• Journal of Microbiology and Biotechnology
• /
• 제14권1호
• /
• pp.162-166
• /
• 2004

Phage P22 tailspike is a thermostable homotrimeric protein, and temperature-sensitive folding (tsf) and global suppressor mutations affect its folding yields at elevated temperatures. We earlier suggested that the folding of the tailspike protein in Escherichia coli requires an unidentified molecular chaperone. Accordingly, in the present study, the interactions of purified DnaK, DnaJ, and GrpE heat-shock proteins with the tailspike protein were investigated during the translation and folding of the protein. The cotranslational addition of DnaJ to the tailspike protein resulted in the arrest of folding, when Dnak and GrpE were missing. However, the presence of DnaK, DnaJ, and GrpE had no effect on the folding yield of the tails pike protein, thus, providing evidence for the binding of the nascent tailspike protein with DnaJ protein, a member of DnaK chaperoning cycle.

• KSBB Journal
• /
• 제11권6호
• /
• pp.704-711
• /
• 1996

경제성분석 기능을 가진 생물공정 flowsheeting 소프트웨어인 BioPro Designer를 이용하여 외부단 백질 생산 시스템의 최적 공정설계가 수행되었다. BioPro Designer를 이용하여 Salmonella phage P P22의 tailspike protein의 생산시스템에 적용되었 는데 최적공정을 선택하는 과정에서 투입되는 시간 과 노력에 획기척인 개선이 있었다. 숙주의 내부에 서 생산되는 외부 단백질의 folding 특성이 가장좋 은 생산시스템을 선택하는데 영향을 미 친다는 것을 보여주기위하여 wild type의 tailspike와 두개의 다른 변종 tailspike들, 즉 tsf G244→R과 Su A334 -V을 비교 검토되었다. 회수된 tailspike 단백질의 단위질량 대비 자본투자비를 비교 척도로 하여 최척 의 tailspike 생산 시스템이 선택되었다.

• Journal of Microbiology and Biotechnology
• /
• 제19권9호
• /
• pp.898-903
• /
• 2009

Pullorum disease affecting poultry is caused by Salmonella enterica serovar Pullorum and results in severe economic loss every year, especially in countries with a developing poultry industry. The pathogenesis of S. Pullorum is not yet well defined, as the specific virulence factors still need to be identified. Thus, to isolate specific DNA fragments belonging to S. Pullorum, this study used suppression subtractive hybridization. As such, the genome of the S. Pullorum C79-13 strain was subtracted from the genome of Salmonella enterica serovar Gallinarum 9 and Salmonella enterica serovar Enteritidis CMCC(B) 50041, respectively, resulting in the identification of 20 subtracted fragments. A sequence homology analysis then revealed three types of fragment: phage sequences, plasmid sequences, and sequences with an unknown function. As a result, several important virulence-related genes encoding the IpaJ protein, colicin Y, tailspike protein, excisionase, and Rhs protein were identified that may play a role in the pathogenesis of S. Pullorum.