• Proceedings of the Korean Vacuum Society Conference
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• 2011.08a
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• pp.192-192
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• 2011

Hyperthermal ion scattering experiments were conducted with low kinetic energy (<35 eV) cesium ion beams to analyze the UV-photolyzed water-ice films. Neutral molecules (X) on the surface were detected as cesium-molecule ion clusters ($CsX^+$) which were formed through a Reactive Ion Scattering (RIS) process. Ionic species on the surface were desorbed from the surface via a low energy sputtering (LES) process, and were analyzed [1]. Using these methods, the thermal stability of hydronium ion ($H_3O^+$) that was produced by UV light was examined. As the thermal stability of $H_3O^+$ is related with the reaction, $H_3O^+$ + OH + $e^-$ (or $OH^-$) ${\rightarrow}$ $2H_2O$, which is similar or same with the reverse reaction of the auto-ionization of water, the result from this work would be helpful to understand the auto-ionization of $H_2O$ in water-ice that has not been well-understood yet. However, as $H_3O^+$ was not detected through a LES method, the titration experiment of $H_3O^+$ with methylamine ($CH_3NH_2$, MA), MA + $H_3O^+\;{\rightarrow}\;MAH^+$ + $H_2O$, was conducted. In this case, the presence of $MAH^+$ indicates that of $H_3O^+$ in the ice. Thus the pristine ice was photolyzed with UV light for a few minutes and this photolyzed ice was remained at the certain temperature for minutes without UV light. Then MA was adsorbed on that surface so that the population of $H_3O^+$ was found. From the calibration experiments, the relation of $MAH^+$ and $H_3O^+$ was found, so that the thermal stability of $H_3O^+$ can be investigated [2].

• Analytical Science and Technology
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• v.8 no.4
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• pp.611-615
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• 1995

The design of appropriate chemically modified electrodes should allow development of new voltammetric measurement schemes with enhanced selectivity and sensitivity. Microorganism like algae has high ability to trap toxic and heavy metal ions and different affinities for metal ions. A copper(II) ion-selective carbon paste electrode was constructed by incorporating alga Anabaena into a conventional carbon paste mixture, and then the film of 10% Nafion was coated to avoid the swelling of the electrode surface. Copper ion could be deposited at the 25% algamodified electrode for 15 min without the applied potential while stirring the solution by only immersing the electrode in a buffer (pH 4.0) cot1taining copper(II). Temperature was controlled at $35^{\circ}C$. After preconcentration was carried out the electrode was transferred to a 0.1 M potassium chloride solution and was reduced at -0.6 volt at $25^{\circ}C$. The differential pulse anodic stripping voltammetry was employed. A well-defined oxidation peak could be obtained at -0.1 volt (vs SCE). In five deposition / measurement / regeneration cycles, the responses were reproducible and relative standard deviations were 3.3% for $8.0{\times}10^{-4}M$ copper(II). Calibration curve for copper was linear over the range from $2.0{\times}10^{-4}M$ to $1.0{\times}10^{-3}M$. The detection limit was $7.5{\times}10^{-5}M$. Studies of the effect of diverse ions showed that the coexisting metal ions had little or no effect for the determination of copper. But anions such as cyanide. oxalate and EDTA seriously interfered.

• Applied Biological Chemistry
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• v.18 no.1
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• pp.52-55
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• 1975

A microdetermination of Ascorbic acid (AsA) in blood by Gas-Liquid Chromatography (GLC) was studied. AsA was applied on GLC after the conversion into trimethylsilyl derivative (TMS) and the GLC was available for the only reduced form of AsA. A calibration curve is made by GLC of TMS-AsA as the internal standard of n-docosane. The minimum amount of AsA required for the determination was 0.5ml of 1 mg% pyridine solution. Prior to the conversion of AsA in serum into TMS derivative, serum was lyophilized and then it was allowed to stand at room temperature with TMS reagents for 48 hr. On injection of the supernates of TMS derivative to GLC the peak corresponding to AsA was not detected. Its reason why the concentration of AsA in serum is $0.5mg{\sim}0.8mg%$ in general, and it is less than minimum concentration of GLC. In case of L-AsA 1 mg was added to 1ml of serum, which was followed by lyophilization, silylation and GLC. The recorvery of AsA added was 98 percentage.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 2005.10a
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• pp.241-246
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• 2005

Doam watershed is located at alpine areas in the Kangwon province. The annual average precipitation, including snow accumulation during the winter, at the Doam watershed is significantly higher than other areas. Thus, pollutant laden runoff and sediment discharge from the alpine agricultural fields are causing water quality degradation at the Doam watershed. To estimate soil erosion from the agricultural fields, the Universal Soil Loss Equation (USLE) has been widely used because of its simplicity to use. The USLE rainfall erosivity (R) factor is responsible for impacts of rainfall on soil erosion. Thus, use of constant R factor for the Doam watershed cannot reflect variations in precipitation patterns, consequently soil erosion estimation. In the early spring at the Doam watershed, the stream flow increases because of snow melt, which results in erosion of loosened soil experiencing freezing and thaw during the winter. However, the USLE model cannot consider the impacts on soil erosion of freezing and thaw of the soil. Also, it cannot simulate temporal changes in USLE input parameters. Thus, the Soil and Water Assessment Tool (SWAT) model was investigated for its applicability to estimate soil erosion at the Doam watershed, instead of the widely used USLE model. The SWAT hydrology and erosion/sediment components were validated after calibration of the hydrologic component. The $R^2$ and Nash-Sutcliffe coefficient values are higher enough, thus it was found the SWAT model can be efficiently used to simulate hydrology and sediment yield at the Doam watershed. The effects of snow melt on SWAT estimated stream flow and sediment were investigated using long-term precipitation and temperature data at the Doam watershed. It was found significant amount of flow and sediment in the spring are contributed by melting snow accumulated during the winter. Thus, it is recommend that the SWAT model capable of simulating snow melt and long-term weather data needs to be used in estimating soil erosion at alpine agricultural land instead of the USLE model for successful soil erosion management at the Doam watershed.

• Korean Journal of Environmental Agriculture
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• v.31 no.2
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• pp.113-121
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• 2012

BACKGROUND: The Doam watershed in Korea has been managed for the reduction and the prevention of non-point source pollution since 2007. Especially, the water quality of the Doam reservoir is a primary issue related to the Doam dam reoperation. We have carried out the modeling to evaluate the water quality based on suspended solids (SS) of the Doam watershed and the Doam reservoir. Two powerful hydrological and water quality models (HSPF and CE-QUAL-W2) were employed to simulate the combined processes of water quantity and quality both in the upland watershed of the Doam reservoir and the downstream waterbody. METHODS AND RESULTS: The HSPF model was calibrated and validated for streamflow and SS. The CE-QUAL-W2 was calibrated for water level, water temperature, and SS and was validated for the only water level owing to data lack. With the parameters obtained through the appropriate calibration, SS concentrations of inflow into and in the Doam reservoir were simulated for three years (2008, 2004 and 1998) of the minimum, the average, and the maximum of total annual precipitation during recent 30 years. The annual average SS concentrations of the inflow for 2008, 2004, and 1998 were 8.6, 10.9, and 18.4 mg/L, respectively and those in the Doam reservoir were 9.2, 13.8, and 21.5 mg/L. CONCLOUSION(s): The results showed that more intense and frequent precipitation would cause higher SS concentration and longer SS's retention in the reservoir. The HSPF and the CE-QUAL-W2 models could represent reasonably the SS from the Doam watershed and in the Doam reservoir.

• YAKHAK HOEJI
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• v.60 no.2
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• pp.64-72
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• 2016

Jakyakgamcho-tang is a well-known traditional herbal medicine and has been used for the treatment of mainly pains in oriental medicine. In this study, analytical method for the quantitative determination of the eleven marker components, gallic acid (1), oxypaeoniflorin (2), paeoniflorin (3), albiflorin (4), liquiritin (5), isoliquiritin (6), ononin (7), liquiritigenin (8), benzoylpaeoniflorin (9), paeonol (10), and glycyrrhizin (11) in Jakyakgamcho-tang decoction was performed using an ultra-performance liquid chromatography-electrospray ionization-mass spectrometer. The analytical column for separation of the compounds 1~11 was used an UPLC BEH $C_{18}$ ($100{\times}2.1mm$, $1.7{\mu}m$) column and column oven temperature was maintained at $45^{\circ}C$. The mobile phase consisted of 0.1% (v/v) aqueous formic acid (A) and acetonitrile (B) by gradient elution. The flow rate was 0.3 ml/min and injection volume was $2.0{\mu}l$. Correlation coefficient in the calibration curves of the compounds 1~11 were showed a good linearity with more than 0.99. The limit of detection and limit of quantification values of the compounds 1~13 were detected in the ranges 0.06~18.43 ng/ml and 0.18~58.29 ng/ml, respectively. Among the compounds 1~11, the compounds 10 were not detected in this sample, while the ten compounds, 1~9 and 11, were detected $44.05{\sim}19,289.05{\mu}g/g$ in Jakyakgamcho-tang extract.

• YAKHAK HOEJI
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• v.54 no.3
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• pp.157-163
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• 2010

A high-performance liquid chromatography (HPLC) with DAD detector and electrospray ionization mass spectrometry (ESI-MS) was established for the simultaneous determination of coniferin (1), loganic acid (2), demethylsecologanol (3), sweroside (4) and loganin (5) from caulis Lonicera joponica. The optimal chromatographic conditions were obtained on an ODS column ($5{\mu}m$, $4.6{\times}150mm$) with the column temperature $35^{\circ}C$. The mobile phase was composed of (A) water with 0.1% formic acid and (B) methanol with 0.1% formic acid using a gradient elution, the flow rate was 0.3 ml/min. Detection wavelength was set at 254 nm. All calibration curves showed good linear regression ($r^2$>0.998) within test ranges. The developed method provided satisfactory precision and accuracy with overall intra-day and interday variations of 0.16~3.28% and 0.14~1.99%, respectively, and the overall recoveries of 99.39~105.89% for the five compounds analyzed. The verified method was successfully applied to quantitative determination of the two types (phenolic compounds and iridoids) of bioactive compounds in 24 commercial caulis L. japonica samples from different markets in Korea and China. The analytical results demonstrated that the contents of the five analytes vary significantly with sources.

• YAKHAK HOEJI
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• v.55 no.1
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• pp.64-68
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• 2011

In this study, a high performance liquid chromatography-diode array detector method was established, for simultaneous determination of three compounds, berberine, palmatine and geniposide in Hwangryunhaedok-tang, To develop and validate method, $C_{18}$ column (5 ${\mu}M$, 4.6 mm${\times}$250 mm) was used with gradient mobile phase, water containing 0.1% trifluoroacetic acid (TFA) and MeOH at the column temperature of $30^{\circ}C$. UV wavelength was set at 230 and 280 nm. Validation of the chromatography method was evaluated by linearity, precision and accuracy test. Calibration curve of standard components showed good linearity ($R^2$ > 0.9999). The limits of detection (LOD) and limits of quantification (LOQ) varied from 0.05 to 0.17 ${\mu}g/ml$ and 0.15 to 0.53 ${\mu}g/ml$, respectively. The relative standard deviations (RSDs) data of intra-day and inter-day test were in less than 2.99% and 1.90%, respectively. The results of the accuracy test were in the range of 98.36 to 102.52% with RSDs values 0.32 to 1.98%. The results of validation indicated that this method was a very accurate and sensitive assay.

• Journal of the Korean Chemical Society
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• v.45 no.3
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• pp.223-229
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• 2001

A method for the determination of glucose in human whole blood by chemiluminescence method using a stopped flow injection system has been studied. The method is based on the differences in the chemiluminescence intensities of luminol due to the different amounts of hydrogen peroxide produced from the glucose oxidase catalyzed reaction. The enzyme reactor was prepared by immobilization of glucose oxidase on aminopropyl glass beads and the chemiluminescence from a flow cell was measured by means of an optical fiber bundle. In order to obtain the optimum experimental conditions, effects of pH for the chemiluminogenic solution and enzyme reactor, flow rate and temperature on the chemiluminescence intensity were investigated. The calibration curve obtained under optimum experimental conditions was linear over the range from $1.0{\times}10^{-1}$ mM to 7.0 mM and the detection limit was $6.0{\times}10^{-2}$ mM. The proposed method was applied to the determination of glucose in whole human blood sample and the results were compared with those obtained by an official method. The present method was also evaluated by the results of recovery experiments.

• Journal of the Korean Magnetics Society
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• v.11 no.1
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• pp.38-44
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• 2001

The magnetic field standard below 1 mT with the resolution of 0.26 nT has been established. Earth magnetic field (EMF) is compensated automatically down to 0.1 nT/10 min. by a closed feedback system with Cs optical pumping magnetometer and 3-axis Helmholtz coils in nonmagnetic facilities. A multi-layer precision solenoid with the optimized single-current method generates the uniform magnetic field better than 1.0$\times$10$\^$-7/ within $\pm$ 1 cm region at its center. The coil constant of solenoid determined from Helium optical pumping magnetometer is 1.231 058 9 mT/A, and temperature coefficient is 0.38 nT/$\^{C}$. This standard system is used for calibration of low field magnetometers and testing relates to low field.