• Development and Reproduction
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• v.14 no.4
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• pp.307-316
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• 2010

This thesis attempts to describe the basis and value system respectively selected by opinions pro and against bio engineering technology and to induce discussions on the basis of the concept called 'two culture' issue by C. P. Snow - science culture, human culture, a teleological ethical theory and a deontological ethical theory, traditional eugenics, and liberalism eugenics. In addition, it will discuss about inconvenient truth about science and bio engineering which we have wrongly understood up to now - a myth on objectivity of science, autonomy of science and a scientist, a hunger of world, science expected to be responsible for all men. Lastly, it will describe study ethics and social responsibility, openness, moral imagination, self-examination, character of a bio engineer as professionalism.

• Journal of Microbiology and Biotechnology
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• v.32 no.3
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• pp.341-347
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• 2022

In this study, the bacterial community of galchi-baechu kimchi was determined using culture-based and culture-independent techniques (next generation sequencing:NGS), and showed discrepancies between results. Weissella koreensis and Pediococcus inopinatus were the dominant species according to the NGS results, while Bacillus species and P. inopinatus were dominant in the culture-dependent analysis. To identify safe starter candidates, sixty-five Bacillus strains isolated from galchi-baechu kimchi using culture-dependent methods were evaluated for their antibiotic resistance, presence of toxin genes, and hemolytic activity. Strains were then assessed for salt tolerance and protease and lipase activity. As a result, four strains-B. safensis GN5_10, B. subtilis GN5_19, B. velezensis GN5_25, and B. velezensis GT8-were selected as safe starter candidates for use in fermented foods.

• Journal of international Conference on Electrical Machines and Systems
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• v.1 no.3
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• pp.321-329
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• 2012

Providing variable load for testing a motor in high speed conditions is usually a difficult task. The eddy current brake can be used in application of load testing of motors. This paper deals with construction of a novel claw pole eddy current brake which is employed as a load for a DC counter rotating motor (CRM). These kinds of motors have two inner and outer shafts that rotate in opposite directions simultaneously, which are particularly suitable for under water propulsion systems. The prototype 45KW eddy current brake consists of two parts. One of them is installed on the inner shaft of the 60KW DC CRM and the other one is installed on its outer shaft. The simulation and experimental results with prototype brakes are also analyzed by using MATLAB/Simulink and the operational characteristic of the brake is demonstrated as a function of the motor speed and current of the magnetic poles.

• Elastomers and Composites
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• v.50 no.3
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• pp.189-195
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• 2015

In this study, we prepared epoxidized poly ethylene-ter-1-decene-ter-divinylbenzene (Epo-PEHV) as a reactive compatibilizer to prevent phase separation phenomenon which occurs upon blending polypropylene (PP) and ethylene-vinyl alcohol copolymer (EVOH). Firstly, PEHV was prepared under high catalyst activity according to content of catalyst and cocatalyst. After then, we modified vinyl group of the terpolymer with epoxy group. We observed that the terpolymer was successfully epoxidized by 1H-NMR and FT-IR analysis. The Epo-PEHV was added by 2, 5, 10% in PP/EVOH blends. The morphologies and mechanical properties of PP/Epo-PEHV/EVOH blends were analyzed by SEM and UTM, respectively. Epo-PEHV enhanced the interfacial adhesion of PP and EVOH blends.

• The Journal of Information Technology
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• v.12 no.1
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• pp.29-34
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• 2009

Culture contents are in our life with various parts. There are much try and research managing and keeping the culture contents with growing of information technique. Digital archive was proposed the technique that is able to distribute and manage the contents. This paper proposed the web based digital archive for culture contents. Each of archive were sorted art, architecture, science technology and etc, and made the structure that can total manage.

• Journal of Veterinary Science
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• v.21 no.1
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• pp.13.1-13.14
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• 2020

Currently, the systems for culturing buffalo spermatogonial stem cells (SSCs) in vitro are varied, and their effects are still inconclusive. In this study, we compared the effects of culture systems with undefined (foetal bovine serum) and defined (KnockOut Serum Replacement) materials on the in vitro culture of buffalo SSC-like cells. Significantly more DDX4- and UCHL1-positive cells (cultured for 2 days at passage 2) were observed in the defined materials culture system than in the undefined materials system (p < 0.01), and these cells were maintained for a longer period than those in the culture system with undefined materials (10 days vs. 6 days). Furthermore, NANOS2 (p < 0.05), DDX4 (p < 0.01) and UCHL1 (p < 0.05) were expressed at significantly higher levels in the culture system with defined materials than in that with undefined materials. Induction with retinoic acid was used to verify that the cultured cells maintained SSC characteristics, revealing an SCP3+ subset in the cells cultured in the defined materials system. The expression levels of Stra8 (p < 0.05) and Rec8 (p < 0.01) were significantly increased, and the expression levels of ZBTB16 (p < 0.01) and DDX4 (p < 0.05) were significantly decreased. These findings provided a clearer research platform for exploring the mechanism of buffalo SSCs in vitro.

• Journal of Animal Science and Technology
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• v.47 no.4
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• pp.547-554
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• 2005

An experiment was conducted to investigate the dietary effects of a transgenic Aspergillus oryzae(AO) culture on the performance, egg quality and intestinal microflora of layers. A total of 840 Hy-line Brown layers of 39wks old were assigned to one of the following 7 dietary treatments: control(C), C+0.2% AO culture, C+0.5% AO culture, C+0.2% transgenic AO culture, C+0.5% transgenic AO culture, C+0.2% transgenic mutant AO culture, and C+0.5% transgenic mutant AO culture. The transgenic AO was made by inserting Salmonella gallinarum gene to AO. And the transgenic mutant AO was made by inserting Salmonella gallinarum gene to mutant AO which was mutated by UV irradiation. Each treatment was replicated six times with 20 birds housed in 2 bird cage. Twenty birds units were arranged according to completely randomized block design. Feeding trial lasted for 8wks under 16 hour lighting regimen. Laying performance and egg quality were significantly(P<0.05) affected by the treatments. Transgenic AO culture supplementation at the level of 0.2% significantly increased egg production, while its egg weight was significantly decreased compared to that of the control. Feed intake and feed conversion ratio(FCR) were not significantly different among the AO treatments and the control. The eggshell strength of the AO treatments was significantly higher than that of the control. Transgenic mutant AO culture supplemented at the level of 0.5% significantly increased egg yolk color. Intestinal microflora were significantly(P<0.05) affected by the treatments. The cfu of Lactobacilli spp. significantly increased and those of Salmonella species and E. coli decreased in the AO treatments. The transgenic AO and transgenic mutant AO culture were more effective than the AO culture in reducing the cfu of Salmonella species and E. coli. It is concluded that supplementation of the transgenic AO culture at the level of 0.2% could be recommended for the improvement of egg production. Supplementation of transgenic AO or transgenic mutant AO culture at 0.2% level effectively controlled intestinal Salmonella species population.

• Plant Biotechnology Reports
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• v.2 no.1
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• pp.1-6
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• 2008

Cotyledonary leaves of tomato cv. Megha were transformed with the hepatitis B virus 's' gene, which encodes surface antigen. Six plant expression cassettes (pHBS, pHER, pEFEHBS, pEFEHER, pSHER and pEFESHER) were used to assay the possible expression levels by agroinfiltration. The maximum transient expression level of 489.5 ng/g D.W. was noted in pEFEHER-infiltrated cotyledonary leaves. Transgenic tomato plants with pEFEHBS and pEFEHER expression cassettes were regenerated and characterized by molecular analysis. The expression of the antigen in the fruits was confirmed by RT-PCR and ELISA analysis. This is the first report on the expression of hepatitis B surface antigen in tomato.

• Knowledge Management Research
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• v.6 no.2
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• pp.47-68
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• 2005

The Purpose of this study is to identify relationships between knowledge management performances and such Influence factors of knowledge Management as information technology, knowledge management process, culture & people, strategy & Leadership in Small and Medium-sized Enterprises. In order to test hypotheses, we collected data from 498 SME's employees, then analysed them by SPSSWIN. This results showed as follows; 1. All Influence factors of knowledge Management( information technology, knowledge management process, culture & people, strategy & Leadership) are to advance individual task effectiveness and organization effectiveness significantly. 2. Such Influence factors of knowledge Management as information technology, knowledge management process, culture & people are to improve business efficiency between the company. But strategy & leadership factor is not significant. 3. Influence factors of knowledge Management are different between knowledge performace factors such ac individual task effectiveness, organization effectiveness, and business efficiency between company.

• Elastomers and Composites
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• v.51 no.3
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• pp.188-194
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• 2016

In this study, novel quaternary plastomers consisting of ethylene, 1-hexene, high ${\alpha}$-olefin, and divinylbenzene were prepared using Zr metallocene catalyst, borate type cocatalyst, and tri-isobutylaluminium. The molar ratio changes of 1-hexene and high ${\alpha}$-olefin (1-octene, 1-decene, and 1-dodecene) had an effect on the properties of the quaternary plastomers. The structure of the quaternary plastomers was characterized using $^1H$ NMR. Molecular weight properties, crystallinity, and thermal properties of the plastomers were determined by GPC, WAXS, and DMA, respectively. Compared with the terpolymers prepared in our previous study, molecular weight and molecular weight distribution of the quaternary polymers were very similar, whereas glass transition temperature ($T_g$) was very low. Also, hardness and tensile properties of the quaternary plastomers were measured.