• BMB Reports
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• v.42 no.8
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• pp.511-515
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• 2009

Downstream of Bid (DOBI) known as Pus10, has been identified as a modulator of TRAIL-induced cell death using RNAi library screening. The crystal structure of DOBI has revealed that it is a crescent-shaped protein containing the pseudouridine synthase catalytic domain and a THUMP-containing domain. Here, we demonstrated that DOBI is expressed in various tissues such as heart and lung, and is also expressed in various tumor cells such as HeLa and A549. Although ectopic expression of DOBI does not promote TRAIL death signaling in HeLa cells, knock-down of DOBI expression using shRNA inhibited TRAIL death signaling. DOBI is cleaved into a 54 kD cleaved DOBI during cell death, and the recombinant DOBI protein can be directly cleaved by caspases-3, or -8 in vitro. Together, these data suggest that the cleaved DOBI may acquire a new function, possibly by cooperating with tBid in the mitochondrial event of cell death caused by TRAIL.

• Journal of Life Science
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• v.27 no.11
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• pp.1340-1348
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• 2017

Sanguinarine is a benzophenanthridine alkaloid derived from the roots of Sanguinaria canadensis L., which is used for the purpose of treating various diseases. Although studies of anticancer activities have been performed using various cancer cell lines, the phenomenon of inducing apoptosis in cancer cells by using sanguinarine requires more research. Therefore, this study investigated the anti-cancer activities and related mechanisms of sanguinarine used with Hep3B human hepatocellular carcinoma cells in terms of the regulation of apoptosis. Sanguinarine inhibited the proliferation of Hep3B cells in a concentration-dependent manner, which was associated with the induction of apoptosis. Sanguinarine also increased the activity of caspase-3, which is a typical effector caspase, and the activities of caspase-8 and caspase-9, which are key when initiating extrinsic and intrinsic apoptosis pathways, respectively. In addition, sanguinarine increased the expression of death receptor-related genes and pro-apoptotic BAX, which belongs to the Bcl-2 family, while suppressing the expression of anti-apoptotic Bcl-2. Sanguinarine promoted the truncation of Bid and enhanced the release of cytochrome c from the mitochondria to the cytoplasm due to a loss of mitochondrial membrane potential. Furthermore, the reduction of a survival rate that was induced by sanguinarine and the induction of apoptosis disappeared with the inhibition of artificial caspase activity. Therefore, the results of the study indicated that sanguinarine-induced apoptosis in Hep3B cells involves both extrinsic and intrinsic pathways; such apoptosis is a caspase-dependent phenomenon.

• Journal of Veterinary Clinics
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• v.24 no.2
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• pp.225-228
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• 2007

The therapy by injection-acupuncture (AP) with bee-venom (apitoxin) and injection-AP with apitoxin combined by administration of Chinese herbal medicine was applied in 2 cases with canine intervertebral disc disease (IVDD). Case 1 was diagnosed as thoraco-lumbar IVDD (T11-T12, T12-T13, L3-L4 and L4-L5) and case 2 was diagnosed as IVDD at T10-T11 and T12-T13, respectively Injection-AP with apitoxin($Apitoxinc{(R)}$, total $200{\mu}g$ of apitoxin, 0.1 ml/acupoint) plus physical exercise (walking with gocart, TID/day) and aquatherapy (swimming treatment, BID/week) were given to each patient. The used acupoints were GV20 (Bai Hui), GB30 (Huan Tiao), ST36 (Zu San Li), GB34 (Yang Ling Quan), ST40 (Feng Long), ST41 (Jie Xi) and BL40 (Wei Zhong), the lesions, and trigger points. In addition, Chinese herbal medicine (Koda Pharmaceutical Co., Taiwan) including Zheng Gu Zi Jin Dan (正骨紫金丹 : 1 g), Shiuh Duann(續斷 : 0.2 g), Du Zhong(杜仲 : 0.2 g), Mo Yao(沒藥 : 0.2 g), Ru Xian(乳香 : 0.2 g) and Pyrite(自然銅 : 0.2 g) were orallly mdeicated BID for 0\9days in case 2. Walking was possible after session 11 for 4 weeks in case 1 and after session 6 for 2 weeks in case 2, respectively.

• Proceedings of the KIEE Conference
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• summer
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• pp.737-739
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• 2004

The domestic power system established with Cost-Based-Pricing(CBP) from April 2001. The system is a uniform pricing system. System Operator(50) establishes a Price Setting Schedule by the prediction of consumption and the presented bid price(generation cost) of the generation utility. But the Price Setting Schedule doesn't take account of the constraint of the system. This cause a transmission congestion, constrained-on generation and constrained-off generation. This Paper search the way of the increasing efficiency of domestic power system through the redemption of congestion charge.

• Proceedings of the KIEE Conference
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• 2001.11b
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• pp.170-172
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• 2001

This paper describes the settlement rules in the basic market design for Korean Wholesale Electricity Market. The settlement rules are to be exercised after the Bid and Offer Processes as described in the pool rules(within the market rules) and to determine the payments to Generators, charges to Energy Purchasers and charges or payments to the Transmission Asset Owner(TAO).

• YAKHAK HOEJI
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• v.45 no.6
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• pp.664-669
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• 2001

Acebutolol is almost absorbed after oral administration, but its bioavailability is reduced because of considerable first-pass metabolism through the gastrointestinal tract and liver. The purpose of this study was to report the pharmacokinetic changes of acebutolol (15 mg/kg,oral) and its main metabolite, diacetolol in rabbits pretreated (15 mg/kg, oral) and coadministered (15 mg/kg, S. C., bid for 3 days) with diltiazem. The plasma concentration and area under the plasma concentration-time curves (AUC) of acebutolol and diacetolol were significantly increased in rabbits pretreated and coadministered with diltazem. The elimination rate constant ( $K_{el}$ ) and total body clearances (CL $_{t}$) of acebutolol and diacetolol were significantly decreased and half-life of those were significantly prolonged in the rabbit. Metabolite percentage rate of diacetolol to the plasma concentration of total acebutolol in rabbits pretreated and coadministered with diltiazem were significantly decreased. The results suggest that the dosage of acebutolol should be adjusted when the drug would be administered chronically with diltiazem in a clinical situation.n.

• Journal of architectural history
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• v.19 no.6
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• pp.173-186
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• 2010

This article is about the study on construction contractors in Daegu during the Japanese Colonial Rule. The first construction contractors who involved with Kyong-bu railroad work in 1904 personally. But, after they go through the bidding method, changed by the Japanese Government General of Korea in 1922, and the second bid rigging in 1932, contractors was changed gradually the organization into unlimited partnership, limited partnership or stock corporation. The number of them was increased. Most contractors were Japanese, and organized a limited partnership which has been capitalized at 30,000 won. On the other hand, Korean contractors couldn't work at an important part, except for the personal activities of Youngsil Lee(李永實). They were usually employed as consultants or field deputies by Japanese contractors. After the Liberation, Japanese construction contractors returned to Japan, however Tatuoka-kumi(龍岡組), Yasiro-kumi(屋代組) and Sakano-kumi(坂野組) that was leading the business which were took over by Koreans. They were leading architecture field in Daegu.

• Journal of the Earthquake Engineering Society of Korea
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• v.20 no.1
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• pp.33-43
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• 2016

This study investigates the performance of hollow precast segmental bridge columns with reinforcement details for material quantity reduction. The proposed triangular reinforcement details are economically feasible and rational, and facilitate shorter construction periods. The precast segmental bridge columns provides an alternative to current cast-in-place systems. We tested a model of hollow precast segmental bridge columns under a constant axial load and a quasi-static, cyclically reversed horizontal load. We used a computer program, Reinforced Concrete Analysis in Higher Evaluation System Technology (RCAHEST), for analysis of reinforced concrete structures. The used numerical method gives a realistic prediction of performance throughout the loading cycles for hollow precast segmental bridge column specimens investigated. As a result, proposed reinforcement details for material quantity reduction was equal to existing reinforcement details in terms of required performance.

• The Transactions of the Korea Information Processing Society
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• v.6 no.2
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• pp.459-466
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• 1999

I broadband ISDN every packet will show different result if it would be processed according to its usage by the server. That is, normal data won't show big differences if they would be processed at normal speed. But it will improve the quality of service to process some kinds of data - for example real time video or voice type data or some data for a bid to by something through the internet - more fast than the normal type data. solution for this problem was suggested - priority packets. But the analyses of them are under way. Son in this paper a switching system for an output queuing model in a single server was assumed and some packets were given priorities and analysed. And correlation, simulating real life situation, was given too. These packets were analysed through three cases, first packets having no correlation, second packets having only correlation and finally packets having priority three cases, first packets having no correlation, second packets having only correlation and finally packets having priority and correlation. The result showed that correlation doesn't affect the mean delay time and the high priority packets have improved mean delay time regardless of the arrival rate. Those packets were assumed to be fixed-sized like ATM fixed-sized cell and the contention strategy was assumed to be short bus contention method for the output queue, and the mean delay length and the maximum 버퍼 length not to lose any packets were analysed.

• Journal of Korean Neurosurgical Society
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• v.60 no.4
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• pp.391-396
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• 2017

Objective : To investigate the neuroprotective effects of Ginkgolide B (GB) against ischemic stroke-induced injury in vivo and in vitro, and further explore the possible mechanisms concerned. Methods : Transient middle cerebral artery occlusion (tMCAO) mice and oxygen-glucose deprivation/reoxygenation (OGD/R)-treated N2a cells were used to explore the neuroprotective effects of GB. The expression of brain-derived neurotrophic factor (BDNF) was detected via Western blot and qRT-PCR. Results : GB treatment (4 mg/kg, i. p., bid) significantly reduced neurological deficits, water content, and cerebral infarct volume in tMCAO mice. GB also significantly increased Bcl-2/Bax ratio, reduced the expression of caspase-3, and protected against OGD/R-induced neuronal apoptosis. Meanwhile, GB caused the up-regulation of BDNF protein in vivo and in vitro. Conclusion : Our data suggest that GB might protect the brain against ischemic insult partly via modulating BDNF expression.