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Follicular Stimulation and Laparoscopic Ovum Pick-up (LOPU) in Repeatedly Superovulated Korean Black Goats (반복적인 과배란 처치 경험이 있는 한국흑염소에서 난포 자극 및 복강경을 이용한 난포란 채취(LOPU))

  • Lee, Yong-Boum;Lee, Doo-Soo;Cho, Sang-Cheol;Shin, Sang Tae
    • Journal of Embryo Transfer
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    • v.29 no.1
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    • pp.35-41
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    • 2014
  • Laparoscopic ovum pick-up (LOPU) is a convenient method for collecting oocytes in small ruminants. LOPU has the advantage of being a less invasive means of oocyte collection, thereby allowing for a repeated usage of the oocyte donor animals. A total of 25 Korean black goats were used in the winter season (December to February) and LOPU was applied to the goats which had been treated for superovulation more than two times during the last twelve months. Estrus was synchronized with an intravaginal insert containing 0.3 g progesterone for 10 to 12 days. Ovaries were hyperstimulated with eCG 1,000 IU oneshot, FSH with eCG (50 mg / 1,000 IU; 70 mg / 500 IU; 70 mg / 1,000 IU) oneshot or FSH multiple-shot with eCG oneshot ($20mg{\times}6/300IU$) given intramuscularly 72 h prior to LOPU. For these groups, the number of follicles (mean ${\pm}$ SEM) observed which developed to larger than 2 mm in diameter were $1.6{\pm}2.5$, $4.3{\pm}3.1$, $5.5{\pm}4.2$, $6.6{\pm}2.1$ and $8.8{\pm}7.8$, respectively. Oocytes were aspirated by using OPU needles and a vacuum pump. The overall oocyte retrieval rates were 41.4%. Oocytes were matured in TCM-199 supplemented with 10% (w/v) bovine serum albumin + $10{\mu}g/ml$ FSH + $1{\mu}g/ml$ $17{\beta}$-estradiol for 27 h at $39^{\circ}C$ in 5% $CO_2$ in air. Oocytes were parthenogenetically activated by ionomycin combined with 6-diethylaminopurine (6-DMAP). Total oocyte maturation and cleavage rate were 67.3% and 78.8%, respectively. In summary, LOPU is a useful oocyte collection method in Korean black goats that can provide immature oocytes for transgenesis or nuclear transfer.

Gated Conductivity Imaging using KHU Mark2 EIT System with Nano-web Fabric Electrode Interface (나노웹 섬유형 전극 인터페이스와 KHU Mark2 EIT 시스템을 이용한 생체신호 동기 도전율 영상법)

  • Kim, Tae-Eui;Kim, Hyun-Ji;Wi, Hun;Oh, Tong-In;Woo, Eung-Je
    • Journal of Biomedical Engineering Research
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    • v.33 no.1
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    • pp.39-46
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    • 2012
  • Electrical impedance tomography(EIT) can produce functional images with conductivity distributions associated with physiological events such as cardiac and respiratory cycles. EIT has been proposed as a clinical imaging tool for the detection of stroke and breast cancer, pulmonary function monitoring, cardiac imaging and other clinical applications. However EIT still suffers from technical challenges such as the electrode interface, hardware limitations, lack of animal or human trials, and interpretation of conductivity variations in reconstructed images. We improved the KHU Mark2 EIT system by introducing an EIT electrode interface consisting of nano-web fabric electrodes and by adding a synchronized biosignal measurement system for gated conductivity imaging. ECG and respiration signals are collected to analyze the relationship between the changes in conductivity images and cardiac activity or respiration. The biosignal measurement system provides a trigger to the EIT system to commence imaging and the EIT system produces an output trigger. This EIT acquisition time trigger signal will also allow us to operate the EIT system synchronously with other clinical devices. This type of biosignal gated conductivity imaging enables capture of fast cardiac events and may also improve images and the signal-to-noise ratio (SNR) by using signal averaging methods at the same point in cardiac or respiration cycles. As an example we monitored the beat by beat cardiac-related change of conductivity in the EIT images obtained at a common state over multiple respiration cycles. We showed that the gated conductivity imaging method reveals cardiac perfusion changes in the heart region of the EIT images on a canine animal model. These changes appear to have the expected timing relationship to the ECG and ventilator settings that were used to control respiration. As EIT is radiation free and displays high timing resolution its ability to reveal perfusion changes may be of use in intensive care units for continuous monitoring of cardiopulmonary function.

Non-gaseous Plasma Immersion Ion Implantation and Its Applications

  • Han, Seung-Hee;Kim, En-Kyeom;Park, Won-Woong;Moon, Sun-Woo;Kim, Kyung-Hun;Kim, Sung-Min
    • Proceedings of the Korean Vacuum Society Conference
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    • 2012.08a
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    • pp.151-151
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    • 2012
  • A new plasma process, i.e., the combination of PIII&D and HIPIMS, was developed to implant non-gaseous ions into materials surface. HIPIMS is a special mode of operation of pulsed-DC magnetron sputtering, in which high pulsed DC power exceeding ~1 kW/$cm^2$ of its peak power density is applied to the magnetron sputtering target while the average power density remains manageable to the cooling capacity of the equipment by using a very small duty ratio of operation. Due to the high peak power density applied to the sputtering target, a large fraction of sputtered atoms is ionized. If the negative high voltage pulse applied to the sample stage in PIII&D system is synchronized with the pulsed plasma of sputtered target material by HIPIMS operation, the implantation of non-gaseous ions can be successfully accomplished. The new process has great advantage that thin film deposition and non-gaseous ion implantation along with in-situ film modification can be achieved in a single plasma chamber. Even broader application areas of PIII&D technology are believed to be envisaged by this newly developed process. In one application of non-gaseous plasma immersion ion implantation, Ge ions were implanted into SiO2 thin film at 60 keV to form Ge quantum dots embedded in SiO2 dielectric material. The crystalline Ge quantum dots were shown to be 5~10 nm in size and well dispersed in SiO2 matrix. In another application, Ag ions were implanted into SS-304 substrate to endow the anti-microbial property of the surface. Yet another bio-application was Mg ion implantation into Ti to improve its osteointegration property for bone implants. Catalyst is another promising application field of nongaseous plasma immersion ion implantation because ion implantation results in atomically dispersed catalytic agents with high surface to volume ratio. Pt ions were implanted into the surface of Al2O3 catalytic supporter and its H2 generation property was measured for DME reforming catalyst. In this talk, a newly developed, non-gaseous plasma immersion ion implantation technique and its applications would be shown and discussed.

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Biological Control of Polyphagotarsonenmus latus(Acari: Tasonemidae) by Amblyseius cucumeris(Acari: Phytoseiidae) (오이이리응애(Amblyseius cucumeris)를 이용한 차먼지응애(Polyphagotarsonemus latus) 생물적 방제)

  • La, Seung-Yong;Paik, Chae-Hoon;Lee, Geon-Hwi;Kim, Doo-Ho
    • Korean journal of applied entomology
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    • v.48 no.3
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    • pp.347-354
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    • 2009
  • This study was conducted on the biological control effects of the natural enemy, Amblyseius cucumeris, against Polyphagotarsonemus latus. The broad mite moved to a new habitat after the carrying capacity of the preferred habitat on the upper leaf has been reached. Population dynamics between predator, Amblyseius cucumeris, and prey, P. latus, were quite well synchronized since the predator followed the movement of the prey. In predation test by Amblyseius cucumeris on P. latus, A. cucumeris fed on 8.8, 15.2, 27.0 and 30.3 individuals/day under the temperatures of 15, 20, 25, and $30^{\circ}C$, respectively. The A. cucumeris larva, protonymph, deutonymph, adult male, and adult female fed on 0.5, 1.6, 5.2, 3.8, and 27 individuals of adult P. latus per day, respectively, under $25^{\circ}C$ condition. The functional response curve of adult P. latus to the densities of A. cucumeris showed Holling's Type II curve. The consumption of prey by A. cucumeris increased with the prey density increase, but the consumption rate decreased. The critical ratio of predator vs. prey effectively to suppress (98% over) the population of P. latus by releasing A. cucumeris was 1:40 on red pepper.

지지적 간호중재가 두경부암 수술환자의 불안, 우울에 미치는 효과

  • Seok, Jeong-Hui;Gang, Eun-Sil
    • Korean Journal of Hospice Care
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    • v.3 no.2
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    • pp.19-33
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    • 2003
  • Cancer causes many crises to cancer patients imcluding physical dysfunction and emotional changes such as anxiety, depression as well as a threat of life, fear of death. As it develops, cancer makes people feel powerlessness due to the losses of their own positions, roles and independence. Although occupying a little proportion among all types of cancer, head and neck cancer may cause a wide range of physical transformation by surgical operation, damage to active functions such as eating and speaking, provoke anxiety and depression after its operation, influencing the quality life of head and neck cancer patients. Thus nursing intervention should be developed to provide supportive nursing for head and neck cancer patients and play roles as competent supporters. This study is a nonequivalent, control group, pretest-posttest, non-synchronized quasi-experimental research design to determine, how nursing intervention has effects on anxiety, depressing of head and neck cancer and operated. They were divided into experimental and comparison groups, each consisting of 20 members. The data were collected during the period from December 1, 1999 to April 11, 2000. Tools of the study included the protocol of supportive nursing intervention which was developed by researcher with reference to a literal review and esperts' advice. The measurement tool of anxiety was consisting of totaled 20 question items which was prepared by Spielberger and translated by Kim et al., the device of depression measurement consisting of total 20 question items which was the output of Song's translation the device of depression self-evaluation from Zung. Data were analyzed using the SPSS/PC 9.0 program. The homogeneity of the subjects were tested using x2-test and t-test. 5 hypoteses were tested using t-test. The results of the study can be summarized as follows. 1.The first hypothesis that the experimental group receiving supportive nursing intervention shows a little anxiety than the control group not receiving supportive nursing intervention was supported(t=3.817, P=.000). 2.The second hypothesis that the experimental group receiving supportive nursing intervention shows a little depression than the control group not receiving supportive nursing intervention was supported(t=8.089, P=.000). Consequently, supportive nursing intervention was found an effective nursing intervention strategy to reduce anxiety and depression of head and neck cancer patients. Providing supportive nursing intervention in nursing practice can enhance the quality of life of those cancer patients.

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An Analysis on the Change of Usage Behavior for Bundle Services in Korean Telecommunication Market (방송통신 결합상품서비스 이용행태 변화 분석 연구)

  • Yu, Jieun;Lee, Seong-Jun;Cho, Chanwoo
    • The Journal of the Korea Contents Association
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    • v.20 no.11
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    • pp.204-215
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    • 2020
  • We analyze changes of consumption behavior for bundle services for the latest three years focusing on comparing the detailed service types and service providers to understand competition structure in the market. Our major findings and implications are as follows. First, the mobile telecommunication service is certainly deemed to be a major selection criteria for bundling service provider, therefore the leading company in the mobile telecommunication has become more significant market power. Second, IPTV has an important influence for slection of bundling service provider, lately in some cases, rather than broadband Internet. Third, the individual services affecting the selection of bundling service providers clearly showed differentiation among three operators as a mobile telecommunication service of SK Group, a broadband Internet of KT, and an IPTV of LGU+. In addition, the importance of individual services in securing subscribers has been increased as the preference factors to switch a service provider were synchronized with the decision of major bundling service provider. Finally, although it is difficult to find customers' lock-In due to the latest change of their intentions to switch a provider, we find it is continuously necessary to monitor customers' lock-in as there are more long-term customers with re-contracts. Our results provide the policy implications based on the change of competition structure and usage behavior in the bundle market which is not evaluated the competiton situation.

Dynamic O-D Trip estimation Using Real-time Traffic Data in congestion (혼잡 교통류 특성을 반영한 동적 O-D 통행량 예측 모형 개발)

  • Kim Yong-Hoon;Lee Seung-Jae
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.5 no.1 s.9
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    • pp.1-12
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    • 2006
  • In order to estimate a dynamic origin and destination demand between on and off-ramps in the freeways, a traffic flow theory can be used to calculate a link distribution proportion of traffics moving between them. We have developed a dynamic traffic estimation model based on the three-phase traffic theory (Kerner, 2004), which explains the complexity of traffic phenomena based on phase transitions among free-flow, synchronized flow and moving jam phases, and on their complex nonlinear spatiotemporal features. The developed model explains and estimates traffic congestion in terms of speed breakdown, phase transition and queue propagation. We have estimated the link, on and off-ramp volumes at every time interval by using traffic data collected from vehicle detection systems in Korea freeway sections. The analyzed results show that the developed model describes traffic flows adequately.

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Implementation of a Web-based Virtual Educational System for Java Language Using Java Web Player (자바 웹플레이어를 이용한 웹기반 자바언어 가상교육시스템의 구현)

  • Kim, Dongsik;Moon, Ilhyun;Choi, Kwansun;Jeon, Changwan;Lee, Sunheum
    • The Journal of Korean Association of Computer Education
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    • v.11 no.1
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    • pp.57-64
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    • 2008
  • This paper presents a web-based virtual educational system for Java language, which consists of a management system named Java Web Player (JWP) and creative multimedia contents for the lectures of Java language. The JWP is a Java application program free from security problems by the Java Web Start technologies that supports an integrated learning environment including three important learning procedures: Java concept learning process, programming practice process and assessment process. On-line voice presentation and its related texts together with moving images are synchronized for efficiently conveying creative contents to learners. Furthermore, a simple and useful compiler is included in the JWP for providing user-friendly language practice environment enabling such as coding, editing, executing, and debugging Java source files on the Web. Finally, simple multiple choices are given suddenly to the learners while they are studying through the JWP and the test results are displayed on the message box. In order to show the validity of the proposed virtual educational system we analysed and assessed the learners' academic performance on the five quizzes for one semester.

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The Effect of a Video Exercise Program on Cancer-related Fatigue, Physical Function and Emotional Status in Patients with Cancer during Chemotherapy (동영상 운동프로그램이 항암 화학요법을 받는 암환자의 피로와 신체기능 및 정서상태에 미치는 효과)

  • Cho, Mee Young;Park, Ji Young;Lee, Chung Eun;Song, Su Kyung;Lee, Sun Hi;Byun, Eun Sung;Kim, Ji Youn;Park, Ok Sun;Kim, Soon Ho;Kang, Young Lynn;Han, Soo Young;Lee, Hyang Kyu;Choi, Hye Jin
    • Journal of Korean Clinical Nursing Research
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    • v.18 no.3
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    • pp.368-380
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    • 2012
  • Purpose: The purpose of this study was to evaluate the effects of a home-based video exercise program on cancer-related fatigue, physiological and psychological status in patients with colon and rectal cancers undergoing chemotherapy. Methods: The study design was a non-equivalent control group non-synchronized design. Data were collected from patients with colo-rectal cancers in Yonsei cancer center from July 5th to October 31st in 2011. There were 40 participants; 20 in the experimental group and 20 in the control group. The structured questionnaire was used to measure fatigue, physical function and emotional status. Data were analyzed using SPSS 18.0 and a chi-squre test, Fisher's exact test, Mann-Whitney U test and Wilcoxon signed- rank test were conducted to examine the homogeneity and the research hypotheses. Results: There was a statistically significant difference in White Blood Cell count in the experimental group compared with that of the control group. The exercise group showed a slight decrease of White Blood Cell count compared with that of the control group after 4 week program (z=-2.935, p=.003). However, there were no significant differences in fatigue, physiological and psychological status between the two groups. Conclusion: In this study, the developed video exercise program was effective in markedly slightly decreasing White Blood Cell count in patients with colo-rectal cancers undergoing chemotherapy. Therefore, utilizing the video exercise program can be an useful method to promote health among patients with cancer in clinical practice.

Estimation of Fractional Frequency Offset for the Next-Generation Digital Broadcasting System (차세대 디지털 방송시스템을 위한 소수배 주파수 오프셋 추정)

  • Kim, Ho Jae;Kang, In-Woong;Seo, Jae Hyun;Kim, Heung Mook;Kim, Hyoung-Nam
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.41 no.11
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    • pp.1364-1373
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    • 2016
  • Ultra High Definition Television (UHDTV) has attracted much attention as one of next generation broadcasting services. For the commercialization of UHD broadcasting service, standardization groups including the DVB (Digital Video Broadcasting) and the ATSC (Advanced Television Systems Committee) have decided to adopt the Orthogonal Frequency Division Multiplexing (OFDM) for signal transmission. However, when the carrier frequency is not properly synchronized at the receiver, inter-symbol interference (ISI) and inter-carrier interference (ICI) may occur. In order to avoid performance degradation resulting from ISI or ICI, receivers should synchronize the carrier frequency by using preambles and pilot symbols. However, there only few published literature dealing with the frequency offset estimation methods regarding the next generation terrestrial broadcasting. In this respect, this paper proposes a method to estimate timing and fractional frequency offset for the ATSC 3.0 system by using a preamble bootstrap symbol. The proposed detector can detect the fractional frequency offset by adding a complex conjugate product on the conventional estimator where only timing offset can be estimated.