• 제목/요약/키워드: tannins

검색결과 233건 처리시간 0.023초

식물 유래 탄닌산의 접착능을 이용한 표면 개질 및 의료용 제형 기술 동향 (Surface Modification and Medical Formulation Technology Using Adhesion of Plant Tannic Acid)

  • 박은숙;신미경;이해신
    • 접착 및 계면
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    • 제20권2호
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    • pp.71-75
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    • 2019
  • 탄닌산은 식물계에서 가장 많이 발견되는 폴리페놀 중 하나로, 초기 탄닌산 연구는 항산화제 등과 같은 생리학적 기능에 집중되어 있었다. 그러나 최근에는 탄닌산이 단백질, DNA 등 거의 모든 생체고분자와 분자간결합을 하는 것이 밝혀짐에 따라 분자적 접착제로서 많은 관심을 받고 있다. 탄닌산의 다양한 특성들은 표면의 기능, 젖음성을 조절할 뿐 아니라 에너지 저장 및 발생 장치에 기여하고, 의학적 제재로의 다양한 가능성을 보이고 있다. 본 논문에서는 분자적 접착제로서의 탄닌산과 생체고분자와의 결합, 탄닌산을 통한 표면 개질, 의료용 제재로의 활용 등에 대해 다루고자 한다.

Gallic acid caused cultured mice TM4 Sertoli cells apoptosis and necrosis

  • Li, Wanhong;Yue, Xiangpeng;Li, Fadi
    • Asian-Australasian Journal of Animal Sciences
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    • 제32권5호
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    • pp.629-636
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    • 2019
  • Objective: The study was designed to determine the cytotoxic effect of gallic acid (GA), obtained by the hydrolysis of tannins, on mice TM4 Sertoli cells apoptosis. Methods: In the present study, non-tumorigenic mice TM4 Sertoli cells were treated with different concentrations of GA for 24 h. After treatment, cell viability was evaluated using WST-1, mitochondrial dysfunction, cells apoptosis and necrosis was detected using JC-1, Hoechst 33342 and propidium iodide staining. The expression levels of Cyclin B1, proliferating cell nuclear antigen (PCNA), Bcl-2-associated X protein (BAX), and Caspase-3 were also detected by quantitative real-time polymerase chain reaction and Western-blotting. Results: The results showed that 20 to $400{\mu}M$ GA inhibited viability of TM4 Sertoli cells in a dose-dependent manner. Treatment with $400{\mu}M$ GA significantly inhibited PCNA and Cyclin B1 expression, however up-regulated BAX and Caspase-3 expression, caused mitochondrial membrane depolarization, activated Caspase-3, and induced DNA damage, thus, markedly increased the numbers of dead cells. Conclusion: Our findings showed that GA could disrupt mitochondrial function and caused TM4 cells to undergo apoptosis and necrosis.

Phytochemical Characterization of Vitex negundo Leaves: a Potent Antiandrogenic and Antioxidant Agent

  • Sharath, Jayapal;Taj, Rafi Ahmed Shahin;Bhagya, Mahadevaiah
    • Natural Product Sciences
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    • 제28권3호
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    • pp.130-137
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    • 2022
  • This study was conducted to characterise phytochemicals and to explore the biological activities of Vitex negundo leaves. The washed, course powder of V. negundo leaves were extracted with different solvents of increasing polarity. All the extracts were characterized and biological activities were compared. The results revealed that the ethanolic and cold water extracts showed the presence of all phytochemicals studied except protein compared to other extracts. Further, the quantitative estimation of phytochemicals showed that the ethanolic extract had highest yield and maximum amount of total polyphenols, flavonoids, and alkaloids with the least amount of tannins compared to other extracts studied. Furthermore, the highest total polyphenol content corresponds with the potent biological activities. Indeed, in vitro antioxidant and antisteroidogenic activities were highest in the ethanolic extract than others. To conclude, the present study is the first to report the characterization and antiandrogenic property of V. negundo leaf extracts. The ethanolic extract of V. negundo leaves can be used as an antioxidant and antiandrogenic agent. Hence, it can be considered for the treatment of hyperandrogenic conditions like polycystic ovary syndrome, etc.

Inhibition Effects of Persicaria amphibia (L.) Delarbre on Oxidative DNA Damage via ATM/Chk2/p53 pathway

  • So-Yeon Han;Hye-Jeong Park;Jeong-Yong Park;Seo-Hyun Yun;Mi-Ji Noh;Soo-Yeon Kim;Tae-Won Jang;Jae-Ho Park
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2021년도 춘계학술대회
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    • pp.52-52
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    • 2021
  • Persicaria amphibia as an England native plant, is a rhizomatous perennial, one of the rather amphibious plants. Its aquatic form contains water-soluble sugars, starch, and protein. P. amphibia have up to 18% tannins in stems and rhizomes. Previous studies have confirmed the anti-inflammatory activity of live bacteria roots, but no studies on bioactivity are known. DNA damage responses (DDRs) pathways are considered a crucial factor affecting the alleviation of cellular damage. The ataxia-telangiectasia mutated and Rad3 related (ATM) and checkpoint kinase 2 (Chk2) pathways are the main pathways of DNA damage response. Also, p53 is a key integrator of cellular response to oxidative DNA damage, contributing repair, or leading transcription including apoptosis. In the present study, we conducted an investigation into the inhibitory effects of P. amphibia on oxidative DNA damage for confirming potential to complementary medicine and therapies. In conclusion, P. amphibia can provide protective effects against double-stranded DNA break (DSB) caused by oxidative DNA damage.

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지유(地楡)의 피부 노화(老化)에 대한 연구(硏究) (Study on Anti-Skin Aging Effect of Sanguisorba officinalis L.)

  • 김경신;탁동률;김병수;강정수
    • 혜화의학회지
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    • 제21권2호
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    • pp.63-72
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    • 2013
  • To develop a new anti-skin aging cosmetics or functional foods by using antioxidative activity and collagenase inhibitor, a potent collagenase or elastase inhibitor was screened from various extracts of medicinal plants and its optimal extraction condition was investigated. And antioxidative activity, antimicrobial activity and inhibitory of effect against collagenase activity were investigated. In the these results, we selected the Sanguisorba (Sanguisorba officinalis L.) that presents a potential biological activities. Sanguisorba which is very rich in triterpenoid saponin and tannins was recently reported its anti-oxidant activities and phytoestogenic activities in vivo test and many clinical studies. The experiments were carried out in vitro to determine anti-oxidant activities of Sanguisorba extracts on DPPH radical scavenging activity assay, Superoxidase scavenging activity assay, Elastase and collagenase activity assay. It show that the Sanguisorba extracts have the most significant anti-oxidant on free radical scavenging activity assay, and also inhibited significantly activities of elastase, collagenase. Further, Sanguisorba extracts are activated Type I collagen protein expression in CCD-986sk cells. These result suggest that the Sanguisorba extracts on DPPH radical scavenging activity assay, Superoxidase scavenging activity assay, elastase and collagenase activity assay, Type I collagen protein expression in CCD-986sk cells effected could be developed cosmetic ingredients for anti-aging.

Evaluation of Fermentation Characteristics and Nutritive Value of Green Tea Waste Ensiled with Byproducts Mixture for Ruminants

  • Kondo, Makoto;Kita, Kazumi;Yokota, Hiro-omi
    • Asian-Australasian Journal of Animal Sciences
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    • 제19권4호
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    • pp.533-540
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    • 2006
  • In this study, the possibility of green tea waste (GTW) as a new ingredient of byproducts-mixed silage was investigated. Characteristics of GTW were low in dry matter (DM) content (20%), and high in crude protein (30 to 36%) and tannins (8.5%). The GTW was added to mixed silages composed of tofu cake, rice straw and rice bran that are locally available in Japan. In experiment 1, the effect of GTW addition to silage made from various patterns of byproducts mixture based on tofu cake was studied. In experiment 2, the effect of GTW addition and storage temperature on fermentation characteristics, nutrient contents and in vitro ruminal gas production of byproducts-mixed silages were examined. In experiment 1, GTW addition on tofu cake accelerated acetic, propionic and butyric acid accumulation in the silage. When rice straw was mixed with tofu cake, DM content was increased from 47 to 56%, lactic acid was the main acid and the pH was decreased below 4.2. In this case, GTW addition to those mixtures did not affect acid concentrations of the silage. In experiment 2, GTW addition to the byproducts mixture increased lactic acid concentration, decreased the pH and DM loss of the silages. In GTW treatments, tannin concentration was lower in the silage stored at $30^{\circ}C$ than $15^{\circ}C$. Addition of GTW into the silage also increased in vitro ruminal gas production. It was concluded that addition of GTW into byproducts-mixed silage enhanced lactic acid fermentation when there were insufficient materials for lactic acid production. Utilization of GTW as an ingredient in mixed silages would be effective in enhancing fermentation characteristics, lowering tannin content and in vitro ruminal gas production.

복분자 열매(Rubus coreanus Miquel)의 항산화 활성 및 생리활성물질 (Antioxidant Activity and Effective Compounds of Black Raspberry (Rubus coreanus Miquel) Extracted by Different Solvents)

  • 진동혁;성종환;이영근;김동섭;정헌식;김한수
    • 한국응용과학기술학회지
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    • 제33권3호
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    • pp.474-482
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    • 2016
  • The black raspberry (Rubus coreanus Miquel) contains anthocyanin, tannins, gallotannin, gallic acid, ferulic acid and phenolics. It brightens the eyes and protects the liver and kidneys. It was effective for anti-aging. Thus, the purpose of this study was to inform the excellence of black raspberry and to screen antioxidant activity to ensure the possibility as a functional material. In this study, bioactive compounds in black raspberry were determined. Additionally, black raspberry was extracted by CM (chloroform:methanol, 2:1, v/v), 70% methanol and 70% ethanol, and were investigated and compared in vitro methods. Total phenol and flavonoid contents were measured to compare each different solvents. In addition, DPPH radical scavenging activity and ABTS radical scavenging activity, ferric reducing antioxidant power, reducing power were determined to measure the antioxidant activity. The results were the highest in 70% ethanol extracts, and the higher the concentration showed a significantly higher antioxidant capacity. When observed the relationship between the study, antioxidant activity of black raspberry was supposed to affect by the anthocyanin, phenol and flavonoid contents.

Anti-ulcer and antioxidant activity of leaves of Madhuca indica in rats

  • Chidrewar, G.U.;Tanavade, J.H.;Deshpande, S.H.;Vartak, P.S.;Shah, J.B.;Patel, N.P.;Patadiya, C.R.;Bafna, P.A.
    • Advances in Traditional Medicine
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    • 제10권1호
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    • pp.13-20
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    • 2010
  • The leaves of Madhuca (M.) indica J.f.Gmel. (Sapotaceae) have been used traditionally in folk medicine due to its astringent properties and are effective in treatment of eczema and snake bites. Methanolic extract of M. indica is rich in tannins and has been proven experimentally to possess antibacterial activity. The present study was undertaken to evaluate the anti-ulcer and antioxidant activity of M. indica in rats. The methanolic extract of leaves of M. indica was tested at various doses (75, 150 and 300 mg/kg, p.o.) for its effect on gastric secretion and gastric ulcers in pylorus-ligation and on ethanol- induced gastric mucosal injury in rats. The significant reduction in ulcer index in both the models along with an increase in the pH of the gastric fluid and mucin content of stomach, and the acid secretory parameters such as total acidity and volume of gastric fluid were also significantly reduced along with reduction in the pepsin activity in pylorusligated rats proved the anti-ulcer activity of M. indica. The increase in the levels of superoxide dismutase, catalase and reduced glutathione and decrease in lipid peroxidation in both the models proved the antioxidant activity of M. indica. Thus it can be concluded that M. indica possesses anti-ulcer activity, which can be attributed to its antioxidant mechanism of action.

전해산성수에 의한 저장기체살균이 포도의 품질특성과 부패균 생육에 미치는 영향 (Effect of Atmosphere Sterilization Using Acidic Electrolyzed Water on Storage Quality and Microbial Growth in Grapes)

  • 김성환;정헌식;이주백;강준수;정신교;최종욱
    • 한국식품영양과학회지
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    • 제32권4호
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    • pp.549-554
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    • 2003
  • 산성 전해수를 이용하여 포도(Vitis labrusca L.)의 저장 안정성을 향상 시킬수 있는 방법을 개발하기 위하여, Campbell Early와 Sheridan 포도를 저장고 내부기체를 산성 전해수에 통과시켜 살균시키는 $0^{\circ}C$ 조건에서 저장하면서 환경기체의 살균처리가 포도의 품질특성과 미생물 생육에 미치는 영향을 조사하였다. 저장환경기체의 살균처리는 포도 품종에 따른 차이가 없이 객관적 품질특성인 가용성 고형물, 적정산도, 가용성 탄닌, 안토시아닌, 에탄올 및 아세트 알데하이드 등의 함량 변화와 관능적 품질특성인 color, aroma, texture, juiciness 및 sweetness등에 거의 영향을 미치지 않는 것으로 나타났다. 반면에 저장기체의 살균처리는 포도 품종에 약간 의존적이긴 하지만 곰팡이를 포함하는 미생물의 생육을 억제하는 효과를 보였다. 이러한 결과로 볼 때 산성 전해수에 의한 저장환경기체의 살균처리는 포도의 품질에는 해가 없으면서 부패를 억제시켜 저장 안정성을 향상시킬 수 있는 방법으로 판단된다.

Chemical Composition of Some Tropical Foliage Species and Their Intake and Digestibility by Goats

  • Kongmanila, Daovy;Ledin, Inger
    • Asian-Australasian Journal of Animal Sciences
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    • 제22권6호
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    • pp.803-811
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    • 2009
  • The chemical composition and water extractable dry matter (DM) of foliages from Erythrina (Erythrina variegata), Fig (Ficus racemosa), Jackfruit (Artocarpus heterophyllus), Jujube (Ziziphus jujuba Mill), Kapok (Ceiba pentandra) and Mango (Mangifera indica) and the feed intake, digestibility and N retention when feeding these foliages were studied in two experiments. In Experiment 1, 12 male goats, 3.5 months old and weighing 14.6 kg, were randomly allocated to a diet consisting of one of the foliages in a change-over design with three periods. The foliages were offered ad libitum at the level of 130% of the average daily feed intake. The Erythrina foliage had a low content of DM and condensed tannins (CT) and a high concentration of crude protein (CP) in leaves plus petioles (193 g/kg DM) and stem, while the Mango foliage had a low CP (69 g/kg DM) and high DM content. The other foliages were intermediate. High content of CT was found in the leaves plus petioles of Jackfruit foliage and in the stem of Fig and Mango foliage. There was a difference in feed intake, nutrient intake, apparent digestibility and N retention between the foliages, with Erythrina, Jackfruit and Kapok foliage being significantly higher in these parameters than Fig, Jujube and Mango foliage. The water extractable DM could be used to estimate N retention, but not DM digestibility in this study. In Experiment 2, 4 male goats weighing 13.4 kg and 6 months old were allocated to a 4${\times}$4 Latin square design. The treatments were: water spinach ad libitum and Fig, Jujube or Mango foliage ad libitum +0.5% of BW as water spinach DM. Feed intake, apparent digestibility and N retention were not significantly different among the foliage diets, but higher than for water spinach alone (p<0.05). Supplementation with water spinach to a diet consisting of low quality foliages such as Fig, Jujube and Mango, increased DM and CP intake, apparent digestibility and N retention, compared to feeding these foliages as sole feeds.