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Comparison of Green Tea Extract and Epigallocatechin Gallate on Blood Pressure and Contractile Responses of Vascular Smooth Muscle of Rats  

Lim, Dong-Yoon (Department of Pharmacology, College of Medicine, Chosun University)
Lee, Eun-Sook (Department of Pharmacology, College of Medicine, Chosun University)
Park, Hyeon-Gyoon (Department of Pharmacology, College of Medicine, Chosun University)
Kim, Byeong-Cheol (Department of Pharmacology, College of Medicine, Chosun University)
Hong, Soon-Pyo (Department of Internal Medicine (Cardiology), College of Medicine, Chosun University)
Lee, Eun-Bang (Natural Products Research Institute, Seoul National University)
Publication Information
Archives of Pharmacal Research / v.26, no.3, 2003 , pp. 214-223 More about this Journal
Abstract
The present study was conducted to investigate the effects of green tea extract (GTE) on arterial blood pressure and contractile responses of isolated aortic strips of the normotensive rats and to establish the mechanism of action. The phenylephrine ($10^{-6}~10^{-5}M$)-induced contractile responses were greatly inhibited in the presence of GTE (0.3~1.2 mg/mL) in a dose-dependent fashion. Also, high potassium ($3.5{\times}10^{-2}~5.6{\times}10^{-2}{\;}M$)-induced contractile responses were depressed in the presence of 0.6~1.2 mg/mL of GTE, but not affected in low concentration of GTE (0.3 mg/mL). However, epigallocatechin gallate (EGCG, $4~12{\;}{\mu}g/mL$) did not affect the contractile responses evoked by phenylephrine and high $K^+$. GTE (5~20 mg/kg) given into a femoral vein of the normotensive rat produced a dose-dependent depressor response, which is transient. Interestingly, the infusion of a moderate dose of GTE (10 mg/kg/30 min) made a significant reduction in pressor responses induced by intravenous norepinephrine. However, EGCG (1 mg/kg/30 min) did not affect them. Collectively, these results obtained from the present study demonstrate that intravenous GTE causes a dose-dependent depressor action in the anesthetized rat at least partly through the blockade of adrenergic $\alpha_1$-receptors. GTE also causes the relaxation in the isolated aortic strips of the rat via the blockade of adrenergic $\alpha_1$-receptors, in addition to the unknown direct mechanism. It seems that there is a big difference in the vascular effect between GTE and EGCG.
Keywords
Green tea extract; Epigallocatechin gallate; Vasorelaxation; Adrenergic${\alpha}_1$-receptors blockade;
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