• Journal of Environmental Science International
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• v.11 no.2
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• pp.121-130
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• 2002

Compare to investigate the taxonomic utility of infragenera system and interspecific of Asian Geranium, we examined the fruit and seed morphology from 35 taxa. The fruit shape and seed's dispersal way did so that may divide subgenus or some part section to do could reason evolutionary trends. That is, at seed dispersal, the awns of section Geranium of subgenus Geranium, ramains attached at the top of rostrum, though fairy easily broken off, because seed increases awn's elasticity by diffusive way, it is long characteristic seed\`s dispersal distance, and subgenus Robertium observed that seed dispersal distance is short because it is no awn's elasticity by way that seed dispersal is gone as awn drops with rostrum. Also, section Geranium of subgenus Geranium and subgenus Robertium act role that awn keeps temporarily breed swerving with mericarp instead of bristle because section Tuberosa of subgenus Geranium is not bristled function that keep temporarily breed because mericarp bristled on base at seed dispersal do while. Therefore, is thought that is talon that when consider formation's development and function regarding this seed dispersal function, subgenus Geraniuum evolves more than subgenus Robertium. Seed morphology can divide by 2 subgenera(Geranium, Robertium) according to pattern of seed coat. and Seed's morphology characteristic(whole shape, color, form of seed apical and base, micropyle area, hilum area, chalaza, and position of seed hilum area), can distinguish some species, but is thought that is not reasonable as for discernment characteristic form and nature by repetition of characteristic form and nature. Especially, infrasection do in fruit and seed's morphology that is handling in this research that know to argue classification system and relationship by repetition of characteristic form and nature difficult. To all reliable truth, cytological, ecological, embryology, and molecular genetics research about talon is considered that should be achieved section Geranium that do not handle yet.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.55 no.1
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• pp.30-36
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• 2022

We collected a total of four butterfly ray specimens (Gymnura japonica, 213.4-695.0 mm in total length) in Korea from 2016 to 2021 and investigated their morphological and molecular characteristics in order to clarify their taxonomic status. These features are summarized as follows. Disc lozenge-shaped, 1.8-2.0 times broader than long. Tail very short, post-cloaca length 23.9-28.2% in disc width. Snout short, no rostral cartilage. Clasper short, no hook. Dorsal surface uniform yellow or brownish grey, with or without rounded light yellow spots. An analysis of 434 base-pair sequences of mitochondrial DNA cytochrome c oxidase subunit I showed that all four specimens corresponded to G. japonica from Japan (Kimura-2-parameter distance = 0-0.2%), suggesting that the color patterns found may be due to intraspecific color variation. G. japonica resembles Gymnura poecilura but differs in that it has a shorter tail length to disc width (23.9-28.2% in G. japonica vs. 40.1-48.3% in G. poecilura). This study revealed that G. japonica occurred in areas affected by the Tsushima Warm Current, tentatively suggesting that G. japonica may be an indicator species for monitoring marine ecosystem changes due to climate change.

• Fisheries and Aquatic Sciences
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• v.18 no.4
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• pp.405-410
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• 2015

The Korean lumpsucker, "Do-chi", reported previously as Eumicrotremus orbis, was reinvestigated on the basis of specimens collected from Korea, Japan, and the USA. Morphological and genetic analyses showed that "Do-chi" corresponds to Eumicrotremus taranetzi and clearly differs from E. orbis. Eumicrotremus taranetzi is readily distinguishable from E. orbis by its large, high spiny tubercles with weak, small or no prickles (small, low spiny tubercles with distinct prickles in E. orbis) and 3-4 pairs of spiny tubercles in the dorsal rows (five pairs in E. orbis). We compared partial sequences (466 bp) of the mitochondrial cytochrome c oxidase subunit I genes of "Do-chi" and other Eumicrotremus species. "Do-chi" and E. taranetzi were clustered by the smallest Kimura two-parameter genetic distance (d = 0.000-0.002) and were clearly separated from E. orbis (d = 0.035-0.037). Therefore, our results suggest that the scientific name of the Korean lumpsucker, "Do-chi" should be changed to E. taranetzi.

• Animal Systematics, Evolution and Diversity
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• v.16 no.2
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• pp.169-176
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• 2000

As one of researches to clarify the taxonomic status of roe deer from Jeju island (C. pygargus tianschanicus), we analyzed partial sequences of mtDNA cytochrome b gene from six roe deers at Jeju island in Korea. Maximum nucleotide Tamura & Nei's distance among three haplotypes was 0.005, and this distance was comparable to the diversity within other roe deer subspecies: it is suggested that roe deers from the mainland dispersed rarely to Jejudo Island, although further analyses are ne-cessary to decide whether or not it was occurred by human introduction. Furthermore, nucleotide distance between cytochrome b sequences of roe deers from Jeju (C. pygargus tianschanicus) and the sequence of roe deer from west Siberia(C. p. pygargus), obtained from GenBank, was average 0.013, and it is suggested that C. p. tianschanicus diverged from C. p. pygargus of west Siberia 0.65 Myr ago.

• Animal Systematics, Evolution and Diversity
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• v.28 no.4
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• pp.269-278
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• 2012

Gobiids are hyperdiverse compared with other teleost groups, with about 2,000 species occurring in marine, freshwater, and blackish habitats, and they show a remarkable variety of morphologies and ecology. Testing the effectiveness of DNA barcodes on species that have emerged as a result of radiation remains a major challenge in evolutionary biology. Here, we used the cytochrome c oxidase subunit 1 (COI) sequences from 144 species of gobies and related species to evaluate the performance of distance-based DNA barcoding and to conduct a phylogenetic analysis. The average intra-genus genetic distance was considerably higher than that obtained in previous studies. Additionally, the interspecific divergence at higher taxonomic levels was not significantly different from that at the intragenus level, suggesting that congeneric gobies possess substantial interspecific sequence divergence in their COI gene. However, levels of intragenus divergence varied greatly among genera, and we do not provide sufficient evidence for using COI for cryptic species delimitation. Significantly more nucleotide changes were observed at the third codon position than that at the first and the second codons, revealing that extensive variation in COI reflects synonymous changes and little protein level variation. Despite clear signatures in several genera, the COI sequences did resolve genealogical relationships in the phylogenetic analysis well. Our results support the validity of COI barcoding for gobiid species identification, but the utilization of more gene regions will assist to offer a more robust gobiid species phylogeny.

• Asian-Australasian Journal of Animal Sciences
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• v.6 no.1
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• pp.27-35
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• 1993

In an attempt to reveal the interrelationship between fowls of jungle and native origin, their gene constitutions were compared using gene frequencies at the 16 loci controlling blood protein variations. Of the 16 loci analysed by electrophoresis, polymorphism was detected at following seven loci: Es-1, Amy-1, Akp-akp, Akp-2, Alb, Tf and 6-PGD. The other nine loci: Amy-3, Es-D, PGM, PHI, MDH, To, LDH, Hb-1 and Hb-2, were noted to be monomorphic. Genetic distance between pairs of native fowl and jungle fowls was estimated by a numerical taxonomic method. The Indonesian native fowl was genetically close to the Indonesian red jungle fowl, and the grey jungle fowl was genetically similar to the Ceylonese jungle fowl. It was also suggested that the green jungle fowl was genetically remote from the other jungle fowls and from the Indonesian native flow. The proportion of polymorphic loci (Ppoly), the expected average heterozygosity per individual $\bar{H}$, and the effective number of alleles per locus (Ne) were calculated to evaluate the genetic variabilities in the native and jungle fowls. The Indonesian native fowl exhibited slightly higher the proportion of polymorphic loci than the jungle fowls.

• International Journal of Industrial Entomology and Biomaterials
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• v.10 no.2
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• pp.79-86
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• 2005

Molecular markers have increasingly been used in plant genetic analysis, due to their obvious advantages over conventional phenotypic markers, as they are highly polymorphic, more in number, stable across different developmental stages, neutral to selection and least influenced by environmental factors. Among the PCR based marker techniques, ISSR is one of the simplest and widely used techniques, which involves amplification of DNA segment present at an amplifiable distance in between two identical microsatellite repeat regions oriented in opposite direction. Though ISSR markers are dominant like RAPD, they are more stable and reproducible. Because of these properties ISSR markers have recently been found using extensively for finger printing, pohylogenetic analysis, population structure analysis, varietal/line identification, genetic mapping, marker-assisted selection, etc. In mulberry (Morus spp.), ISSR markers were used for analyzing phylogenetic relationship among cultivated varieties, between tropical and temperate mulberry, for solving the vexed problem of identifying taxonomic positions of genotypes, for identifying markers associated with leaf yield attributing characters. As ISSR markers are one of the cheapest and easiest marker systems with high efficiency in generating polymorphism among closely related varieties, they would play a major role in mulberry genome analysis in the future.

• International Journal of Industrial Entomology and Biomaterials
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• v.31 no.2
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• pp.40-47
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• 2015

The genus Acteniceromorphus is taxonomically reviewed for the first time in Korea. From the previously recorded three species, two species, A. selectus (Candèze, 1894) and A. fulvipennis (Lewis, 1894) are confirmed as misidientification of species which are endemic to Japan. Another species, A. chlamydatus (Lewis, 1894) is unavailable any Korean specimens. Additionally, we found A. ferrugineipennis (Candèze, 1879) as new to Korea. We also provide a comparison of DNA barcoding for two species previously misidentified and the newly recorded species, except A. chlamydatus. DNA barcoding result shows that each species is clearly delimited at species-level from each other by large interspecific genetic distance over 7.2%.

• Korean Journal of Ichthyology
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• v.21 no.sup1
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• pp.75-75
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• 2009

The previous morphology-based taxonomic frameworks within the family Sygnathidae had emphasized the significance of the male brood pouch and reproductive biology in defining the group. However, several different hypotheses had been proposed by different investigators. This study have beencarried out to determine the phylogenetic relationships among 19 species belonging to the order Syngnathiformes with three Gasterosteiformes species as outgroup taxa by using the mitochondrial cytochrome b and Rag2 nuclear DNA sequences. Phylogenetic analyses based on neighbor-joining distance, maximum parsimony, minimum evolution and maximum likelihood method strongly supported that the family Syngnathidae, the suborder Syngnathoidei and the order Syngnathiformes were all monophyletic group. Much of previous morphological analyses were supported by our molecular data, but some deep relationships were not clearly resolved with regard to members of the suborder Aulostomoidei.

• Journal of the Korean Society of Tobacco Science
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• v.5 no.2
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• pp.25-32
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• 1983

To obtain basic information on the breeding of burley tobacco, classification of 41 varieties was carried out by using the cluster analysis of correlation coefficients and taxonomic distance based on twenty-one agromonic characters. Eight characters, such as days to flowering, length of flower axis, internode length, leaf length, yield, leaf angle to stem, vein angle to midrib and plant height, were useful in monothetic classification. Forty-one varieties were classified into four groups (I, II, III and IV) with weighted variable group method (WVGM ) and weighted jai. group method(WPGM), whereas the results classification of 33 varieties among them by WVGM were coincident with the results by WPGM. As for the characteristics of each group, group I related to late maturity, tall height and high yield, group II related to intermediate maturity, tall height and low yield, group 19 related to early maturity, intermediate height and low yield, and group W related to early maturity, short height and intermediate yield.