• Proceeding of EDISON Challenge
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• 2013.04a
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• pp.13-24
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• 2013

Dopamine agonists and antagonists and its receptor play a critical role in the information transfer in the nervous system, and dopamine receptor-ligands interactions are deeply related to Parkinson's disease, schizophrenia and some other mental diseases. However, the only experimental 3D structure available for dopamine receptors is human D3 dopamine receptor. Therefore, it is important to create model of subtype dopamine receptor-ligands interactions. We report here the 3D structures of the human D1 and D2 dopamine receptor predicted by using GalaxyTBM, and its predicted binding site determined by using GalaxyDock. The highly conserved Asp on TM 3 and Phe on TM 6 have critical role in ligand binding. Also, highly conserved serines on TM 5 are essential for binding agonists and some kinds of antagonists. We identify differences between binding sites of agonists and antagonists of human D1 and D2 dopamine receptor, and find the reasons of selective binding of antagonists.

• Proceeding of EDISON Challenge
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• 2016.03a
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• pp.86-89
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• 2016

The Zika virus, which is a member of the flavivirus genus, poses a serious threat to humanity because there is no vaccine or cure. Zika is suspected to cause microcephaly, and it is rapidly spreading throughout parts of Brazil. Surprisingly, there are no known protein structures for the virus which are essential for drug and vaccine development. This paper investigates the Zika virus's nonstructural proteins with template-based modeling by using GalaxyTBM/Refine/SC. GalaxyDock was used to examine the effectiveness of various known serine protease inhibitors in inhibiting the Zika viral protease. In testing five inhibitors, Kunitz soybean trypsin inhibitor showed the strongest binding affinity (-10.082 kcal/mol). This paper provides a rudimentary foundation for further drug discovery research regarding the Zika virus.

• Journal of the Korean Society of Safety
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• v.18 no.3
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• pp.54-59
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• 2003

The discharge energy by electrostatic discharge of the charged human body is calculated under the assumption that the stored charge is dissipated completely. However, it is well-known that the charge is slightly remained after electrostatic discharge. Therefore, The Rompe-Weisel model of the discharge analysis, which has somewhat more of a physical justification than the conventional energy equation, is proposed. It is proposed that the electrical conductivity of the arc should be proportional to the energy density transferred to it by Ohmic dissipation. For the electrostatic discharge energy analysis, the Rompe-Weisel model was compared by quasi static analysis. As a consequence, a study on a reliable energy evaluation based on simulation models during electrostatic discharge is carried out in this paper and is adopted to estimate the explosion hazards of flammable gases.

• Proceedings of the KSR Conference
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• 2008.11b
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• pp.235-240
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• 2008

Since urban railway transit is one of the most essential transportation systems, its power facilities must ensure high reliability and safety. Currently, urban railway operating organizations perform TBM (Time Based Maintenance) on power facilities. However, in order to improve management efficiency and system safety, CBM (Condition Based Maintenance) is preferred. Among various power facilities, mold transformers has been chosen as the object of study since it is widely used for the purpose of minimizing volume and weight, and due to safety against fire. In this paper, various transformer failure cases due to electric, thermal, mechanical and environmental factors have been collected and analyzed. In addition, investigation on national and international condition based maintenance cases and the characteristics of sensors widely used for transformer monitoring has been performed to suggest the optimal condition based maintenance technique for urban railway systems.

• Geomechanics and Engineering
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• v.29 no.3
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• pp.219-228
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• 2022

The most widely used parameter to represent rock abrasiveness is the Cerchar abrasivity index (CAI). The CAI value can be applied to predict wear in TBM cutters. It has been extensively demonstrated that the CAI is affected significantly by cementation degree, strength, and amount of abrasive minerals, i.e., the quartz content or equivalent quartz content in rocks. The relationship between the properties of rocks and the CAI is investigated in this study. A database comprising 223 observations that includes rock types, uniaxial compressive strengths, Brazilian tensile strengths, equivalent quartz contents, quartz contents, brittleness indices, and CAIs is constructed. A linear model is developed by selecting independent variables while considering multicollinearity after performing multiple regression analyses. Machine learning-based regression methods including support vector regression, regression tree regression, k-nearest neighbors regression, random forest regression, and artificial neural network regression are used in addition to multiple linear regression. The results of the random forest regression model show that it yields the best prediction performance.

• Journal of the Korean Society of Visualization
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• v.21 no.3
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• pp.33-38
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• 2023

Spray cooling is a method of cooling high-temperature heating elements by spraying droplets. Recently, spray cooling has been proposed for use in next-generation nuclear reactors. When droplets are sprayed onto the outer wall of a heat exchanger tube, a film boiling occurs on the outer wall. Over time, the outer wall temperature decreases, and a liquid film forms on the outer wall, and the heat exchanger outer wall is subsequently cooled by the liquid film. In this case, the liquid film thickness has a great influence on the heat removal performance. In this study, an experimental study was conducted to measure the liquid film thickness distribution in a droplet spray environment. For this purpose, a method using the electrical conductivity of the liquid was adopted.

• Structural Engineering and Mechanics
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• v.89 no.1
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• pp.47-59
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• 2024

In this study, a new sustainable material was proposed to prepare precast tunnel lining segments (TLS), which were produced using a fiber-reinforced slag-based geopolymer composite. Slag was used as the geopolymer binder. In addition, polypropylene and carbon fibers were added to reinforce TLSs. TLSs were examined in terms of flexural performance, load-deflection response, ductility, toughness, crack characteristics, and tunnel boring machine (TBM) thrust force. Simultaneously, numerical simulation was performed using finite element analysis. The mechanical characteristics of the geopolymer composite with a fiber content of 1% were used. The results demonstrated that the flexural performance and load-deflection response of the precast TLSs were satisfactory. Furthermore, the numerical results were capable of predicting and realistically capturing the structural behavior of precast TLSs. Therefore, fiber-reinforced slag-based geopolymer composites can be applied as precast TLSs.

• Korean Journal of Animal Reproduction
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• v.27 no.3
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• pp.215-223
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• 2003

This study was to investigate the effects of fertilization time and culture medium of pig oocytes matured in-vitro by liquid boar sperm. The sperm rich fraction (30∼60 ml) was slowly cooled to room temperature (20∼23$^{\circ}C$) by 2 h after collection. Semen was transferred into 15 ml tubes, centrifuged at room temperature for 10 min 800 ${\times}$ g, and the supernatant solution was poured off. The concentrated sperm was resuspended with 5 ml of the LEN diluent to provide 1.0${\times}$10$^{9}$ sperm/ml at room temperature. The resuspended semen was cooled in a refrigerator to 4$^{\circ}C$. The medium used for oocyte maturation was TCM-199 supplemented with 26.19 mM sodium bicarbonate, 0.9 mM sodium pyruvate, 10 $\mu\textrm{g}$/ml insulin, 2 $\mu\textrm{g}$/ml vitamin B$_{12}$ , 25 mM HEPES, 10 $\mu\textrm{g}$/ml bovine apotransferrin, 150 $\mu$M cysteamine, 10 IU/ml PMSG, 10 IU/ml hCG, 10 ng/ml EGF, 0.4% BSA, 75 $\mu\textrm{g}$/ml sodium penicillin G, 50 $\mu\textrm{g}$/ml streptomycin sulfate and 10% pFF. After about 22 h of culture, oocytes were cultured without cysteamine and hormones for 22 h at 38.5$^{\circ}C$, 5% $CO_2$ in air. Oocytes were inseminated with liquid boar sperm stored at 4$^{\circ}C$ for 2 days after collection. Oocytes were coincubated for 1, 3, 6 and 9 h in 500 ${mu}ell$ mTBM fertilization media with 1.0${\times}$10$^{6}$ sperm/ml concentration, respectively. Thereafter, oocytes were transferred into 500 ${mu}ell$ NCSU-23, HEPES buffered NCSU-23, PZM-3 and PZM-4 culture media, respectively, for further culture of 6, 48 and 144 h. The rates of sperm penetration and male pronuclear formation were higher in the fertilization times for 6 and 9 h than in those for 1 and 3 h. The rates of cleaved oocytes were higher in the fertilization times for 6 and 9 h (85.0 and 84.6%) than in those for 1 and 3 h (61.1 and 76.8%). The percentage of blastocyst formation from the cleaved oocytes was highest in the fertilization time for 6 h (33.6%) than in that for 1, 3 and 9 h (11.4, 23.0 and 29.6%). Mean cell numbers per blastocyst were 32.9, 27.6, 26.3 and 24.4 in the fertilization times for 6, 9, 3 and 1 h, respectively. The rate of blastocyst from the cleaved oocytes and the number of cells per blastocyst were higher in HEPES buffered NCSU-23 culture medium than in NCSU-23, PZM-3 and PZM-4 culture media. In conclusion, we found out that liquid boar sperm stored at 4$^{\circ}C$ could be used for in-vitro fertilization of pig oocytes matured in-vitro. Also, we recommend the coincubation time of 6 h in 500 ${mu}ell$ TBM fertilization medium with 1${\times}$10$^{6}$ sperm/ml concentration and the HEPES buffered NCSU-23 culture medium for in-vitro fertilization of pig oocytes matured in-vitro.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.32 no.4D
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• pp.407-419
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• 2012

It is difficult to obtain the accurate and homogeneous height information over the whole Korea due to the effect of different vertical datums have been divided into land and sea part. In this study, we tried to unify the different vertical datums using the precise spirit leveling between TBM (tidal bench mark) and BM (bench mark) in order to solve the problems caused by different vertical datums. For this, the vertical datum offsets at observed points which were calculate from leveling results and then transformation model of vertical datum will be modelled using calculated offsets along the coastal line. For suggesting the precise modelling method to vertical datum transformation, we analyzed results from various interpolation methods such as Spline and LSC method. As the results from analysis, the LSC method combined with 4-parameters trend model is more suitable for modelling the offsets between vertical datums. The final transformation model of vertical datum using the combination of LSC and 4-parameter model which provides the transformation accuracies of ${\pm}10.4cm{\sim}14.8cm$ level. And, the software for vertical datum transformation that was also developed using the final model in order to convert the height information included in various spatial data effectively. Therefore, the transformation model between vertical datums of land and sea part, which is developed in this study, is expected to minimize the confusion caused by mismatch of height information in the use of spatial data, and it also can be minimize economic and time losses in various application fields such as coastal development project, coastal disaster prevention, etc.

• THE JOURNAL OF KOREAN ORIENTAL ONCOLOGY
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• v.4 no.1
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• pp.71-87
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• 1998

Even though appropriate immune response is necessary for the survival of the individual, excessive or insufficient immune Response might cause autoimmune or allergic disease. So the immune response must be controlled to the degree that is beneficial for the well being of the individual. This study was undertaken to know the effects of Gunleetang Gagambang on the immune system of the mouse. Gunleetang Gagambang has been used for cure of tumor as a traditional medicine without any experimental evidence to support the rational basis for its clinical use. This study was carried out to evaluate the possible therapeutic or antitumoral effects of Gunleetang Gagambang extract against tumor, and to carry out some mechanisms responsible for its effect. Some kinds of tumor were induced by the typical application of 3-methylcholanthrene(MCA) or by the implantation(s.c) of malignant tumor cells such as leukemia cells(3LL cells) or sarcoma cells(S180 cells). Treatment of the Gunleetang Gagambang on water-extract(dailly 1mg/mouse, i. p.) was continued for 7 days prior to tumor induction and after that the treatment was lasted for 20 days. Against squamous cell carcinoma induced by MCA, Gunleetang Gagambang decreased not only the frequency of tumor production but also the number and the weight of tumors per tumor bearing mice(TBM). Gunleetang Gagambang on also significantly suppressed the development of 3LL cell and S180 cell-implanted tumors in occurrence-frequency and their size. and some developed tumors were regressed by the continuous treatment of Gunleetang Gagambang extract into TBM. In vitro, treatment of Gunleetang Gagambang extract had no effect on the growth of some kinds of cell line such as FsaII, A431 strain but significantly inhibited the proliferation of 3LL, S180 cells and augmented the DNA synthesis of mitogen-activated lymphocytes. Gunleetang Gagambang also stimulated the migrative ability of leukocyte, the MIF and IL-2 production of T lymphocytes, but not IL 6 production of B cells. Gunleetang Gagambang administration to mice enhanced NK cells activities. These results demonstrated that Gunleetang Gagambang extract exhibited a significant prophylactic benefits against tumors and its antitumor activity was manifested depending on the type of tumor cells. And these results also suggested that effect of Gunleetang Gagambang might be chiefly due to nonspecitie enhancement of NK cell activities and cell-mediated immune responses.