Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Analysis of Synephrine and Octopamine in Citrus Fruits, Drinks, and Human Urine

Citrus Fruits, 드링크류, 소변중 Synephrine과 Octopamine의 분석에 관한 연구

  • 노동석 (한국과학기술연구원 도핑콘트롤센터) ;
  • 이정애 (한국과학기술연구원 도핑콘트롤센터) ;
  • 김승기 (한국과학기술연구원 도핑콘트롤센터) ;
  • 정현숙 (한국과학기술연구원 도핑콘트롤센터) ;
  • 유보경 (한국과학기술연구원 도핑콘트롤센터) ;
  • 박종세 (한국과학기술연구원 도핑콘트롤센터)
  • Published : 1995.09.01
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Abstract

Analytical method for synephrine and octopamine in citrus fruits, drinks containing citrus fruit, and human urine was developed using gas chromatography / mass spectrometry(GC/MS), Silylation with MSTFA, acetylation with MBTFA, and trimethylsilylation with MSTFA followed by trifiuoroacetylation with MBTFA were compared. The selective derivatization of synephrine and octopamine was optimized with two derivatizing reagents ; MSTFA and MBTFA. The ion at m/z 267 was monitored to characterize the benzyl group of the both compounds. Synephrine was detected in the concentrations of 0.46∼1.88 ug/g for citrus fruits and 1.2∼8.1 ug/ml for drinks. The urinary excretion data of synephrine showed the highest concentration at the period of 8-20 hours after drinking orange juices and total amounts of its urinary excretion calculated as a parent compound was 11-14% of a dose during 48 hours. Octopamine was not detected in citrus fruits, drinks, and human urine.

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