• 수산해양기술연구
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• 제50권3호
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• pp.310-317
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• 2014

This study was conducted to investigate the attractant substances of different solvent fractions of mackerel and tuna viscera. Mackerel and tuna viscera were extracted with methanol and the resultant were fractionated with n-hexane, chloroform, ethyl acetate, n-butanol and water. The chemical compounds such as free amino acid, fatty acid, nucleotide related compounds contents were estimated for mackerel, tuna viscera and various fractions. These fractions were also subjected to attracting experiments in water tank to estimate attracting effects. The aqueous fractions of mackerel and tuna viscera showed the highest attraction effect on swimming crab. The major chemical compounds of the aqueous fractions were histidine, taurine, cysteine, glutamic acid, inosine monophosphate (IMP) and inosine (HxR) for mackerel and arginine, glutamic acid, aspartic aid, alanine, IMP and hypoxanthin (Hx). Results indicated that higher polarity compounds, such as amino acid and nucleotide related compounds have higher attraction activities than nonpolar substances such as neutral lipid.

• 수산해양기술연구
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• 제43권4호
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• pp.301-309
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• 2007

In order to develop the artificial bait of trap for swimming crabs, the attracting effect was analyzed in the duration time of attracting effect, preference and shape recognition to the bait in the water tank and field experiments. According to the result which investigated the mean entrapped number of swimming crabs into the trap for each bait(mackerel, sardine and anchovy) in water tank experiments, the mean entrapped number was found to be higher in the trap with mackerel(6.9 ind.; 23.0%) than in other traps with sardine(5.2 ind.; 17.3%) and anchovy(3.4 ind.; 11.3%). In addition, the mean entrapped ratios of swimming crabs into the traps for 3 kinds of baits were estimated to be over 80% all in 5 hours after soaked in the water tank. For preference of swimming crabs to 3 kinds of baits, the trap with mackerel(6.9 ind.; 23.0%) was evaluated to be higher than the traps with sardine(2.8 ind.; 9.3%) and anchovy(0.2 ind.; 0.7%). In the field experiments, the mean entrapped number of swimming crabs into the trap with mackerel and sardine were found to be similar as 120 and 118 ind., but the trap with anchovy was only 85 ind.. According to the result which investigated the shape regnition of swimming crabs to the bait used mackerel, divided into 8 pieces, 16 pieces and grinded mass in water tank experiments, the mean entrapped number of swimming crabs into the traps that includes the mackerel divided into several pieces was significantly decreased. Moreover, by the presence of skin of mackerel for bait, there was a great difference in the entrapped number of swimming crabs. Therefore, it was thought that the swimming crab was attracted to the bait and entrapped into the trap would indicate that not only the chemical stimuli such as the attactant, but also the visual stimuli such as the shape recognition to the bait might be closely related.

• 생명과학회지
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• 제16권4호
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• pp.672-675
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• 2006

In order to determine the morphological variations of the Japanese swimming crab, Charybdis japonica, we conducted morphological analysis of five populations that are relatively abundant in Korea and Japan. Abdominal sternite for individuals of the Tean population showed significantly short when compared to those of other populations. Principal component analysis demonstrated that morphological differentiation was due to differences (A/Q, B/M, D/P, F/O, J/K), and effectively discriminated each others. The first two components accounted for 40.9% and 60.7% of the variation, respectively. The dendrogram showed three distinct groups, Tokyo population, Taean population, and the other populations.

• 수산해양기술연구
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• 제47권4호
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• pp.316-326
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• 2011

In this study, the entrapped number is investigated on the UV light with different illuminance to fluorescent bait cage for swimming crab in order to find the appropriate illuminance which has the best attraction effect of fluorescent bait cage for pots. In addition, preference to the light, arrival time and residence time at light area are compared and analyzed to fluorescent bait cage and non-fluorescent bait cage for American lobster at the UV light and ordinary light according to the illuminance condition. Pot with red non-fluorescent bait cage at the no lighting (<0.01lux), pot with blue fluorescent bait cage at the 20W UV lighting (0.16lux) and pot with blue fluorescent bait cage at the 30W UV lighting (0.22lux) were soaked for 6 hours and the entrapped number of swimming crab was examined. The mean entrapped number of swimming crab in pot with red non-fluorescent bait cage at the no lighting (<0.01lux) was 1.0, but the mean entrapped number of swimming crab in pot with blue fluorescent bait cages at the 20W UV lighting (0.16lux) and 30W UV lighting (0.22lux) were 1.4 and 0.4, respectively (P<0.05). The rate of preference to the blue fluorescent bait cage at the UV lighting shows 1.6-4.8 times higher than that of preference to the red non-fluorescent bait cage at the ordinary lighting. In addition, The rate of preference to the blue fluorescent bait cage at the UV lighting is higher when the illuminance of ordinary light is same as or is lower than that of UV light (P<0.05). However, the preference to the light depending on gender shows no significant difference (P>0.05). The arrival time to UV light area of lobster is shown as 1.2-2.4 times faster than that to ordinary light area. Generally, it is shown that arrival time to UV light area is faster than the arrival time to ordinary light area when the illuminance of ordinary light is the same as or lower than that of UV light (P<0.05). However, arrival time to the light area depending on gender shows no significant difference (P>0.05). The residence time at UV light area of lobster is 1.2-1.7 times longer than that at ordinary light area. The residence time depending on different illuminance of ordinary light and genders showed no significant difference (P>0.05).

• 한국수산과학회지
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• 제37권3호
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• pp.186-191
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• 2004

The effects of temperature on the growth of larvae of wrinkled swimming crab (Liocarcinus corrugatus) under controlled laboratory conditions of 15, 20 and $24^{\circ}C$ were investigated (33.5 psu: 12L: 12B). Each specimen was maintained individually, the instar size was determined from exuvia, and the survival and intermolt period of each instar were recorded. There were no significant differences (P>0.05) in the survival rates at 15, 20, and $24^{\circ}C.$ Comparison of survival over the first 27 days between the three temperature regimes showed significant differences (P<0.001). The intermolt period increased with the instar number, and was greatly extended at the lowest temperature. The molt increment varied little with temperature, and had a mean value of $21.35\%$ over the whole study. Temperature had little effect on survival and molt increment.

• 수산해양기술연구
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• 제44권4호
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• pp.282-293
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• 2008

In order to develop the substitutive materials for natural baits of swimming crab pots, the attracting effects of swimming crab such as the preference of baits which were made of the by-products of marine and stock raising through the water tank experiments and fishing experiments. On the investigation of mean entrapped catch number to the pot by the baits after putting the 4 kinds of baits, mackerel(M), mackerel with grinded mackerel s internals($MM_I$), mackerel with tuna s internals$MM_I$) and makerel with grinded krill(MK) each in one pot by turns, $MM_I$ and MK were entrapped mean 3.9(13.0%) and they were a little more comparing to M, and $MT_I$ is least with mean 2.1(7.0%)(F=12.913, P < 0.05). Otherwise, on the preference investigation of swimming crabs by the baits after putting the 4 kinds of baits in the 4 pots each, M was entrapped mean 3.0(10%), but $MM_I$, $MT_I$ and MK were mean 1.2(4.0%), 1.0(3.3%) and 1.5(5.0%) each and they were only 30-50% of M(F=13.398, P < 0.05). On the preference investigation of swimming crabs by the 5 kinds of baits, mackerel(M), and krill(K), manila clam($M_C$), pig s fat($P_F$) and chicken s head($C_H$) which were used in substitutive baits, M was entrapped mean 3.2(10.7%), but K was about 50% of catch of M with mean 1.6(5.3%), and $M_C$, $P_F$ and $C_H$ were very few with mean 0.1-0.2(0.3-0.7%)(F=89.186, P < 0.05). On the preference investigation of swimming crabs by the pots which were put each the 3 kinds of baits, original krill(K), grinded krill with gluten and soybean oil cake($K_GGS$) and grinded krill with gluten, soybean oil cake and glycine($K_GGSG_L$) in the blue fluorescent hexahedral plastic bait cages(BF), and which were put the mackerel(M) in the non-fluorescent hexahedral red plastic bait cage($RF_N$), it was entrapped mean 3.0(10.0%) in the pot which was put the mackerel in the $RF_N$, and the same level in the pots which were put the K and $K_GGSG_L$ in the BF, but it was mean 2.0(6.7%) in the pots which was put the $K_GGS$ in BF and it was decreased by 30% of catch comparing to $RF_N$(F=3.750, P < 0.05). On the preference investigation of swimming crab by the pots which was put grinded tuna with gluten, soybean oil cake and glycine($T_IGSG_L$) in the blue fluorescent hexahedral plastic bait cage(BF), and which was put mackerel(M) in the nonfluorescent hexahedral red plastic bait cage($RF_N$), it was entrapped mean 3.3(11.0%) in the pot which was put mackerel in $RF_N$, and mean 2.7(9.0%) in the pot which was put $T_IGSG_L$ in BF and it was about 15% less comparing to use bait M(t=1.387, P < 0.05). As a results of fishing experiments, a plan for enhancing catching efficiency of $T_IGSG_L$ will be required because catching efficiency of $T_IGSG_L$, alternative bait, was half of fish catching efficiency of natural bait using mackerel. Fishing experiments were conducted 3 times using reinforced substitutive artificial bait that is reinforced attractive effect of $T_IGSG_L$ and composed of tuna intestine, grinded mackerel, gluten, soybean cake, glycine and alanine($T_IM_GGSG_LA$). Catching efficiency of $T_IM_GGSG_LA$ was about 80% of that of natural bait made of mackerel.

• 한국수산과학회지
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• 제35권2호
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• pp.122-129
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• 2002

민꽃게를 시료로 하여 바람직한 갑각류 향기성분의 전구물질부가 되는 character impact compounds의 검색 및 게 자숙향기의 발현에 대하여 검토하였다. Methanol이 냄새성분의 전구물질 추출에 가장 적합한 것으로 나타났고, 추출횟수는 3회 정도로 완전추출이 가능했으며, 이때 methanol의 농도는 $80\%$일 때가 냄새의 강도가 가장 강한 것으로 나타났다. 시료 게의 냄새성분의 전구물질부를 구명하기 위해 투석 및 이온 교환수지 방법 등을 이용해 냄새성분 전구물질부를 분획하고 관능검사한 결과, 게 자숙취의 전구물질은 수용성이고 투석성의 저분자 성분으로, 산성 및 양성성분의 물질을 주체로 하여 여러 가지 성분이 동시에 가열될 때 발생하는 것이라고 생각되었다 게육 특유의 자숙취를 발현시킬 목적으로, 민꽃게 육의 $80\%$ methanol 추출성분의 조성에 근거한 합성 게육 엑스분의 조제하여 100$^{\circ}C$C에서 30분간 열처리하여 발생하는 냄새를 천연 게육의 자숙취와 비교하여 볼 때, 냄새의 조화성 면에서는 다소 떨어지나 양시료 사이에는 거의 차이가 없었다. 한편, 천연 및 합성 게육의 자숙취를 GC로 분석하여 chromatogram의 pattern을 비교한 결과 저·중비점 물질의 경우 양자가 서로 유사한 것으로 나타났다. 게 자숙취의 발현에는 염기성아미노산을 비롯한 아미노산류의 역할이 컸으며, ribose와 염기성아미노산 간에 일어나는 Maillard 반응에 의해 생성되는 휘발성화합물, 그리고 4급 암모니움염기가 민꽃게 자숙취의 주요 요소가 될 것으로 추정되었다.

• 수산해양기술연구
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• 제44권1호
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• pp.20-30
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• 2008

In order to evaluate the biodegradability of bait used in the pot for swimming crab, water tank experiments were conducted. Mackerel is the most commonly used natural form of bait to catch the swimming crabs, and therefore was used in this experiment for the biodegradability according to the manufacturing process of the bait. From the biodegradability test on chemical oxygen demand(COD), total nitrogen(T - N), total phosphorus(T - P), ammoniac nitrogen, nitrite nitrogen and nitrate nitrogen per unit weight of the bait based on the production rate and the accumulated amount of pollutants, it was concluded that the smaller the size of the mackerel pieces, the higher the production rate and accumulated amount of organic matter and nutrients which was unfavorable to water pollution. The amount of pollutants released from the intestine of the tuna was similar with that from the whole mackerel. For the operation period of 111 days, the accumulated concentrations of tested pollutants from the tuna which were 67.3 mgCOD/g d, 86.4 mgT N/g d, 3.1 mgT - P/g d, were almost half comparing with those from the mackerel which were 65.7 - 94.4 mgCOD/g d, 83.8 - 109.4 mgT - N/g d, 3.1 - 5.2 mgT - P/g d. The amount of pollutants released from the intestine of the tuna was slightly less than that from the mackerel that was cut into 8 pieces. but more than that from the mackerel which was not cut into pieces. Therefore, it can be concluded that the key factor in determining water pollution potential is not the kind of bait, but the processing or preparation method used.

• 생명과학회지
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• 제23권9호
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• pp.1088-1095
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• 2013

꽃게(Portunus trituberculatus)는 세계적으로 넓게 분포하는 갑각류로 모래나 돌멩이가 있는 해저에 서식한다. 본 연구는 서해의 4개 지점(영광, 태안, 소래, 연평도)에서 채집된 P. trituberculatus 281 개체에 대해 10 종류 microsatellite 좌위의 유전적 다형성을 조사하였다. 좌위당 대립유전자 수는 50-129개로 평균 69.5개였으며, 관측 및 예상 이형접합도는 각각 0.111-1.000 및 0.609-0.979 범위에 있었다. 좌위별 근친계수((Fis)는 -0.0207에서 0.8175 범위였다. 유전적 분화도(Fst)는 0.05보다 낮게 나타났는데, 이것은 4 꽃게 간의 유전적 분화(genetic differentiation)가 매우 낮은 이루어진 것으로 추정하게 한다. UPGMA을 이용한 계통도 작성에서도 4 그룹 간의 유전적 거리는 매우 가깝다는 결과를 얻을 수 있었다. 매우 높은 다형성과 집단간의 낮은 유전적 분화는 서해안의 꽃게 집단은 활발한 유전적 흐름(gene flow)이 일어나며, 그룹간 지리적 경계가 없음을 제시한다

• 수산해양기술연구
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• 제59권1호
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• pp.19-27
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• 2023

The interest in greenhouse gases (GHG) emitted from all industries is emerging as a very important issue worldwide. This is affecting not only the global warming, but also the environmentally friendly competitiveness of the industry. The fisheries sector is increasingly interested in greenhouse gas emissions also due to the Paris Climate Agreement in 2015. Korean industry and government are also making a number of effort to reduce greenhouse gas emissions so far, but the effort to reduce GHG in the fishery sector is insufficient compared to other fields. Especially, the investigation on the GHG emissions from Korean fisheries did not carry out extensively. The studies on GHG emissions from Korean fishery are most likely dealt with the GHG emissions by fishery classification so far. However, the forthcoming research related to GHG emissions from fisheries is needed to evaluate the GHG emission level by species to prepare the adoption of Environmental labels and declarations (ISO 14020). The purpose of this research is to investigate which degree of GHG emitted to produce the species (swimming crab and snow crab) from various fisheries. Here, we calculated the GHG emission to produce the species from the fisheries using the life cycle assessment (LCA) method. The system boundary and input parameters for each process level are defined for LCA analysis. The fuel use coefficients of the fisheries for the species are also calculated according to the fuel type. The GHG emissions from sea activities by the fisheries will be dealt with. Furthermore, the GHG emissions for producing the unit weight species and annual production are calculated by fishery classification. The results will be helpful to establish the carbon footprint of seafood in Korea.