• 제목/요약/키워드: t,t-MA

검색결과 629건 처리시간 0.033초

벤젠 대사산물에 의해 유도된 HL-60 세포의 8번 및 21번 염색체의 이수성 및 상호전좌 (Detection of Benzene Metabolite Induced Aneuploidy and Translocation in HL-60 Cells by Fluorescence in situ Hybridization using Whole Chromosome-specific Probes for Chromosome 8 and 21)

  • 김수영;정해원
    • 한국환경성돌연변이발암원학회지
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    • 제22권2호
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    • pp.90-96
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    • 2002
  • Benzene is a widespread human carcinogen, inducing leukemia and hematotoxicity. Exposure to benzene metabolites has been shown to cause genetic damage, including aneusomy and chromosome aberrations. Fluorescence in situ hybridization(FISH) procedure was used to determine if the benzene metabolite, 1, 2, 4-benzenetriol(BT), hydroquinone(HQ) and trans, trans-muconic acid(t,t-MA) induced specific chromosomal change in HL-60 cells. Treatment with BT, HQ and t,t-MA resulted in the induction of monosomy 8 and 21 in HL-60 cells in a dose-dependent manner. All of these metabolites also induced trisomy 8 and 21, but no correlation between frequencies of trisomy and concentration was found. Translocations between chromosome 8 and another unidentified chromosome [t(8:\ulcorner)], and between chromosome 21 and another unidentified chromosome [t(8:21)] were found. However, translocation between chromosome 8 and 21 [t(8:21)] was not found. Results indicate that the benzene metabolites, BT, HQ and t,t-MA, induce chromosome specific numerical and structural aberrations, and the fluorescence in situ hybridization (FISH) approach may be a useful and powerful technique for detection of aneuploidy.

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유증기 회수설비 유무에 따른 주유소 근로자들의 요중 trans, trans-Muconic acid, Hippuric acid에 관한 연구 (A Study on Urinary Trans, Trans-Muconic acid, Hippuric acid of gas station worker according to the use of gasoline vapor recovery system)

  • 최재준;원종욱;김치년;노재훈
    • 한국산업보건학회지
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    • 제24권2호
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    • pp.152-159
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    • 2014
  • Objectives: This study aims to investigate the excretion aspect of urinary t, t-MA and hippuric acid by measuring concentrations of urinary metabolites according to the use of gasoline vapor recovery system. Materials:In order to analyze urinary metabolites, samples from the 23 gas station workers ten gas stations in the Seoul and Gyeonggi Province area were collected once daily after work. In addition, a survey was conducted on work factors and lifestyle habits as factors affecting the concentration of urinary metabolites. Results: The average concentrations of t, t-MA and hippuric acid after work were $0.124{\pm}0.177mg/g$ creatinine and $0.557{\pm}0.251g/g$ creatinine among workers at gas stations where gasoline vapor recovery systems were installed. The average concentrations of t, t-MA and hippuric acid were $0.160{\pm}0.113mg/g$ creatinine and $0.682{\pm}0.619g/g$ creatinine among workes at gas stations where gasoline vapor recovery systems were not installed. Average concentrations were higher at gas stations where a gasoline vapor recovery system was not installed, but the differences were not statistically significant differences. Urinary t, t-MA and hippuric acid average concentrations of smokers and non-smokers were higher in the gas stations where gasoline a vapor recovery system was not installed. T, t-MA as a factor evaluation affecting the concentration of urinary metabolites was not statistically significant in all factors, while hippuric acid was statistically significant only for age(p=0.024). Conclusions: The average concentrations of urinary t, t-MA and hippuric acid were higher in gas stations where gasoline vapor recovery systems were not installed compared to gas stations where such a system was installed. There needs to be an assessment of biological monitoring according to refueling activity considering skin absorption of benzene and toluene and presence of gasoline vapor recovery system.

유산균을 첨가한 발효육의 이화학적 특성 및 산화억제 효과 (Physico-chemical Characteristics and Antioxidative Effect of Fermented Meat by Addition of Lactobacillus casei)

  • 한승관;홍용
    • 육가공
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    • 통권34호겨울호
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    • pp.40-47
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    • 2007
  • 본 연구는 돈육 등심 부위 근육을 이용하여 유산균을 첨가하지 않은 대조구와 10% 유산균 L. casei KCTC 3109를 첨가한 처리구 T1 구의 비교실험을 통하여 이화학적 특성 및 항산화 효과를 측정하였다. 돈육의 일반성분에서 수분, 지방, 회분은 유의적인 차이는 없었으나 단백질 함량은 대조구가 T1구보다 유의적으로 높았다. 등심 부위의 pH와 보수성은 T1구가 유의적으로 높았으나(p<0.05) 가열감량은 대조구가 높은 경향을 보였다. 육색은 명도, 적색도 및 황색도에서 대조구가 T1구보다 유의적으로 높게 나타났다(p<0.05). TBARS치는 3일 간 정 치 배 양 한 T1구 가 0.02 MA mg/1,000 g으로 대조구 0.19MA mg/1,000 g보다 유의적으로 낮았다. (p<0.05).

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유산균을 첨가한 발효육의 이화학적 특성 및 산화억제 효과 (Physico-Chemical Characteristics and Antioxidative Effect of Fermented Meat by Addition of Lactobacillus casei)

  • 한승관;홍용
    • 한국축산식품학회지
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    • 제26권2호
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    • pp.218-222
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    • 2006
  • 본 연구는 돈육 등심 부위 근육을 이용하여 유산균을 첨가하지 않은 대조구와 10% 유산균 L. casei KCTC 3109를 첨가한 처리구 $T_1$구의 비교실험을 통하여 이화학적 특성 및 항산화 효과를 측정하였다. 돈육의 일반성분에서 수분, 제방, 회분은 유의적인 차이는 없었으나 단백질 함량은 대조구가 $T_1$구보다 유의적으로 높았다. 등심 부위의 pH와 보수성은 $T_1$구가 유의적으로 높았으나(p<0.05) 가열감량은 대조구가 높은 경향을 보였다. 육색은 명도, 적색도 및 황색도에서 대조구가 $T_1$구보다 유의적으로 높게 나타났다(p<0.05). TBARS 치는 3일간 정치배양한 $T_1$구가 0.02 MA mg/1,000 g으로 대조구 0.19 MA mg/1,000 g보다 유의적으로 낮았다(p<0.05).

소백산 육괴 서남부인 승주-순천 일대의 화강암질 편마암과 반상변정질 편마암의 변성진화과정 (Metamorphic evolution of granitic and porphyroblastic gneisses in the Seungju-Suncheon area, the southwestern part of the Sobacksan Massif)

  • 오창환;전은영;박배영;안건상;이정후
    • 암석학회지
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    • 제9권3호
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    • pp.121-141
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    • 2000
  • 소백산육피의 서남부인 승주-순천 일대에는 화강암질 편마암과 반상변정질 편마암이 광범위하게 분포되어 있다. 화강암질 편마암으로부터 두 그룹의 변성 온도-압력 조건이 추정된다. 석류석 내부 그리고 후퇴변성작용을 약하게 받은 시료로부터 $622-760^{\circ}C/6.2~7.4\;kbar$의 Group I과 후퇴변성에 의하여 파이로프가 감소하고 스페살틴이 증가한 석류석 주변부로부터 $606~785^{\circ}C/3.7~5.4\;kbar$의 Group II가 추정된다. 반상변정질 편마암으로부터 추정된 온도-압력 조건은$ 489~669^{\circ}C/2.1~4.8\;kbar$로서 화강암질 편마암에서 얻어진 Group II의 온도-압력 조건과 유사하다. 화강암질 편마암과 반상변정질 편마암의 전암-석류석 Sm/Nd 동위원소 연대는 각각 $1417{\pm}52\;Ma과\;1421{\pm}14\;Ma$로 서로 일치한다. 이러한 자료는 화강암질 편마암이 경험한 Group I에 해당하는 중압형 변성작용이 화강암질 편마암 관입 이후 그리고 반상변정질 편마암의 관입 이전, 즉 1890 Ma와 2120 Ma 사이에 일어났을 가능성이 높으며, 두 편마암은 반상변정질 편마암 관입 이후 함게 1417~1421 Ma 시기에 저압형 변성작용을 받았음을 지시한다.

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Physico-chemical attributes, sensory evaluation and oxidative stability of leg meat from broilers supplemented with plant extracts

  • Yang, Eun Ju;Seo, Ye Seul;Dilawar, Muhammad Ammar;Mun, Hong Seok;Park, Hyeoung Seog;Yang, Chul Ju
    • Journal of Animal Science and Technology
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    • 제62권5호
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    • pp.730-740
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    • 2020
  • This feeding trial was conducted to investigate the effects of Mentha arvensis (MA) and Geranium thunbergii (GT) in drinking water on physicochemical attributes, sensory qualities, proximate analysis and oxidative stability of broiler leg meat. One hundred and twenty broiler chicks were assigned to 1 of 4 dietary treatments for 5 weeks. The dietary treatments were 1) control, 2) T1 (0.1% 1 MA:1 GT), 3) T2 (0.1% 1 MA:4 GT), 4) T3 (0.1% 4 MA: 1 GT). The water holding capacity and cooking loss were improved (p < 0.05) in T2 and T3. The flavor, texture and acceptability of leg meat by consumers were significantly increased in T2 relative to the control (p < 0.05). The crude protein content was increased in T3 while the crude fat decreased in T2 (p < 0.05). Moreover, broilers supplemented with plant extracts had the lowest leg meat TBARS (thiobarbituric acid reactive substances) values after 2 weeks of storage as compared with the control. Total phenolic contents and 1-1-diphenyl 2 picrylhydrazyl (DPPH) activity were also better in the T2 group (p < 0.05) compared with the control, whereas 2,2-Azinobis-3 ethytlbenzothiazoline-6-sulfonic acid (ABTS+) remained unaffected. Overall, these results demonstrate that broiler drinking water with the inclusion of plant extract combination can be used to enhance the oxidative stability, shelf life and quality characteristics of broiler leg meat without compromising the growth performance.

각섬암상 변성작용 중 갈렴석과 저어콘의 평형 성장 (Equilibrium Growth of Allanite and Zircon during Amphibolite-facies Metamorphism)

  • 김윤섭
    • 암석학회지
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    • 제27권2호
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    • pp.97-104
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    • 2018
  • 한반도 중서부 서해 연안 대이작도에 산출하는 토날라이트질 편마암 시료(DE43)의 저어콘 결정에서 U-Pb 동위원소 및 희토류 원소 성분을 이차이온질량분석기를 이용하여 측정하였다. 최대 ${\sim}300{\mu}m$ 크기의 저어콘 결정들은 드물게 과성장 띠를 가진다. 고원생대 연령을 가지는 중심부와 달리 저어콘의 과성장 띠로부터 분석한 두 개의 점 분석에서 $256{\pm}23Ma(1{\sigma})$, and $221{\pm}7Ma(1{\sigma})$$^{206}Pb/^{238}U$ 연령을 구하였다. 이 과성장 띠들은 낮은 Th/U 비(<0.01)와 경희토류 원소가 강하게 결핍된 지화학적 특징을 보인다. 이 저어콘의 페름기-트라이아스기 겉보기 연령은 동일한 시료에서 분석한 갈렴석 $^{208}Pb/^{232}Th$ 연령($227{\pm}7Ma(t{\sigma})$)과 오차범위 내에서 일치하며, 갈렴석과 저어콘이 ~227 Ma때 평형 성장하였음을 지시한다. 한편, 동일한 시료에서 측정된 보다 더 젊은 갈렴석 $^{208}Pb/^{232}Th$ 연령과 $^{206}Pb/^{238}U$ 연령(각각 $213{\pm}4Ma(t{\sigma})$$186{\pm}9Ma(t{\sigma})$)은 주변에 산출하는 후기 트라이아스기 및 쥐라기 화강암의 관입에 수반된 알칼리성 유체 유입에 의한 Pb 손실에 기인한 것으로 보인다.

타액선 신티그라피를 이용한 정상 타액선기능의 정량분석 (Quantitative Analysis of Normal Salivary Gland Function using Salivary Scintigraphy)

  • 김희진;고명연;박준상
    • Journal of Oral Medicine and Pain
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    • 제25권4호
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    • pp.345-353
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    • 2000
  • This study was to investigate normal salivary flow rates and normal indices of Quantitative analysis of salivary scintigraphy. 96 adult volunteers were studied by Questionnaire evaluating salivary conditions and clinical examinations. 35(male 23, female 12, age range 23-31years) that absented subjective and objective symptoms related saliva were classified as normal group. The normal group underwent measurement unstimulated and stimulated salivary flow rates and salivary scintigraphy. The obtained results were as follows: 1. There were not significant in sex differences of unstimulated and stimulated salivary flow rates. The unstimulated salivary flow rate was $0.66{\pm}0.41g/min$, stimulated salivary flow rates was $1.61{\pm}0.69g/min$. 2. As comparing of parameters of salivary scintigraphy, the Uptake ratio(UR), $T_{max}$, $T_{min}$, Maximum accumulation (MA), Maximum secretion(MS) of parotid and submandibular glands were not significant in sex and side-ralated differences. 3. The UR, $T_{max}$, MA, MS of parotid gland were significantly higher than those of submandibular gland; in the parotid gland, UR, $3.67{\pm}0.88$, $T_{max}$, $18.77{\pm}0.43min$, MA, $41.35{\pm}9.22%$, MS, $43.13{\pm}9.13%$; in the submandibular gland, UR, $3.04{\pm}0.10$, $T_{max}$, $18.48{\pm}0.52min$, MA, $36.47{\pm}14.18%$, MS, $36.88{\pm}12.20%$. 4. As classifying of time-activity curve, the most of parotid gland was N-type(97.1%), submandibular gland was observed in order of M-type(67.1%), N-type(21.4%), F-type(11.4%), however, was not observed S-type. 5. As the type of time-activity curve of submandibular gland was more flattened, the UR, $T_{max}$, MA, MS were significantly decresed.

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최대 인접 병합 방법을 적용한 방향 그래프의 병목지점 탐색 알고리즘 (A Bottleneck Search Algorithm for Digraph Using Maximum Adjacency Merging Method)

  • 이상운
    • 한국인터넷방송통신학회논문지
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    • 제12권5호
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    • pp.129-139
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    • 2012
  • 공급처 s와 수요처 t, 호가 수용량을 갖고 있는 방향 그래프 망 $D=(N,A),n{\in}N,a=c(u,v){\in}A$에 대해, 공급처 s에서 수요처 t로의 최대 흐름양은 N을 $s{\in}S$$t{\in}T$의 집합으로 분리시키는 최소절단값이 결정한다. 최소절단을 찾는 대표적인 알고리즘으로는 수행복잡도 $O(NA^2)$의 Ford-Fulkerson이 있다. 이 알고리즘은 가능한 모든 증대경로를 탐색하여 병목지점을 결정한다. 알고리즘이 종료되면 병목지점들의 조합으로 N=S+T의 절단이 되는 최소 절단을 결정해야 한다. 본 논문은 S={s}, T={t}를 초기값으로 설정하고, 망의 최대 수용량 호 $_{max}c(u,v)$를 인접한 S나 T로 병합시키고 절단값을 구하는 최대인접병합 알고리즘을 제안하였다. 최대인접병합 알고리즘은 n-1회를 수행하지만 알고리즘 수행 과정에서 최소절단을 찾는 장점을 갖고 있다. Ford-Fulkerson과 최대인접병합 알고리즘을 다양한 8개의 방향 그래프에 적용한 결과 제안된 알고리즘은 수행복잡도 O(N)인 n-1회 수행 과정에서 최소절단을 쉽게 찾을 수 있었다.

울릉분지 돌고래 시추공의 생층서: 고환경 및 이들의 석유탐사에의 응용

  • 이성숙;윤혜수;배부영;박세진;이의형;강소라;김재호;김기현
    • 한국석유지질학회:학술대회논문집
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    • 한국석유지질학회 1999년도 제6차 학술발표회 발표논문집
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    • pp.50-67
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    • 1999
  • Biostratigraphic and paleoenvironmental analyses are carried out on cutting samples from the Dolgorae wells drilled in the Ulleung basin. The clayey, silty, and sandy sediments of the wells yield various microfossil assemblages of relatively good preservation, among which five fossil groups are reported; a total of 97 foraminiferal species of 66 genera, 19 nannofossil species of 12 genera, 86 ostracod species of 41 genera, 107 diatom species of 44 genera, and 124 dinoflagellate cysts species of 45 genera. Based on microfossils the geologic ages of the Dolgorae wells are dated to be from late Early Miocene to Early Pleistocene. Several biohorizons are defined in Neogene successions by the LOD (Last Ocurrence Datum) and FOD (First Ocurrence Datum) of marker species including G. truncatulinoides (LOD: 1.9 Ma) of foraminifera; C. macintyeri (LOD: 1.64-1.60), G. oceanica (FOD: 1.65 Ma), G. caribbeanica (1.72 Ma), D. brouweri (LOD: 2 Ma), R. pseudoumbilica (LOD: 3.66 Ma), P. lacunosa (FOD: 4.2 Ma) of nannofossils; S. ellipsoideus (LOD: 4 Ma), S. palcacantha (LOD: 10.2), C. giusepei (LOD: 14 Ma) of dinocysts; D. seminae v. fossilis (FOD: 3.7 Ma), T. antiqua (LOD: 1.7 Ma), T. convexa (LOD: 2.4 Ma), N. kamtschatica (LOD: 2.58 Ma), T. oestrupii(FOD: 5.1 Ma) of diatoms. Abundance patterns of microfossils throughout the wells reflect changes in paleoenvironmental and sedimentological settings of the basin in relation to sea-level variations. According to these data the large-cycle and small-cycle changes of transgression and regression phases are observed in terrestrial to marine sediments. This high-resolution sequence biostratigraphy established by various fossil groups enabled more reliable correlation between strata and refined interpretation on deposition systems of the basin. It also proved to provide fundamental and precise informations regarding stratigraphic correlation, tectonic events, basin, and depositional history for hydrocarbon explorations, especially in collaboration with seismic-stratigrahic analyses.

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