Korean Journal of Animal Reproduction (한국가축번식학회지)
- Volume 9 Issue 2
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- Pages.105-112
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- 1985
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- 1226-5284(pISSN)
Optimization of Immunosasay Procedures for the measurement of Progesterone
Progesterone 측정을 위한 면역분석법의 최적조건에 관한 연구
Abstract
This research was carried out to investigate the optimal conditions ssociated with the RIA procedures such as a bridging phenomena, prozone effects and a new separation methods etc. The results obtained were summarized as follows: 1. The lgG fractions of donkey anti-rabbit IgG sera were purified by Protein-A-Sepharose affinity column, which indicates that Protein-A an affinity for IgG class of donkey antiserum. 2. In coating the IgG fraction on polystryene tubes, incubation conditions made no differences between 2 hr at room temperature and overnight at 4
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