• Journal of Food Hygiene and Safety
• /
• v.10 no.3
• /
• pp.189-197
• /
• 1995

Analytical method for synephrine and octopamine in citrus fruits, drinks containing citrus fruit, and human urine was developed using gas chromatography / mass spectrometry(GC/MS), Silylation with MSTFA, acetylation with MBTFA, and trimethylsilylation with MSTFA followed by trifiuoroacetylation with MBTFA were compared. The selective derivatization of synephrine and octopamine was optimized with two derivatizing reagents ; MSTFA and MBTFA. The ion at m/z 267 was monitored to characterize the benzyl group of the both compounds. Synephrine was detected in the concentrations of 0.46∼1.88 ug/g for citrus fruits and 1.2∼8.1 ug/ml for drinks. The urinary excretion data of synephrine showed the highest concentration at the period of 8-20 hours after drinking orange juices and total amounts of its urinary excretion calculated as a parent compound was 11-14% of a dose during 48 hours. Octopamine was not detected in citrus fruits, drinks, and human urine.

• Korean Journal of Food Science and Technology
• /
• v.46 no.3
• /
• pp.276-282
• /
• 2014

An accurate and sensitive analytical method was established for the simultaneous determination of synephrine and n-methyltyramine contents by ultra-performance liquid chromatography (UPLC) with a fluorescence detector (FLD). A 70:30 (v/v) mixture of 10 mM sodium dodecyl sulfate (SDS) and acetonitrile was used as the mobile phase. The coefficient of correlation ($r^2$) was 0.9999 for both synephrine and n-methyltyramine, and their limits of detection (LOD) were 0.02 and 0.01 mg/kg, respectively. The percentage recoveries for synephrine and n-methyltyramine were 96.4% and 100.9%, respectively, from bitter orange (Citrus aurantium) samples. The synephrine and n-methyltyramine contents were 38.07-118.21 mg/kg and 0.27-0.56 mg/kg, respectively, in the orange fruit samples, while they were 14.61-120.39 mg/kg and up to 3.34 mg/kg, respectively, in the tested commercial orange juice samples. The differences in synephrine and n-methyltyramine content between orange fruit and commercial orange juice were not significant (p<0.05). These results suggest that UPLC-FLD can be applied to develop an analytical method of quality control for commercial orange juice.

• The Korean Journal of Pharmacology
• /
• v.10 no.2
• /
• pp.31-37
• /
• 1974

Citrus unshiu Marc., a family of Rutaceae, has been used as antitussives, antiemetics, expectorants, spasmolytics, stomachics and digestants in oriental medicine. But there is few reports on the pharmacological effects of Citrus unshiu pericarpium. Present experiment was performed to observe the effects of the water extract of Citrus unshiu on isolated auricle and intestine in rabbits and blood pressure in rats and also to study on the active component on the cardiovascular system by means of thin layer chlomatography and spectrophotometer. 1. The water extract showed positive inotropic and positive chronotropic effect in the concentration of $1{\times}10^{-4}g/ml$, $5{\times}10^{-4}g/ml$, and $1{\times}10^{-3}g/ml$. 2. The motility of isolated rabbit intestine was significantly depressed by the water extract. 3. The blood pressure was markedly increased by the water extract. 4. The blood vessel of rabbit ear was constricted by perfusion of the ether extract. 5. The purified alkaloid of Citri pericarpium by ion exchange resin showed the same Rf value with l-synephrine on thin layer chromatography. 6. The purified alkaloid of Citri pericarpium by thin layer chromatography showed the same absorption curve with l-synephrine in the ultraviolet scanning.

• Journal of Medicine and Life Science
• /
• v.15 no.1
• /
• pp.6-11
• /
• 2018

Previous studies have suggested that Citrus fruits might suppress the proliferation of various cancer cells. However, little is known about any specific ingredients in the extract of Citrus fruits to exert its anti-proliferative activity in cancer cells. The present study aimed to identify the active ingredients in Citrus fruits to suppress the proliferation of rat hepatocellular carcinoma cells. Among tested compounds, two polymethoxylated flavones (nobiletin and tangeritin) showed significant anti-proliferative activity whereas other compounds (synephrine, rutin, hesperidin) did not. Interestingly, nobiletin as well as tangeritin also decreased the protein amount of gluconeogenic enzymes, PEPCK and G6Pase. The possible involvement of gluconeogenic activity in the proliferation of hepatocellulacarcinoma cells are further to be investigated.

• Korean Journal of Pharmacognosy
• /
• v.33 no.1 s.128
• /
• pp.57-63
• /
• 2002

The purpose of this research was to investigate the effects of dried orange peel compound intaking and exercise training on the physique, body fat, and concentration bloodlipid in obese women. Ninety-seven volunteers(mean age 21.47 ${\pm}$ 1.47 yrs), all over 30% of body fat, agreed to Participate and were placed in 4 groups: dried orange peel compound intaking and exercise group(n=25), only exercise group(n=25), only dried orange peel compound intaking group(n=24), and control group(n=20). The subjects who joined this project were asked to take dried orange peel compound(2 times/day, 30 ml/time) or participate in exercise program(3 times/week, 70 min/day), depending on particular group for 12 weeks. They were tested on their height, weight, circumference of chest, abdomen, hip, and thigh, body fat%, total-cholesterol(TC), HDL-C, LDL-c, and triglyceride(TG) at the beginning and the end of the 12-weeks program. For data analysis, paired t-tests and ANCOVAs were used. The intaking of dried orange peel compound was influenced on weight, circumference of chest, abdomen, hip, and thigh, body fat%, HDL-C, LDL-C, and TG. The exercise participation had almost same effects on those dependent variables. Furthermore, the dried orange peel compound intaking along with exercise training was the most effective on those dependent variables among them. Therefore, these results indicate that dried orange peel compound intaking, like exercise, may be useful for controling weight, body fat, HDL-C, LDL-C, and TG.