• Title/Summary/Keyword: tBUT

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Detection of Toxoplasma gondii in experimentally infected porcine blood and tissues by polymerase chain reaction (Polymerase chain reaction을 이용한 실험적 감염 돼지의 혈액과 조직으로부터 Toxoplasma gondii 검출)

  • Suh, Myung-deuk;Shin, Gee-wook
    • Korean Journal of Veterinary Research
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    • v.41 no.1
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    • pp.89-98
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    • 2001
  • This study was conducted to detect the toxoplasma specific-DNA in circulating blood and organs collected from slaughtered pigs at slaughtering house and experimentally infected pigs with Toxoplasma gondii tachyzoites by polymerase chain reaction(PCR), and also PCR was applied to diagnose for acute phase of swine toxoplasmosis as a newly developed diagnostic test. The sensitivity of oligonucleotide primer, T-1 & T-2, designed from toxoplasma B1 gene amplification method was compared with Tp parasite detection by mouse inoculation(MI). On the other hand, latex agglutination test(LAT) was conducted to detect the serum antibodies comparing with the detection of toxoplasma by PCR and MI. The results obtained were summarized as follows. PCR was able to determine at the lowest level of $10^0/ml$ T. gondii in blood samples which were blended with a serial diluted T gondii in vitro. On the other hand, $10^2/5g$ of T gondii could detect from a variety of tissues including lung, diaphragm, liver, heart, spleen and brain in vitro. The primer was proved to specifically determine T gondii in blood and tissues in vitro but it did not detect Neospora caninum used as a negative control. DNA of T. gondii was effectively extracted by freezing, thawing and grinding twice both tissues mixed with T gondii in vitro and in experimentally infected pig's tissues. PCR detected specific DNA in the blood of experimentally infected pigs at 108 hrs and 120 hrs post-infection, it was the same time that the pigs showed fever and parasitaemia. In case of tissue, specific DNA was, however, detected only lung from experimentally infected pigs. Even though the duration of acute phase was from 3 to 7 days post-infection, but the latex agglutination test (LAT) results appeared from 8 days post-infection. A comparison of sensitivity in determining T gondii in blood samples between PCR and MI, PCR positive rate ranged from 25 to 33.3%, but that of MI covered from 75 to 100%.

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생리활성 추출물의 첨가급여가 육계의 성장능력과 혈청 및 고기의 콜레스테롤 함량, 지질산화에 미치는 영향

  • Lee, U-Jin;Park, Jae-In;Kim, Chang-Hyeok;Lee, Gyu-Ho
    • Proceedings of the Korea Society of Poultry Science Conference
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    • 2005.11a
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    • pp.72-73
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    • 2005
  • This study was carried out to investigate the effects of extracts supplements of pine nut cone on the broiler performance and the cholesterol content, the lipid oxidation of serum and meat of broilers. The control group was fed a common basal diet without antibiotics and the treatment group were fed a common basal diet with extracts of pine nut cone of 500ppm(T1), illite 1% +extracts of pine nut cone of 500ppm(T2), pine nut cone powder of 2.5%(T3) for 5 weeks. The weight gain and the feed intake were significantly higher treatment than control. The cholesterol content of serum was significantly decreased in T1, T2 group. The breast was also significantly decreased in T1, T2 group. The thigh was not different among treatment, but the cholesterol content of serum was significantly lower in T1, T2 treatment than control. TBARS of the brest and the thigh showed significantly lower than control. POV of the brest and the thigh showed different among treatment, but there were no correlations among treatment.

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Age-related Changes in Luteinizing Hormone and Testosterone Levels in Korean Men (한국 남성의 혈중 Luteinizing Hormone과 Testosterone 수준의 연령-관련 변화)

  • Lee, Sung-Ho;Ahn, Ryun-Sup;Kwon, Hyuk-Bang
    • Development and Reproduction
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    • v.12 no.1
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    • pp.57-66
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    • 2008
  • Changes in luteinizing hormone (LH), serum testosterone (T), and salivary T levels with age were examined in Korean men. Serum was obtained from 167 Korean men of different ages ($20{\sim}69\;y$), and the serum LH and T levels were measured. Saliva samples were also obtained, and the salivary T level was determined. The LH levels did not change considerably until 40 y of age (20s, $2.5{\pm}1.0$; 30s, $2.7{\pm}1.5$; and 40s, $2.5{\pm}1.8\;mIU/mL$) but increased significantly around 50 y (50s, $3.7{\pm}1.8$ and 60s, $3.1{\pm}1.7\;mIU/mL$). Further, the serum T levels also did not change until 40 y of age (20s, $5.3{\pm}2.6$, 30s, $4.4{\pm}1.4$, 40s, $4.1{\pm}1.5\;ng/mL$) but decreased significantly at 50 y (50s, $3.4{\pm}1.5$; 60s, $2.6{\pm}0.8\;ng/mL$). The salivary T levels also showed small changes until the age of 40 y ($20s{\sim}40s$, $0.11{\pm}0.015\;ng/mL$) but decreased significantly at 50 y ($0.08{\pm}0.03\;ng/mL$). Thus, the relative ratio of salivary T to serum T levels did not change significantly in all the ages examined ($2.4{\pm}0.9%$). Linear regression analysis predicted that the LH levels increased 1.5%/y while the serum and salivary T levels decreased 1%/y and 0.8%/y, respectively. The serum T/LH ratio did not change considerably until the age of 40 y ($20s{\sim}40s$, $2.27{\pm}0.14$) but decreased significantly ($1.2{\pm}1.0$) at 50 y. Age-related changes in the salivary T/LH ratio were very similar to those in the serum T/LH ratio. These results demonstrated that LH and T levels in serum or saliva did not change considerably until 40 y of age; instead, in Korean men, from 50 y of age, the LH level increased, while the T level decreased. This suggests that primary testicular failure that occurred due to aging (approximately 50 y) and caused this phenomenon. The present study also shows that the salivary T level can be an indicator of the free T level in serum although the salivary T level correlates weakly with the total T level in serum (r=0.53). Thus, information regarding salivary T levels may be useful for studying the age-related changes occurring in male testicular physiology.

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Effects of Tween 80 on In Vitro Fermentation of Silages and Interactive Effects of Tween 80, Monensin and Exogenous Fibrolytic Enzymes on Growth Performance by Feedlot Cattle

  • Wang, Y.;McAllister, T.A.;Baah, J.;Wilde, R.;Beauchemin, K.A.;Rode, L.M.;Shelford, J.A.;Kamande, G.M.;Cheng, K.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.7
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    • pp.968-978
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    • 2003
  • The effects of monensin, Tween 80 and exogenous fibrolytic enzymes on ruminal fermentation and animal performance were studied in vitro and in vivo. In Expt 1, the effects of the surfactant Tween 80 (0.2% wt/wt, DM basis) on ruminal fermentation of alfalfa, corn and orchardgrass silages were investigated using in vitro gas production techniques. Tween 80 did not affect (p>0.05) cumulative gas production at 24 h, but it reduced (p<0.05) the lag in fermentation of all three silages. With corn silage and orchardgrass silage, gas production rates and concentrations of total volatile fatty acids (VFA) were increased (p<0.05) by Tween 80; with alfalfa silage, they were reduced (p<0.05). Tween 80 increased (p<0.05) the proportion of propionate in total VFA, and reduced (p<0.05) acetate to propionate ratios (A:P) with all three silages. In Expt 2, exogenous fibrolytic enzymes (E; at 0, 37.5 or 75 g/tonne DM), monensin (M; at 0 or 25 ppm and Tween 80 (T; at 0 or 2 L/tonne DM) were added alone or in combination to backgrounding and finishing diets fed to 320 crossbred steers in a feeding trial with a $3{\times}2{\times}$2 factorial arrangement of treatments. The backgrounding and finishing diets contained barley grain and barley silage in ratios of 57.8:42.2 and 93.5:6.5 (DM basis), respectively. Added alone, none of the additives affected DM intake (p>0.1) in the backgrounding or in the finishing period, but interactive $M{\times}T$ effects were observed in the finishing period (p=0.02) and overall (p=0.04). In the finishing period, T without M tended to reduce DM intake (p=0.11), but T with M increased (p=0.05) DM intake. Monensin increased average daily gain (ADG) during backgrounding (p=0.07) and finishing (p=0.01), and this ionophore also improved overall feed efficiency (p=0.02). Warm carcass weight was increased (p<0.001) by M, but dressing percentage was reduced (p=0.07). In the backgrounding period, T increased ADG by 7% (p=0.06). Enzymes increased (p=0.07) ADG by 5 and 6% (low and high application rates, respectively) during backgrounding, but did not affect (p>0.10) ADG during finishing, or overall feed efficiency. Whereas T enhanced the positive effects of M on ADG during backgrounding (p=0.04) and overall (p=0.05), it had no impact (p>0.1) on the effects of E. Interactions between M and T suggest that the surfactant may have potential for enhancing the positive effects of monensin on beef production, but this requires further research.

Efect of Mixed-Culture Soil on Growth Characteristics and Nutrient Silage Corn (혼파재배 토양이 옥수수 생육특성 및 영양수량에 미치는 영향)

  • 이상무;문상호;전병태
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.16 no.4
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    • pp.283-290
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    • 1996
  • This experiment was carried out to determine the effects of soil condition that previous crop cultivated after [TO: Soil of corn cultivated, T1 : Soil of rye monoculture cultivated(l50kg/ha), T2 : Soil of mixedculture cultivated(Rye : Red clover = 120 : 3koa). T3 : Soil of mixed-culture cultivated(Rye : Red clover = 90 : 6kg/ ha), T4 : Soil of mixedculture cultivated(Rye : Red clover = 60 : 9kg/ha), T5 : Soil of mixedculture cultivated (Rye: Red clover = 30 : 12kg/ha), T6 : Soil of red clover monoculture cultivated(l5kgha)) on the growth characteristics and nutrient yield of silage corn. The results are summarized as follows. 1. The plant length of silage corn was high in order of T6>T5>T4 treatment. In the leaf length, T5 and T6 treatment showed short compared to the other treatments, but they were wider than the other treatments in leaf width. In the number of leaf, T6 treatment was the highest as 14 leaves per plant, while T1 treatment showed lower than the other treatments as 12.6 leaves per plant. 2. In the stem hardness, ear length, ear width and ear full degree, soil treatment of red clover mono-culture cultivation(T6) showed higher than the other treatment as 5.1 1kg/$cm^2$, 20.8~~1, 7.7cm and 97%. respectively (P<0.01, 0.05). Ihe leaf of summer depression did not many occurrence, but T6 treatment was lower than the other treatment as 0.8 leaf per plant. 3. ADF and NDF composition were not affected by soil condition. Crude protein composition of T6 treatment was the highest as 7.8%. while TI treatment was the lowest as 6.9%. Fresh weight yield(59,083kg/ha), dry matter yield(21,743kg/ha), crude protein yield(l,369kg/ha) and TDN yield(15,800kg/ha) at T6 treatment were much more increased by 41.9, 47.5, 57.4 and 49% than TI treatment(39,410, 14,259, 827 and 10,056kg/ha).

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Gab Theory and Minimal Intuition on Truth (간극이론과 진리에 대한 최소직관)

  • Lee, Jinhee
    • Korean Journal of Logic
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    • v.19 no.2
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    • pp.145-184
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    • 2016
  • Williamson(1994) proved incompatibility of Gab Theory and Tarski T-schema. But this does not means that Gab Theory could not involve intuition on truth that is expressed by T-schema. I will show that Gab Theory and mutual entailment of 'p' and 'it is true that p'(p⊨T

    and T

    ⊨p) are compatible. It will draw that Gab Theory can involve minimal intuition on truth. After all what I want to reveal is logical space for Gab Theory through the compatibility of the mutual entailment and negation of the Principle of Bivalence. To prove the compatibility, I will present a consequent relation which should be accepted whenever we accept Gab Theory and demonstrate Gab Theory and the mutual entailment imply following two thesis; 1) not-T

    and T are not equivalent. 2) p entails T

    but not-T

    does not entails not-p.

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Effect of Feeding Dietary Oils on Physico-Chemical Changes of Pork during Storage (오일 급여가 돈육의 저장 중 물리ㆍ화학적 변화에 미치는 영향)

  • 진상근;김일석;송영민;정기화;이성대;김희윤;남기윤;하경희;강양수
    • Food Science of Animal Resources
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    • v.23 no.3
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    • pp.227-235
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    • 2003
  • The results were obtained from pigs which had been fed finishing pig diets containing 5% beef tallow(C) as control and 2% perilla seed oil(Tl), 3% beef tallow and 2% squid viscera oil(T2), 3% beef tallow and 2% CLA(conjugated linoleic acid, T3). All porks were stored at 1$^{\circ}C$ for 28 days. pH value of control group was higher than other treatments. Water holding capacity(WHC) did not show any significant difference among treatments, however, WHC of C and T3 was increased as storage days increased. Protein solubility of T3 was higher than the other treatments, but that of all groups increased up to 14 days of storage and then decreased. The a* value of C was higher than the others, but b* value was low on 28 days of storage. Volatile base nitrogen(VBN) value of T3 showed the highest level, but that of Tl was the lowest. Thiobarbituric acid reactive substances(TBARS) of T2 and T3 were' higher than those of C and Tl. In sensory analysis, meat color and overall acceptability of C were higher than those of the other treatments in raw meat, and meat appearance was higher than level in Tl.

Effects of Seeding Dates and Growth Periods on the Growth Characteristics, Dry Matter Yield and Feed Value of Corn for Silage in Paddy Field (논토양에서 파종시기와 재배기간이 사일리지용 옥수수의 생육특성, 건물수량 및 사료적 가치에 미치는 영향)

  • Lee, Sang-Moo;Lee, Jae-Hun
    • Journal of Animal Science and Technology
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    • v.52 no.5
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    • pp.441-448
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    • 2010
  • This study was carried out to investigate the effects of the seeding dates and growth periods on the growth characteristics, dry matter yield and feed value of corn for silage in paddy field. The experimental design was arranged in a randomized block design with three replications. Treatments consisted of five seeding dates, 1 May (T1), 8 May (T2), 15 May (T3), 22 May (T4) and 29 May (T5). And harvesting time homologized by August 24. Therefore, growing periods were 115 days (T1), 108 days (T2), 101 days (T3), 94 days (T4) and 87 days (T5), respectively. In maturities at harvest time, T1, T2, T3, T4 and T5 were full dent, early dent, late dough, dough and early dough stage, respectively. Plant height and numbers of root system were highest in T5 (p<0.01), but leaf length was higher in T4 than others (p<0.05). Dead leaf, tip filling degree and ear circle were higher in T1 than others (p<0.05, 0.01). Leaf width, ear height, leaf number, ear length, stem diameter, stem hardness and brix were not significantly different among the treatments. Dry matter yield and TDN yield were higher in the order of T1 > T2 > T3 > T4 > T5 (p<0.01). Crude protein, crude ash, NDF, ADF and crude fiber were significantly higher in T4 (p<0.05, 0.01). But crude fat was the highest in T1, T3 was the lowest as compared to other treatments (p<0.01). Total EAA(essential amino acids) were higher in order of T4 > T3 > T1 > T2 > T5, and total NEAA (nonessential amino acids) were higher in order of T1 > T4 > T2 > T3 > T5, and total amino acids were higher in order of T4 > T1 > T3 > T2 > T5. But no significant differences were found among the treatments. TUFA (total unsaturated fatty acid) and TSFA (total saturated fatty acid) were in order of T1 > T2 > T3 > T5 > T4 (p<0.01). Minerals were the highest in T4 (4,721.25 mg/kg), and T2 (2,970.80 mg/kg) was the lowest as compared to other treatments (p<0.05). Based on the above results, seeding dates could be recommended as early May, and harvest times is yellow ripe stage for qualitative and quantitative production of corn for silage in rice paddy field soil.

ON A MOVING GRID NUMBERICAL SCHEME FOR HAMILTON-JACOBI EQUATIONS

  • Hong, Bum-Il
    • Journal of the Korean Mathematical Society
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    • v.33 no.2
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    • pp.249-258
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    • 1996
  • Analysis by the method of characteristics shows that if f and $u_0$ are smooth and $u_0$ has compact support, then the Hamilton-Jacobi equation $$ (H-J) ^{u_t + f(u_x) = 0, x \in R, t > 0, } _{u(x, 0) = u_0(x), x \in R, } $$ has a unique $C^1$ solution u on some maximal time interval $0 \leq t < T$ for which $lim_{t \to T}u(x, t) exists uniformly; but this limiting function is not continuously differentiable.

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The Counting Process of Which the Intensity Function Depends on States

  • Park, Jeong-Hyun
    • Communications for Statistical Applications and Methods
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    • v.4 no.1
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    • pp.281-292
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    • 1997
  • In this paper we are concered with the counting processes with intersity function $g_n(t)$, where $g_n(t)$ not only depends on t but n. It is shown that under certain conditions the number of events in [0, t] follows a generalizes Poisson distribution. A counting process is also provided such that $g_i(t)$$\neq$$g_i(t)$ for i$\neq$j and the number of events in [0, t] has a transformed geometric distribution.

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