• Archives of Pharmacal Research
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• 제26권3호
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• pp.214-223
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• 2003

The present study was conducted to investigate the effects of green tea extract (GTE) on arterial blood pressure and contractile responses of isolated aortic strips of the normotensive rats and to establish the mechanism of action. The phenylephrine ($10^{-6}~10^{-5}M$)-induced contractile responses were greatly inhibited in the presence of GTE (0.3~1.2 mg/mL) in a dose-dependent fashion. Also, high potassium ($3.5{\times}10^{-2}~5.6{\times}10^{-2}{\;}M$)-induced contractile responses were depressed in the presence of 0.6~1.2 mg/mL of GTE, but not affected in low concentration of GTE (0.3 mg/mL). However, epigallocatechin gallate (EGCG, $4~12{\;}{\mu}g/mL$) did not affect the contractile responses evoked by phenylephrine and high $K^+$. GTE (5~20 mg/kg) given into a femoral vein of the normotensive rat produced a dose-dependent depressor response, which is transient. Interestingly, the infusion of a moderate dose of GTE (10 mg/kg/30 min) made a significant reduction in pressor responses induced by intravenous norepinephrine. However, EGCG (1 mg/kg/30 min) did not affect them. Collectively, these results obtained from the present study demonstrate that intravenous GTE causes a dose-dependent depressor action in the anesthetized rat at least partly through the blockade of adrenergic $\alpha_1$-receptors. GTE also causes the relaxation in the isolated aortic strips of the rat via the blockade of adrenergic $\alpha_1$-receptors, in addition to the unknown direct mechanism. It seems that there is a big difference in the vascular effect between GTE and EGCG.

• 대한가정학회지
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• 제46권3호
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• pp.49-55
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• 2008

The purpose of this study was to investigate the effects of green tea extracts (GTE) on the fermentation properties of polyphenol-enriched beers. As such, the formation patterns of tannoid in beer with GTE were investigated at 3 different infusion times, while the ale and the lager beers fortified with GTE were analyze to ascertain effects on gravity, pH, yeast viability, total polyphenol, and tannoid during fermentation period. The results were as follows: 1) The formation of tannoid in beer with GTE in the tannometer; In reaction of GTE with polyvinylpyrrolidon (PVP), control lager beer peaked in the formation of tannoid at $70\;{\sim}\;80\;mg$ of PVP, the 1st extract exceeded the detection limit, the 2nd extract at $170\;{\sim}\;180\;mg$, while the third extract at $150\;{\sim}\;160\;mg$ of PVP. The GTE were slow in reacting with PVP, and their formation patterns were different from those of polyphenols from barley and hop. 2) Ale fermentation; The final alcohol content was 9.25% (ABV). The addition of GTE increased the yeast viability after 2 days and finally reached 52.3% from 30.9%. Total polyphenol in GTE beer increased by 1.5 times (p < .05). However, its tannoid contents increased by 6.4 times. 3) Lager fermentation; The final alcohol content was 5.93% (ABV). The effect of GTE on lager beer was minimal for all variables. However, total polyphenol of GTE beer increased by 1.4 times (p < .05). Its tannoid increased by 3.3 times (p < .05).

• The Korean Journal of Pain
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• 제22권3호
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• pp.199-205
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• 2009

Background: A major ingredient of green tea is epigallocatechin-3-gallate (EGCG), and this is known to have many beneficial effects for cancer prevention and also on the cardiovascular system and neurodegenerative diseases through its anti-oxidant, anti-angiogenic, anti-inflammatory, lipid-lowering and neuroprotective properties. Its actions on nociception and the spinal nervous system have been examined in only a few studies, and in these studies EGCG showed an antinociceptive effect on inflammatory and neuropathic pain, and a neuroprotective effect in motor neuron disease. This study was performed to investigate the effect of EGCG on acute thermal pain and the development of morphine tolerance at the spinal level. Methods: The experimental subjects were male Sprague-Dawley rats and the Hot-Box test was employed. A single or double-lumen intrathecal catheter was implanted at the lumbar enlargement for drug administration. An osmotic pump was used to infuse morphine for 7 days for induction of morphine tolerance. EGCG was injected repeatedly for 7 days at twice a day through the intrathecal catheter. Results: Intrathecal EGCG increased the paw withdrawal latency (PWL) after repeated administration for 7 days at twice a day, but this did not happen with administering on single bolus injection of EGCG. In addition, the antinociceptive effect of intrathecal morphine was not affected by co-administration with EGCG. A continuous 7-day infusion of morphine caused a significant decrease of the PWL in the control group (M + S, morphine plus saline). In contrast, intrathecal EGCG injection over 7 days blocked the decrease of the PWL in the experiment group (M + E, morphine plus EGCG). Conclusions: Intrathecal ECGC produced a weak antinociceptive effect for acute thermal pain, but it did not change the morphine's analgesic effect. However, the development of antinociceptive tolerance to morphine was attenuated by administering intrathecal EGCG.

• Asian Pacific Journal of Cancer Prevention
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• 제17권3호
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• pp.1453-1461
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• 2016

Regarding 'mate' intake (infusion of Ilex paraguariensis herb, a staple beverage in temperate South American regions), most epidemiologic studies showed positive associations with risk of some cancers, (e.g. upper aerodigestive tract), but evidence on breast cancer (BC) risk is limited to a previous multi-site study, which reported a non significant odds ratio [OR]=0.85, 95% confidence interval [95% CI] 0.67-1.09, p for trend=0.31) for the highest quartile of intake. The present study was conducted in order to further assess associations of 'mate' intake with BC risk. We combined two databases of women belonging to public and private healthcare hospitals. The sample included 572 BC incident cases and 889 controls interviewed with a specific questionnaire featured by socio-demographic, reproductive and lifestyle variables, and a food frequency questionnaire of 64 items, also analyzing 'mate' intake (consumer status, daily intake, age at start, age at quit, duration of habit, intensity of intake). ORs and their 95%CI were calculated through unconditional logistic regression, adjusting for relevant potential confounders. The highest quartile of 'mate' intake was inversely associated with BC risk (OR=0.40, 95%CI 0.26-0.57, p for trend <0.001). Stratified analyses also displayed strong significant inverse associations for 'mate' in frequent tea drinkers (OR=0.22), high energy intake (OR=0.23), high body mass index (OR=0.29) and in postmenopausal women (OR=0.36), among other results. As conclusions, we found evidence of a significant inverse association for 'mate' intake and BC risk.

• 한국식품영양과학회지
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• 제41권1호
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• pp.73-78
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• 2012

트랜스 지방 과다섭취는 심혈관계 질환의 위험인자이다. 이 연구는 녹차의 섭취가 대표적인 트랜스 지방인 elaidic acid의 소장 흡수율에 미치는 영향을 조사하기 위해서 설계하였다. 소장 지방 흡수를 in vivo 상태에서 측정하기 위해서 흰쥐의 '소장지방흡수모델(mesenteric lymph duct cannulated rat model)'을 이용하여 십이지장주입관(intraduodenal catether)과 림프채취관(lymph duct cannula)을 각각 십이지장과 소장 림프관에 설치하였다. 십이지장주입관으로 주입된 지질유화액은 $180.0{\mu}mol$ elaidic acid, $400.0{\mu}mol$ triolein, $20.7{\mu}mol$ cholesterol, $3.1{\mu}mol$ a-tocopherol, $396{\mu}mol$ sodium taurocholate 그리고 24 mL PBS 용액을 기본성분으로 했고 이 지질유화액만 공급받은 동물을 대조군(control), 녹차추출물이 추가된 지질유화액을 공급받은 동물을 녹차군으로 하였다. 지질유화액은 시간당 3 mL씩 8시간 동안 주입하였고 동시에 소장 림프관에 설치된 림프채취관으로 분비되는 림프 시료를 매시간 8시간 동안 채취하여 분석하였다. 결과적으로, 8시간 동안 소장 림프채취관으로 분비된 림프의 양은 녹차에 의해서 유의적으로 감소하였고 elaidic acid의 흡수율(분비율) 또한 녹차에 의해서 유의적으로 감소하였다. 콜레스테롤, 올레산, 인지질의 흡수율도 녹차에 의해서 유의적으로 감소하였다. 이상의 결과들은 녹차가 elaidic acid 뿐만 아니라 기타 주요 식이성 지방들의 소장흡수를 억제하는데 효과적인 수단이 될 수 있다는 것을 증명한 연구라 판단된다.

• 셀메드
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• 제2권1호
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• pp.11.1-11.7
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• 2012

Stachys tibetica Vatke (Lamiaceae) is an important medicinal plant in the folk medicine of Ladakh, India and Tibet for the treatment of various mental disorders. Infusion and decoction of the whole plant is used as a cup of tea for a severe fever, headaches and to relieve tension. The recent study is aimed to evaluate the preliminary pharmacognostical and phytochemical nature of Stachys tibetica Vatke. The whole plant material was subjected to successive soxhlet extraction with petroleum ether (40 - $60^{\circ}C$), chloroform, ethyl acetate, methanol and finally decocted with water to get the respective extracts. The fluorescence characteristics of the powdered materials were analysed under ultraviolet light and ordinary light. Different physicochemical parameters such as ash value, extractive value, foaming index, pH values, loss on drying and determination of foreign matter were carried out as per WHO guidelines. The total fat, flavonoid, saponin and volatile contents were also determined. Macroscopical studies revealed the authentication of the plant drug. Physicochemical parameters helped to standardize the plant material while preliminary qualitative chemical tests of different extracts showed the presence of Glycosides, Carbohydrates, Phytosterols/triterpenoids, Saponins, Fixed oils, Fats and phenols/tannins. Quantification of the total flavonoids and saponins and contents were determined as $54.66{\pm}0.58mg/g$ and $75.42{\pm}0.48mg/kg$ respectively, while the volatile and fat contents were 6.5% and 0.7% respectively. Results may lay the foundation for the standardization of the drug and discovery of new molecules from S. tibetica for the treatment of various diseases.

• 대한임상독성학회지
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• 제17권1호
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• pp.42-45
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• 2019

Dioscorea tokoro has long been used in Korean traditional medicine as a pain killer and anti-inflammatory agent. A 53-year-old male who consumed water that had been boiled with raw tubers of D. tokoro as tea presented with numbness and spasm of both hands and feet. Laboratory results showed hypocalcemia, hypoparathyroidism, and vitamin D insufficiency. During his hospital stay, colitis, acute kidney injury, and toxic encephalopathy developed. The patient received calcium gluconate intravenous infusion and oral calcium carbonate with alfacalcidol. His symptoms improved gradually, but hypocalcemia persisted despite the calcium supplementation. We suggest that ingestion of inappropriately prepared D. tokoro can cause symptomatic hypocalcemia in patients with unbalanced calcium homeostasis.

• Journal of Animal Science and Technology
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• 제64권3호
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• pp.397-408
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• 2022

Rinse & Chill^® technology (RCT) entails rinsing the vasculature using a chilled isotonic solution (3℃; 98.5% water and a blend of dextrose, maltose, and sodium phosphates) to rinse out the residual blood from the carcass. Infusion of pre-chilled solutions into intact animal carcasses immediately upon exsanguination is advantageous in terms of lowering the internal muscle temperature and accelerating chilling. This technology is primarily used for purposes of effective blood removal, favorable pH decline, and efficient carcass chilling, all of which improve meat quality and safety. Although RCT solution contains some substrates, the pre-rigor muscle is still physiologically active at the time of early postmortem and vascular rinsing. Consequently, these substrates are fully metabolized by the muscle, leaving no detectable residues in meat. The technology has been commercially approved and in continuous use since 2000 in the United States and since 1997 in Australia. As of January 2022, 23 plants have implemented RCT among the 5 countries (Australia, US, Canada, New Zealand, and Japan) that have evaluated and approved RCT. All plants are operating under sound Sanitation Standard Operation Procedures (SSOP) and a sound Hazard Analysis Critical Control Point (HACCP) program. No food safety issues have been reported associated with the use of this technology. RCT has been adapted by the meat industry to improve product safety and meat quality while improving economic performance. Therefore, this review summarizes highlights of how RCT technically works on a variety of animal types (beef, bison, pork, and lamb).

• Childhood Kidney Diseases
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• 제16권2호
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• pp.89-94
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• 2012

목적: 소아 입원 환자에서 유지용액을 기존의 저장성 수액 대신 등장성 수액으로 대체해야 된다는 주장이 제기되어왔다. 소아에서의 폐렴과 중추신경계 감염은 항이뇨 호르몬의 분비 자극으로 인해 저나트륨혈증이 흔히 발생할 수 있는 임상적인 상황으로 알려져 있다. 이에 저자들은 소아에서 폐렴과 중추신경계 감염질환에서 저나트륨혈증의 발생률을 확인하고 유지용액 선택의 중요성과 관련 인자들에 대해 조사하고자 본 연구를 시행하게 되었다. 방법: 부산대학교 어린이병원에 2008년 11월부터 2011년 8월까지 폐렴과 중추신경계 감염질환진단하에 입원하여 유지용액을 투여받고 혈장 나트륨 농도가 측정된 1992명의 환아를 대상으로 하였다. 저나트륨혈증은 혈장 나트륨 농도가 135 mEq/L 미만일 때로 정의하였다. 검사결과를 포함한 임상적 자료는 후향적으로 분석되었다. 결과: 연구기간 동안 1,992명 중 218명의 환아가 급성 저나트륨혈증으로 확인되었다. 따라서 총 발생률은 10.9%였다. 저나트륨혈증의 발생률은 뇌염에서 가장 높았고 세균성 뇌수막염, 바이러스성 뇌수막염, 세균성 폐렴, mycoplasma성 폐렴, 바이러스성 폐렴 순으로 발생률의 차이를 나타내었다. 세균성 폐렴에서의 발생률은 바이러스성 폐렴의 발생률에 비해 의미있게 높은 결과를 보였다. 또한 뇌염에서의 발생률은 바이러스성 뇌수막염의 발생률에 비해 의미있게 높은 결과를 보였다. 평균발생연령은 저나트륨혈증 환아군이 정상나트륨혈증 환아군에 비해 의미있게 높았다. 저나트륨혈증의 발생률은 D5 0.18% NS 투여군이 D5 1/2NS 투여군에 비해 의미있게 높았다. 입원기간 중 저나트륨혈증이 발생한 82명의 환아 중 78명이 SIADH의 진단에 필요한 모든 검사를 시행하였고, 이중 20.5%에서 진단되었다. 결론: 저나트륨혈증의 발생률은 폐렴과 중추신경계 감염질환에서 높게 나타나며 저장성 수액의 투여는 입원 후에 발생하는 저나트륨혈증의 중요한 원인이다. 따라서 저자들은 폐렴과 중추신경계 질환이 있는 환아에서 유지용액으로 D5 0.18% NS 보다 D5 1/2NS의 투여를 권장하는 바이다.