• Title/Summary/Keyword: synthesis variation

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Tissue Specific Gene Regulation of The Anthocyanin Synthesis Regulator Gene R in Maize (옥수수의 색소 발현에 관련된 조직 특이성 조절유전자 R locus에 관하여)

  • 임용표
    • Proceedings of the Botanical Society of Korea Conference
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    • 1987.07a
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    • pp.323-347
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    • 1987
  • The R locus of maize in one of several genes that regulate the anthocyanin pigments throughout the body of the plant and seed. The R gene product may regulate pigment deposition by controlling the expression of the flavonoid biosynthetic gene pathway in a tissue-specific manner. To understand the basis for tissue specific regulation and allelic variation at R, the molecular study has been done by cloning a portion of the R complex by transposon tagging with Ac. R specific probe were cloned from the R-nj mutant induced by Ac insertion mutagenesis. From southern analysis of R-r complex using the R-nj probe, the structure of R-r was proposed that R-r containes the three elements, (P)(Q)(S). These elements may organize as the inversion triplication model which (S) sequence was inverted in relation to (P) and (Q). The R-sc derivated from R-mb or R-nj was cloned with R-nj probe, and molecular genetical data showed that R-sc containes tissue specific and tissue nonspecific area, and the sequencing of R-sc are progressed now.

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Synthesis of YBCO Superconducting Precursor using Organic metal salts method for Electric power transmission (유기금속염을 이용한 전력 전송용 YBCO 초전도 전구체 합성)

  • Lee, Sang-Heon
    • Proceedings of the KIEE Conference
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    • 2005.07c
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    • pp.1966-1967
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    • 2005
  • The solid precursor produced by the dehydration of the gel at $120^{\circ}C$ for 12h is not in the amorphous state as expected but in a crystalline state. X-ray diffraction peaks of nearly the same angular position as the peaks of high Tc phase were observed in the precursor. Experimental results suggest that the intermediate phase formed before the formation of the superconducting phase may be the most important fact in determining whether it is easy to form the superconducting phase or not, because the nucleation barriers of the two superconducting phase may be altered by the variation of the crystal structures of those intermediate phase.

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Electrochemical Properties of LiMn$_2$O$_4$by the Sort of Mixing Materials and Variation of Mole ratios (합성 물질의 종류 및 몰비 변화에 따른 LiMn$_2$O$_4$의 전기화학적 특성)

  • 최형기;박현배;정인성;구할본
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 1997.11a
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    • pp.286-289
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    • 1997
  • It was studied that the effect of the mixing materials and the mole ratios on electrochemical properties of LiMn$_2$O$_4$LiMn$_2$O$_4$is prepared by reacting stoichiometric mixture of LiOH.$H_2O$ and MnO$_2$(EMD or CMD) and heating at 80$0^{\circ}C$ for 36h. We obtained properties of crystal structure through X-ray diffraction. LiMn$_2$O was reversible at 4.5V~3.0V and displayed two reduction and oxidation. Optimum synthesis results were obtained by reacting with LiOH.$H_2O$ and MnO$_2$(EMD) at mole ratio 1:2.

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Synthesis of Permalloy (Ni-Fe) Nanosheets through Sonoelectrochemical Methods and its Magnetic Properties

  • Rhee, Ryan;Moon, Kyounghoon;Yoo, Bongyoung
    • Journal of the Korean institute of surface engineering
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    • v.51 no.4
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    • pp.202-206
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    • 2018
  • Permalloy($Ni_{80}-Fe_{20}$) which is known for its soft magnetic properties is a well-known material that has been studied intensively. Permalloy nanoflakes were fabricated with the combination of electrodeposition and sonication process. Ultrasonic power was applied to the deposited alloy which produced nanoflakes in forms of sheet. High internal stress created cracks which helped the peeling of permalloy into nanosheets. Because of shape anisotropy, flakes could be aligned by magnetic field. The magnetic properties of the nanosheets were observed, and the variation of magnetic properties with the alignment of flake was also investigated.

Sequential Copolypeptides (Ⅲ). Synthesis and Characterization of Poly ($\gamma$-benzyl-L-glutamyl-$\gamma$-benzyl-L-glutamyl-glycine)

  • Choi, Sang-Up;Kang, Joon-Kil
    • Bulletin of the Korean Chemical Society
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    • v.1 no.3
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    • pp.78-82
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    • 1980
  • Solutions of $Cd^{2+}$, $Co^{2+}$ and $Ni^{2+}$ were mixed with the solutions of hydroxycarboxylic acids such as salicylic, lactic and mandelic acids in the presence of cation exchange resin at room temperature. The distribution ratios of the metal ions between resin and solution were measured, using radioactive metal ions as tracer. From the observed variation of the distribution ratios with the acid anion concentrations, it was concluded that $Cd^{2+}$, $Co^{2+}$ and $Ni^{2+}$ formed the one-to-one complexes with salicylate, lactate and mandelate ions in aqueous, 20 % ethanol-water and 20 % acetone-water solutions. The results of the present study indicated that the relative stabilities of the metal-acid complexes in solution increased in the order: $Cd^{2+}$ <$Co^{2+}$ <$Ni^{2+}$ complexes. Salicylate

In Situ Microfluidic Synthesis of Monodisperse PEG Microspheres

  • Choi, Chang-Hyung;Jung, Jae-Hoon;Hwang, Taek-Sung;Lee, Chang-Soo
    • Macromolecular Research
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    • v.17 no.3
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    • pp.163-167
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    • 2009
  • This study presents a microfluidic method for the production of monodisperse poly(ethylene glycol) (PEG) microspheres using continuous droplet formation and in situ photopolymerization in microfluidic devices. We investigated the flow patterns for the stable formation of droplets using capillary number and the flow rate of the hexade-cane phase. Under the stable region, the resulting microspheres showed narrow size distribution having a coefficient of variation (CV) of below 1.8%. The size of microspheres ($45{\sim}95{\mu}m$) could be easily controlled by changing the interfacial tension between the two immiscible phases and the flow rates of the dispersed or continuous phase.

Removal Characteristic of Ammonia Nitrogen and Behavior of Nitrogen in Synthetic Wastewater Using Leclercia Adecarboxylata (Leclercia Adecarboxylata를 이용한 합성폐수의 암모니아성질소 제거특성 및 질소거동)

  • Lee, Hyun-Hee;Phae, Chae-Gun
    • Journal of Korean Society of Environmental Engineers
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    • v.29 no.4
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    • pp.460-465
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    • 2007
  • In this study, the removal characteristic of ammonia nitrogen and behavior of nitrogen was investigated using Leclercia adecarboxylata, which was derived from the culture contaminated by ammonia nitrogen of high concentration. The method of ammonia nitrogen removal was not biological nitrification and denitrification but elimination of nutrient salt with internal synthesis of microorganisms which use ammonia nitrogen as substrate. L. adecarboxylata(one of ammonia synthesis microorganisms) was highly activated and showed the most high removal efficiency in free salt condition but the removal efficiency decreased badly in salt concentration of more than 4%. About 80 mg/L of $NH_3-N$ was mostly removed within 20 hours and 500 mg/L of $NH_3-N$ showed less then removal efficiency of 50% because carbon source was not enough. However, ammonium nitrogen concentration was decreased again when the carbon source was inserted additionally thus, ammonium nitrogen removal efficiency by L. adecarboxylata, was related to amount of carbon source. pH decreased from 8.0 to 6.36 according to growth of L. adecarboxylata. Concentration of nitrite nitrogen and nitrate nitrogen did not increase and TKN concentration showed no variation while ammonia nitrogen was removed by L. adecarboxylata. In addition to, when content of protein in organic nitrogen was measured, protein was not detected at the beginning of microorganism synthesis but protein of 193.1 mg/L was detected after 48 hours. Hence, ammonium nitrogen was not decomposed as nitrate nitrogen and nitrite nitrogen but synthesized by L. adecarboxylata, which has excellent ability of nitrogen synthesis and can threat ammonia nitrogen of high concentration in wastewater.

Cloning and Expression Analysis of the ${\alpha}$-Subunit of Porcine Prolyl 4-hydroxylase

  • Cho, Eun Seok;Jung, Won Youg;Kwon, Eun Jung;Park, Da Hye;Chung, Ki Hwa;Cho, Kwang Keun;Kim, Chul Wook
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.11
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    • pp.1655-1661
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    • 2007
  • Prolyl 4-hydroxylase (P4H) plays a central role in collagen synthesis by catalyzing the hydroxylation of the proline residue in the X-Pro-Gly amino acid sequence, and controls the biosynthesis of collagen that influences overall meat quality. In order to verify expression level of the catalytic ${\alpha}$ subunit of P4H, a 2.7 kb clone of the ${\alpha}$ subunit gene for P4H was selected from a cDNA library prepared from the muscular tissue of Sancheong berkshire pigs, and the whole gene sequence was determined. As expression level of the ${\alpha}$ subunit of P4H differed between tissues of pigs, we intended to assess more precisely the level of ${\alpha}$-subunit expression between tissues of Sancheong Berkshire pigs by using RT-PCR. Muscular and adipose tissues were taken from each pig grouped by growth stage (weighing 60, 80, and 110 kg) of Yorkshire and Sancheong Berkshire pigs, and the expression levels of the ${\alpha}$-subunit of P4H were examined. Since there were significant differences in the expression level with respect to variation in growth stage (p<0.01), an attempt was made to identify any influences of pig species and tissue variation. The muscular and adipose tissues of pigs weighing 110 kg showed higher expression levels than pigs weighing 60 kg and 80 kg. In general, significantly higher expression levels were found in muscular than in adipose tissue. The expression levels in Sancheong Berkshire were significantly higher than in Yorkshire pigs (p<0.01 or p<0.05). Since expression level of the ${\alpha}$-subunit of P4H affects the activity of P4H and is connected to the biosynthesis of collagen and increased collagen can improve meat texture, this finding may explain why meat quality of the Sancheong Berkshire pig is acclaimed in Korea. Given the higher expression levels of the ${\alpha}$-subunit gene in adipose than in muscular tissue, and also in the heavier pigs, more intensive studies are required to assess the correlation between expression level of the ${\alpha}$ subunit gene and overall meat quality.

QUANTIFICATION OF THE EFFECTIVENESS OF VESSEL TRAFFICSERVICES

  • 박진수
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 1994.10a
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    • pp.49-58
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    • 1994
  • The methods of estimation of VTS effectivenss are compared and the worldwide literature related to the VTS effectiveness is reviewed. The review suggests three potential approaches ; simulation ; synthesis of expert opinion and statistical analysis of casualties. this study adopted dissimilar approaches to estimate the VTS effectiveness to the earlier studies ; the combination of synthesis of expert opinion and causal analysis of casualty. The VTS effectiveness is derived by multiplying casualty rate reduction factors by the effect level of causal factors. The development of casualty rate reduction factors was based on the questionnaire survey and the evolution of effect levels was based on the causal analysis using functional block diagram. According to these procedures the maximum benefit to be obtained through the introduction of a VTS system was approximately 46 percent overall. The collision reduction rate was estimated to be approximately 50 percent for a VTS system with advanced radar surveillance. And 47 percent of groundings 36 percent of rammings and 21 percent of founderings could be reduced by the introduction of VTS. These figures are more or less the same to the earlier studies. The VTS effectiveness by the different causal factor groups was examined. VTS may reduce about 68 percent of causal factors classified as environmental conditions 40 percent of human factors and 35 percent of technical factors in collision accidents. As a whole 60 percent of environmental factors 41 percent of human factors and 20 percent of technical factors may be prevented by a VTS. The key variable of the effectiveness percentage is the value of weight coefficient $\delta$. Therefore differing values for this input was discussed and the impact that these variations have on the VS effectiveness noted. As the results of sensitivity analysis of VTS effectiveness by $\pm$10 percent the effectiveness is varied approximately three to seven percent by casualty type. And the value is changed roughly four to eight percent by a $\pm$10 percent variation by different sub-areas.

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Synthesis of $LiCoO_{2}$ powders from precursors prepared by precipitation process

  • Park, Cheong-Song;La, Jung-In;Kim, Do-Youn
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.12 no.2
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    • pp.87-90
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    • 2002
  • $LiCoO_{2}$ powders were synthesized at various temperatures using lithium hydroxide and cobalt hydroxide as precursors prepared by precipitation process and freeze-drying. In this study, the$LiCoO_{2}$ samples were synthesized via a solid state reaction with various LiOH concentration between 10 % and 30 % excess. And $LiCoO_{2}$powders were calcined at 600~$800^{\circ}C$ in a short time. Measurements of XRD and SEM were performed to characterize the properties of the prepared materials. The effect of amount of Li ions on the structural change in powder has been examined using the XRD analysis. For the not added excess of LiOH, CoOOH phase presented in the XRD pattern of $LiCoO_{2}$ due to loss of Li ions during firing. The morphology and particle size of the powders were examined using SEM. The obtained powders are high temperature-$LiCoO_{2}$HT-LiCoO$_{2}$) and homogeneous with the range of grain size in the order of hundreds of nanometers. The effects of variation of LiOH concentration on the structural change in powder were investigated using the Rietveld analysis. As an analysis result, c/a is constant by 4.99 on all occasions. Finally, the structure of HT-$LiCoO_{2}$ was simulated by the commercial software $Creius^{2}$(Molecular Simulations, Inc.) from the results of Rietveld analysis.