• 제목/요약/키워드: ternary complex

검색결과 84건 처리시간 0.024초

Interaction Between Acid-Labile Subunit and Insulin-like Growth Factor Binding Protein 3 Expressed in Xenopus Oocytes

  • Choi, Kyung-Yi;Lee, Dong-Hee
    • BMB Reports
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    • 제35권2호
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    • pp.186-193
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    • 2002
  • The acid-bible subunit (ALS) associates with the insulinlike growth factor (IGF)-I or II, and the IGF binding protein-3 (IGFBP-3) in order to form a 150-kD complex in the circulation. This complex may regulate the serum IGFs by restricting them in the vascular system and promoting their endocrine actions. Little is known about how ALS binds to IGFBP3, which connects the IGFs to ALS. Xenopus oocyte was utilized to study the function of ALS in assembling IGFs into the ternary complexes. Xenopus oocyte was shown to correctly translate in vitro transcribed mRNAs of ALS and IGFBP3. IGFBP3 and ALS mRNAs were injected in a mixture, and their products were immunoprecipitated by antisera against ALS and IGFBP3. Contrary to traditional reports that ALS interacts only with IGF-bound IGFBP3, this study shows that ALS is capable of forming a binary complex with IGFBP3 in the absence of IGF When cross-linked by disuccinimidyl suberate, the band that represents the ALS-IGFBP3 complex was evident on the PAGE. IGFBP3 movement was monitored according to the distribution between the hemispheres. Following a localized translation in the vegetal hemisphere, IGFBP3 remained in the vegetal half in the presence of ALS. However, the mutant IGFBP3 freely diffused into the animal half, despite the presence of ALS, which is different from the wild type IGFBP3. This study, therefore, suggests that ALS may play an important role in sequestering IGFBP3 polypeptides via the intermolecular aggregation. Studies using this heterologous model will lead to a better understanding of the IGFBP3 and ALS that assemble into the ternary structure and circulate the IGF system.

GaAs에서의 Si의 확산기구와 그에 관련된 격자 결함 화학 (Diffusion Kinetics of Si in GaAs and Related Defect Chemistry)

  • 이경호
    • ETRI Journal
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    • 제11권4호
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    • pp.75-83
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    • 1989
  • The diffusion mechanism of Si in GaAs was investigated using different diffusion sources based on the Si-Ga-As ternary phase equilibria. The Si profiles are measured with secondary ion mass spectrometry and differ significantly for sources taken from the different phase fields in the ternary phase diagram. Neutral As vacancy diffusion is proposed for acceptor Si diffusion anneals using a Ga - Si - GaAs source. Donor Si diffusion using As - rich sources and a Si -GaAs tie line source shows concentration dependent diffusion behavior. Concentration dependent diffusion coefficients of donor Si for As - rich source diffusion were found to be related to net ionized donor concentration and showed three regimes of different behavior: saturation regime, intermediate regime,and intrinsic regime. Ga vacancies are proposed to be responsible for donor Si diffusionin GaAs: $Si_Ga^+V_Ga^-$ (donor Si -acceptor Gavacancy) complex for the extrinsic regime and neutral $V_G$a, for the intrinsic regime.The Si - GaAs tie line source resulted in two branch profiles, intermediate between the As - rich and the Ga - rich source diffusion cases.

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Studies on the Ternary Blends of Liquid Crystalline Polymer and Polyesters

  • Kim, Seong-Hun;Kang, Seong-Wook
    • Fibers and Polymers
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    • 제1권2호
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    • pp.83-91
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    • 2000
  • Thermotropic liquid crystalline polymer made up of poly(p-hydroxybenzoate) (PHB)-poly(ethylene terephthalate)(PET) 8/2 copolyester, poly(ethylene 2,6-naphthalate) (PEN) and PET were mechanically blended to pursue the liquid crystalline phase of ternary blends. Complex viscosities of blends decreased with increasing temperature and PHB content. DSC thermal analysis indicated that glass transition temperature (Tg) and melting temperature (Tm) of blends increased with increasing PHB content. Both tensile strength and initial modulus increased with raising PHB content and take-up speed of monofilaments. In the WAXS diagram, only PEN crystal reflection at 2Θ=$15.5^{\circ}C$ appeared but PET crystal reflection was not shown in all compositions. The degree of transesterification and randomness of blends increased with blending time but sequential length of both PEN and PET segment decreased.

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뇌조직으로부터 정제한 Succinic semialdehyde reductase의 정제 및 활성기작 연구

  • 최수영;송민선;최의열;조성우
    • 한국응용약물학회:학술대회논문집
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    • 한국응용약물학회 1994년도 춘계학술대회 and 제3회 신약개발 연구발표회
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    • pp.271-271
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    • 1994
  • NADPH-dependent succinic semialdehyde reductase를 소의 뇌조직으로부터 여러가지 크로마토그래픽 방법을 이용하여 순수 분리 정제하였다. 효소는 분자량 34kDa을 가진 monomeric 단백질이며 substrate specificity. 분자량, 최적 pH, 아미노산 조성 등을 다른 sources의 효소들과 비교하였다. 이 비교 결과들로부터 본 연구에서 정제한 효소는 다른 sources와 다른 효소로 밝혀졌다. 반응의 산물이나 유사 기질 등을 저해제로 사용하였을때의 반응기작은 intermediate ternary complex를 형성하고 NADPH가 먼저 효소에 binding하는 ordered Sequencial mechanism을 보인다는 사실을 관찰할 수 있었다.

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NUMBER SYSTEMS PERTAINING TO EUCLIDEAN RINGS OF IMAGINARY QUADRATIC INTEGERS

  • Sim, Hyo-Seob;Song, Hyun-Jong
    • East Asian mathematical journal
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    • 제31권3호
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    • pp.357-362
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    • 2015
  • For a ring R of imaginary quadratic integers, using a concept of a unitary number system in place of the Motzkin's universal side divisor, we show that the following statements are equivalent: (1) R is Euclidean. (2) R has a unitary number system. (3) R is norm-Euclidean. Through an application of the above theorem we see that R admits binary or ternary number systems if and only if R is Euclidean.

Hydroperoxide 의존성 반응에서의 Cytochrome P-450의 산화활성종 형성양식 (Mechanism of Peroxide-supported Hydroxylation by Cytochrome P-450 : Its Formation Pattern of the Active Intermediate)

  • 문전옥;김기헌
    • 약학회지
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    • 제37권1호
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    • pp.95-99
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    • 1993
  • Peroxidase activity of cytochrome P-450 was examined using N, N-dimethylaniline (NDA) as a substrate and cumene hydroperoxide (CHP) as an oxidant. The initial rates of the N-demethylation for varied concentrations of NDA (0.05-0.5 mM) by P-450 at different fixed concentrations of CHP (0.02-0.2 mM) were determined. The results suggest that P-450 proceeds its peroxidative reaction by the rapid equilibrium random bi bi mechanism to form a ternary complex with substrate and oxidant as an active intermediate.

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The Oxidation of Hydrazobenzene by Oxygen Catalysed by Co (3MeOsalen) in Methanol

  • Homer Roger B.;Cannon Roderick D.;Kim Stephen S.B.
    • Bulletin of the Korean Chemical Society
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    • 제6권2호
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    • pp.115-118
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    • 1985
  • The oxidation of hydrazobenzene by oxygen in methanol solution is catalysed by Co(3MeOsalen) which is a synthetic oxygen carrier. The products are trans-azobenzene and water. The rate of the reaction has been studied spectrophotometrically and the rate law established. A mechanism involving a ternary complex of catalyst, hydrazobenzene and oxygen has been proposed.

고기능성 세라믹 주형 및 중자 제작을 위한 3원계 무기 바인더 시스템 개발 (Development of Ternary Inorganic Binder System for Manufacturing High-Functional Ceramic Molds and Core)

  • 박혜영;조근호;최현희;김봉구;김은희;양승철;정연길
    • 한국재료학회지
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    • 제32권12호
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    • pp.538-544
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    • 2022
  • In existing ceramic mold manufacturing processes, inorganic binder systems (Si-Na, two-component system) are applied to ensure the effective firing strength of the ceramic mold and core. These inorganic binder systems makes it possible to manufacture a ceramic mold and core with high dimensional stability and effective strength. However, as in general sand casting processes, when molten metal is injected at room temperature, there is a limit to the production of thin or complex castings due to reduced fluidity caused by the rapid cooling of the molten metal. In addition, because sodium silicate generated through the vitrification reaction of the inorganic binder is converted into a liquid phase at a temperature of 1,000 ℃. or higher, it is somewhat difficult to manufacture parts through high-temperature casting. Therefore, in this study, a high-strength ceramic mold and core test piece with effective strength at high temperature was produced by applying a Si-Na-Ti three-component inorganic binder. The starting particles were coated with binary and ternary inorganic binders and mixed with an organic binder to prepare a molded body, and then heat-treated at 1,000/1,350/1,500 ℃ to prepare a fired body. In the sample where the two-component inorganic binder was applied, the glass was liquefied at a temperature of 1,000 ℃ or higher, and the strength decreased. However, the firing strength of the ceramic mold sample containing the three-component inorganic binder was improved, and it was confirmed that it was possible to manufacture a ceramic mold and core via high temperature casting.

효율적인 IP 주소 검색을 위한 개선된 LC-trie (Improved LC-trie for Efficient IP Address Lookup)

  • 김진수;김정환
    • 한국콘텐츠학회논문지
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    • 제7권3호
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    • pp.50-59
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    • 2007
  • IP 주소 검색은 라우터에서 가장 중요하고 복잡한 기능중 하나이다. 본 논문에서는 고성능 라우터에서 IP 주소 검색의 성능을 향상시키기 위해 LC-trie를 개선하는 기법을 제안한다. TCAM(Ternary Content Addressable Memory)에서 테이블 압축을 위해 이용한 프리픽스 pruning 방법을 LC-trie에 효과적으로 적용한다. 이러한 기법은 메모리 참조 횟수를 감소시키고 검색의 속도를 높일 수 있다. 또한, 실제 사용되는 포워딩 테이블과 트래픽 분포를 사용하여 검색 시간과 메모리 참조 횟수 둥의 관점에서 개선된 기법의 성능을 기존의 LC-trie 기법과 비교 평가한다.

합성 Goethite에 의한 인산이온, 황산이온 및 구리이온의 혼합용액에서의 흡착특성 (Adsorption Characteristics by Synthesized Goethite in the Mixed Solution Systems of Phosphate, Sulfate, and Copper Ions)

  • 감상규;이동환;이민규
    • 한국환경과학회지
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    • 제12권10호
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    • pp.1055-1060
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    • 2003
  • Adsorption on goethite of individual component from a solution containing phosphate, sulfate, or copper ion was investigated. Competitive adsorption in the binary and ternary solution systems composed of phosphate, sulfate, and copper ions was also investigated. In competitive adsorption systems with phosphate and sulfate ions, the presence of phosphate ion reduced the adsorption of sulfate ion largely. On the other hand, the presence of sulfate ion caused only a small decrease in phosphate adsorption. This result suggests that phosphate ion is a stronger competitor for adsorption on goethite than sulfate ion, which is consistent with the higher affinity of phosphate for the surface compared to sulfate ion. Compared to the results from single-sorbate systems, adsorption of copper ion in the binary system of sulfate ion and copper ion was found to be enhanced in the presence of sulfate ion. Addition of sulfate ion to the binary system of copper ion and phosphate ion resulted in a small enhancement in copper sorption. This result implies that the presence of sulfate ion promotes adsorption of the ternary complex FeOHCuSO$_4$. The adsorption isotherms could be well described by the Langmuir adsorption equation.