• 한국콘텐츠학회논문지
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• 제20권4호
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• pp.454-463
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• 2020

본 연구는 최근 인간의 건강을 위협하는 다양한 원인 중에서 우리 주변에서 흔히 접할 수 있는 대상을 통해 그 원인의 해결을 제안하고자 시도 되었다. 천연물 유래의 테르펜의 경우 기존의 피톤치드와 같은 역할을 하고 있으며 다양한 생리활성 기능을 수행하고 있다. 본 연구에서는 우리주변에서 용이하게 구할 수 있으며 적용이 가능한 생물소재를 통해 오염원을 제거할 수 있는 생리활성을 검증하였다. 본 연구에서는 계피오일과 테르펜을 이용하여 다양한 질병과 오염원이 되고 있는 진드기와 미생물에 대한 효능을 검증하였다. 본 실험에서는 우리가 흔히 사용하는 베개를 이용하여 베게 등과 같은 침구류에서 발생할 수 있는 다양한 미생물과 진드기의 발생에 대한 작용에 대해 그 효능을 검증하였다. 그 결과로 테르펜의 항 미생물 활성은 Pour plate 방법을 사용하여 관찰하였다. 항진드기 효과는 테르펜을 사용하지 않고 첫 번째 테스트에서 베개 4개 중 2개에서 약한 양성(100 미만)으로 감지되었다. 테르펜을 사용한 두 번째 실험에서, 모든 시료들이 20 미만으로 음으로 감소되는 것으로 확인되었으며, 테르펜의 최상의 항진드기 효능은 20%인 것으로 밝혀졌다. 이 연구의 목적은 천연물 유래의 테르펜을 이용한 침구용 항균 및 진드기 방지 제품의 개발 가능성을 제안하는 것이다.

• 한국식품영양과학회지
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• 제31권1호
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• pp.26-31
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• 2002

Pinus densiflora(2엽송) 1년생을 채취하여 가지, 잎, 새순 부분으로 구분하고, 시료병에 넣어 밀봉한 뒤, solide phase microextraction (SPME)과 dynamic headspace analysis(DHA)를 이용하여 휘발성 성분을 분리, 동정하였다. SPME와 DHA의 결과를 terpenes의 구성 성분에 따라 monoterpenes과 sesquiterpenes, oxygenated terpenes으로 나누어 조성비를 살펴보면, monoterpenes의 조성은 잎의 경우 각각 66.7%와 14.3%, 새순은 90.6%와 0.7%, 가지에서는 90.6%와 1.2%로 나타났다. Sesquiterpenes의 함량은 SPMe의 결과에서만, 잎이 25.8%, 가지 4.4%, 새순 1.5%로 나타났다. 산소를 함유하고 있는 terpenes의 함량은 새순 부위가 4.7%, 79.0%로 나타났으며, 가지 부위는 3.7%와 70.4%, 잎에서 1.0%와 50.7%를 각각 차지하였다. SPME의 결과는 이전의 용매추출을 통한 결과와 다소 비슷한 양상을 보였으며, DHA의 결과는 10$0^{\circ}C$이상의 고비점 화합물들이 나타나지 않은 반면, 5$0^{\circ}C$이하의 저비점 화합물이 15개 분리되었다.

• Journal of Microbiology and Biotechnology
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• 제12권4호
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• pp.610-616
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• 2002

The growth-inhibiting effects of Pinus densiflpora leaf-derived materials on nine human intestinal bacteria were investigated using the impregnated paper disk method, and their activities were compared with those of 13 commercially available terpenes. The biologically active constituent of the extract of P densiflora leaf was characterized as the monoterpene (1R)-(+)-$\alpha$-pinene by various spectroscopic analyses. Responses varied according to bacterial strain, chemicals, and dose. At 10 mg/disk, limonene and (1R)-(+)-$\alpha$-pinene strongly inhibited the growth of Clostridium perfringens, Staphylococcus aureus, and Escherichia coli, without adverse effects on the growth of five lactic acid-bacteria (Bifidobacterium adolescentis, B. bifidum, B. longum, Lactobacillus acidophilus, and L. casei). Little or no inhibition against seven bacteria was observed with anethole, borneol, camphor, caryophyllene, 1,8-cineole, estragole, linalool, and $\alpha$-terpineol. Structure-activity relationship revealed that (1R)-(+)-$\alpha$-pinene had more growth-inhibiting activity against C. perfringens than (1R)-(+)-$\beta$-, (1S-(-)-$\alpha$-, and (1S-(-)-$\beta$-pinenes. Furthermore, the growth-inhibition against L. casei was much more pronounced in (1R)-(+)-$\beta$- and (In-(-)-$\beta$-pinenes than (1R)-(+)-$\alpha$- and (1S)-(-)-$\alpha$-pinenes. These results indicate that the (+)-$\alpha$ form seems to be required against C. perfringens and $\beta$ form against L. casei for growth-inhibiting activity. Morphologically, most strains of C. perfringens were damaged and disappeared at 5 and 2 mg/disk of (1R)-(+)-$\alpha$-pinene. Morphological study revealed that (1R)-(+)-$\alpha$-pinene had more growth-inhibiting activity against C. perfringens than (1R)-(+)-$\beta$-, (1S)-(-)-$\alpha$-, and (1S)-(-)-$\beta$-pinenes. As naturally occurring growth-inhibiting agents, the Pinus leaf-derived materials described above could be useful preventive agents against diseases caused by harmful intestinal bacteria such as clostridia.

• 한국식품영양과학회지
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• 제25권4호
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• pp.654-658
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• 1996

제호탕의 유기산 함량은 formic acid 739.Snm(0.07%), lactic acid 2162.1ppm(0.22%), acetic acid 3204ppm(0.32%), citric acid 31763.7ppm(3.2%)이었으며 가장 특이한 것은 citric acid가 3.2%를 함유하는 것으로 나타나 제호탕의 신맛에 근원인 것으로 생각되었다. GC/MSD사용하여 확인된 제호탕의 향기성 분은 39종으로 이들의 구성 성분을 분류하면 hydrocarbon류(30.81%) 17종, adehyde류(7.18 %) 1종, ketone류(4.79%) 2종, terpene류(25.96%) 8종, acid류(16%) 4종, alcohol류(5.42%) 2종, phenol류(2.76%) 2종, 그리고 기타 향기(7.68%) 3종이었다. 이 결과로 볼 때 hydrocarbon류, terpene류, acid류가 전체 향기성분의 70%이상을 차지하여 이들이 제호탕의 특유한 향기성분이었다. 제호탕의 색소 추출액은 200~400nm에서 최대 홉수파장을 나타내어 황색~적색 계통의 여러가지 색소가 혼합되어 있음을 알 수 있었다.

• 한국식품영양학회지
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• 제16권4호
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• pp.372-378
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• 2003

SDE 및 SPME를 이용하여 계피로부터 추출한 휘발성 향기성분을 GC/MSD 분석한 결과 terpenes 20종, alcohol 3종, carbonyl 5종, esters 2종으로 총 30개의 휘발성 성분을 확인하였다. 이들 중 trans-cinnamaldehyde (86.4%), cis-cinnamaldehyde(0.9％), hydrocinnamaldehyde (0.1%) 등의 carbonyl 화합물이 peak area% 기준으로 약 88.2%로 계피 중 대부분을 차지하는 것으로 나타났다. 또한 식물체의 향기성분 분석에 많이 이용되는 SPME fiber 4종류를 비교 분석한 결과 terpen류 화합물들은 PDMS fiber에서 추출효율이 가장 좋았으며, cinnamyl alcohol과 같은 다소 극성인 화합물의 경우 DVB/CAR/PDMS fiber와 PA fiber에서 추출 효율이 가장 좋은 것으로 나타났다. SPME법에서 최적 조건을 설정하기 위해 추출온도와 추출시간을 달리하면서 비교 분석한 결과 copaene, murolene, cadinene, cis-cinnamaldehyde 등의 area% 값은 온도와 시간이 커질수록 증가한 반면에 trans-cinnamaldehyde의 area% 값은 감소하였다.

• Applied Biological Chemistry
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• 제41권1호
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• pp.84-88
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• 1998

밤꿀과 밤꽃 향기성분의 연관성을 조사코자 밤꿀은 용매추출과 수증기 증류법을 병행하여, 그리고 밤꽃은 수증기 증류법에 의해 향기성분을 분리한 다음 GC, GC-MS에 의해 확인 비교하였다. 밤꽃으로 부터 14종의 방향족 화합물, 13종의 탄화수소류, 7 종의 지방산류, 4종의 테르펜류, 12종의 함산소 탄화수소류, 7종의 기타 성분 등 64종의 성분이 확인되었고, 밤꿀로 부터는 7종의 방향족화합물, 16종의 탄화수소류, 12종의 지방산류, 1종의 테르펜류, 2종의 함산소 탄화수소류, 3종의 기타 성분 등 41종이 확인되었다. 밤꽃 향기의 주요성분으로는 전체 향기의 49.02%를 차지하고 있는 2-phenyl ethyl alcohol, 1-phenyl ethyl alcohol, benzyl alcohol 등이 주를 이루었고 밤꿀 향기의 특징적인 성분으로는 2-phenyl ethyl alcohol, benzyl alcohol, 1-phenyl ethyl alcohol, 1-(2-aminophenyl) ethanone 등이었다.

• 한국수산과학회지
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• 제53권3호
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• pp.259-272
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• 2020

Nowadays, two types, Yumhae and Sikhae methods, remained as traditional seafood fermentation methods in Korea. In this study, flavor compounds in two types of salt-fermented fishes made by Yumhae method such as anchovy Engraulidae sp., shrimp Caridea sp., squid Decapodiformes sp., big eyed herring Clupea sp., gizzard shad Dorosoma sp. and hairtail Trichiurus sp., and made by Sikhae method such as Alaska pollack Gadus Chalcogrammus and squid. Volatile compounds detected in all salt-fermented fishes were composed mainly of aldehydes (45), ketones (39), alcohols (45), acids (12), esters (47), N-containing compounds (43), aromatic hydrocarbons (37), S-containing compounds (26), furans (10), and miscellaneous compounds (40) in salt-fermented fishes made by Yumhae method. Meanwhile, alcohols (47), terpenes (38), S-containing compounds (22), carbonyl compounds (19 aldehydes, 18 ketones), esters (13), and acids (14). Aroma-active compounds were identified by Gas chromatography/mass spectrometry/olfactometry and aroma extract dilution analysis in salt-fermented anchovy, shrimp and tuna (Thunnini sp.) sauce. Ethyl 2-methylbutanoate (candy/sweet) and 2-ethyl-3,5-dimethylpyrazine (nutty/baked potato-like) were predominant odorants in salt-fermented anchovy, whereas dimethyl trisulfide (cooked cabbage/soy sauce-like), 2-ethyl-3,5-dimethylpyrazine, and (E,E)-2,4-heptadienal (fatty/grainy) in salt-fermented shrimp, and dimethyl trisulfide, 3-methylbutanal (dark chocolate-like), and 3-methylthiopropanal (baked potato-like) in tuna sauce.

• Mycobiology
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• 제44권3호
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• pp.162-170
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• 2016

In this paper, we demonstrate the ability of Arabidopsis thaliana to detect different mixtures of volatile organic compounds (VOCs) emitted by the common indoor fungus, Aspergillus versicolor, and demonstrate the potential usage of the plant as a bioindicator to monitor fungal VOCs in indoor air. We evaluated the volatile production of Aspergillus versicolor strains SRRC 108 (NRRL 3449) and SRRC 2559 (ATCC 32662) grown on nutrient rich fungal medium, and grown under conditions to mimic the substrate encountered in the built environment where fungi would typically grow indoors (moist wallboard and ceiling tiles). Using headspace solid phase microextraction/gas chromatography-mass spectrometry, we analyzed VOC profiles of the two strains. The most abundant compound produced by both strains on all three media was 1-octen-3-ol. Strain SRRC 2559 made several terpenes not detected from strain SRRC 108. Using a split-plate bioassay, we grew Arabidopsis thaliana in a shared atmosphere with VOCs from the two strains of Aspergillus versicolor grown on yeast extract sucrose medium. The VOCs emitted by SRRC 2559 had an adverse impact on seed germination and plant growth. Chemical standards of individual VOCs from the Aspergillus versicolor mixture (2-methyl-1-butanol, 3-methyl-1-butanol, 1-octen-3-ol, limonene, and ${\beta}-farnesene$), and ${\beta}-caryophyllene$ were tested one by one in seed germination and vegetative plant growth assays. The most inhibitory compound to both seed germination and plant growth was 1-octen-3-ol. Our data suggest that Arabidopsis is a useful model for monitoring indoor air quality as it is sensitive to naturally emitted fungal volatile mixtures as well as to chemical standards of individual compounds, and it exhibits relatively quick concentration- and duration-dependent responses.

• 한국식품영양학회지
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• 제31권5호
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• pp.703-711
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• 2018

This study was carried out to investigate quality characteristics of Korean domestic commercial meoru wines, 8 kinds of wines were purchased from the Korea Wine Festival in 2016 and we compared the characteristics and physiological activity of 8 domestic wine. The results of this study have shown that the alcohol contents of wines ranged from 9.8 to 14.3%, pH of wines ranged from 3.86 to 4.22 and the total acidity of wines ranged from 0.56~0.75%. The hue value of meoru wines ranged from 0.81 to 1.02, The brightness of meoru wines ranged from 0.77 to 5.55, the redness from 3.97 to 31.16, and the yellowness from 0.99 to 5.63. The organic acid analysis of wine revealed lactic acid content at 4.281~9.606 mg/mL, followed by malic acid, tartaric acid, and acetic acid. The concentrations of total polyphenol and anthocyanin contents in the samples were investigated by spectrophotometric methods. Total polyphenol contents of the M8 (172.24 mg%) wine was higher than those of the other wines and total anthocyanin contents represented from 356.69 to 601.33 mg/mL. The DPPH radical scavenging activity of meoru wines was between 57.23 to 73.98%. Volatile flavor component analysis of meoru wines identified 7 alcohols, 16 esters, 5 acids, 3 terpenes and 4 other compounds.

• 한국응용과학기술학회지
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• 제31권2호
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• pp.285-295
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• 2014

Citrus essential oil were extracted from citron peel of cultivated in Gohong (CCP) using environmentally friendly supercritical carbon dioxide method. Antioxidant activity and aroma composition of the essential oils extracted by the SC-$CO_2$ method were evaluated by comparing with those extracted by organic solvent method. Fatty acid composition, DPPH scavenging, and antioxidant activity of the oils obtained by different extraction condition of SC-$CO_2$ method were investigated and their antioxidant activities were compared with commercially available lavender, eucalyptus and tea tree oils. As the results, linoleic acid was most abundantly found from CCP oil extracted by the SC-$CO_2$ method and cis-11,144-eicosadienoic acids was second abundantly found. Radical scavenging ability of DPPH was 98% when the concentration of CCP oil was 50 mg/mL. This scavenging ability increased with the increase of oil concentration. On the other hand, flavonoids content (84 mg/100g) of the CCP oil extracted by the SC-$CO_2$ method was slightly higher than that (75 mg/100g) by hexane extraction. The results, obtained from CCP oil by GC-MS, indicated that, among 66 components, the content of terpenes was 55.8% and limonene was 27.3%.