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Analyses of Microbiological Contamination in Cultivation and Distrubution Stage of Tomato and Evaluation of Microbial Growth in Tomato Extract (토마토의 생산·유통단계에서 유해미생물 오염 및 추출물에서 미생물 증식)

  • Yun, Hyejeong;Park, Kyeonghun;Ryu, Kyoung-Yul;Kim, Byung Seok
    • Journal of Food Hygiene and Safety
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    • v.28 no.2
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    • pp.174-180
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    • 2013
  • This study investigated the microbiological contamination of tomato in cultivation and distribution stage. Growth of Escherichia coli O157:H7 and Listeria monocytogens examined in tomato extracts (0.1, 1.0, and 10.0%) and incubation temperatures (5, 15, 25, and $35^{\circ}C$). In cultivation stage of tomato, total aerobic bacteria were 7.77 log CFU/g in gloves of APC (Agricultural Products Processing Center) worker and Bacillus cereus were 0.33 log CFU/g at nutrient tank, respectively. And Staphylococcus aureus, Salmonella spp., were not detected. After APC stage, total aerobic bacteria were significantly higher compared with before-APC stage. Among of general, pesticide-free and organic produce in tomato were no significant difference in microbial contamination. Coliforms of tomato in small vinyl package were significantly higher when compared to tomato in whole boxes package. There was no significant difference in bacteria count between unwashed tomato and washed tomato using tap water for one minute. The growth of E. coli O157:H7 and L. monocytogens in tomato extracts were decreased significantly as the concentration increased, and the microbial population was reached the lowest point during storage in 10% tomato extracts concentration for 72h at $5^{\circ}C$. However, the population of E. coli O157:H7 and L. monocytogens were gradually increased at 7.33~8.51 and 7.73~8.60 log CFU/ml during storage at $15{\sim}35^{\circ}C$ for 72h, respectively.

Study on the Quality Characteristics of Pork By-product on the Different Pretreatment Process (돈육 부산물의 전처리 조건에 따른 품질특성 연구)

  • Kim, Tae-Kyung;Ku, Su-Kyung;Lee, Hye-Jin;Lee, Cheol-Won;Kim, Young-Boong;Jeon, Ki-Hong;Choi, Yun-Sang
    • Korean journal of food and cookery science
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    • v.32 no.6
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    • pp.716-723
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    • 2016
  • Purpose: This study was conducted to improve the quality characteristics of pork intestine through different pretreatment processes. Methods: We washed pork intestine by both physical (tap water, UV, and sonication) and chemical methods (alcohol, acetic acid, flour and NaCl) as pretreatment process. The physicochemical (pH, color, volatile basic nitrogen (VBN), and 2-thiobarbituric acid reactive substances (TBARS)) and microbial properties of pre-treated pork small intestine were evaluated. Results: The nature of the pretreatment method influenced the pH value of pork small intestine. The acetic acid treatment resulted in the lowest pH value. In physical method, the color value and the number of microorganism were significantly affected by sonication as compared to other treatments. TBARS value of pork small intestine after all the treatments was lower than the control. However, VBN exhibited no significant differences in its value irrespective of the nature of treatment. Appearance and control exhibited lowest value in response to sonication treatment. However, off-flavor and overall acceptability were higher in sonication treatment than other treatments. In chemical method involving NaCl and flour treatments, lightness and redness were lower than other treatments. Lowest VBN and TBARS values were noted in alcohol and acetic acid treatmentsand no growth of E. coli and coliform bacteria was observed. The other treatments resulted in lower values of VBN, TBARS, and microbial counts than the control. Appearance and color value after alcohol, acetic acid, and flour treatment were lower than the control and NaCl treatment. Off-flavor and overall acceptability of by-product after alcohol, flour, and NaCl treatments were higher than the control and acetic acid treatment. Conclusion: Overall, we present NaCl treatment and sonication treatment in the form of a combination pretreatment method as the optimal condition for processing pork intestine.

Effects of Chicken Feet Gelatin on Physicochemical and Sensory Properties of Restructured Chicken Jerky (닭발 젤라틴 첨가가 재구성 닭고기 육포의 이화학적 및 관능특성에 미치는 영향)

  • Kim, Hack-Youn;Lee, Jong-Wan;Kim, Ji-Hyuk;Kim, Gye-Woong
    • Korean Journal of Poultry Science
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    • v.42 no.4
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    • pp.327-333
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    • 2015
  • This study aimed to investigate the effect of chicken feet gelatin on physicochemical and sensory properties of restructured chicken jerky. Chicken feet swollen with hydrochloric solution (0.1 N HCl) were neutralized with flowing tap water, and gelatin was extracted with hot water at $75^{\circ}C$. The obtained chicken feet gelatin was dehydrated via freeze-drying. Restructured chicken jerky samples were prepared by adding the following amount of chicken feet gelatin 0%, 1%, 2%, 3%. The moisture and protein content of samples increased with an increased chicken feet gelatin. In addition, the drying yield of the samples increased with an increase in chicken feet gelatin. However, the shear force of samples significantly decreased with the increasing chicken feet gelatin content and the shear force of the control samples was the highest (P<0.05). No significant differences, except for color, were observed in the sensory analysis among the treatments. Therefore, usages of chicken feet gelatin can provide improved quality characteristics of restructured chicken jerky.

Evaluating Seismic Performance of Steel Welded Moment Connections Fabricated with SN Steel (SN 강재가 사용된 강구조 용접모멘트접합부의 내진성능 평가)

  • Oh, Sang-Hoon;Choi, Young-Jae;Yoon, Sung-Kee;Lee, Dong-Gue
    • Journal of Korean Society of Steel Construction
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    • v.22 no.3
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    • pp.271-280
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    • 2010
  • This study was programmed to fabricate a beam-to-column connection that is limited to a steel-welded moment connection with full-scale members, using SN steel. A cyclic seismic test was conducted of the nine specimens that were fabricated by choosing the test variable for the weld access hole geometry, connection design method, and RBS. From the test results, failure modes, the moment-drift behavior, and the strain distribution were provided. From the specimen material properties, the beam's nominal plastic flexural capacity and classified qualified connection as a special moment flame were calculated. By analyzing the skeleton part and the baushinger part, a range of strength-raising effects, and deformation ratios were provided, with which the seismic performance of the specimens were evaluated. The test results showed that the specimens eliminated their weld access holes that demonstrated higher seismic performance than the specimens' existing weld access holes, and that the WUF-W connection that was reinforced by the supplemental fillet weld around the shear tap that was fastened by five bolts demonstrated superior seismic performance.

Trend analysis of Domestic water Consumption Depending upon the characteristics of using tap water and economical parameters (수돗물 사용특성과 경제적 요인에 따른 가정용수 소비 경향의 분석)

  • Choi, Sun-Hee;Kim, Sang-Hyun
    • Proceedings of the Korea Water Resources Association Conference
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    • 2007.05a
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    • pp.121-125
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    • 2007
  • 물 수요예측을 실시함에 있어서 사회 경제의 장래동향과 도시의 특성 및 발전 동향을 반영하여 수행하여야 한다. 그러나 지금까지의 관행으로는 수도계획에서 사용할 수 있는 실사용량에 대한 조사와 통계가 이루어지지 않고 있다. 실제 현장에서 얻은 자료를 토대로 하여 수도계획 및 설계에 사용할 수 있는 신뢰성 있는 설계인자의 도출이 필요하다. 본 연구에서는 급수지역의 각 조사 가정의 물 사용량을 실측 조사하여 얻어진 자료를 토대로 하여 물 수요 구조의 변화를 파악할 수 있는 수단의 하나로 가정의 수돗물 사용특성과 경제적 요인에 따른 가정용수의 사용특성 경향을 파악하고자 하였다. 가정에서 사용된 용수량의 조건별 경향성을 분석하기 위하여 한국수자원공사에서 2002년부터 2006년까지 3년여에 걸쳐 표본 집단이 되는 전국 140개 가구에 유량계를 설치하여 유량 자료를 획득하였고, 설문조사를 실시하여 각 가정의 물 사용 특성인자들을 조사하였다. 이 자료를 대상으로 비교적 자료의 신뢰성을 높이기 위한 자료의 선별과정을 거쳤다. 이렇게 선별된 자료들을 경향성 분석에 쓰이는 Mann-Kendall test와 Spearman's Rho test를 이용하여 분석하였다. 그 결과를 바탕으로 가정 용수 소비패턴의 증가 감소에 영향을 끼치는 인자들을 추출하였다. 실측자료를 분석을 통하여 나타난 결과들을 바탕으로 용수 수요처의 조건별 특성을 분석하고, 이를 활용한 생활용수 예측모형을 개발함으로써 합리적인 수요예측에 의한 용수수요의 과다예측 우려를 해소하고, 경제적 수도시설계획을 수립하는 등 과학적 물 수요관리 정책 수립을 위한 의사결정도구가 제공될 수 있다.c}$C의 저온에서 저장한 감자는 $20^{\circ}$C에서 저장한 감자보다 발아의 지연과 함께 낮은 PGA증가율을 보였다.다 높았으며, 전반적인 선호도의 경우 G3(1.5%)를 가장 선호하는 것으로 나타났다. 백년초 분말 첨가 도토리묵의 경우 색상은 0.5% 첨가한 O1이 가장 높은 값을 나타냈으며, 외관은 1.5% 첨가한 O3가 가장 높은 값을 보였다. 향미와 신맛의 경우는 백년초 분말의 첨가량이 증가됨에 따라서 유의적으로 증가했다(p<0.001). 씹힘성, 탄력성, 견고성의 경우는 대조군이 가장 높았으며, 백년초 분말 첨가량이 증가함에 따라 유의적으로 낮아졌다(p<0.001), 떫은맛의 경우는 백년초 분말에 의한 신맛의 영향으로 1% 백년초 분말이 첨가된 O2 가장 높게 나타났다. 전반적인 선호도는 0.5% 백년초 분말이 첨가된 O1이 가장 높게 나타났다. 따라서 녹차와 백년초의 기능성을 살린 도토리묵을 실용화시키는 효과적인 배합비는 녹차 분말 1.5% 첨가와 백년초 분말 첨가 0.5%가 바람직한 것으로 보이며 백년초 분말은 1%까지도 가능한 것으로 나타났다. 그러므로 기능성 식품 소재로써 도토리묵에 녹차와 백년초 분말을 첨가하는 것은 충분히 활용할만한 가치가 있다고 사료된다.론적으로, 비육돈 사료 내 3.32%의 호맥 사일리지의 혼합급여는 혈액 내 코티졸 함량, 도체육의 명도와 황색도, 지방산 조성 및 영양소 소화율에 영향을 미치는 것으로 사료되나 이에 대한 보다 많은 연구가 필요할 것으로 판단된다.니티와 공원과의 관계로 공원 설계와 관리에 있어서 영국에서는 커뮤니티가 직접 고객(client)으로서 역

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Use of Carbon Nanotube Electrode and Squarewave Anodic Stripping Voltammetry for the Detection of Lead Heavy Metal (납 중금속 검출을 위한 탄소나노튜브 전극 및 네모파 양극 벗김 전압전류법 이용)

  • Choi, Changkun;Seok, Jonghyuk;Kwon, Yongchai
    • Applied Chemistry for Engineering
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    • v.23 no.5
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    • pp.505-509
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    • 2012
  • In this study, we investigate the use of new carbon nanotube paste electrode (CNPE) for promoting the detection of lead (Pb) heavy metal in the a drinkable water, which negatively affects human brain and nerve system. For the evaluations, CNPE is served as a working electrode, while sensitivity and limit of detection (LOD) of Pb are measured in DI and tap water based electrolytes using squarewave anodic stripping voltammetry (SWASV). As a result of that, in the 25~150 ppb range of $Pb^{2+}$ ions, its sensitivity and calculated LOD are $12.85\;{\mu}A/{\mu}M$ and 26 ppb in DI water based 0.1 M $H_{2}SO_{4}$ electrolyte while they are $10.36\;{\mu}A/{\mu}M$ and 38 ppb electrolytes respectively. In addition, experimentally measured LOD values of Pb are 4 ppb and 10 ppb in the two water electrolytes. The stripping of $Pb^{2+}$ ion is also controlled by surface reaction. Our experimental data are then compared with those of other already published references. With the comparison, it is proved that our electrode outperforms other electrodes in terms of the sensitivity and LOD of trace Pb metal.

Analysis of Growth Characteristics and Physiological Disorder of Korean Ginseng Affected by Application of Decomposing Plant Residues in Paddy-Converted Field (인삼 논재배 시 식물성 유기물 시용 수준에 따른 인삼 생육특성 및 생리장해 분석)

  • Jang, In Bae;Hyun, Dong Yun;Lee, Eung Ho;Park, Kee Choon;Yu, Jin;Park, Hong Woo;Lee, Sung Woo;Kim, Gi Hong
    • Korean Journal of Medicinal Crop Science
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    • v.22 no.2
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    • pp.140-146
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    • 2014
  • This study was carried out to investigate the difference of the content of soil chemical components and growth characteristics in six years old ginseng affected by application of decomposing plant residues in paddy-converted field. The results show that aerial parts of ginseng are no difference between press cake (PC) 200 kg/10a and control but subterranean parts of ginseng PC 200 kg/10a, especially quantity related root fresh weight and tap root diameter, are statically about 1.6 times heavier and about 1.2 times thicker than the ginseng control. Furthermore, the survival rate of PC 200 kg/10a is 67.1% rise significantly compare with the control 50.7%. But compared with the PC 200 kg/10a and the PC 400 kg/10a, ginseng root growth and survival rate of PC 400 kg/10a get worse and that increase physiological disorder occurrence rate than PC 200 kg/10a. Even though there are no significant differences between the ginseng of decomposing plant residues except press cake treatment and the ginseng of control in growth characteristics, it does tend to increase the survival rate and decrease the physiological disorder occurrence rate in most fertilizer treatment except for RSC 2 ton/10a, RSC 4 ton/10a and RH 4 kL/10a. Noted that EC is highly increased and exceeded 1.7 ds/m in RSC 2 ton/10a, RSC 4 ton/10a and RH 4kL/10a. It would be caused physiological disorder in many ways and affected ginseng growth characteristics, survival rate.

Preparation and properties of gelatin from conger eel skin (붕장어껍질로부터 젤라틴의 제조 및 그 특성)

  • Ihm, Chi-Won;Kim, Poong-Ho;Kim, Jin-Soo
    • Applied Biological Chemistry
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    • v.39 no.4
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    • pp.274-281
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    • 1996
  • To prepare edible skin gelatin of conger eel such as material fur quality improvement of surimi gel, the defatted skin was limed with 1% calcium hydroxide at $5^{\circ}C$ for 2 days, washed thoroughly with tap water, extracted with 8 volumes of distilled water to dehydrated skin for 2 hours at $50^{\circ}C$. The gelatin extract was centrifuged, filtered and then passed through anion(Amberlite 200C) and cation (Amberlite IR 900) resins. The purified gelatin solution was evaporated and dried by hot-air blast$(40^{\circ}C)$. The gelatin prepared by above condition had the highest quality as revealed by physical property values i.e. 240.5 g in gel strength, $28.0^{\circ}C$ in melting point and $28.0^{\circ}C$ in gelling point. Funtional property values were 56.8% in solubility, 1.8 ml/g in oil binding capacity, 55.0% in emulsifying capacity and 48.5% in emulsifying stability. jelly strength and senso교 evaluation of surimi gel from fish with red muscle were not improved by addition of emulsifying curd from conger eel skin gelatin as emulsifier. Therefore, the conger eel skin gelatin requires a suitable modification of functional group and improvement of processing operation to utilize as a material for quality Improvement of surimi gel.

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Tertiary Treatment of Municipal Wastewater Using Unsaturated Sandy Soil (불포화 사질토양을 이용한 도시하수의 3차 처리)

  • Kim, Seung-Hyun;Chung, Jong-Bae;Ha, Hyun-Soo;Prasher, Shiv O.
    • Korean Journal of Environmental Agriculture
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    • v.22 no.2
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    • pp.111-117
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    • 2003
  • Treatment of secondary effluent was investigated using sandy soil as a possible alternative to the tertiary treatment of municipal wastewater. Secondary effluent was applied with three different flow rates to the surface of pilot scaled lysimeters, which were filled with sandy soil. Some of the lysimeters were covered with osd, while others were kept bare in order to investigate the role of plantation on the treatment. The concentration changes in COD and nitrogen were measured along the unsaturated soil depth. The same set of experiment as with the secondary effluent was performed using tap water to investigate the dissolution of the contaminants from the soil. from the results it was found that when sandy soil was used for tertiary treatment of municipal wastewater COD removal efficiency reached about 70% regardless of the application rate. The soil depth needed to obtain such efficiency increased along with the application rate, which was about 60 cm at the application rate of 50 L/day. Results also showed that nitrification occurred rapidly. The process was completed in soil depth of first $10{\sim}20\;cm$. Nitrogen removal efficiency was as low as about 20% regardless of the application rate. Some supplementary means should be considered to improve the efficiency. Sod on the soil surface had no significant influence on the contaminant treatment but was helpful to keep the infiltration rate undiminished. Finally, the organic soil was found to release significant amount of contaminants when it was in contact with soil water.

Screening of saline tolerant plants and development of biological monitoring technique for saline stress. II. Responses of emergence and early growth of several crop species to saline stress. (내염성 식물의 탐색 및 생물학적 염해 모니터링 기술의 개발 II.염분 스트레스에 대한 작물의 출현과 초기 생장 반응)

  • Shim, Sang-In;Lee, Sang-Gak;Kang, Byeung-Hoa
    • Korean Journal of Environmental Agriculture
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    • v.17 no.2
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    • pp.122-126
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    • 1998
  • This experiment was conducted to verify the responses of major crop species to saline stress.To determine the saline tolerance of crop species, sevral crop species were cultivated under sand-culture system using Hoagland's nutrient solution whit 200mM NaCl. The crop species showing saline tolerance were cotton(Gossypium indicum), maize(Zea mays), barely(Hordeum vulgare), and wheat(Triticum aestivum) but perilla (Perilla frutescens) and leguminous crops, mung bean(Phaseolus radiatus), azuki bean(Phaseolus angularis) and soy bean(Glycin max) showed very por tolerance. One the typical symptom was the darkening of leaf color due to increase of chlorophyll concentration.Among of the plant families, Fabaceae was the most susceptible but crop species belonging to Poaceae were more proper for cultivating on reclaimed tidal land in the course of desalinazation. It was suggrsted that the crop species belonging to Fabaceae, a sensitive family to soil salinity, must be cultivated when the soil salinity decreased below 10ds/m. To know the critical salinity level for crop growth,salinity of saline soil collected from reclaimed tidal land was adjusted from 10.0ds/m tp41.7ds/m with tap water. It was suggested that the ECs of the soil in which the plant height of each crop spicies was reduced to 50% of control plant were 22.6 and 21.7 ds/m in rice, barley, corn, mung bean, and soy bean,respectively.

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