• Analytical Science and Technology
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• v.7 no.1
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• pp.25-31
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• 1994

As for analytes which did not represent the differences of the selectivity on the stationary and mobile phase, secondary chemical equilibrium theory was applied to study pH effects on the separation of alkylphenols. Mobile phase was consisted of an aqueous sodium carbonate-bicarbonate buffer and acetonitrile. The maximum selectivity for adjacent peak pairs was predicted from those values of $k^{\prime}_{HA}/k^{\prime}_{A-}$ and ${\Delta}pK$. The optimum pH determined by this method was 11.18 pH and solvent selectivity were considered at the same time to invoke the full range of selectivity effects possible for separations. Quaternary mixture composed of methanol, acetonitrile, tetrahydrofuran and water was adjusted to optimum pH 11.18. As the statistical simplex technique of an overlapping resolution map (ORM) was used to predict the optimized solvent system. The optimum solvent, which gives complete separation of alkylphenols, was determined as follws MeOH : ACN : THF = 14.4 : 81.8 : 2.8.

• Journal of The Korean Society of Agricultural Engineers
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• v.59 no.2
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• pp.1-11
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• 2017

The analysis of baseflow contribution is very significant in Korea because most rivers have high variability of streamflow due to the monsoon climate. Recently, the importance of such analysis is being more evident especially in terms of river management because of the changing pattern of rainfall and runoff resulted from climate change. Various baseflow separation methods have been developed to separate baseflow from streamflow. However, it is very difficult to identify which method is the most accurate way due to the lack of measured baseflow data. Moreover, it is inappropriate to analyze the annual baseflow contribution for Korean rivers because rainfall patterns varies significantly with the seasons. Thus, this study compared the baseflow contributions at various time-scales (annual, seasonal and monthly) for the 4 major river basins through BFI (baseflow index) and suggested baseflow contribution of each basin by the BFI ranges searched from different baseflow separation methods (e.g., BFLOW, HYSEP, PART, WHAT). Based on the comparison of baseflow contributions at the three time scales, this study showed that the baseflow contributions from the monthly and seasonal analysis are more reasonable than that from the annual analysis. Furthermore, this study proposes that defining BFI with its range is more proper than a specific value for a watershed, considering the difference of BFIs between various baseflow separation methods.

• Journal of Animal Reproduction and Biotechnology
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• v.36 no.2
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• pp.91-98
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• 2021

The objective of this study was to establish a selection process for high quality sperm in bovine semen using sperm separation by magnetic activation (MACS). For this, semen from 21 Nellore bulls was collected using an artificial vagina. To guarantee the presence of pathologies in the ejaculate, animals previously declassified in four consecutive spermiogram were used. Semen was analyzed in five statuses: (1) fresh semen (fresh); (2) density gradient centrifugation (DGC), percoll column; (3) non-apoptotic fraction after separation by MACS (MAC); (4) apoptotic fraction from the separation (MACPOOR); and (5) MAC followed by DGC (MACDGC). Using a computerized analysis system (CASA), motility was measured. The sperm morphology was evaluated by phase contrast, and the supravital test was completed with eosin/nigrosin staining. For DGC, 20 × 10⁶ cells were used in a gradient of 90% and 45% percoll. MACS used 10 × 10⁶ cells with 20 μL of nanoparticles attached to annexin V, and filtered through the MiniMACS magnetic separation column. Membrane integrity was assessed with SYBR-14/IP and mitochondrial potential with JC-1 by flow cytometry. Processing sperm by MACDGC, was more effective in obtaining samples with high quality sperm, verified by the total of abnormalities in the samples: 35.04 ± 2.29%, 21.50 ± 1.47%, 17.30 ± 1.10%, 30.68 ± 1.94% and 10.50 ± 1.46%, respectively for fresh, DGC, MAC, MACPOOR, and MACDGC. The subpopulation of non-apoptotic sperm had a high number of live cells (82.65%), membrane integrity (56.60%) and mitochondrial potential (83.98%) (p < 0.05). These findings suggest that this nanotechnological method, that uses nanoparticles, is efficient in the production of high-quality semen samples for assisted reproduction procedures in cattle.

• Korean Journal of Animal Reproduction
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• v.9 no.2
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• pp.105-112
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• 1985

This research was carried out to investigate the optimal conditions ssociated with the RIA procedures such as a bridging phenomena, prozone effects and a new separation methods etc. The results obtained were summarized as follows: 1. The lgG fractions of donkey anti-rabbit IgG sera were purified by Protein-A-Sepharose affinity column, which indicates that Protein-A an affinity for IgG class of donkey antiserum. 2. In coating the IgG fraction on polystryene tubes, incubation conditions made no differences between 2 hr at room temperature and overnight at 4$^{\circ}C$. 3. There were no significant differences between 1st antibody-coated tube and 2nd antibody-coated tube as a separation method when compared in terms of reproducbility. A better reproducibility may be expected if the titers of 1st antibody for the progesterone to be assayed and of corresponding 2nd antibody are reasonably high. 4. The titers of anti-progesterone antibody for 3H-progesterone and progesterone-11HS-125I were 1:300 and 1:700 in liquid-phase, and 1:100 and 1:300 in solid-phse for the separation methods. 5. A bridging phenomena in which a standard curve is long and shallow were observed when progesterone-11HS-125I was used for the tracer, but not in 3H-progesterone. 6. A prozone effect in a solid-phase system, especially 1st antibody-coated tube method was observed which the degree of inhibition was significantly different although zero bindings look the same. In this case, the titration curve should be made both in the absence and in the presence of a, pp.opriate amount of competiter, standard, respectively.

• The Journal of the Acoustical Society of Korea
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• v.40 no.5
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• pp.503-514
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• 2021

Reverberation in speech signals tends to significantly degrade the performance of the Blind Source Separation (BSS) system. Especially in online systems, the performance degradation becomes severe. Methods based on joint diagonalizability constraints have been recently developed to tackle the problem. To improve the quality of separated speech, in this paper, we add the proposed de-reverberation method to the online BSS algorithm based on the constraints in reverberant environments. Through experiments on the WSJCAM0 corpus, the proposed method was compared with the existing online BSS algorithm. The performance evaluation by the Signal-to-Distortion Ratio and the Perceptual Evaluation of Speech Quality demonstrated that SDR improved from 1.23 dB to 3.76 dB and PESQ improved from 1.15 to 2.12 on average.

• Journal of Microbiology and Biotechnology
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• v.17 no.11
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• pp.1751-1757
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• 2007

Elastin-like polypeptides (ELPs) undergo a reversible inverse phase transition upon a change in temperature. This thermally triggered phase transition allows for a simple and rapid means of purifying a fusion protein. Recovery of ELPs-tagged fusion protein was easily achieved by aggregation, triggered either by raising temperature or by adding salt. In this study, levansucrase has been used as a model enzyme in the development of a simple one-step purification method using ELPs. The levansucrase gene cloned from Pseudomonas aurantiaca S-4380 was tagged with various sizes of ELPs to functionally express and optimize the purification of levansucrase. One of two ELPs, ELP[V-20] or ELP[V-40], was fused at the C-terminus of the levansucrase gene. A levansucrase-ELP fusion protein was expressed in Escherichia coli $DH5{\alpha}$ at $37^{\circ}C$ for 18 h. The molecular masses of levansucrase-ELP[V-20] and levansucrase-ELP[V-40] were determined as 56 kDa and 65 kDa, respectively. The phase transition of levansucrase-ELP[V-20] occurred at $20^{\circ}C$ in 50 mM Tris-Cl (pH 8) buffer with 3 M NaCl added, whereas the phase transition temperature ($T_t$) of levansucrase-ELP[V-40] was $17^{\circ}C$ with 2 M NaCl. Levansucrase was successfully purified using the phase transition characteristics of ELPs, with a recovery yield of higher than 80%, as verified by SDS-PAGE. The specific activity was measured spectrophotometrically to be 173 U/mg and 171 U/mg for levansucrase-ELP[V-20] and levansucrase-ELP[V-40], respectively, implying that the ELP-tagging system provides an efficient one-step separation method for protein purification.

• The Journal of the Acoustical Society of Korea
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• v.38 no.4
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• pp.427-432
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• 2019

In this paper, we propose a home monitoring system using sound event detection based on a bidirectional gated recurrent neural network for the hard-of-hearing. First, in the proposed system, packet loss concealment is used to recover a lost signal captured through wireless sensor networks, and reliable channels are selected using multi-channel cross correlation coefficient for effective sound event detection. The detected sound event is converted into the text and haptic signal through a harmonic/percussive sound source separation method to be provided to hearing impaired people. Experimental results show that the performance of the proposed sound event detection method is superior to the conventional methods and the sound can be expressed into detailed haptic signal using the source separation.

• Journal of the Society of Naval Architects of Korea
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• v.58 no.2
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• pp.90-96
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• 2021

In this study, the method of determining the state of grid points in the adaptable surface particle method based on grid system developed as a free-surface tracing method was improved. The adaptable surface particle method is a method of determining the state of the grid point according to the shape of the free-surface and obtaining the intersection of the given free-surface and grid line where the state of the grid point changes. It is difficult to determine the state of grid points in the event of rapid flow, such as collision or separation of free-surfaces, and this study suggests a method for determining the state of current grid points using the state of surrounding grid points where the state of grid point are known. A grid layer value was assigned sequentially to a grid away from the free-surface, centering on the boundary cell where the free-surface exists, to identify the connection information that the grid was separated from the free-surface, and to determine the state of the grid point sequentially from a grid away from the free-surface to a grid close to the free-surface. To verify the improved method, a numerical analysis was made on the problem of dam break in which a sudden collision of free-surface occurred and the results were compared, and the results were relatively reasonable.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2000.10a
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• pp.665-668
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• 2000

This paper presents development of a different substance monitoring system base reliable detection between the conveyer belt and hopper used lot materials transport line of steel company. Conventional detection method of a piece of iron separation system is losed the confidence, because of the place with bad surroundings of measurement so much that materials Production line are completely exposed to dust, moisture and vibration. For the solution of this problem, we developed a different substance detection system using the acoustic emittion sensor and one chip microprocessor which is available for bad surroundings and inexpensive. The reliability of the system was estimated by experiment.

• Journal of Satellite, Information and Communications
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• v.7 no.1
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• pp.102-107
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• 2012

In this paper, we presented interference scenarios and an evaluation methodology for the co-frequency radio interference between a multi-beam mobile satellite communication system and a mobile communication system in 2.1 GHz band. Radio interferences between these systems are calculated using a minimum coupling loss method and an assesment for minimum separation distances was conducted for coexistence of a mobile satellite system and a mobile system in the same geographical area.