• 한국환경교육학회지:환경교육
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• 제20권4호
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• pp.1-11
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• 2007

The purpose of this study is to develope energy eco-audit evaluation scheme for the sustainable energy management at schools in accordance with education for sustainable development. Energy eco-audit evaluation scheme was developed through critical review of preexisting evaluation methods, consensus searching for the process from field. The results of this study are as follows. The school energy eco-audit evaluation scheme has three criteria: data collection, energy program, and environmental review. First, criteria of data collection includes general affairs, school building construction, and energy use. Second, criteria of energy program stresses school administration system such as the democratic decision-making process and structures. Third, criteria of environmental review includes reporting process and preparing teaching/learning materials for sustainable energy management. In conclusion, school energy eco-audit evaluation scheme could find a new way to achieve extended effect for sustainable energy management in school. It also could continue to seek opportunities to raise their awareness of energy issue and environment. School committee and whole school are involved to continue to implement present action plan and prepare updated plan in order to reduce environmental impact in school. By doing so, it would be possible to play important role in both school administration and education for sustainable development.

• 한국태양에너지학회 논문집
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• 제30권4호
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• pp.63-70
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• 2010

This study introduce a architectural design process by the changes of sustainable design guidelines in public project. First of all, this study examines existing integrated design process studies performed by Rhee & U.B.C. Also, this study examines sustainable guidelines which demanded by public building projects. Then, this study introduces sustainable design process using existing sustainable design process, and shows that how to operate and proceed integrated design to satisfy the sustainable guidelines in practice. The results of this study are as follows. (1) Since 2007, sustainable design guidelines of bidding projects have been highlighted. (2) In public buildings, energy saving is very important issue. Especially energy certification demands 1st degree(under $300kWh/m^2{\cdot}yr$). (3) Inp ractice, integrated design team performed sustainable design using the sustainable design process to improve sustainable in public buildings.

• KIEAE Journal
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• 제10권5호
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• pp.3-12
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• 2010

Sustainable design is getting to be controversial issue in all industries over the world particularly, in architecture as the amount of energy usage in architecture occupies 40%. Therefore, it is essential to make the standard for the sustainable design. In order to construct the sustainable design, firstly it should be considered that sustainable design elements based on natural resources to increase building energy efficiency is established and classified. The method of sustainable design divides into passive design and active design. Passive design method should be examined with active one simultaneously for more efficient usage of energy. Next, the study is followed how the sustainable design elements is adopted in buildings through the comparison of cases study of domestic and oversea. The result of case study shows similar adoption of sustainable design elements between oversea and domestic. However, the difference is shown in the building orientation and shape and the window size and position in Solar energy as well as high performance structure in Heat energy. These elements are the most significant elements in order to reduce energy load. In oversea, sustainable design is generated by architects, a client, and consultants based on the close cooperation in the beginning of early design phase before deciding building shape and envelope while in the domestic field adoption for sustainable design is conducted after deciding building shape and material. In order to design sustainable architecture more study is necessary in early stage for Zero Carbon and reducing building energy load through relation with specialists, a client and architects.

• 한국환경교육학회지:환경교육
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• 제19권3호
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• pp.102-113
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• 2006

The purpose of this study is to devise proper plans for sustainable energy management in schools through applying school eco-audit to energy field. This study is performed by literature review and the analysis of case study. This study analyzes the energy-saving model school according to the three cores of eco-audit such as school administration system, teaching-learning method, and ecological policy, and the result of the study is as followings. First, according to the analysis of school administration system for energy education programs, the participation of all the school staff is very low, and the democratic decision-making system is unsatisfactory. Second, according to the analysis of teaching-learning method, the program is mainly managed by teacher-oriented method not by learner-oriented one. The effect on the education of energy-saving program is a little improved in cognitive area, but is insufficient in affective area. Third, in the aspect of the ecological policy, the practical energy-saving effect in schools is unsatisfactory, and shows the difficulty of continuing application of the program. Consequently, it is desirable to integrate the energy-saving program with the eco-audit for sustainable energy management in schools. Implementation of the energy eco-audit in schools for the sustainable development education as teaching-learning method could achieve extended effect for the earth environmental conservation.

• 국제초고층학회논문집
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• 제1권4호
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• pp.301-310
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• 2012

Buildings, energy and the environment are key issues that the building professions and energy policy makers have to address, especially in the context of sustainable development. With more tall buildings constructed in China, the impact on energy consumption and carbon emission would be great from buildings (2% increase of carbon dioxide annually between 1971 and 2004). The imperative was to investigate the building energy performance of high-rise in different climate zones and identify the key design parameters that impose significantly influence on energy performance in sustainable building design. Design implications on glazing performance, sizing of the ventilation fans, renewable energy application on high-rise building design are addressed. Combination of effective sustainable building design strategies (e.g., building envelope improvement, daylight harvesting, advanced lighting design, displacement ventilation, chilled ceiling etc.) could contribute more than 25% of the total building energy consumption compared to the international building energy code.

• Journal of Microbiology and Biotechnology
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• 제24권11호
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• pp.1551-1558
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• 2014

The esterase gene Est8 from the thermophilic bacterium Bacillus sp. K91 was cloned and expressed in Escherichia coli. The monomeric enzyme exhibited a theoretical molecular mass of 24.5 kDa and an optimal activity around $50^{\circ}C$ at pH 9.0. A model of Est8 was constructed using a hypothetical YxiM precursor structure (2O14_A) from Bacillus subtilis as template. The structure showed an ${\alpha}/{\beta}$-hydrolase fold and indicated the presence of a typical catalytic triad consisting of Ser-11, Asp-182, and His-185, which were investigated by site-directed replacements coupled with kinetic characterization. Asp-182 and His-185 residues were more critical than the Ser-11 residue in the catalytic activity of Est8. A comparison of the amino acid sequence showed that Est8 could be grouped into the GDSL family and further classified as an SGNH hydrolase. Est8 is a new member of the SGNH hydrolase subfamily and may employ a different catalytic mechanism.

• Bulletin of the Korean Chemical Society
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• 제32권4호
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• pp.1368-1370
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• 2011

• Journal of Radiation Protection and Research
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• 제33권4호
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• pp.143-150
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• 2008

In this study, we examined the effect of hydroxyl radical generated by $\gamma$-ray and UV irradiation on shrinkage of vitreous body. Change in gel ratio of vitreous body and change in the properties of its components (collagen, sodium hyaluronate) were analyzed. By comparing these results, the amount of hydroxyl radical, which induces the considerable shrinkage of vitreous body, was evaluated from theoretical calculation based on experimental condition and some reported kinetic parameters. It was concluded that the integrated amount of hydroxyl radical required to liquefy half of the vitreous body (Vitreous body gel ratio = 50%) was estimated as $140\;{\mu}molg^{-1}$ from $\gamma$-ray irradiation experiment. Also, from UV irradiation experiment result, it was confirmed that the effect of hydroxyl radical is larger than that of other reactive species. The causes of shrinkage of vitreous body are supposed as follows, 1) decrease in viscosity by cleavage of glycoside bond in sodium hyaluronate, 2) leaching of collagen from vitreous body and 3) leaching of crosslinked products and scission products of collagen.

• 한국막학회:학술대회논문집
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• 한국막학회 2008년도 춘계 총회 및 학술발표회
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• pp.189-193
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• 2008

• Journal of Microbiology and Biotechnology
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• 제26권4호
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• pp.730-738
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• 2016

est19 is a gene from Bacillus sp. K91 that encodes a new esterase. A comparison of the amino acid sequence showed that Est19 has typical Ser-Gly-Asn-His (SGNH) family motifs and could be grouped into the SGNH hydrolase family. The Est19 protein was functionally cloned, and expressed and purified from Escherichia coli BL21(DE3). The enzyme activity was optimal at 60℃ and pH 9.0, and displayed esterase activity towards esters with short-chain acyl esters (C₂-C₆). A structural model of Est19 was constructed using phospholipase A1 from Streptomyces albidoflavus NA297 as a template. The structure showed an α/β-hydrolase fold and indicated the presence of the typical catalytic triad Ser49-Asp227-His230, which were further investigated by site-directed mutagenesis. To the best of our knowledge, Est19 is a new member of the SGNH hydrolase family identified from thermophiles, which may be applicable in the industrial production of semisynthetic β-lactam antibiotics after modification.