• BMB Reports
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• v.46 no.3
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• pp.157-162
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• 2013

Human ${\alpha}$-galactosidase A (GLA) has been used in enzyme replacement therapy for patients with Fabry disease. We expressed recombinant GLA from Chinese hamster ovary cells with very high productivity. When compared to an approved GLA (agalsidase beta), its size and charge were found to be smaller and more neutral. These differences resulted from the lack of terminal sialic acids playing essential roles in the serum half-life and proper tissue targeting. Because a simple sialylation reaction was not enough to increase the sialic acid content, a combined reaction using galactosyltransferase, sialyltransferase, and their sugar substrates at the same time was developed and optimized to reduce the incubation time. The product generated by this reaction had nearly the same size, isoelectric points, and sialic acid content as agalsidase beta. Furthermore, it had better in vivo efficacy to degrade the accumulated globotriaosylceramide in target organs of Fabry mice compared to an unmodified version.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.5
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• pp.490-496
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• 2002

In order to detect the DNA heteropolymorphism of chum salmon, selected essential genes were examined in different regional chum salmons, i.e., Korean, Japanese and American by denaturing gradient gel electrophoresis (DGGE) and real time PCR methods. From the promoter regions and introns of growth hormone, mtDNA NDI region, D-loop region, IGF-I, histone H3 and MCH2 several representative primer pairs were obtained and employed for the DGGE with the PCR products from the genomic DNAs of the different regional chum salmons. mtDNA NDI, D-loop region and IGE-I genes showed marked heteropolymorphism between Korean and American chum salmons. Intron C of growth hormone also showed a heteropolymorphism between Korean and Japanese chum salmons. Whereas heteropolnnorphism of histone liH and MCH2 genes was detected among in Korean, Japanese and Asnerican chum salmons in the examined region. The real time PCR disclosed the characteristic incremental production of target DNAs dependent on the heteropolymorphic conditions of genomic DNAa of chum salmons, thus the different regional chum salmons could be grouped by the variable incremental curies. Although the DGGE and real time PCR did not produce the identical results in this study, we suggest that the DGGE and real time PCR could be used for the primary screening of the DNA heteropolymorphism of different animal genome.

• International Journal of Advanced Culture Technology
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• v.9 no.4
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• pp.18-28
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• 2021

This study surveyed how retirees' evaluation of starting food service business affects the effectiveness their new business and quality of life, based on personal factors such as entrepreneurship and business-starting capability and environmental factors by using questionnaires. Bootstrapping was carried out in order to find out factors affecting rapidly changing new business environments and retirees' initial intent to start a business so as to verify basic hypothesis about relation between retirees' evaluation of starting food service business (social, economic, and psychological effects) and the effectiveness of their new business and quality of life and confirm whether the effectiveness of the new business acts as a medium between the evaluation of starting food service business and quality of life. In addition, PLS-MGA was performed in order to verify whether the correlations among test factors can be varied according to the kind of job the target retirees had. Having examined the basic thesis, it was found that social and economic factors had significant positive effect on the effectiveness of the new business, and social and psychological factors had significant positive effect on the quality of life. Having analyzed whether the effectiveness of new business acted as a media between evaluation of starting food service business and quality of life, there was no significant effect as a medium. Having studied whether the kind of job of the retirees controlled or affected the relations among evaluation of starting food service business, effectiveness of new business and quality of life, the results were as follows: in the office job retiree group, the greater economic factor led to increase of effectiveness of new business, while social and psychological factors influenced the quality of life; In the physical labor group, the higher social factor resulted in higher effectiveness of new business, which showed significant positive effect on the quality of life. Having researched about which element is considered to be most important in starting food service business, the most important element was found to be dish/menu, followed by staff management, accounting management, business management, and service education. Having analyzed relation between accomplishment and important consideration for starting food service business, "managers with entrepreneurship," "appropriate distribution of time to prepare for starting business," and "operation of practical field education programs" showed higher importance compared to the degree of satisfaction, so it is needed to more concentrate on the above matters. This study intends to raise retirees' awareness of starting business and help them live better life based on the analysis results, and further suggest detailed mechanism and specific operations of factors affecting retirees' decision making on starting business, such that they can use the information as basic materials to make better choices that can lead to successful business.

• The Journal of the Korean dental association
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• v.55 no.2
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• pp.139-155
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• 2017

The study of job satisfaction among female dentists is essential to improve the quality of their life. In this study, a job satisfaction survey of female dentists in Gwangju was performed and the factors affecting job satisfaction were examined. A survey of female dentists in Gwangju was conducted using a custom-designed and validated questionnaire which incorporated the Korean Dentist satisfaction Survey (KDSS) as well as questions regarding socio-demographics and professional characteristics. Satisfaction for each question was measured by a 5-point Likert scale. The final analysis was performed with 89 questionnaires, after excluding 3 non-response questionnaires. Analysis of the data for each group was carried out using a Mann-Whitney U test and Kruskal-Wallis H test. The reliability of the questionnaire was identified by the internal consistency of the survey using Cronbach coefficient. The data were statistically analyzed using SPSS (IBM SPSS Statistics 19, SPSS Inc, Chicago, Ill, USA). The level of statistical significance was set at p = 0.05. On analyzing with use of the above mentioned method, the following findings were observed: Income, leisure time, time for self-development, and overall job satisfaction were lower than the average, especially the percentage of dissatisfaction was much higher than that of satisfaction. Satisfaction was associated with age, duration of employment, and the number of assistants. The limitations of this study are the lack of an adequate sample size, convenience sampling during extraction, the lack of questionnaire items, the limitations of quantitative research, and failure to perform measurement in the non-response group. However, female dentists can be expected to achieve a more satisfactory and enhanced working environment if the research based on the current study is followed up in an expanded target group.

• The Plant Pathology Journal
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• v.31 no.2
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• pp.123-131
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• 2015

Clavibacter michiganensis subsp. sepedonicus (Cms) multiplies very rapidly, passing through the vascular strands and into the stems and petioles of a diseased potato. Therefore, the rapid and specific detection of this pathogen is highly important for the effective control of the pathogen. Although several PCR assays have been developed for detection, they cannot afford specific detection of Cms. Therefore, in this study, a computational genome analysis was performed to compare the sequenced genomes of the C. michiganensis subspecies and to identify an appropriate gene for the development of a subspecies-specific PCR primer set (Cms89F/R). The specificity of the primer set based on the putative phage-related protein was evaluated using genomic DNA from seven isolates of Cms and 27 other reference strains. The Cms89F/R primer set was more specific and sensitive than the existing assays in detecting Cms in in vitro using Cms cells and its genomic DNA. This assay was also able to detect at least $1.47{\times}10^2copies/{\mu}l$ of cloned-amplified target DNA, 5 fg of DNA using genomic DNA or $10^{-6}$ dilution point of 0.12 at $OD_{600}$ units of cells per reaction using a calibrated cell suspension.

• Journal of Korean Elementary Science Education
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• v.25 no.4
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• pp.339-351
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• 2006

The purpose of this study was to analyze the processes of observation teaching and the types of observation in elementary life science class. For the purposes of this study, 40 teachers majoring in elementary science education participated in surveys on the processes of observation teaching. In addition, after videotaping the observation classes conducted by three teachers, each teaching in the 3rd to 5th grade, and their one hundred and eight students, we analyzed the processes of observation teaching. As a result, the processes of observation leaching in elementary science class were categorized into 12 stages (motivation, stating the objective, recognizing objects to observe, free and subjective observation, introducing ways to observe, observing by the simple way, introducing additional ways, observing by the manipulative way, making a note of observation, looking over the contents of observation, finding regularities, consolidation). In the types of observation, teachers and students performed mostly simple observing by the visual way. Teachers introduced time-independent/non-compared/whole observing; students concentrated on time-independent/non-compared/particular observing as targets. Thus, students carried out observing in the types of observation that teachers had presented. Consequently, the analysis about the processes of observation teaching can establish an effective teaching program for observing in scientific activities by reconstructing its observing target and suitable topic. The analysis about the types of observation can be applied to set up strategies for improving observation skills.

• Industry Promotion Research
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• v.7 no.4
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• pp.85-95
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• 2022

This study intends to analyze the meaning of symbolism in ancestral memorial rite arrangemen from the view that ancestral worship connecting traditional society with modernity are the transmission of ritual. It appears as a change the theory of Yin-Yang and Five Elements that became the basis and ideology about the Confucian view of life and death, an understanding of the universe structure, and a change in the four seasons. Ancestral memorial rite arrangements acknowledge the existence of ancestors. And it is understood as a ceremonial instrument which the living and the dead communicate spatially with time through the symbolic system. In addition, the four seasons, spaces of the skyground and underground were symbolized and embodied through the selection and arrangement of ancestral memorial rites. In the modern ancestral memorial rite arrangement, the factors that determine the location require Time-space analysis of the target. This is because the offering is understood not only as a functional role but also as a temporal and spatial symbolism to be expressed through the offering. In this study, it is meaningful to consider it from the perspective of inheritance of ancestral worship culture through discussions about the ideological background and symbolic system that appeared in ancestral memorial rite arrangement

• Journal of Life Science
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• v.31 no.9
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• pp.849-855
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• 2021

Recently, as nanotechnology has been introduced and used in various fields, the development of new drugs has been accelerating. Nanoparticles have maintained blood drug concentration for extended periods of time with a single administration of the drug. The drug can then be selectively released only at the pathological site, thereby reducing side effects to other non-pathological sites. In addition, nanoparticles can be modified for selective target sites delivery for other specific diseases, with polymers being widely used in the manufacture of these nanoparticles. Poly (D,L-lactic-co-glycolic acid ) (PLGA) is one of the most extensively developed biodegradable polymers. PLGA is widely used in drug delivery for a variety of applications. It has also been approved by the FDA as a drug delivery system and is widely applied in controlled release formulations, such as in gene therapy treatments. PLGA nanoparticles have been developed as delivery systems with high efficiency to specific cell types by using passive and active targeting methods. After the development of a drug delivery system using PLGA nanoparticles, the drug is selectively delivered to the target site, and the effective blood concentration for extended periods of time is optimized according to the disease. In this review paper, we focus on ways to improve cell-specific treatment outcomes by examining the development of astrocyte selective nanoparticles based on PLGA nanomaterials for gene therapy.

• Journal of Applied Biological Chemistry
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• v.62 no.3
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• pp.251-255
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• 2019

The effect of soaking temperature on the moisture uptake and the protein loss of soybeans during soaking process investigated. As the soaking temperature increased, the soaking rate significantly increased and Peleg model was suitable for describing the soaking characteristics of the soybean with high $R^2$ values (>0.991). The soaking time to achieve the target moisture content of soybean (130%) was estimated to be 12.6, 3.11 and 2.31 h at 25, 35 and $45^{\circ}C$, respectively. Peleg model well described the protein loss kinetics of soybean during soaking with high $R^2$ values (>0.941). The results showed that the protein loss of soybean at the target moisture content were 35.2, 93.1 and 103.0 mg/g at 25, 35 and $45^{\circ}C$, respectively. In this study, the optimum soaking condition for quality of soybean was 12.6 h of soaking time at $25^{\circ}C$.

• Journal of the Korean Institute of Landscape Architecture
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• v.43 no.6
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• pp.25-40
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• 2015

Korean tourist areas are designated/managed in accordance with the Tourism Promotion Act. Since Taejongdae was designated as a tourist area in 1969, a total of 230 tourist areas have been designated/built/operated. It has been 46 years since the first tourist area was designated. According to the Tourism Area Life Cycle by Butler, it is estimated that the flow of the life cycle will be reflected in ups and downs over time. Thus, this study aimed to provide basic data for suggesting the direction of changes and development of the future tourist areas after analyzing the life cycle stage of domestic tourist areas, by applying the Tourism Area Life Cycle by Butler. The research method was based on the tourist areas by year, the number of visitors, and data of the target to derive the change transition curve, obtained by dividing the life cycle stages of the tourist areas based on the visitor rate of change. In the analysis results, more than 1/3 of domestic tourist areas are reaching the stagnation/decline stage, and tourist areas such as hot springs and seaside/beach resources show a particularly high ratio of stagnation/decline. The tourist areas that already have reached the stagnation/decline stage will need to analyze the causes for the decline, seek for resolution measures, and introduce new innovative elements. Even though the results of this study are not sufficient to be used as an absolute standard to decide the life cycle stage of domestic tourist areas, it is considered to be adequate for phenomenologically understanding the life cycle stage of Korean tourist areas. Based on this study, the causes for the stagnation/decline of tourist areas can be revealed while it can be also used as basic research to establish revitalization measures for tourist areas by introducing new innovation.