• BMB Reports
• /
• 제42권8호
• /
• pp.511-515
• /
• 2009

Downstream of Bid (DOBI) known as Pus10, has been identified as a modulator of TRAIL-induced cell death using RNAi library screening. The crystal structure of DOBI has revealed that it is a crescent-shaped protein containing the pseudouridine synthase catalytic domain and a THUMP-containing domain. Here, we demonstrated that DOBI is expressed in various tissues such as heart and lung, and is also expressed in various tumor cells such as HeLa and A549. Although ectopic expression of DOBI does not promote TRAIL death signaling in HeLa cells, knock-down of DOBI expression using shRNA inhibited TRAIL death signaling. DOBI is cleaved into a 54 kD cleaved DOBI during cell death, and the recombinant DOBI protein can be directly cleaved by caspases-3, or -8 in vitro. Together, these data suggest that the cleaved DOBI may acquire a new function, possibly by cooperating with tBid in the mitochondrial event of cell death caused by TRAIL.

• 생명과학회지
• /
• 제27권11호
• /
• pp.1340-1348
• /
• 2017

Sanguinarine은 다양한 목적으로 사용되고 있는 Sanguinaria canadensis L.의 뿌리에서 유래된 benzophenanthridine alkaloid 계열 물질중의 하나이다. 그동안 sanguinarine의 다양한 약리학적인 효능이 알려져 왔고, 항암활성에 대한 연구도 여러 암세포들을 대상으로 수행되어 왔다. 그러나 sanguinarine에 의한 암세포의 apoptosis 유도에 대한 현상은 여전히 많은 부분에서 연구의 대상으로 남아 있다. 본 연구는 Hep3B 인체 간암세포를 대상으로 sanguinarine의 항암활성에 대한 추가적인 자료를 제시하기 위하여 수행되었다. 본 논문의 결과에 의하면, sanguinarine은 처리 농도 의존적으로 Hep3B 세포의 증식을 억제하였으며, 이는 apoptosis 유도와 연관성이 있었다. Sanguinarine은 두 가지 apoptosis 경로인 extrinsic 및 intrinsic 경로의 개시 initiator caspase인 caspase-8 및 caspase-9 뿐만 아니라 대표적인 effector caspase인 caspase-3의 활성을 증가시켰고, caspase-3의 기질인 PARP의 분절을 유발하였다. 아울러 sanguinarine은 DR-related 유전자들의 발현을 부분적으로 증가시켰으며, Bcl-2 family에 속하는 pro-apoptotic Bax의 발현을 증가시킨 반면, anti-apoptotic Bcl-2의 발현은 억제시켰다. 또한 sanguinarine은 Bid의 truncation을 촉진하였고, MMP의 소실에 따른 cytochrome c를 미토콘드리아에서 세포질로의 이동을 증가시켰다. 그리고 sanguinarine에 의한 apoptosis 유도 및 세포 증식율 억제 현상이 caspase의 활성을 인위적으로 억제하였을 경우, 모두 사라졌다. 따라서 sanguinarine에 의하여 유도하는 Hep3B 세포의 apoptosis 유발에는 caspase 의존적으로 extrinsic 및 intrinsic 경로가 모두 관여하고 있음을 알 수 있었다.

• 한국임상수의학회지
• /
• 제24권2호
• /
• pp.225-228
• /
• 2007

개 추간판 디스크 2 증례(症例)를 대상으로 봉독약침 및 봉독약침과 한약제로 각각 치료하였다. 증례(症例) 1은 흉-요추부 추간판탈출증(T11-T12, T12-T13, L3-L4 및 L4-L5)으로 진단(診斷)된 증례이었으며. 증례 2는 흉추부 추간판탈출증(T10-T11 및 T12-T13)으로 진단(診斷)된 증례이었다. 이들 증례에 대하여 봉독약침(0.1 ml/혈위, 총$200{\mu}g$)을 실시(實施)하였으며, 운동요법과 수영요법도 병용하였다. 봉독약침에 사용한 혈위는 GV20-Bai Hui, GB30-Huan Tiao, ST36-Zu San Li, GB34-Yang Ling Quan, ST40-Feng Long, ST41-Jie Xi 및 BL40-Wei Zhong, 병변부 및 압통점이었다. 또한 증례 2에 대하여는 봉독약침과 더불어 Zheng Gu Zi Jin Dan(정골자금단(正骨紫金丹) : 1 g), Shiuh Duann(속단(續斷) .0.2 g), Du Zhong(두중(杜仲) : 0.2 g), Mo Yao(몰약(沒藥 : 0.2 g), Ru Xiang(유향(乳香) : 0.2 g) 및 Pyrite(자연동(自然銅) : 0.2 g)를 각각 총 9일간 경구투여(2회/1일)하였다. 증례 1은 총 4주간 11회 치료, 증례(症例) 2는 총 2주간 6회 치료 후 각각 보행이 가능하였다.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 2004년도 하계학술대회 논문집 A
• /
• pp.737-739
• /
• 2004

The domestic power system established with Cost-Based-Pricing(CBP) from April 2001. The system is a uniform pricing system. System Operator(50) establishes a Price Setting Schedule by the prediction of consumption and the presented bid price(generation cost) of the generation utility. But the Price Setting Schedule doesn't take account of the constraint of the system. This cause a transmission congestion, constrained-on generation and constrained-off generation. This Paper search the way of the increasing efficiency of domestic power system through the redemption of congestion charge.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 2001년도 추계학술대회 논문집 전력기술부문
• /
• pp.170-172
• /
• 2001

This paper describes the settlement rules in the basic market design for Korean Wholesale Electricity Market. The settlement rules are to be exercised after the Bid and Offer Processes as described in the pool rules(within the market rules) and to determine the payments to Generators, charges to Energy Purchasers and charges or payments to the Transmission Asset Owner(TAO).

• 약학회지
• /
• 제45권6호
• /
• pp.664-669
• /
• 2001

Acebutolol is almost absorbed after oral administration, but its bioavailability is reduced because of considerable first-pass metabolism through the gastrointestinal tract and liver. The purpose of this study was to report the pharmacokinetic changes of acebutolol (15 mg/kg,oral) and its main metabolite, diacetolol in rabbits pretreated (15 mg/kg, oral) and coadministered (15 mg/kg, S. C., bid for 3 days) with diltiazem. The plasma concentration and area under the plasma concentration-time curves (AUC) of acebutolol and diacetolol were significantly increased in rabbits pretreated and coadministered with diltazem. The elimination rate constant ( $K_{el}$ ) and total body clearances (CL $_{t}$) of acebutolol and diacetolol were significantly decreased and half-life of those were significantly prolonged in the rabbit. Metabolite percentage rate of diacetolol to the plasma concentration of total acebutolol in rabbits pretreated and coadministered with diltiazem were significantly decreased. The results suggest that the dosage of acebutolol should be adjusted when the drug would be administered chronically with diltiazem in a clinical situation.n.

• 건축역사연구
• /
• 제19권6호
• /
• pp.173-186
• /
• 2010

This article is about the study on construction contractors in Daegu during the Japanese Colonial Rule. The first construction contractors who involved with Kyong-bu railroad work in 1904 personally. But, after they go through the bidding method, changed by the Japanese Government General of Korea in 1922, and the second bid rigging in 1932, contractors was changed gradually the organization into unlimited partnership, limited partnership or stock corporation. The number of them was increased. Most contractors were Japanese, and organized a limited partnership which has been capitalized at 30,000 won. On the other hand, Korean contractors couldn't work at an important part, except for the personal activities of Youngsil Lee(李永實). They were usually employed as consultants or field deputies by Japanese contractors. After the Liberation, Japanese construction contractors returned to Japan, however Tatuoka-kumi(龍岡組), Yasiro-kumi(屋代組) and Sakano-kumi(坂野組) that was leading the business which were took over by Koreans. They were leading architecture field in Daegu.

• 한국지진공학회논문집
• /
• 제20권1호
• /
• pp.33-43
• /
• 2016

This study investigates the performance of hollow precast segmental bridge columns with reinforcement details for material quantity reduction. The proposed triangular reinforcement details are economically feasible and rational, and facilitate shorter construction periods. The precast segmental bridge columns provides an alternative to current cast-in-place systems. We tested a model of hollow precast segmental bridge columns under a constant axial load and a quasi-static, cyclically reversed horizontal load. We used a computer program, Reinforced Concrete Analysis in Higher Evaluation System Technology (RCAHEST), for analysis of reinforced concrete structures. The used numerical method gives a realistic prediction of performance throughout the loading cycles for hollow precast segmental bridge column specimens investigated. As a result, proposed reinforcement details for material quantity reduction was equal to existing reinforcement details in terms of required performance.

• 한국정보처리학회논문지
• /
• 제6권2호
• /
• pp.459-466
• /
• 1999

I broadband ISDN every packet will show different result if it would be processed according to its usage by the server. That is, normal data won't show big differences if they would be processed at normal speed. But it will improve the quality of service to process some kinds of data - for example real time video or voice type data or some data for a bid to by something through the internet - more fast than the normal type data. solution for this problem was suggested - priority packets. But the analyses of them are under way. Son in this paper a switching system for an output queuing model in a single server was assumed and some packets were given priorities and analysed. And correlation, simulating real life situation, was given too. These packets were analysed through three cases, first packets having no correlation, second packets having only correlation and finally packets having priority three cases, first packets having no correlation, second packets having only correlation and finally packets having priority and correlation. The result showed that correlation doesn't affect the mean delay time and the high priority packets have improved mean delay time regardless of the arrival rate. Those packets were assumed to be fixed-sized like ATM fixed-sized cell and the contention strategy was assumed to be short bus contention method for the output queue, and the mean delay length and the maximum 버퍼 length not to lose any packets were analysed.

• Journal of Korean Neurosurgical Society
• /
• 제60권4호
• /
• pp.391-396
• /
• 2017

Objective : To investigate the neuroprotective effects of Ginkgolide B (GB) against ischemic stroke-induced injury in vivo and in vitro, and further explore the possible mechanisms concerned. Methods : Transient middle cerebral artery occlusion (tMCAO) mice and oxygen-glucose deprivation/reoxygenation (OGD/R)-treated N2a cells were used to explore the neuroprotective effects of GB. The expression of brain-derived neurotrophic factor (BDNF) was detected via Western blot and qRT-PCR. Results : GB treatment (4 mg/kg, i. p., bid) significantly reduced neurological deficits, water content, and cerebral infarct volume in tMCAO mice. GB also significantly increased Bcl-2/Bax ratio, reduced the expression of caspase-3, and protected against OGD/R-induced neuronal apoptosis. Meanwhile, GB caused the up-regulation of BDNF protein in vivo and in vitro. Conclusion : Our data suggest that GB might protect the brain against ischemic insult partly via modulating BDNF expression.