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Sequence variation of necdin gene in Bovidae

  • Peters, Sunday O.;Donato, Marcos De;Hussain, Tanveer;Rodulfo, Hectorina;Babar, Masroor E.;Imumorin, Ikhide G.
    • Journal of Animal Science and Technology
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    • v.60 no.12
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    • pp.32.1-32.10
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    • 2018
  • Background: Necdin (NDN), a member of the melanoma antigen family showing imprinted pattern of expression, has been implicated as causing Prader-Willi symptoms, and known to participate in cellular growth, cellular migration and differentiation. The region where NDN is located has been associated to QTLs affecting reproduction and early growth in cattle, but location and functional analysis of the molecular mechanisms have not been established. Methods: Here we report the sequence variation of the entire coding sequence from 72 samples of cattle, yak, buffalo, goat and sheep, and discuss its variation in Bovidae. Median-joining network analysis was used to analyze the variation found in the species. Synonymous and non-synonymous substitution rates were determined for the analysis of all the polymorphic sites. Phylogenetic analysis were carried out among the species of Bovidae to reconstruct their relationships. Results: From the phylogenetic analysis with the consensus sequences of the studied Bovidae species, we found that only 11 of the 26 nucleotide changes that differentiate them produced amino acid changes. All the SNPs found in the cattle breeds were novel and showed similar percentages of nucleotides with non-synonymous substitutions at the N-terminal, MHD and C-terminal (12.3, 12.8 and 12.5%, respectively), and were much higher than the percentage of synonymous substitutions (2.5, 2.6 and 4.9%, respectively). Three mutations in cattle and one in sheep, detected in heterozygous individuals were predicted to be deleterious. Additionally, the analysis of the biochemical characteristics in the most common form of the proteins in each species show very little difference in molecular weight, pI, net charge, instability index, aliphatic index and GRAVY (Table 4) in the Bovidae species, except for sheep, which had a higher molecular weight, instability index and GRAVY. Conclusions: There is sufficient variation in this gene within and among the studied species, and because NDN carry key functions in the organism, it can have effects in economically important traits in the production of these species. NDN sequence is phylogenetically informative in this group, thus we propose this gene as a phylogenetic marker to study the evolution and conservation in Bovidae.

A Study on the Location and Spatial Organization Characteristics of the Royal Tombs Uireung (의릉(懿陵) 일원(一圓)의 입지(立地)와 공간구성특성(空間構成特性)에 관(關)한 연구(硏究))

  • Choi, Jong Hee;Kim, Heung Nyeon;Lee, Won;Eom, Tae Geon
    • Korean Journal of Heritage: History & Science
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    • v.43 no.1
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    • pp.212-235
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    • 2010
  • The purpose of this study is to examine the characteristics of the location and the spatial composition of Uireung that is located in Seokgwan-dong, Seongbuk-gu, Seoul, in order to understand the landscape architectural characteristics. The results are as follows. First, Uireung is 6.4km from Changdeokgung Palace and 5.5km from Heunginjimun Gate. It did not violate the distance standard (40km) for the royal tombs according to Joseon Dynasty Neung-won Myo-je. Second, Uireung is in harmony with the nature and shows the authoritative characteristics of the royal authority through the spatial composition and rank(Entrance Area, Ceremonial Area, Burial Area). Third, there are burial mound, stone sheep, stone tiger, stone table, stone watch pillars in the upper platform, and stone civil official, stone horse, stone lantern in the middle platform, and stone military official, stone horse in the lower platform, and T-shape shrine, worship road in the ceremonial area. There is no pond and a tomb keeper residence, but the position, size, and form can be approximated through historical research materials. There are a colony of pine trees around the burial mound and 64 species of trees such as pine tree, zelcova tree, and fir tree below the burial mound.

A Study on the Data Cleaning and Standardization of National Ecosystem Survey in Korea (전국자연환경조사 데이터 정제와 표준화 방안 연구)

  • Kwon, Yong-Su;Song, Kyohong;Kim, Mokyoung;Kim, Kidong
    • Korean Journal of Ecology and Environment
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    • v.53 no.4
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    • pp.380-389
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    • 2020
  • Research on diagnosing and predicting the response of ecosystems caused by environmental changes such as artificial disturbance and climate change is emerging as the most important issue of biodiversity and ecosystem researches. This study aims to clean, standardize, and provide the results of National Ecosystem Survey which should be considered fundamentally in diagnosing and predicting ecosystem changes in the form of dataset. To refine and clean the dataset we developed a simple verification program based on the fifth National Ecosystem Survey Guideline and applied that program to the data from the second (1997~2005), third (2006~2013) and fourth (2014~2018) National Ecosystem Survey. Data quality control processes were implemented including (1) standardization of terminology, (2) similar data table integration, (3) unnecessary attribute and error elimination, (4) unification of different input items, (5) data arrangement in codes, and (6) code mapping for input items. These approaches and methods are the first attempt propose an option for ecological data standardization in Korea. The standardized dataset of National Ecosystem Survey in Korea will be easily accessible, reusable for both researchers and public. In addition, we expect it will contribute to the establishment of diverse environmental policies concerning environmental assessments, habitat conservation, prediction of endangered species distribution and ecological risks due to climate change. The dataset through this study is open freely online via EcoBank (nie-ecobank.kr) which is the first ecological information portal system in Korea developed by National Institute of Ecology.

Experimental Study on the Reological Properties of Carbon Nano Materials as Cement Composites (탄소계 나노소재를 적용한 시멘트 페이스트 복합체의 유변학적 특성에 대한 연구)

  • Kim, Won-Woo;Moon, Jae-Heum;Yang, Keun-Hyeok
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.10 no.3
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    • pp.227-234
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    • 2022
  • In this study, the rheological properties of cement paste composites applied with carbon-based nano-materials were experimental analyzed. Flow table and rheological properties, compressive strength were measured in the cement paste using graphene oxide asqueous solution and carbon nanotube aqueous solution. When carbon nano-materials was mixed in an aqueous solution, flow decreased and plastic viscosity and shear stress were increased. In particular, graphene oxide rapidly increased the plastic viscosity and shear stress. In the case of carbon nanotube aqueous solution, when less than 0.2 % was mixed, the increase rate was low compared to graphene oxide. This is because the specific surface area of graphene, which is in the form of a plate, is large. The compressive strength showed a small amount in strength increase when graphene mix, and CNT had a strength about 112 % of OPC. Carbon-based nanomaterials, is considered that CNT are suitable more to be used construction materials. However, extra studies on the surfactant to be used for mixing proportion and dispersion will be needed.

Data Product Value Evaluation Method for Data Exchange Platform (데이터거래 활성화를 위한 데이터상품가치 평가모델 연구)

  • Kim, Sujin;Lee, Junghyun;Park, Cheonwoong
    • The Journal of the Korea Contents Association
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    • v.21 no.12
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    • pp.34-46
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    • 2021
  • In the domestic data exchanging market, unreasonable pricing of purchase data is consistently mentioned as a major obstacle in data trading. This is a problem caused by the inability to properly evaluate the value of data products due to lack of product information and experience in using them. In order to activate trading, the data exchanges need to provide information that allows consumers to comprehensively judge the value of data products in addition to prices. The cost-based, income-based, and market-based methods, which are mainly applied to data valuation, are insufficient as data valuation methods to stimulate trading and distribution because only price information, a result of valuation from a supplier's point of view, can be shared with consumers. This study aims to develop a measurable valuation method that allows data trading stakeholders (exchanges, suppliers, and consumers) to judge and share the value of data products from a common perspective. To this end, we identified the value drivers of data products, which are considered important in overseas data exchanges and related research, and derived an evaluation method that can quantitatively measure each value driver. In addition, evaluation criteria in the form of a rating table were developed using data products for transactions, and a value evaluation index was developed through stratification analysis (AHP) to enable relative value comparison. As a result of applying the evaluation criteria to actual data products, it was found that the evaluation values were differentiated according to the characteristics of individual data products, so it could be used as a relative value comparison tool.

Vegetation Classification and Ecological Characteristics of Black Locust (Robinia pseudoacacia L.) Plantations in Gyeongbuk Province, Korea (경북지방 아까시나무 조림지의 식생유형과 생태적 특성)

  • Jae-Soon Song;Hak-Yun Kim;Jun-Soo Kim;Seung-Hwan Oh;Hyun-Je Cho
    • Journal of Korean Society of Forest Science
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    • v.112 no.1
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    • pp.11-22
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    • 2023
  • This study was established to provide basic information necessary for ecological management to restore the naturalness of black locust (Robinia pseudoacacia L.) plantations located in the mountains of Gyeongbuk, Korea. Using vegetation data collected from 200 black locust stands, vegetation types were classified using the TWINSPAN method, the spatial arrangement status according to the environmental gradient was identified through DCA analysis, and a synoptic table of communities was prepared based on the diagnostic species determined by determining community fidelity (Φ) for each vegetation type. The vegetation types were classified into seven types, namely, Quercus mongolica-Polygonatum odoratum var. pluriflorum type, Castanea crenata-Smilax china type, Clematis apiifolia-Lonicera japonica type, Rosa multiflora-Artemisia indica type, Quercus variabilis-Lindera glauca type, Ulmus parvifolia-Celtis sinensis type, and Prunus padus-Celastrus flagellaris type. These types usually reflected differences in complex factors such as altitude, moisture regime, successional stage, and disturbance regime. The mean relative importance value of the constituent species was highest for black locust(39.7), but oaks such as Quercus variabilis, Q. serrata, Q. mongolica, Q. acutissima, and Q. aliena were also identified as important constituent species with high relative importance values, indicating their potential for successional trends. In addition, the total percent cover of constituent species by vegetation type, life form composition, species diversity index, and indicator species were compared.

Development of Smartphone Application for Cognitive Behavioral Therapy-Based Case Management in Patients with Schizophrenia (조현병 환자의 인지행동치료 기반 사례관리를 위한 스마트폰 애플리케이션 개발)

  • Kim, Sung-Wan;Lee, Ga-Young;Yu, Hye-Young;Park, Ji-Hyun;Lee, Yong-Sung;Kim, Ju-Wan;Park, Cheol;Lee, Ju-Yeon;Lee, Yo-Han;Kim, Jae-Min;Yoon, Jin-Sang
    • Korean Journal of Schizophrenia Research
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    • v.19 no.1
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    • pp.10-16
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    • 2016
  • Objectives : This article aims to describe the development of smartphone application for the case management of patients with schizophrenia. Methods : Gwangju Bukgu-Community Mental Health Center developed and launched a smartphone application (HYM) for cognitive-behavioral case management and symptom monitoring. The development of the application involved psychiatrists, nurses, social workers, psychologists, and software technicians from a software development company (Goosl Corp.). Results : The HYM application for clients includes six main modules including Thought record, Symptom record, Daily life record, Official notices, Communication, and Scales. The key module is the 'Thought Record' for self-directed cognitive-behavioral treatment (CBT). When the client writes and sends the self-CBT sheet to the case manager, the latter receives a notification and can provide feedback in real time. 'Communication' and 'Official notices' are useful for promoting communication between case managers and clients with schizophrenia. Ratings in 'Symptom record', 'Daily life record', and 'Scales' modules are stored in graphic or table form representing changes in them and shared with case managers. Conclusion : The interactive function of this application is the key characteristics that distinguishes it from other mobile self-treatment tools. This smartphone application may contribute to the development of a youth- and customer-friendly case management system for individuals with early psychosis.

Draft List and Relative Importance of Principal Processes in the Geosphere to be Considered for the Radiological Safety Assessment of the Domestic Geological Disposal Facility through Analyzing FEPs for KBS-3 Type Disposal Repository of High-level Radioactive Waste(HLW) (KBS-3 방식 고준위방폐물 심층처분장 FEP 분석을 통한 국내 사용후핵연료 심층처분시설 방사선학적 안전성 평가용 지권영역 주요 프로세스 항목 및 상대적 중요도 도출)

  • Sukhoon Kim;Donghyun Lee;Dong-Keuk Park
    • Journal of Radiation Industry
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    • v.17 no.1
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    • pp.33-44
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    • 2023
  • The deep geological repository of high-level radioactive waste shall be designed to meet the safety objective set in the form of radiation dose or corresponding risk to protect human and the environment from radiation exposure. Engineering feasibility and conformity with the safety objective of the facility conceptual design can be demonstrated by comparing the assessment result using the computational model for scenario(s) describing the radionuclide release and transport from repository to biosphere system. In this study, as the preliminary study for developing the high-level radioactive waste disposal facility in Korea, we reviewed and analyzed the entire list of FEPs and how to handle each FEP from a general point of view, which are selected for the geosphere region in the radiological safety assessment performed for the license application of the KBS-3 type deep geological repository in Finland and Sweden. In Finland, five FEPs (i.e., stress redistribution, creep, stress redistribution, erosion and sedimentation in fractures, methane hydrate formation, and salt exclusion) were excluded or ignored in the radionuclide release and transport assessment. And, in Sweden, six FEPs (i.e., creep, surface weathering and erosion, erosion/sedimentation in fractures, methane hydrate formation, radiation effects (rock and grout), and earth current) were not considered for all time frames and earthquake out of a total of 25 FEPs for the geosphere. Based on these results, an FEP list (draft) for the geosphere was derived, and the relative importance of each item was evaluated for conducting the radiological safety assessment of the domestic deep geological disposal facility. Since most of information on the disposal facility in Korea has not been determined as of now, it is judged that all FEP items presented in Table 3 should be considered for the radiological safety assessment, and the relative importance derived from this study can be used in determining whether to apply each item in the future.

An Analytical Study on the Stem-Growth by the Principal Component and Canonical Correlation Analyses (주성분(主成分) 및 정준상관분석(正準相關分析)에 의(依)한 수간성장(樹幹成長) 해석(解析)에 관(關)하여)

  • Lee, Kwang Nam
    • Journal of Korean Society of Forest Science
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    • v.70 no.1
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    • pp.7-16
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    • 1985
  • To grasp canonical correlations, their related backgrounds in various growth factors of stem, the characteristics of stem by synthetical dispersion analysis, principal component analysis and canonical correlation analysis as optimum method were applied to Larix leptolepis. The results are as follows; 1) There were high or low correlation among all factors (height ($x_1$), clear height ($x_2$), form height ($x_3$), breast height diameter (D. B. H.: $x_4$), mid diameter ($x_5$), crown diameter ($x_6$) and stem volume ($x_7$)) except normal form factor ($x_8$). Especially stem volume showed high correlation with the D.B.H., height, mid diameter (cf. table 1). 3) (1) Canonical correlation coefficients and canonical variate between stem volume and composite variate of various height growth factors ($x_1$, $x_2$ and $x_3$) are ${\gamma}_{u1,v1}=0.82980^{**}$, $\{u_1=1.00000x_7\\v_1=1.08323x_1-0.04299x_2-0.07080x_3$. (2) Those of stem volume and composite variate of various diameter growth factors ($x_4$, $x_5$ and $x_6$) are ${\gamma}_{u1,v1}=0.98198^{**}$, $\{{u_1=1.00000x_7\\v_1=0.86433x_4+0.11996x_5+0.02917x_6$. (3) And canonical correlation between stem volume and composite variate of six factors including various heights and diameters are ${\gamma}_{u1,v1}=0.98700^{**}$, $\{^u_1=1.00000x_7\\v1=0.12948x_1+0.00291x_2+0.03076x_3+0.76707x_4+0.09107x_5+0.02576x_6$. All the cases showed the high canonical correlation. Height in the case of (1), D.B.H. in that of (2), and the D.B.H, and height in that of (3) respectively make an absolute contribution to the canonical correlation. Synthetical characteristics of each qualitative growth are largely affected by each factor. Especially in the case of (3) the influence by the D.B.H. is the most significant in the above six factors (cf. table 2). 3) Canonical correlation coefficient and canonical variate between composite variate of various height growth factors and that of the various diameter factors are ${\gamma}_{u1,v1}=0.78556^{**}$, $\{u_1=1.20569x_1-0.04444x_2-0.21696x_3\\v_1=1.09571x_4-0.14076x_5+0.05285x_6$. As shown in the above facts, only height and D.B.H. affected considerably to the canonical correlation. Thus, it was revealed that the synthetical characteristics of height growth was determined by height and those of the growth in thickness by D.B.H., respectively (cf. table 2). 4) Synthetical characteristics (1st-3rd principal component) derived from eight growth factors of stem, on the basis of 85% accumulated proportion aimed, are as follows; Ist principal component ($z_1$): $Z_1=0.40192x_1+0.23693x_2+0.37047x_3+0.41745x_4+0.41629x_5+0.33454x_60.42798x_7+0.04923x_8$, 2nd principal component ($z_2$): $z_2=-0.09306x_1-0.34707x_2+0.08372x_3-0.03239x_4+0.11152x_5+0.00012x_6+0.02407x_7+0.92185x_8$, 3rd principal component ($z_3$): $Z_3=0.19832x_1+0.68210x_2+0.35824x_3-0.22522x_4-0.20876x_5-0.42373x_6-0.15055x_7+0.26562x_8$. The first principal component ($z_1$) as a "size factor" showed the high information absorption power with 63.26% (proportion), and its principal component score is determined by stem volume, D.B.H., mid diameter and height, which have considerably high factor loading. The second principal component ($z_2$) is the "shape factor" which indicates cubic similarity of the stem and its score is formed under the absolute influence of normal form factor. The third principal component ($z_3$) is the "shape factor" which shows the degree of thickness and length of stem. These three principal components have the satisfactory information absorption power with 88.36% of the accumulated percentage. variance (cf. table 3). 5) Thus the principal component and canonical correlation analyses could be applied to the field of forest measurement, judgement of site qualities, management diagnoses for the forest management and the forest products industries, and the other fields which require the assessment of synthetical characteristics.

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A Model to Measure the Success of a Web-based Information System at a Government Agency - the Chungwadae Case (공공기관 업무관리시스템 성과평가 모형 개발에 관한 연구: 청와대 업무관리시스템(e지원시스템)을 중심으로)

  • Bae, Lee-Chul;Hong, Il-Yoo
    • Asia pacific journal of information systems
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    • v.18 no.1
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    • pp.97-115
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    • 2008
  • Introduction The e-government is concerned with using Internet and Web technologies to exchange information and services with citizens, businesses and other related organizations, and it centers on three functions, namely informational, interactive, and transactional [UN, 2001]. Many developed countries like the U.S. have been actively involved in e-government projects, since they enable both more effective public services for citizens and more efficient internal operations. Korea is among these leading countries that are planning to leverage computer and communication technologies to provide for integration of work processes and information as well as convenient access to information and services. For this reason, evaluating e-government projects is becoming a crucial issue for both researchers and policy-makers. However, most research to date has primarily focused on a model of success of an e-government system designed for citizens, overlooking internal systems specifically created for employees working in a public organization. This paper is intended to propose a model to measure the success of a Web-based information system designed for use by internal users at Chungwadae, the executive branch of Korea's central government. The paper is also aimed at applying the model to the assessment of the present system being used at Chungwadae in comparison with the preceding system. Evaluating an e-Government System The most widely cited model of information systems success today is that of DeLone and McLean[1992, 2003, 2004]. The original model states that the success of an information system can be measured using six dimensions, including system quality, information quality, use, user satisfaction, individual impact, and organizational impact. Although the ultimate success of an information system may be reflected in the impact that the system has upon individuals as well as an organization, aspects of using the system such as system use and user satisfaction can play an important role in determining the system success, because the system would be a sheer failure if users don't like and use the system. As a response to criticisms given by numerous researchers, the authors adapted their model to fit the emerging Web-based environment. The revised model[DeLone and McLean, 2003] they offered included an additional quality dimension, namely service quality, and combined individual and organizational impacts into net benefits which can also influence user satisfaction. The e-government system success model can be built around this updated model. Our model incorporates information quality, system quality, and service quality as in the DeLone and McLean model. However, the 'system use' dimension has been replaced by perceived usefulness, as suggested by Seddon[1998]. In addition, because the e-government systems that this paper focuses on are internal public systems used in government agencies, the 'net benefits' dimension has been replaced by perceived work efficiency. Based on the proposed model, a total of nine hypotheses have been formulated which we tested using an empirical analysis. Methods A questionnaire form has been created with items that are designed to examine the relationships among the variables in the model. The questionnaire has been handed over, in person, to 65 members of Chungwadae staff who are now actively using the E-Support System, the present information system created to support internal work at Chungwadae. We made arrangements to meet with each individual who agreed to participate in our survey, and helped to fill out the survey form with explanations. Of the 65 copies that were delivered, only 33 were returned, and 30 responses of these have been adopted for our analysis, since three were not valid. The extremely small sample size was due to the limited number of staff members who had adequate experience required of this study. Results We gathered data from the questionnaire survey and analyzed them using a regression analysis to test the hypotheses. As shown in the table below, the results indicated that all three dimensions of an information system’s quality are positively related to user satisfaction. However, information quality and system quality were found to be positively related to perceived usefulness, while service quality was not. In addition, perceived usefulness is not positively related to user satisfaction, implying that a user may find a system useful, but may not be satisfied with it. Finally, user satisfaction and perceived usefulness both are positively related to perceived work efficiency. This suggests that workers' positive experience with the system is important to guarantee favorable work efficiency. Conclusions We conclude that the proposed model proved useful in measuring the success of an internal information system used by a government agency. To demonstrate the applicability and usefulness of the model in the paper, we applied the model to the assessment of the present internal system used at Chungwadae in comparison with the preceding system. The results showed that the present system outperforms the preceding one in a statistically significant way. Future research will have to focus on applying the model to Korea's governmental agencies other than Chungwadae and examine whether it proves applicable in different types of governmental organizations.