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http://dx.doi.org/10.3340/jkns.2016.1010.018

Ginkgolide B Modulates BDNF Expression in Acute Ischemic Stroke  

Wei, Hu (Department of Neurology, Affiliated Provincial Hospital of Anhui Medical University)
Sun, Tao (Department of Neurology, Nanjing General Hospital of Nanjing Command)
Tian, Yanghua (Department of Neurology, the First Hospital of Anhui Medical University)
Wang, Kai (Department of Neurology, the First Hospital of Anhui Medical University)
Publication Information
Journal of Korean Neurosurgical Society / v.60, no.4, 2017 , pp. 391-396 More about this Journal
Abstract
Objective : To investigate the neuroprotective effects of Ginkgolide B (GB) against ischemic stroke-induced injury in vivo and in vitro, and further explore the possible mechanisms concerned. Methods : Transient middle cerebral artery occlusion (tMCAO) mice and oxygen-glucose deprivation/reoxygenation (OGD/R)-treated N2a cells were used to explore the neuroprotective effects of GB. The expression of brain-derived neurotrophic factor (BDNF) was detected via Western blot and qRT-PCR. Results : GB treatment (4 mg/kg, i. p., bid) significantly reduced neurological deficits, water content, and cerebral infarct volume in tMCAO mice. GB also significantly increased Bcl-2/Bax ratio, reduced the expression of caspase-3, and protected against OGD/R-induced neuronal apoptosis. Meanwhile, GB caused the up-regulation of BDNF protein in vivo and in vitro. Conclusion : Our data suggest that GB might protect the brain against ischemic insult partly via modulating BDNF expression.
Keywords
Stroke; Ginkgolide B; Brain-derived neurotrophic factor; Apoptosis;
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