• Title/Summary/Keyword: temperature-sensitive

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Study on the structure of cAMP receptor protein(CRP) by temperature change (온도변화에 의한 cAMP 수용성 단백질(CRP)의 구조)

  • 주종호;구미자;강종백
    • Journal of Life Science
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    • v.10 no.3
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    • pp.279-285
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    • 2000
  • CRP (cyclic AMP receptor protein) regulate transcription of catabolite-sensitive genes in Escherichia coli. Wild-type and mutant CRP (S83G and S128A) proteins were used to measure the thermal stability and the temperature-dependent structural change by proteolytic digestion, UV spectrophotometer and CD spectrapolarimeter. The result indicated that wild-type CRP was more thermally stable than the mutant CRPs in the presence of cAMP. At a low temperature, wild-type CRP with cAMP was more sensitive to subtilisin than the mutant CRPs. At a high temperature, there was no difference of sensitivity to subtilisin among wild-type, S83G and S128A CRPs. CD spectra suggested that the secondary structure of CRP was destroyed partially at a high temperature.

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Thermocontrol of Solute Permeation across Polymer Memberane Composed of Poly(N, N-dimethylaminoethyl methacrylate) and Its Copolymers

  • Yuk, Soon-Hon;Cho, Sun-Hang
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.6 no.4
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    • pp.274-278
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    • 2001
  • Polymer membranes composed of N, N-dimethylaminoethyl methaccrylate(DMAEMA) and acrylamide(AAm)(or ethyl acrylamide(EAAm) were prepared to demonstrated the thermo-control of solute permeation. Poly DMEMA has a lower critical solution temperature(LCST) at around 50$\^{C}$ in water, With the copolymerization of DMAEMA with AAm (or EAAm) a shift in the LCST to a lowere temperature was observed, probably due to the formation of hydrogen bonds between the amide and N-N-dimethylamino groups. However, the temperature-induced phase transition of poly(DMAEMA-co-EAAm) did not show a similar trend to that of poly(DMAEMA- co-AAm) in the gel state. The hydrogen bonds in poly(DMAEMA-co-EAAm) were significantly disrupted with the formation a gel network, which led to a difference in the swilling behavior of polymer gels in response to temperature. To apply these polymers to temperature-sensitive sol-ute permeation, polymer membranes were prepared. The permeation pattern of hydrocortisone, used as the model solute, was explained based on the temperature-sensitive swelling behavior of the polymer membranes.

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Observations of Coastal Upwelling at Ulsan in summer 1997

  • Lee, Jae-Chul;Kim, Dae-Hyun;Kim, Jeong-Chang
    • Journal of the korean society of oceanography
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    • v.38 no.3
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    • pp.122-134
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    • 2003
  • Low-pass filtered time series of wind, coastal temperature, sea level and current were analyzed to understand the coastal upwelling processes in the southeast coast of Korea. Southerly winds favorable for coastal upwelling were dominant in summer of 1997. Total period of four major wind events amounts to 58 days during one hundred days from June to early September. Coastal temperature is most sensitive to variations of wind. The time lag between the onset of southerly (northerly) winds and decrease (increase) of temperature is 3-18 hours. In the frequency domain the coherent bands have periods of 2.4 and 4.0-5.4 days with respective phase lags of 17 and 27-37 hours. Despite the sensitive response, the magnitude of temperature change is not quantitatively proportional to the intensity or duration of the wind, because it depends on the degree of baroclinic tilting of isotherms built dynamically by the strong Tsushima Warm Current (TWC). Current is particularly strong near the coast and has a large vertical shear during the upwelling periods, which is associated with the baroclinic tilting. Both of current and sea level are poorly coherent with wind or temperature except for the period of 4 days.

Climate Change Impacts on Optimum Ripening Periods of Rice Plant and Its Countermeasure in Rice Cultivation (기후변화에 따른 벼 적정 등숙기간의 변동과 대책)

  • 윤성호;이정택
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.3 no.1
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    • pp.55-70
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    • 2001
  • It was unusual crop weather for 1998 and 1999 compared with normal in Korea. The consecutive days of the optimum ripening period for rice plant that had daily mean temperature 21~23$^{\circ}C$ for 40 days after flowering, increased with long anomalies in 1998~99. The air temperature during ripening period was much higher than the optimum temperature and lower sunshine hour than norm in the local adaptability tests of newly developed rice lines during those years. In response of rice cultivation to warming and cloudy weather during crop season, the yield shall be decreased. Most scientists agree that the rate of heating is accelerating and temperature change could become increasingly disruptive. Weather patterns should also become more erratic. Agrometeorologists could be analyzed yearly variations of temperature, sunshine hour and rainfall pattern focused on transient agroclimate change for last a decade. Rice agronomists could be established taking advantage of real time agricultural meteorology information system for fertilization, irrigation, pest control and harvest. Also they could be analyzed the characteristics of flowering response of the recommended and newly bred rice cultivars for suitable cropping plan such as cultural patterns and sowing or transplanting date. Rice breeders should be deeply considered introducing the characteristics of basic vegetative type of flowering response like Togil rices as prospective rice cultivars corresponding to global warming because of the rices needed higher temperature at ripening stage than japonica rices, photoperiod-sensitive and thermo-sensitive ecotypes.

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Synthesis and Characterization of Temperature and pH Sensitive Graft Copolymers Based on Pluronic (Pluronic을 기초로 한 온도와 pH에 민감한 그래프트 공중합체의 합성과 특성)

  • Oh, Yeon-Jeong;Lee, Gi-Baek;Park, Sung-Young
    • Polymer(Korea)
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    • v.36 no.2
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    • pp.223-228
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    • 2012
  • Temperature and pH sensitive graft copolymers [Pluronic-$g$-poly(NIPAAm-$co$-MMA), Polymer A] and [Pluronic-$g$-poly( NIPAAm-$co$-MAA), Polymer C] were synthesized by macro radical graft polymerization with $N$-isopropylacrylamide (NIPAAM)/$N,N$-diethylaminoethylmethacrylate (DEAEMA) and $N$-isopropylacrylamide (NIPAAm)/methacrylic acid (MAA) based on Pluronic, respectively. The chemical structure and molecular weight of the graft copolymers was characterized by $^1H$ NMR and gel permeation chromatography. The aqueous solution properties of graft copolymers were measured using a UV-visible spectrophotometer, contact angle and dynamic light scattering equipment with different temperature and pH conditions. The obtained graft copolymers showed a very sensitive phase transition in response to temperature and pH in aqueous media which suggested that the amine group of DEAEMA segment and carboxylic group of MAA had a great influence on the lower critical solution temperatures (LCST) in Polymer A and C, respectively. The graft copolymers can be utilized for drug delivery system and molecular switching applications where responses to temperature and pH changes are relevant.

A Study on the Flow Behavior of Magnetic Fluids in a half Circular Pipe (반원관내 자성유체의 자연대류에 관한 연구)

  • Hwang, Sung-Wook;Park, Joung-Woo;Seo, Lee-Soo
    • Proceedings of the KSME Conference
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    • 2007.05b
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    • pp.3098-3103
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    • 2007
  • In this paper, natural convection of a magnetic fluids(W-40) in a half circular pipe enclosure are investigated by numerical and experimental method. One side wall is kept at a constant temperature(25$^{\circ}C$), and the opposite side wall is also kept at a constant temperature(20$^{\circ}C$). Under above conditions, various magnitudes of the magnetic fields were applied up. Theoretical study through the governing equation derived by Siliomis is carried out with numerical analysis by the GSMAC Method. And the thermo-sensitive liquid crystal film(R20C5A) is utilized in order to visualize wall-temperature distributions as an experimental method. This study has resulted in the following fact that the natural convection of a magnetic fluids are controlled by the direction and intensity of the magnetic fields.

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Temperature-dependent studies on catalytic hydrosilation of polyalkylsiloxane using NMR

  • Sul, Hyewon;Lee, Tae Hee;Lim, Eunsoo;Rho, Yecheol;Kim, Chong-Hyeak;Kim, Jeongkwon
    • Analytical Science and Technology
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    • v.30 no.4
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    • pp.213-219
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    • 2017
  • Polyalkylsiloxane has been spotlighted in pressure-sensitive adhesive (PSA) application due to excellent physical properties and good biocompatibility. Thermal behaviour of polyalkylsiloxane mixtures, such as thermal stability and heat flow, were studied using TG-DTA during catalytic hydrosilation. To understand reaction kinetics of cross-linking, catalytic hydrosilation of polyalkylsiloxane was monitored using variable temperature nuclear magnetic resonance (VT-NMR) as increased temperature. The formation of cross-linking bond $Si-CH_2-CH_2-Si$ was directly observed using distortionless enhanced by polarization transfer (DEPT) technique. Successfully polyalkylsiloxane PSA samples exhibited excellent adhesion properties by cross-linking reaction.

Immunological Variations of Flagella Antigens in Bacillus thuringiensis serovar kurstaki Temperature-sensitive Mutants (Bacillus thuringiensis serovar kurstaki 돌연변이 균주의 편모항원의 변이성)

  • 이형환;이영주;이희무
    • Microbiology and Biotechnology Letters
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    • v.17 no.1
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    • pp.81-83
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    • 1989
  • The flagella antigenic variation of nine Bacillus thuringiensis serovar kurstaki temperature-sensitive mutants grown at the permissive temperature (3$0^{\circ}C$) was detected by a serological agglutination between H-antigen and antiserum. The flagella antigens were injected to rabbits to prepared their antisera, and then their homologous and heterologous titers of the antisera were measured. The homologous titers were ranged from 1:6,400 to 1:12,800, but the heterologous titers were very low. The H-antigen of the wild type strain was not agglutinated to 4 heterologous antisera, ts-U23 not to 7, ts-U3l not 5, ts-U32 not to 4, ts-U33 not to 7, ts-U7l not to 4, ts-U73 not to 6, ts-U74 not to 6, ts-U91 not to 4 and ts-U603 not to 4 antisera.

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An Experimental Investigation of Unsteady Mixed Convection in a Horizontal Channel with Cavity Using Thermo-Sensitive Liquid Crystals

  • Bae, Dae-Seok;Cai, Long-Ji;Kim, Eun-Pil
    • Journal of Advanced Marine Engineering and Technology
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    • v.33 no.7
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    • pp.987-993
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    • 2009
  • An experimental study is performed to investigate unsteady mixed convection in a horizontal channel with a heat source. Particle image velocimetry (PIV) with thermo-sensitive liquid crystal (TLC) tracers is used for visualization and analysis. This method allows simultaneous measurement of velocity and temperature fields at a given instant of time. Quantitative data of the temperature and velocity are obtained by applying the color-image processing to a visualized image, and neural network is applied to the color-to-temperature calibration. It is found that the periodic flow of mixed convection in a cavity appears at very low Reynolds numbers (Re<0.4), and the period decreases with increasing Reynolds numbers and increases with increasing aspect ratio.

Development of finite element model using incremental endochronic theory for temperature sensitive material

  • Kerh, Tienfuan;Lin, Y.C.
    • Structural Engineering and Mechanics
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    • v.16 no.2
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    • pp.115-126
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    • 2003
  • A novel finite element model based on the incremental endochronic theory with the effect of temperature was developed in this study to explore the deformed behaviors of a flexible pavement material. Three mesh systems and two loading steps were used in the calculation process for a specimen of three-dimensional circular cylinder. Computational results in the case of an uni-axial compression test for temperatures at $20^{\circ}C$ and at $40^{\circ}C$ were compared with available experimental measurements to verify the ability of developing numerical scheme. The isotropic response and the deviatoric response due to the thermal effect were presented from deformations in different profiles and displacement plots for the entire specimen. The characteristics of changing asphalt concrete material under a specified loading condition might be seen clearly from the numerical results, and might provide an useful information in the field of road engineering.