• Title/Summary/Keyword: t,t-MA

Search Result 629, Processing Time 0.034 seconds

Detection of Benzene Metabolite Induced Aneuploidy and Translocation in HL-60 Cells by Fluorescence in situ Hybridization using Whole Chromosome-specific Probes for Chromosome 8 and 21 (벤젠 대사산물에 의해 유도된 HL-60 세포의 8번 및 21번 염색체의 이수성 및 상호전좌)

  • 김수영;정해원
    • Environmental Mutagens and Carcinogens
    • /
    • v.22 no.2
    • /
    • pp.90-96
    • /
    • 2002
  • Benzene is a widespread human carcinogen, inducing leukemia and hematotoxicity. Exposure to benzene metabolites has been shown to cause genetic damage, including aneusomy and chromosome aberrations. Fluorescence in situ hybridization(FISH) procedure was used to determine if the benzene metabolite, 1, 2, 4-benzenetriol(BT), hydroquinone(HQ) and trans, trans-muconic acid(t,t-MA) induced specific chromosomal change in HL-60 cells. Treatment with BT, HQ and t,t-MA resulted in the induction of monosomy 8 and 21 in HL-60 cells in a dose-dependent manner. All of these metabolites also induced trisomy 8 and 21, but no correlation between frequencies of trisomy and concentration was found. Translocations between chromosome 8 and another unidentified chromosome [t(8:\ulcorner)], and between chromosome 21 and another unidentified chromosome [t(8:21)] were found. However, translocation between chromosome 8 and 21 [t(8:21)] was not found. Results indicate that the benzene metabolites, BT, HQ and t,t-MA, induce chromosome specific numerical and structural aberrations, and the fluorescence in situ hybridization (FISH) approach may be a useful and powerful technique for detection of aneuploidy.

  • PDF

A Study on Urinary Trans, Trans-Muconic acid, Hippuric acid of gas station worker according to the use of gasoline vapor recovery system (유증기 회수설비 유무에 따른 주유소 근로자들의 요중 trans, trans-Muconic acid, Hippuric acid에 관한 연구)

  • Choi, Jaejun;Won, Jong-Uk;Kim, Chi-Nyon;Roh, Jaehoon
    • Journal of Korean Society of Occupational and Environmental Hygiene
    • /
    • v.24 no.2
    • /
    • pp.152-159
    • /
    • 2014
  • Objectives: This study aims to investigate the excretion aspect of urinary t, t-MA and hippuric acid by measuring concentrations of urinary metabolites according to the use of gasoline vapor recovery system. Materials:In order to analyze urinary metabolites, samples from the 23 gas station workers ten gas stations in the Seoul and Gyeonggi Province area were collected once daily after work. In addition, a survey was conducted on work factors and lifestyle habits as factors affecting the concentration of urinary metabolites. Results: The average concentrations of t, t-MA and hippuric acid after work were $0.124{\pm}0.177mg/g$ creatinine and $0.557{\pm}0.251g/g$ creatinine among workers at gas stations where gasoline vapor recovery systems were installed. The average concentrations of t, t-MA and hippuric acid were $0.160{\pm}0.113mg/g$ creatinine and $0.682{\pm}0.619g/g$ creatinine among workes at gas stations where gasoline vapor recovery systems were not installed. Average concentrations were higher at gas stations where a gasoline vapor recovery system was not installed, but the differences were not statistically significant differences. Urinary t, t-MA and hippuric acid average concentrations of smokers and non-smokers were higher in the gas stations where gasoline a vapor recovery system was not installed. T, t-MA as a factor evaluation affecting the concentration of urinary metabolites was not statistically significant in all factors, while hippuric acid was statistically significant only for age(p=0.024). Conclusions: The average concentrations of urinary t, t-MA and hippuric acid were higher in gas stations where gasoline vapor recovery systems were not installed compared to gas stations where such a system was installed. There needs to be an assessment of biological monitoring according to refueling activity considering skin absorption of benzene and toluene and presence of gasoline vapor recovery system.

Physico-chemical Characteristics and Antioxidative Effect of Fermented Meat by Addition of Lactobacillus casei (유산균을 첨가한 발효육의 이화학적 특성 및 산화억제 효과)

  • Han, Seung-Gwan;Hong, Yong
    • the MEAT Journal
    • /
    • s.34 winter
    • /
    • pp.40-47
    • /
    • 2007
  • This study was conducted to determine the effect of L. casei KCTC 3109 on physico-chemical characteristics and TBARS values of fermented pork meat. Each pork meat were allotted to two treatments ; Control (0%), T1 (supplemented with L. casei KCTC 3109 10%). The pH tenderness and water holding capacity (WHC) of T1 were higher than those of control (p<0.05), cooking loss of control was higher than T1. Water content, crude fat and ash were not significantly different, crude protein was higher in T1 compared with control (p<0.05). L*, a* and b* values of control were higher than those of T1 (p<0.05). TBARS values was higher in T1 (0.02 MA mg/1,000 g) than control (0.19 MA mg/1,000 g) (p<0.05).

  • PDF

Physico-Chemical Characteristics and Antioxidative Effect of Fermented Meat by Addition of Lactobacillus casei (유산균을 첨가한 발효육의 이화학적 특성 및 산화억제 효과)

  • Han Seung-Kwan;Hong Young
    • Food Science of Animal Resources
    • /
    • v.26 no.2
    • /
    • pp.218-222
    • /
    • 2006
  • This study was conducted to determine the effect of L. casei KCTC 3109 on physico-chemical characteristics and TBARS values of fermented pork meat. Each pork meat were allotted to two treatments ; Control (0%), $T_1$ (supplemented with L. casei KCTC 3109 10%). The pH tenderness and water holding capacity (WHC) of $T_1$ were higher than those of control (p<0.05), cooking loss of control was higher than $T_1$. Water content, crude Int and ash were not significantly different crude protein was higher in $T_1$ compared with control (p<0.05). $L^*,\;a^*$ and $b^*$ values of control were higher than those of $T_1$ (p<0.05). TBARS values was higher in $T_1$(0.02 MA mg/1,000 g) than control (0.19 MA mg/1,000g) (P<0.05).

Metamorphic evolution of granitic and porphyroblastic gneisses in the Seungju-Suncheon area, the southwestern part of the Sobacksan Massif (소백산 육괴 서남부인 승주-순천 일대의 화강암질 편마암과 반상변정질 편마암의 변성진화과정)

  • 오창환;전은영;박배영;안건상;이정후
    • The Journal of the Petrological Society of Korea
    • /
    • v.9 no.3
    • /
    • pp.121-141
    • /
    • 2000
  • Granitic and pophyroblastic gneisses are widely distributed in the Seungju-Suncheon area, the southwestern part of the Sobacksan Massif. Two groups of metamorphic P-T conditions are recognized from granitic gneiss. $622-760^{\circ}C/6.2~7.4\;kbar$(Group I) are estimated from garnet cores and samples with weak retrograde metamorphism. $606~785^{\circ}C/3.7~5.4\;kbar$(Group II) are estimated from garnet rims which have lower pyrope and higher spessartine contents due to the effect of retrograde metamorphism. The metamorphic P-T conditions estimated from porphyroblastic gneiss are $489~669^{\circ}C$, 2.1~4.8 kbar which are similar to the P-T conditions of Group II in the granitic gneiss. The whole rock-garnet Sm/Nd isotopic ages determined from granitic and porphyroblastic gneisses are, respectively, $1417{\pm}52\;Ma\;and\;1421{\pm}14\;Ma$. These date indicate that intermediate-P/T type metamorphism represented by Group I may have occurred between the intrusion of granite gneiss and the intrusion of porphyroblastic gneiss(between 1890 Ma~2120 Ma) and two gneisses experienced low-P/T type metamorphism after the intrusion of porphyroblastic gneiss at 1417~1421 Ma.

  • PDF

Physico-chemical attributes, sensory evaluation and oxidative stability of leg meat from broilers supplemented with plant extracts

  • Yang, Eun Ju;Seo, Ye Seul;Dilawar, Muhammad Ammar;Mun, Hong Seok;Park, Hyeoung Seog;Yang, Chul Ju
    • Journal of Animal Science and Technology
    • /
    • v.62 no.5
    • /
    • pp.730-740
    • /
    • 2020
  • This feeding trial was conducted to investigate the effects of Mentha arvensis (MA) and Geranium thunbergii (GT) in drinking water on physicochemical attributes, sensory qualities, proximate analysis and oxidative stability of broiler leg meat. One hundred and twenty broiler chicks were assigned to 1 of 4 dietary treatments for 5 weeks. The dietary treatments were 1) control, 2) T1 (0.1% 1 MA:1 GT), 3) T2 (0.1% 1 MA:4 GT), 4) T3 (0.1% 4 MA: 1 GT). The water holding capacity and cooking loss were improved (p < 0.05) in T2 and T3. The flavor, texture and acceptability of leg meat by consumers were significantly increased in T2 relative to the control (p < 0.05). The crude protein content was increased in T3 while the crude fat decreased in T2 (p < 0.05). Moreover, broilers supplemented with plant extracts had the lowest leg meat TBARS (thiobarbituric acid reactive substances) values after 2 weeks of storage as compared with the control. Total phenolic contents and 1-1-diphenyl 2 picrylhydrazyl (DPPH) activity were also better in the T2 group (p < 0.05) compared with the control, whereas 2,2-Azinobis-3 ethytlbenzothiazoline-6-sulfonic acid (ABTS+) remained unaffected. Overall, these results demonstrate that broiler drinking water with the inclusion of plant extract combination can be used to enhance the oxidative stability, shelf life and quality characteristics of broiler leg meat without compromising the growth performance.

Equilibrium Growth of Allanite and Zircon during Amphibolite-facies Metamorphism (각섬암상 변성작용 중 갈렴석과 저어콘의 평형 성장)

  • Kim, Yoonsup
    • The Journal of the Petrological Society of Korea
    • /
    • v.27 no.2
    • /
    • pp.97-104
    • /
    • 2018
  • The U-Pb isotopic and rare earth element compositions of zircon were measured using a SHRIMP from a tonalitic gneiss sample DE43 in Daeijak Island, central Korea. Zircon crystals, up to ${\sim}300{\mu}m$ in diameter, rarely contain thin overgrowth rims. In contrast to Paleoproterozoic cores, the $^{206}Pb/^{238}U$ ages of $256{\pm}23Ma(1{\sigma})$, and $221{\pm}7Ma(1{\sigma})$ were yielded from two spot analyses on the overgrowth rims of zircon. The rims are geochemically characterized by low Th/U ratios (<0.01) and strongly depleted light rare earth elements. The Permian-Triassic apparent ages of zircon are consistent with the $^{208}Pb/^{232}Th$ ages dated from allanite ($227{\pm}7Ma(t{\sigma})$) in the same sample within uncertainties, indicating an equilibrium growth of allanite and zircon at ~227 Ma. On the other hand, the younger $^{208}Pb/^{232}Th$ and $^{206}Pb/^{238}U$ ages ($213{\pm}4Ma(t{\sigma})$ and $186{\pm}9Ma(t{\sigma})$, respectively) of allanite may result from Pb loss due to the infiltration of alkali fluids from Late Triassic and Jurassic granitoids nearby.

Quantitative Analysis of Normal Salivary Gland Function using Salivary Scintigraphy (타액선 신티그라피를 이용한 정상 타액선기능의 정량분석)

  • Kim, Hui-Jin;Ko, Myung-Yun;Park, June-Sang
    • Journal of Oral Medicine and Pain
    • /
    • v.25 no.4
    • /
    • pp.345-353
    • /
    • 2000
  • This study was to investigate normal salivary flow rates and normal indices of Quantitative analysis of salivary scintigraphy. 96 adult volunteers were studied by Questionnaire evaluating salivary conditions and clinical examinations. 35(male 23, female 12, age range 23-31years) that absented subjective and objective symptoms related saliva were classified as normal group. The normal group underwent measurement unstimulated and stimulated salivary flow rates and salivary scintigraphy. The obtained results were as follows: 1. There were not significant in sex differences of unstimulated and stimulated salivary flow rates. The unstimulated salivary flow rate was $0.66{\pm}0.41g/min$, stimulated salivary flow rates was $1.61{\pm}0.69g/min$. 2. As comparing of parameters of salivary scintigraphy, the Uptake ratio(UR), $T_{max}$, $T_{min}$, Maximum accumulation (MA), Maximum secretion(MS) of parotid and submandibular glands were not significant in sex and side-ralated differences. 3. The UR, $T_{max}$, MA, MS of parotid gland were significantly higher than those of submandibular gland; in the parotid gland, UR, $3.67{\pm}0.88$, $T_{max}$, $18.77{\pm}0.43min$, MA, $41.35{\pm}9.22%$, MS, $43.13{\pm}9.13%$; in the submandibular gland, UR, $3.04{\pm}0.10$, $T_{max}$, $18.48{\pm}0.52min$, MA, $36.47{\pm}14.18%$, MS, $36.88{\pm}12.20%$. 4. As classifying of time-activity curve, the most of parotid gland was N-type(97.1%), submandibular gland was observed in order of M-type(67.1%), N-type(21.4%), F-type(11.4%), however, was not observed S-type. 5. As the type of time-activity curve of submandibular gland was more flattened, the UR, $T_{max}$, MA, MS were significantly decresed.

  • PDF

A Bottleneck Search Algorithm for Digraph Using Maximum Adjacency Merging Method (최대 인접 병합 방법을 적용한 방향 그래프의 병목지점 탐색 알고리즘)

  • Lee, Sang-Un
    • The Journal of the Institute of Internet, Broadcasting and Communication
    • /
    • v.12 no.5
    • /
    • pp.129-139
    • /
    • 2012
  • Given digraph network $D=(N,A),n{\in}N,a=c(u,v){\in}A$ with source s and sink t, the maximum flow from s to t is determined by cut (S, T) that splits N to $s{\in}S$ and $t{\in}T$ disjoint sets with minimum cut value. The Ford-Fulkerson (F-F) algorithm with time complexity $O(NA^2)$ has been well known to this problem. The F-F algorithm finds all possible augmenting paths from s to t with residual capacity arcs and determines bottleneck arc that has a minimum residual capacity among the paths. After completion of algorithm, you should be determine the minimum cut by combination of bottleneck arcs. This paper suggests maximum adjacency merging and compute cut value method is called by MA-merging algorithm. We start the initial value to S={s}, T={t}, Then we select the maximum capacity $_{max}c(u,v)$ in the graph and merge to adjacent set S or T. Finally, we compute cut value of S or T. This algorithm runs n-1 times. We experiment Ford-Fulkerson and MA-merging algorithm for various 8 digraph. As a results, MA-merging algorithm can be finds minimum cut during the n-1 running times with time complexity O(N).

울릉분지 돌고래 시추공의 생층서: 고환경 및 이들의 석유탐사에의 응용

  • 이성숙;윤혜수;배부영;박세진;이의형;강소라;김재호;김기현
    • 한국석유지질학회:학술대회논문집
    • /
    • autumn
    • /
    • pp.50-67
    • /
    • 1999
  • Biostratigraphic and paleoenvironmental analyses are carried out on cutting samples from the Dolgorae wells drilled in the Ulleung basin. The clayey, silty, and sandy sediments of the wells yield various microfossil assemblages of relatively good preservation, among which five fossil groups are reported; a total of 97 foraminiferal species of 66 genera, 19 nannofossil species of 12 genera, 86 ostracod species of 41 genera, 107 diatom species of 44 genera, and 124 dinoflagellate cysts species of 45 genera. Based on microfossils the geologic ages of the Dolgorae wells are dated to be from late Early Miocene to Early Pleistocene. Several biohorizons are defined in Neogene successions by the LOD (Last Ocurrence Datum) and FOD (First Ocurrence Datum) of marker species including G. truncatulinoides (LOD: 1.9 Ma) of foraminifera; C. macintyeri (LOD: 1.64-1.60), G. oceanica (FOD: 1.65 Ma), G. caribbeanica (1.72 Ma), D. brouweri (LOD: 2 Ma), R. pseudoumbilica (LOD: 3.66 Ma), P. lacunosa (FOD: 4.2 Ma) of nannofossils; S. ellipsoideus (LOD: 4 Ma), S. palcacantha (LOD: 10.2), C. giusepei (LOD: 14 Ma) of dinocysts; D. seminae v. fossilis (FOD: 3.7 Ma), T. antiqua (LOD: 1.7 Ma), T. convexa (LOD: 2.4 Ma), N. kamtschatica (LOD: 2.58 Ma), T. oestrupii(FOD: 5.1 Ma) of diatoms. Abundance patterns of microfossils throughout the wells reflect changes in paleoenvironmental and sedimentological settings of the basin in relation to sea-level variations. According to these data the large-cycle and small-cycle changes of transgression and regression phases are observed in terrestrial to marine sediments. This high-resolution sequence biostratigraphy established by various fossil groups enabled more reliable correlation between strata and refined interpretation on deposition systems of the basin. It also proved to provide fundamental and precise informations regarding stratigraphic correlation, tectonic events, basin, and depositional history for hydrocarbon explorations, especially in collaboration with seismic-stratigrahic analyses.

  • PDF