• Molecules and Cells
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• 제44권9호
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• pp.647-657
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• 2021

As a pancreatic inflammatory marker, regenerating islet-derived protein 3A (Reg3A) plays a key role in inflammation-associated pancreatic carcinogenesis by promoting cell proliferation, inhibiting apoptosis, and regulating cancer cell migration and invasion. This study aimed to reveal a novel immuno-regulatory mechanism by which Reg3A modulates tumour-promoting responses during pancreatic cancer (PC) progression. In an in vitro Transwell system that allowed the direct co-culture of human peripheral blood-derived dendritic cells (DCs) and Reg3A-overexpressing/ silenced human PC cells, PC cell-derived Reg3A was found to downregulate CD80, CD83 and CD86 expression on educated DCs, increase DC endocytic function, inhibit DC-induced T lymphocyte proliferation, reduce IL-12p70 production, and enhance IL-23 production by DCs. The positive effect of tumour-derived Reg3A-educated human DCs on PC progression was demonstrated in vivo by intraperitoneally transferring them into PC-implanted severe combined immunodeficiency (SCID) mice reconstituted with human T cells. A Reg3A-JAK2/STAT3 positive feedback loop was identified in DCs educated with Reg3A. In conclusion, as a tumour-derived factor, Reg3A acted to block the differentiation and maturation of the most important antigen-presenting cells, DCs, causing them to limit their potential anti-tumour responses, thus facilitating PC escape and progression.

• 한국항행학회논문지
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• 제14권6호
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• pp.817-823
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• 2010

본 논문에서는 휴대전화 단말기 내장형 안테나인 IFA에 IPI를 채택하여 이득이 증가함을 보인다. T 정합 이론을 근거로 하는 IPI 급전 구조는 IFA와 같은 내장형 안테나의 복사 저항을 작게 하므로 안테나에 흐르는 전류를 상대적으로 증가시켜 복사 효율과 이득 향상을 가져온다. 역 파이 급전 이론의 타당성을 밝히기 위하여 IFA에 IPI 급전 구조를 설계 적용하여 제반 특성을 측정하였다. CDMA/DCS 주파수 대역에서 IPI-IFA는 공진 주파수의 이동 없이 IFA보다 이득이 0.5 ~ 1.4[dBi] 정도 향상됨을 보였다.

• IMMUNE NETWORK
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• 제16권4호
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• pp.233-241
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• 2016

DCs, like the sensory neurons, express vanilloid receptor 1 (VR1). Here we demonstrate that the VR1 agonists, capsaicin (CP) and resiniferatoxin (RTX), enhance antiviral CTL responses by increasing MHC class I-restricted viral antigen presentation in dendritic cells (DCs). Bone marrow-derived DCs (BM-DCs) were infected with a recombinant vaccinia virus (VV) expressing OVA (VV-OVA), and then treated with CP or RTX. Both CP and RTX increased MHC class I-restricted presentation of virus-encoded endogenous OVA in BM-DCs. Oral administration of CP or RTX significantly increased MHC class I-restricted OVA presentation by splenic and lymph node DCs in VV-OVA-infected mice, as assessed by directly measuring OVA peptide SIINFEKL-K^b complexes on the cell surface and by performing functional assays using OVA-specific CD8 T cells. Accordingly, oral administration of CP or RTX elicited potent OVA-specific CTL activity in VV-OVA-infected mice. The results from this study demonstrate that VR1 agonists enhance anti-viral CTL responses, as well as a neuro-immune connection in anti-viral immune responses.

• 한국산학기술학회논문지
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• 제15권11호
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• pp.6767-6773
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• 2014

본 연구는 고유수용성신경근촉진법의 체간 패턴 운동을 병행한 경두개직류자극이 뇌졸중 환자의 하지 근 활성도와 균형 능력, 보행 속도에 어떠한 영향을 미치는지 알아보기 위하여 실시하였다. 본 연구에는 총 31명의 뇌졸중 환자가 참여하였으며, 대상자들은 무작위 추출법을 통해서 실험군 15명과 대조군 16명으로 각각 배정되었다. 모든 대상자들은 고유수용성 신경근촉진법의 체간 패턴 운동을 6주 동안, 주 3회 20분 씩 시행하였고, 실험군에는 추가적으로 경두개직류자극을 20분 간 시행하였다. 연구 결과 실험군이 대조군에 비해 마비측 하지의 비복근, 전경골근의 활성도와 균형 능력에서 유의한 차이를 보였다(p<.05). 이러한 결과는 고유수용성신경근촉진법의 체간 패턴 운동을 병행한 경두개직류자극이 뇌졸중 환자의 하지 기능 회복에 긍정적인 효과를 가져다 준 것으로 사료된다.

• 대한의용생체공학회:의공학회지
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• 제32권4호
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• pp.305-311
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• 2011

For effective stimulation with tDCS, spatial focality of induced electrical field(EF) and current density(CD) is one of the important factors to be considered. Recently, there have been some studies to improve the spatial focality via different types of electrodes and their new configurations: some improvements using ring electrodes were reported over the conventional pad electrodes. However, most of these studies assumed isotropic conductivities in the head. In this work, we have investigated the effect of tissue anisotropy on the spatial focality of tDCS with the 4 + 1 ring electrode configuration via a 3-D high-resolution finite element(FE) head model with anisotropic conductivities in the skull and white matter. By examining the profiles of the induced EF from the head models with isotropic and anisotropic conductivities respectively, we found that the spatial focality of the induced EF significantly drops and get diffused due to tissue anisotropy. Our analysis suggests that it is critical to incorporate tissue anisotropy in the effective stimulation of the brain via tDCS.

• 한국전자파학회논문지
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• 제18권12호
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• pp.1337-1344
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• 2007

이동 통신 단말기 내장형 안테나의 급전 부분에 역 파이 형태의 급전부를 채택함으로써 이득이 증가함을 보인다. PIFA, IFA 등과 같은 안테나 급전 부분을 수정하여 안테나와 같은 방향으로 전류가 흐를 수 있도록 하면 T 정합 이론을 적용할 수 있다. 따라서 T 정합 이론을 근거로 하는 역 파이 급전은 안테나의 방사 저항을 높이고 더불어 이득도 증가시킬 수 있다. 역 파이 급전 이론의 타당성을 밝히기 위하여 기존의 GSM/ DCS 2중 대역 PIFA에 적용하여 제반 특성을 측정한다. 측정 결과, 공진 주파수의 이동은 없으며, 채널별 평균 이득은 역 파이 급전 PIFA가 기존 PIFA에 비해 GSM 대역에서는 $0.23{\sim}0.84$ dB, DCS 대역에서는 $0.01{\sim}1.74$ dB 향상됨을 보인다.

• 국제물리치료학회지
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• 제4권1호
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• pp.479-487
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• 2013

This study is intended to examine the tDCS and Morris Water maze training in Alzheimer's disease(AD) rats on Tau protein expression. Experiment groups were divided into four groups and assigned 16 rats to each group. Group I was a control group(AD induced by scopolamine); Group II was a experimental control group(AD injured by scopolamine and treatment tacrine); Group III was a group of tDCS application after AD injured by scopolamine; Group IV was a group of morris water maze training after AD injured by scopolamine. In cognition test, the outcome of group II was significantly lower than the groups(p<.001). and group III, IV were significantly low result at 14 days(p<.05). In histological finding, the experimental groups were destroy of micro vessels and finding of cell atropy and swelling. Group III, IV were decreased in degeneration of liver and kidney cells. In immuno- histochemistric response of BDNF and tau protein in hippocampus, BDNF expression of Group II was more increase than the other groups. and increase of BDNF expression was III, IV were higher than group I at 21 days. Tau protein expression of Group II was more decrease than the other groups. and decrease of Tau protein expression was III, IV were lower than group I at 21 days. These result suggest that improved tDCS and morris water maze training after scopolamine induced is associated with dynamically altered expression of BDNF and Tau protein in hippocampus and that is related with cognitive function.

• 대한통합의학회지
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• 제7권1호
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• pp.9-17
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• 2019

Purpose : This study assessed the effects of transcranial direct current stimulation (tDCS) on balance, fall efficacy, and fall-related fitness in stroke patients, using a cohort of 30 stroke patients divided into two groups. Methods : The experimental group (was given transcranial direct current stimulation in a virtual reality program) and the control group was given false tDCS in virtual reality. there were 15 patients in each group, receiving appropriate treatment over 30 sessions (30 minutes per session per week over a six-week period). In order to assess the change in balance before and after the intervention, the Berg Balance scale was utilized. Fall efficacy was evaluated using the Korean Falls Efficacy Scale for the Elderly (FES-K), The following exercises were performed by patients to assess fall-related fitness : sitting down in a chair and standing up : walking a 244 cm round= trip, and standing on one foot. Results : After the intervention, the experimental group exhibited significantly increased fall efficacy and fall-related fitness, while the control group exhibited no change. These findings suggest that tDCS has positive effects on balance, fall efficacy, and fall-related fitness in stroke patients. Conclusion : Using tDCS as an intervention would bring positive effects on balance, fall efficacy, and fall-related fitness in stroke patients undergoing rehabilitation.

• IMMUNE NETWORK
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• 제14권4호
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• pp.207-218
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• 2014

Chronic virus infection leads to the functional impairment of dendritic cells (DCs) as well as T cells, limiting the clinical usefulness of DC-based therapeutic vaccine against chronic virus infection. Meanwhile, B cells have been known to maintain the ability to differentiate plasma cells producing antibodies even during chronic virus infection. Previously, ${\alpha}$-galactosylceramide (${\alpha}GC$) and cognate peptide-loaded B cells were comparable to DCs in priming peptide-specific $CD8^+$ T cells as antigen presenting cells (APCs). Here, we investigated whether B cells activated by ${\alpha}GC$ can improve virus-specific T cell immune responses instead of DCs during chronic virus infection. We found that comparable to B cells isolated from naïve mice, chronic B cells isolated from chronically infected mice with lymphocytic choriomeningitis virus (LCMV) clone 13 (CL13) after ${\alpha}GC$-loading could activate CD1d-restricted invariant natural killer T (iNKT) cells to produce effector cytokines and upregulate co-stimulatory molecules in both naïve and chronically infected mice. Similar to naïve B cells, chronic B cells efficiently primed LCMV glycoprotein (GP) 33-41-specific P14 $CD8^+$ T cells in vivo, thereby allowing the proliferation of functional $CD8^+$ T cells. Importantly, when ${\alpha}GC$ and cognate epitope-loaded chronic B cells were transferred into chronically infected mice, the mice showed a significant increase in the population of epitope-specific $CD8^+$ T cells and the accelerated control of viremia. Therefore, our studies demonstrate that reciprocal activation between ${\alpha}GC$-loaded chronic B cells and iNKT cells can strengthen virus-specific T cell immune responses, providing an effective regimen of autologous B cell-based therapeutic vaccine to treat chronic virus infection.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.130.1-130.1
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• 2003

As a potent antigen presenting cells and a powerful inducer of antigen specific immunity including cytotoxic T cell activity, dendritic cells(DCs) are being considered as a promising anti-tumor therapeutic module. Unlike solid tumors, leukemia is the hematologic malignancy involving immune effector cells. The expected usage of DCs in leukemia is the treatment of minimal residual disease(MRD) after the remission or stem cell transplantation. In this study, syngeneic leukemia cells were inoculated intra-venously into the mouse (WEHI-3 into the Balb/c), and the autologous tumor cell lysate pulsed DCs were injected as a therapeutic module twice in two weeks. (omitted)