• Biodesign
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• v.5 no.4
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• pp.136-140
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• 2017

Lam6 is a member of sterol-specific ${\underline{l}ipid$ transfer proteins ${\underline{a}}nchored$ at ${\underline{m}ebrane$ contact sites (LAMs). Lam6 localizes to the ER-mitochondria contact sites by its PH-like domain and the C-terminal transmembrane helix. Here, we purified and crystallized the Lam6 PH-like domain from Saccharomyces cerevisiae. To aid crystallization of the Lam6 PH-like domain, T4 lysozyme was fused to the N-terminus of the Lam6 PH-like domain with a short dipeptide linker, GlySer. The fusion protein was crystallized under the condition of 0.1 M HEPES-HCl pH 7.0, 10% (w/v) PEG 8000, and 0.1 M $Na_3$ Citrate at 293K. X-ray diffraction data of the crystals were collected to $2.4{\AA}$ resolution using synchrotron radiation. The crystals belong to the orthorhombic space group $P2_12_12_1$ with unit cell parameters $a=59.5{\AA}$, $b=60.1{\AA}$, and $c=105.6{\AA}$. The asymmetric unit contains one T4L-Lam6 molecule with a solvent content of 58.7%. The initial attempt to solve the structure by molecular replacement using the T4 lysozyme structure was successful.

• The Bulletin of The Korean Astronomical Society
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• v.44 no.1
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• pp.49.1-49.1
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• 2019

Giant radio relics in the outskirts of galaxy clusters have been observed and they are interpreted as synchrotron emission from relativistic electrons accelerated via diffusive shock acceleration (DSA) in weak shocks of Ms < 3.0. In the DSA theory, the particle momentum should be greater than a few times the momentum of thermal protons to cross the shock transition and participate in the Fermi acceleration process. In the equilibrium, the momentum of thermal electrons is much smaller than the momentum of thermal protons, so electrons need to be pre-accelerated before they can go through DSA. To investigate such electron injection process, we study the electron pre-acceleration in weak quasi-perpendicular shocks (Ms = 2.0 - 3.0) in an ICM plasma (kT = 8.6 keV, beta = 100) through 2D particle-in-cell simulations. It is known that in quasi-perpendicular shocks, a substantial fraction of electrons could be reflected upstream, gain energy via shock drift acceleration (SDA), and generate oblique waves via the electron firehose instability (EFI), leading the energization of electrons through wave-particle interactions. We find that such kinetic processes are effective only in supercritical shocks above a critical Mach number, $Ms{\ast}{\sim}2.3$. In addition, even in shocks with Ms > 2.3, energized electrons may not reach high energies to be injected to DSA, because the oblique EFI alone fails to generate long-wavelength waves. Our results should have implications for the origin and nature of radio relics.

• Journal of Electrochemical Science and Technology
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• v.13 no.3
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• pp.398-406
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• 2022

To satisfy the growing demand for high-performance batteries, diverse novel anode materials with high specific capacities have been developed to replace commercial graphite. Among them, cobalt hydroxides have received considerable attention as promising anode materials for lithium-ion batteries as they exhibit a high reversible capacity owing to the additional reaction of LiOH, followed by conversion reaction. In this study, we introduced graphene in the fabrication of Co(OH)₂-based anode materials to further improve electrochemical performance. The resultant Co(OH)₂/graphene composite exhibited a larger reversible capacity of ~1090 mAh g^-1, compared with ~705 mAh g^-1 for bare Co(OH)₂. Synchrotron-based analyses were conducted to explore the beneficial effects of graphene on the composite material. The experimental results demonstrate that introducing graphene into Co(OH)₂ facilitates both the conversion and reaction of the LiOH phase and provides additional lithium storage sites. In addition to insights into how the electrochemical performance of composite materials can be improved, this study also provides an effective strategy for designing composite materials.

• The Bulletin of The Korean Astronomical Society
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• v.46 no.2
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• pp.51.1-51.1
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• 2021

Merger shocks with M_s < ~ 3 - 4 have been detected in galaxy clusters through radio observations of synchrotron radiations emitted from cosmic-ray (CR) electrons. The CR electrons are believed to be produced by the so-called diffusive shock acceleration (DSA) at the merger shocks. To describe the acceleration of electrons, the injection into DSA has to be understood. Recent studies have showed that electrons could be energized through stochastic shock drift acceleration (SSDA), a mechanism mediated by multi-scale plasma waves at shock transition zone. However, such preacceleration process seems to be effective only at the supercritical shocks with M_s > ~ 2.3, implying that further studies should be done to explain radio relics with weaker shocks. In this talk, we present the results obtained by fully kinetic 2D particle-in-cell (PIC) simulations, which include pre-existing suprathermal electrons possibly ejected from active galactic nuclei (AGNs) or produced by previous episodes of turbulence/shocks. The simulations indicate that the pre-existing electrons enhance the upstream plasma waves in shocks with M_s < ~ 2.3. However, the wavelength of such waves is not long enough to scatter off suprathermal electrons and energize them to the injection momentum for DSA. Hence, we conclude that preexciting suprathermal electrons alone would not solve the problem of electron acceleration at radio relic shocks.

• The Bulletin of The Korean Astronomical Society
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• v.46 no.1
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• pp.36.2-36.2
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• 2021

In the outskirts of galaxy clusters, weak shocks with M_s < ~3 appear as radio relics where the synchrotron radiation is emitted from cosmic-ray (CR) electrons. To understand the production of CR electrons through the so-called diffusive shock acceleration (DSA), the electron injection into the DSA process at shocks in the hot intracluster medium (ICM) has to be described. However, the injection remains as an unsolved, outstanding problem. To explore this problem, 2D Particle-in-Cell (PIC) simulations were performed. In this talk, we present the electron preacceleration mechanism mediated by multi-scale plasma waves in the shock transition zone. In particular, we find that the electron preacceleration is effective only in the supercritical shocks, which have the sonic Mach number M_s > M_crit ≈ 2.3 in the high-beta (β~100) plasma of the ICM, because the Alfven ion cyclotron instability operates and hence multi-scale plasma waves are induced only in such supercritical shocks. Our findings will help to understand the nature of radio relics in galaxy clusters.

• Nuclear Engineering and Technology
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• v.56 no.1
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• pp.114-122
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• 2024

Irradiation-induced defects and the precipitates in the wrapper material of the Indian Fast Breeder Test Reactor (FBTR), SS 316 are analyzed using the synchrotron source-based Angle Dispersive X-Ray Diffraction (ADXRD) technique with X-rays of energy 17.185 keV (wavelength ~0.72146 Å). The differences and similarities in the high displacement damage samples as a function of dpa (displacement per atom) and dpa rate in the range of 2.9 × 10^-7- 9 × 10^-7 dpa/s are studied. Ferrite and M₂₃C₆ are commonly observed in the present set of high displacement damage 40-74 dpa SS 316 samples irradiated at temperatures in the range of 400-483 ℃. Also, the dislocation density has increased as a function of the irradiation dose. The X-ray diffraction peak profile parameters quantified such as peak shift and asymmetry show that the irradiation-induced defects are sensitive to the dpa rate-irradiation temperature combinations. The increase in yield strength as a function of displacement damage is also correlated to the dislocation density.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.7 no.3
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• pp.349-358
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• 1997

meta-Nitroaniline(mNA) crystals were grown from the supercooled melt for the first time. A seed was introduced into the purified mNA melt of 100 ml 0.1 K above the melting temperature ($T_m$ : $112.0^{\circ}C$) and crystal was grown at constant supercooling of 0.1 K. The melt was stirred . mechanically and the crystal was also rotated while the growth proceeds. mNA crystals of size up to $20{\times}15{\times}15 \textrm {mm}^3 and of very high perfection could be grown for the period of one day. The bottom half of the crystal faces are well-faceted and covered by {111} and {021} faces, while the faces of the top half are not well defined. The overall crystal morphology was characterized by the unidirectional growth along one of the polar axes. The absolute direction of preferred growth was determined to be [001] by the pyroelectric measurements. The perfection of the crystal was characterized by synchrotron X-ray topography and optical characterization was made by measuring second harmonic conversion efficiency.

• Progress in Superconductivity
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• v.14 no.2
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• pp.99-101
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• 2012

Fluorescent yield X-ray absorption fine structure (XAFS) spectroscopy is useful for analyzing local structure of specific elements in matrices. We developed an XAFS apparatus with a 100-pixel superconducting tunnel junction (STJ) detector array with a high sensitivity and a high resolution for light-element dopants in wide-gap semiconductors. An STJ detector has a pixel size of $100{\mu}m$ square, and an asymmetric layer structure of Nb(300 nm)-Al(70 nm)/AlOx/Al(70 nm)-Nb(50 nm). The 100-pixel STJ array has an effective area of $1mm^2$. The XAFS apparatus with the STJ array detector was installed in BL-11A of High Energy Accelerator Research Organization, Photon Factory (KEK PF). Fluorescent X-ray spectrum for boron nitride showed that the average energy resolution of the 100-pixels is 12 eV in full width half maximum for the N-K line, and The C-K and N-K lines are separated without peak tail overlap. We analyzed the N dopant atoms implanted into 4H-SiC substrates at a dose of 300 ppm in a 200 nm-thick surface layer. From a comparison between measured X-ray Absorption Near Edge Structure (XANES) spectra and ab initio FEFF calculations, it has been revealed that the N atoms substitute for the C site of the SiC lattice.

• Publications of The Korean Astronomical Society
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• v.27 no.4
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• pp.195-200
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• 2012

We show how the rotation emission from isolated interstellar Polycyclic Aromatic Hydrocarbons (PAHs) can explain the so-called anomalous microwave emission (AME). AME has been discovered in the last decade as microwave interstellar emission (10 to 70 GHz) that is in excess compared to the classical emission processes: thermal dust, free-free and synchrotron. The PAHs are the interstellar planar nano-carbons responsible for the near infrared emission bands in the 3 to 15 micron range. Theoretical studies show that under the physical conditions of the interstellar medium (radiation and density) the PAHs adopt supra-thermal rotation velocities, and consequently they are responsible for emission in the microwave range. The first results from the PLANCK mission unexpectedly showed that the AME is not only emitted by specific galactic interstellar clouds, but it is present throughout the galactic plane, and is particularly strong in the cold molecular gas. The comparison of theory and observations shows that the measured emission is fully consistent with rotation emission from interstellar PAHs. We draw the main lines of our PLANCK-AKARI collaborative program which intends to progress on this question by direct comparison of the near infrared (AKARI) and microwave (PLANCK) emissions of the galactic plane.

• Bulletin of the Korean Chemical Society
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• v.33 no.10
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• pp.3303-3310
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• 2012

Two single crystals of fully dehydrated partially $Li^+$-exchanged zeolite X were prepared by the exchange of Na-X, $Na_{92}Si_{100}Al_{92}O_{384}$ (Si/Al = 1.09), with $Li^+$ using aqueous 0.1 M $LiNO_3$ at 293 (crystal 1) and 333 K(crystal 2), followed by vacuum dehydration at 623 K and $1{\times}10^{-6}$ Torr for 2 days. Their structures were determined by single-crystal synchrotron X-ray diffraction techniques in the cubic space group $Fd{\overline{3}}$ at 100(1) K. Their structures were refined using all intensities to the final error indices (using the 1281 and 883 reflections for which ($F_o$ > $4{\sigma}(F_o)$) $R_1/R_2$ = 0.075/0.244 and 0.074/0.223 for crystals 1 and 2, respectively. Their compositions are seen to be ${\mid}Li_{86}Na_6{\mid}[Si_{100}Al_{92}O_{384}]$-FAU and ${\mid}Li_{87}Na_5{\mid}[Si_{100}Al_{92}O_{384}]$-FAU, respectively. In crystal 1, 17 $Li^+$ ions per unit cell are at site I', 15 another site I', 30 at site II, and the remaining 16 at site III; 2 $Na^+$ ions are at site II and the remaining 4 at site III'. In crystal 2, 32 and 30 $Li^+$ ions per unit cell fill sites I' and II, respectively, and the remaining 25 at site III'; 2 and 3 $Na^+$ ions are found at sites II and III', respectively. The extent of $Li^+$ exchange increases slightly with increasing ion exchange temperature from 93% to 95%.