• 한국식품영양과학회지
• /
• 제28권1호
• /
• pp.274-276
• /
• 1999

Sardine oil was microencapsulated by spray drying method in wall systems containing corn syrup in combination with sodium casein or wheat protein. Analysis of microencapsulation yield of sardine oil was carried out by a modified soxhlet method which could reduce the extraction time of surface oil. Microencapsulation yield of sardine oil was ranged from 65.3 to 93.5 % depending on the sodium casein content.

• 동아시아식생활학회지
• /
• 제10권5호
• /
• pp.439-444
• /
• 2000

호알칼리성 방선균 Streptomyes sp. B-2를 Glucose Isomerse 생성을 위해 토양에서 분리했다. Glucose Isomerase(G.I)는 high fructose glucose syrup과 fructose의 생산을 위해서 식품 공업에서 아주 중요시되고 있는 효소이다. 호알칼리성 방선균 Streptomyces sp. B-2가 생성하는 glucose isomerase(G.I.)를 정제하였다. G.I.는 (NH$_4$)$_2$So$_4$분획, DEAE-cellulose, Sephadex G-200 chromatography하여 순수 분리 하였다. 순수분리된 G.I.는 electrophoresis에 의해 확인을 했다. SDS-acrylamide gel electrophoresis에 의해 정제된 효소는 single band를 보여주었다.

• 미생물학회지
• /
• 제21권2호
• /
• pp.51-60
• /
• 1983

Cultural characteristics of Strptomyces griseolus isolated from the soil were investigated. This strain was disclosed to utilize D-xylose, and D-glactose in preference order as a carbon source with the formation of glucose isomerase. The addition of sweet potato starch also proved effective promoting the total enzyme activity measured at 29% higher than the control. Corn cob, one of waste agricultural resources, was hydrolyzed in 2~3% $H_2SO_4$ solution at $100^{\circ}C$, 3~5 hours to produce a xylose syrup which gave rise to the recovery of 19.9% in a batch system and 28.2% in a repeated system. By the addition of both 2% of xylose syrup(Be'28) prepared by and us 65% of corn steep liquor (total nitrogen 1.2%), enzyme induction was maximized. The enzyme activity was stimulated by the xylose and the cell growth by the C.S.L. Also, remarkable increase of enzyme activity was noticed by the addition of protein acid hydrolysate 86.2% higher than the control. $QO_2$ of the biomass cultured in 30L capacity jarfermentor recorded low oxygen requirement of 251.2 1/hr. Maximum activity of glucose isomerase was observed noted at the 9th hour after inoculation which is 2 hours faster than the stationery was observed noted at the 9th hour after inoculation which is 2 hours faster than the stationery phase of the biomass growth. Glucose isomerase from the strain was activated by adding the $Co^{++}\;and\;Mg^{++}$ with optimum temperature of $73^{\circ}C$ and pH of 7.2. Conversion ratio of 60% glucose to frutose was 42.5% after 70 hours reaction.

• 한국식품조리과학회지
• /
• 제11권2호
• /
• pp.93-97
• /
• 1995

한국 고유의 후식인 약과에 생강즙을 첨가하여 관능검사와 저장에 의한 지방의 산패를 조사하였다. 생강즙의 첨가량은 밀가루 1cup에 0tea spoon(t.s)에서 2t.5이 반죽으로 가능하였으며, 질감, 모양, 색에서는 유의적인 치지를 나타내지 않았으나, 향 맛, 전체적인 선호 In에서 는 유의적인 차이를 나타내었다. 생강즙 1t.5, 1.51 j, 2.0 1.5를 첨가한 약과들은 위의 세가지 평가 부문에j, 높은 점수를 보였으며, 이들 사이에서는 향에 대한 평가만을 제외하고는 유의적인 차이를 나타내지 않았다. 생강즙의 첨가는 튀김과 저장시 일어나는 지방산화를 억제시키는 효과를 보였으며, 이 효과는 생강즙의 첨가량이 증가함에 따라 비례적으로 증가하였다. 또한 시럽이나 꿀로 집청하여 저장한 약과가 집청하지 않고 저장한 약과보다 지방의 산화가 억제되었다. 이 효과는 꿀로 집청한 약과가 시럽으로 집청한 약과보다 크게 나타났다. 그러므로 생강즙의 첨가와 시럽이나 꿀로 잡청함으로써 약과를 상온에서도 좋은 품질로 유지하면 서 보존할 수 있을 것으로 생각된다.

• 한국식품과학회지
• /
• 제26권6호
• /
• pp.659-664
• /
• 1994

제주 전래 맥아엿의 최적당화조건을 찾기 위하여 곡류, 당화온도 및 시간에 따른 가용성 고형분($^{\circ}{Brix}$), DE, 환원당 및 유리당 변화를 조사하였다. 가용성 고형분에서 찹쌀은 약 $20^{\circ}$Brix, 차조늘 약 $17^{\circ}$Brix에서 평형에 도달하였으며 혼합곡류는 찹쌀과 비슷한 경향을 나타내었다. 찹쌀은 $50^{\circ}C$, 차조는 $55^{\circ}C$에서 3시간 당화조건이 적정 당화조건으로 판단되었다. HPLC에 의한 유리당분석 결과 maltose는 $10{\sim}$13%이며 glucose는 1% 정도이고, fructose포 미량 검출되었다. $^{\circ}{Brix}$와 환원당과의 상관관계는 우수하여(r=0.954) 당화종점을 $^{\circ}{Brix}$의 변화에 의하여 측정하는 것이 가능하였다.

• Clinical and Experimental Pediatrics
• /
• 제49권11호
• /
• pp.1125-1139
• /
• 2006

In 1991, the Ministry of Health & Social affairs adopted a nationwide service program for neonatal screening of phenylketonuria, galactosemia, maple syrup urine disease, homocystinuria, histidinemia & congenital hypothyroidism for newborns delivered from low class pregnant women registered in health centers. Government decreased the test items from six to two, PKU & congenital hypothyroidism to increase test numbers with same budget from 1995. Government decided to test PKU & hypothyroidism for all newborns from 1997. 78 laboratories wanted to participate for neonatal screening test in 1999. Government didn't decide laboratory center for a certain district and placed responsibility on free competition. Government are planning to test 573,000 newborns from 1998, Government decided to screen 6 items PKU, congenital hypothyroidism, maple syrup urine disese, homocystinuria, galactosemia and congenital adrenal hyperplasia from 2006. 17 laboratores are participating now. The cost of screening test is supported by both the federal government and local government on a 40-60 basis. In case a patient with an inherited metabolic disease is diagnosed by screening of government program, special milk is provided at government's expense. Interlaboratory quality control was started 6 times a year from 1994. According to the government project, 3,707,773 newborns were screened. 86 PKU, 718 congenital hypothyroidism were detected. So incidence of PKU is 1/43,114 and congenital hypothyroidism is 1/4,612. Maeil dairy company produced new special formula for PKU, MMA and PA, MSUD, urea cycle disorder, homocystinuria, isovaleric acidemia from Oct. 1999. The cost benefit of performing screening procedures coupled with treatment has been estimated to be as high as 1.77 times in PKU, 11.11 times in congenital hypothyroidism than cost without screening. We are trying to increase the budget to test all newborns for Tandem mass sereening & Wilson disease from 2008. Now it is a very important problem to decrease laboratory numbers of neonatal screening in Korea. So we are considering 4-5 central laboratories which cover all newborns and are equipped with tandem mass spectrometer & enzyme immunoassay for TSH, 17OHP & enzyme colorimetric assay for galactose.

• 한국발생생물학회지:발생과생식
• /
• 제21권2호
• /
• pp.151-156
• /
• 2017

High-fructose corn syrup (HFCS) is widely used as sweetener, and its overconsumption is become a major health problem. In the present study, we used adult female rats and applied a 28 days HFCS feeding model to monitor the estrous cycle and changes in tissue weights and histology. Adult female rats were divided into three groups. Animals were fed with ad libitum normal chow and (1) 24 hours tap water (Control group), (2) 12 hours HFCS access during dark period and 12 hours tap water (12H group), and (3) 24 hours HFCS only access (24H group). Total exposure period was 28 days. There is no significant change in body weight between control and HFCS-fed animals. Both absolute and relative weights of ovary in 24H animals were significantly heavier than those in control or 12H animals. The absolute and relative weights of the kidney and liver in 24H groups were significantly heavier than those in control or 12H animals. The estrous cycles of the 24H animals were significantly longer. Histological analyses revealed that 24H ovaries were relatively bigger and possessed more corpus lutea than control ovaries. Uterine sections of 12H and 24H animals showed a well-developed stratum vasculare between inner and outer myometrial layers. The number of endometrial glands were decreased in 12H uteri, and recovered in 24H uteri compared to control. Numbers of convoluted tubule in distal region increased in 12H and 24H kidney samples. Liver specimens of 12H and 24H showed the increased number of fat containing vacuoles. In conclusion, our study demonstrated that HFCS treatment for 28 days could induce (1) changes in length of estrous cycle with extended estrous and diestrous stages, (2) altered ovarian and uterine histology, and (3) liver and renal lipid accumulation. These findings reveal the adverse effects of HFCS drinking on the reproductive function and lipid metabolism of female rats.

• Journal of Dairy Science and Biotechnology
• /
• 제35권2호
• /
• pp.99-104
• /
• 2017

The global consumption of fructose in the form of high-fructose corn syrup (HFCS) has increased considerably over the past several decades. The current intake of HFCS exceeds that of other major caloric sweeteners such as sucrose. Fructose has potentially adverse effects on human metabolism. Therefore, the objective of this study was to analyze the monosaccharide contents of 13 sweetened milk-based beverages by using high-performance liquid chromatography (HPLC). The total monosaccharide (fructose and glucose) contents of various sweetened milk-based beverages ranged from 0.60 g/100 mL (cheese) to 26.03 g/100 mL (maple caramel snack), while milk showed only 0 g/100 mL monosaccharides. The fructose-to-glucose ratio of various popular sweetened milk-based beverages ranged from not-detected (ND) to 2.24 g/100 mL, but the content of glucose was higher than that of fructose in only three samples, namely, strawberry flavored milk, shrimp chips, and maple caramel snack. The analyzed results revealed important basic information about monosaccharides in various milk-based sweetened beverages, presenting ideas for future fructose research.

• 한국식품조리과학회지
• /
• 제13권5호
• /
• pp.609-616
• /
• 1997

한국 대표적인 기호식품의 하나인 약과를 저장시 지방의 산패정도와 물성을 평가하였다. 15$0^{\circ}C$에서 8분 동안 튀겨서 10분간 집청한 약과를 표준시료로 하여 분석한 결과 지방은 24.4%로 매우 높은편이었다. \circled1저장시 수분함량은 시럽집청한 약과가 9.9~10.8% 로서 집청하지 않은 구에 비해 약 4%정도 높았다 \circled2 0, 5, 10, 15, 20, 30일간 상온저장하면서 측정한 산가, TBA가, 카보닐가는 저장초기에 비해 저장 30일에는 각각 2배, 1.3배, 1.4배로 증가하엿다. \circled3 집청은 산패의 진행을 방지하는 효과를 보였고, 생강즙첨가시는 다소 낮은 산가, TBA가, 카보닐가를 나타내어 집청과 생강즙의 첨가는 약과 저장시의 지질산패 억제에 효과가 있었다. \circled4색도의 L값은 집청구가 집청하지 않은 구에 비해 낮았고 저장함에 따라 감소하였으며, a, b값은 집청하지 않은 구가 다소 높았다. \circled5저장시의 rheometer 측정에서는 생강즙을 넣고 시럽에 집청한 약과가 다른 실험구에 비해 elasticity와 chewiness가 다소 감소하였으나, 저장기간이나 생강즙 첨가유무는 질감에 영향을 미치지 못하였고 집청이 큰 요인이었다.

• Advances in concrete construction
• /
• 제9권6호
• /
• pp.547-556
• /
• 2020

This study aimed to develop reference materials (RMs) that are chemically stable and can simulate the flow characteristics of cement paste. To this end, the candidate components of RMs were selected considering the currently required properties of RMs. Limestone, slag, silica, and kaolin were selected as substitutes for cement, while glycerol and corn syrup were selected as matrix fluids. Moreover, distilled water was used for mixing. To select the combinations of materials that meet all the required properties of RMs, flow characteristics were first analyzed. The results revealed that silica and kaolin exhibited bilateral nonlinearity. When an analysis was conducted over time, slag exhibited chemical reactions, including strength development. Moreover, fungi were observed in all mixtures with corn syrup. On the other hand, the combination of limestone, glycerol, and water exhibited a performance that met all the required properties of RMs. Thus, limestone, glycerol, and water were selected as the components of the RMs. When the influence of each component of the RMs on flow characteristics was analyzed, it was found that limestone affects the yield value, while the ratio of water and glycerol affects the plastic viscosity. Based on this, it was possible to select the mixing ratios for the RMs that can simulate the flow characteristics of cement paste under each mixing ratio. This relationship was established as an equation, which was verified under various mixing ratios. Finally, when the flow characteristics were analyzed under various temperature conditions, cement paste and the RMs exhibited similar tendencies in terms of flow characteristics. This indicated that the combinations of the selected materials could be used as RMs that can simulate the flow characteristics of cement paste with constant quality under various mixing ratio conditions and construction environment conditions.