the KSME Conference
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    • 2004.04a
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    • pp.252-257
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    • 2004
  • Nanometer-sized structures are being applied to many devices including micro/nano electronics, optoelectronics, quantum devices, MEMS/NEMS, biosensors, etc. Especially, the thin film with submicron thickness is a basic structure for fabricating these devices, but its mechanical behaviors are not well understood. The mechanical properties of the thin film are different from those of the bulk structure and are difficult to measure because of its handling inconvenience. Several techniques have been applied to mechanical characterization of the thin film, such as nanoindentation test, micro/nano tensile test, strip bending test, etc. In this study, we focus on the strip bending test because of its high accuracy and moderate specimen preparation efforts, and measure Au thin film, which is a very popular material in micro/nano electronic devices. Au film is deposited on Si substrate by evaporation process, of which thickness is 100nm. Using the strip bending test, we obtain elastic modulus, yield and ultimate tensile strength, and residual stress of Au thin film.

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Determination of Aflatoxin B1 in Rice, Barley, and Feed by Non-instrumental Immunochromatographic Strip-test and High Sensitive ELISA

  • Shim, Won-Bo;Kim, Jung-Sook;Kim, Ji-Young;Choi, Jin-Gil;Je, Jung-Hyun;Kuzmina, Nina Sergeevna;Eremin, Sergei Alexandrovich;Chung, Duck-Hwa
    • Food Science and Biotechnology
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    • v.17 no.3
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    • pp.623-630
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    • 2008
  • A non-instrumental immunochromatographic (ICG) strip-test and direct competitive enzyme-linked immunosorbent assay (DC-ELISA) for aflatoxin B1 (AFB1) determination were developed and optimized. The detection limits of ICG strip-test and DC-ELISA were 0.5 and 0.004 ng/mL, respectively, and these methods possessed a cross-reaction to aflatoxins. The results of spiked samples by both methods were coincided with the amount spiked AFB1 and the comparative analyses of 172 real samples by 2 immunoassays and high performance liquid chromatography (HPLC) showed a good agreement. Especially, the ICG strip-test is easier to perform and quicker, but less sensitivity than DC-ELISA. Both methods could analyze a high sample throughput with short time, but the sample throughput of ICG strip-test was better. Therefore, the ICG strip-test can be used as a simple, easy, non-instrumental, and fast screening technique for AFB1 determination.

A Study on the Development of Microorganism Test Strips (대장균군 검사용 간이 시험지 개발)

  • 이인애;김재화;이희구;성창근;최인성;정태화
    • Biomedical Science Letters
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    • v.2 no.1
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    • pp.49-55
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    • 1996
  • The objective of this study was to develop a paper strip which could determine E. coli qualitatively and quantitatively in water, wastewater, drinks, or food. This paper strip method was a simple and rapid test method that determine E. coli by visual identification. In this study, nutrient culture media were formulated and characterized for optimum conditions. Paper strips were then prepared by impregnating into the media and dried at $60^\circ$C. The test procedure is quite simple to use. The paper strip was dipped into a sample, and excess sample was removed. The strip was then incubated at $37^\circ$C for 16 to 20 hours and the number of colonies on the strip was counted. The color of the colony spots produced by microorganisms varied depending on the media formulation. Violet-red spots were produced by E. coli. The test method was simple, rapid and no special laboratory equipment was necessary for visual identification. Therefore, this test method is applicable to on-site tests such as field tests or home tests. The paper strip method was compared with the standard agar plate method and Japanese commercial product. The method of the economical preparation of test strips was studied for production on industrial scale.

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