• Journal of Life Science
• /
• v.22 no.10
• /
• pp.1295-1306
• /
• 2012

A microorganism producing carboxymethylcellulase (CMCase) was isolated from seawater and identified as Bacillus atrophaeus. This species was designated as B. atrophaeus LBH-18 based on its evolutionary distance and the phylogenetic tree resulting from 16S rDNA sequencing and the neighbor-joining method. The optimal conditions for rice bran (68.1 g/l), peptone (9.1 g/l), and initial pH (7.0) of the medium for cell growth was determined by Design Expert Software based on the response surface method; conditions for production of CMCase were 55.2 g/l, 6.6 g/l, and 7.1, respectively. The optimal temperature for cell growth and the production of CMCase by B. atrophaeus LBH-18 was $30^{\circ}C$. The optimal conditions of agitation speed and aeration rate for cell growth in a 7-l bioreactor were 324 rpm and 0.9 vvm, respectively, whereas those for production of CMCase were 343 rpm and 0.6 vvm, respectively. The optimal inner pressure for cell growth and production of CMCase in a 100-l bioreactor was 0.06 MPa. Maximal production of CMCase under optimal conditions in a 100-l bioreactor was 127.5 U/ml, which was 1.32 times higher than that without an inner pressure. In this study, rice bran was developed as a carbon source for industrial scale production of CMCase by B. atrophaeus LBH-18. Reduced time for the production of CMCase from 7 to 10 days to 3 days by using a bacterial strain with submerged fermentation also resulted in increased productivity of CMCase and a decrease in its production cost.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.30 no.1
• /
• pp.13-19
• /
• 2024

An analysis of the coastal water temperature in the Tongyeong waters, the eastern sea of the South Sea of Korea, revealed that the water temperature rose sharply before the typhoon made landfall. The water temperature rise occurred throughout the entire water column. An analysis of the sea surface temperature data observed by NOAA(National Oceanic and Atmospheric Administration) satellites, indicated that sea water with a temperature of 30℃ existed in the eastern waters of the eastern South Sea of Korea before the typhoon landed. The southeastern sea of Korea is an area where ocean currents prevail from west to east owing to the Tsushima Warm Current. However, an analysis of the satellite data showed that seawater at 30℃ moved from east to west, indicating that it was affected by the Ekman transport caused by the typhoon before landing. In addition, because the eastern waters of the South Sea are not as deep as those of the East Sea, the water temperature of the entire water layer may remain constant owing to vertical mixing caused by the wind. Because the rise in water temperature in each water layer occurred on the same day, the rise in the bottom water temperature can be considered as owing to vertical mixing. Indeed, the southeastern sea of Korea is a sea area where the water temperature can rise rapidly depending on the direction of approach of the typhoon and the location of high temperature formation.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.16 no.4
• /
• pp.339-344
• /
• 2010

Oxygen depleted water mass can damage aquatic animals not only in direct way but also in indirect way by generating toxic substances including occurrence of hydrogen sulfide and ammonia which are also highly detrimental to animal life in the water mass. An oxygen dissolution device was developed, which makes turnover of the oxygen rich (over 20 mg/L) surface water down to the bottom where hypoxia is evident and tested the device in terms of oxygen recovery in the oxygen depleted bottom water. the device with turnover rates of $3.6\;m^2$/min at the liquid oxygen injection rate of 48~26.3 L/min could recover dissolved oxygen level to 7~25 mg/L at depth 7 m to lead to the dissolution level of over 90% by the supply of liquid oxygen. The running advantage of the device is that it does not require any auxiliary tank and higher energy for operation. Therefore, it can be highly useful device to relieve damages to the farmed animals in the oxygen depleted waters.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.26 no.6
• /
• pp.681-688
• /
• 2020

Approximately 6,000 chemicals are transported through the sea, including hazardous and noxious substances (HNS), which cause marine pollution and are harmful to marine life. The HNS discharged into the sea during the maritime transportation process undergoes physical and chemical changes on the sea surface and in seawater, and some types of HNS sink and are deposited on the seabed. The HNS deposited on the seabed adversely affects the benthic ecosystem, and hence, it is desirable to detect, treat, and recover the HNS on the seabed. Therefore, this study was conducted to analyze the performance requirements that should be considered as the top priority when developing a mechanical system for recovering the HNS deposited on the seabed. Various types of existing dredging devices used for collecting and recovering pollutants from river beds and seabeds were investigated, and 10 performance indices for the mechanical devices were selected. The new performance requirements for the development of the seabed-deposited HNS recovery system were proposed using performance indices. By considering the depth of water in domestic seaports, some of the performance requirements of the mechanical system for recovering deposited HNS from the seabed were obtained as follows: production rate (50-300 ㎥/hr), maximum operation depth (50 m), sediment type (most forms), percentage of solids (10 % or higher), horizontal operating accuracy (±10 cm), limiting currents (3-5 knots). These performance requirements are expected to be useful in the conceptual and basic design of mechanical systems for recovering seabed-deposited HNS.

• Journal of the Korean Society for Marine Environment & Energy
• /
• v.3 no.4
• /
• pp.23-36
• /
• 2000

Bottom sea waters in eight stations including from inner bay to outer bay to understand the importance of the submarine groundwater discharge for the environmental assessment of Chinhae Bay was collected in August 1999 and January 2000. Generally, /sup 222/Rn is very useful tracer to assess the submarine groundwater discharge because it is 2-4 orders of magnitude more concentrated in groundwater compared to surface water. The /sup 222/Rn activities ranged between about 33 to 182 dpm/100kg within the bay. Higher activities more than 100 dpm/100kg were found at the inner bay. The /sup 226/Ra activities, its parent, however, were little different between stations. /sup 222/Rn activities at the same station varied with season. It suggests that the major source of /sup 222/Rn is not from the bottom sediment, but from the change of submarine groundwater discharge by precipitation. The contents of Cl/sup -/ and SO/sub 4//sup 2-/ in the groundwater of Wonjeon-ri were very high as 1,312 and 369 ppm, respectively, indicating that this groundwater along the Chinhae coast was affected by seawater. Therefore, the submarine groundwater in the inner Bay may discharge to the overlying water. It indicates that these submarine groundwater discharges may play an important role as another important source of nutrients in the Chinhae Bay, because groundwater around the Chinhae Bay showed high concentration of dissolved inorganic nutrients (average , nitrate>174 μM, silicate>262 μM). Therefore, further studies are required to assess the contribution by the submarine groundwater discharge in the biogeochemical processes of the Chinhae Bay.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.21 no.8
• /
• pp.579-592
• /
• 2020

This study was designed to assess the water quality and phytoplankton community including chlorophyll a in blood cockle (Tegillarca granosa) farms in May, August and November of 2017 in the northwestern Yeoja Bay, Korea. As a result, the seasonal characteristics of water types by water temperature and salinity were clear. Nutrients were abundant in silicate throughout the season, but phosphate was scarce in spring and summer, and nitrogen sources were scarce in autumn. The species composition of phytoplankton community was a very simple distribution, and the standing crop was also very low. The annual dominant species is dominated by the diatoms, with Skeletonema costatum-ls, Nitzschia longissima in spring, Pleurrosigma normanii, Coscinodiscus gigas in summer, and N. longissima, Pseudonitschia pungens, Chaetoceros curvisetus, Eucampia zodiacus in autumn. In summer the results were different from other coastal waters of Korea. The principal component analysis(PCA) and correlation analysis showed that the characteristics of water quality and biological environments differed according to the season. Furthermore, it was determined by the supply of materials through fresh water on land, seawater congestion caused by the refueling of surface sediments with lower depth, and the balance of biological production and mineralization of organic matters in blood cockle farms.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
• /
• v.4 no.3
• /
• pp.188-197
• /
• 1999

In order to estimate the uranium flux from seawater to sediments, we took pore water samples and deployed benthic chambers on seafloor of Chonsu Bay, Korea. The uranium flux across the sediment-water interface was estimated from the pore water to be 0.112-0.566 mg/$m^2yr$, corresponding to a removal flux of $4.3-21.5{\times}10^7$ gU/yr for the entire Yellow Sea. Nutrient fluxes from sediment to bottom water were estimated to be 135.6 mmol/$m^2yr$ for ammonia, 228.2 mmol/$m^2yr$ for nitrate, 36.8 mmol/$m^2yr$ for phosphate and 23.9 mmol/$m^2yr$ for silicate. The redox boundary, based on the distribution of pore water nitrate and solid phase manganese, was located at 3-5 cm below the sediment surface. Phosphate flux obtained by benthic chambers was 28.S mmol/$m^2yr$. On the other hand, estimates of uranium and silicate fluxes were orders of magnitude greater than those based on pore water profiles. Flux estimates on the basis of pore water concentration is believed to have greater reliability than those obtained from benthic chamber data.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.32 no.2A
• /
• pp.123-130
• /
• 2012

A cooling system is indispensable for the fossil and nuclear power plants which produce electricity by rotating the turbines with hot steam. A cycle of the typical cooling system includes pumping of seawater at the intake pump house, exchange of heat at the condenser, and discharge of hot water to the sea. The cooling type of the nuclear power plants in Korea recently evolves from the conventional surface intake/discharge systems to the submerged intake/discharge systems that minimize effectively an intake temperature rise of the existing plants and that are beneficial to the marine environment by reducing the high temperature region with an intensive dilution due to a high velocity jet and density differential at the mixing zone. It is highly anticipated that the future nuclear power plants in Korea will accommodate the submerged cooling system in credit of supplying the lower temperature water in the summer season. This study investigates the approach flow patterns at the velocity caps and discharge flow patterns from diffusers using the 3-D computational fluid dynamics code of $FLOW-3D^{(R)}$. The approach flow test has been conducted at the velocity caps with and without a cap. The discharge flow from the diffuser was simulated for the single-port diffuser and multi-ports diffuser. The flow characteristics to the velocity cap with a cap demonstrate that fish entrainment can significantly be minimized on account of the low vertical flow component around the cap. The flow pattern around the diffuser is well agreed with the schematic diagram by Jirka and Harleman.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.30 no.5
• /
• pp.882-895
• /
• 1997

Changes in the benthic communities have been studied to investigate the environmental effects before and after the construction of Shihwa Dike in the West coast of Korea. It is suggested that sequential changes in macrofaunal assemblages progressed in two sucressional directions. In the Shihwa lake under the influence of organic enrichment. First, the appearance of 'azoic tone' or 'grossly polluted zone' developed in the area of less than 6 m in depth resulted from the severe dissolved oxygen depletion due to the eutrophication from the increased organic loading. Second, the 'polluted zone' characterized by the proliferation of the opportunistic species in organically enriched area, was found in the vicinity of the industrial discharges and nearby fluvial inputs. This benthic community succession in the Shihwa lake seemed to be caused by the various ecological events such as an eutrophication in this organically enriched environment after construction of the dike and other physico-chemical parameters like salinity and dissolved oxygen in the bottom water, which may be influenced by the irregular surface water discharge and dilution by outer seawater inflow through the water gate of the dike. On the other hand, the benthic communities in the outside of the dike showed that the species richness was more than doubled and the abundance increased almost seven times more than that before the dike construction. This may be a typical characteristics of the initial phase in benthic eutrophication, suggesting that an increased organic input area may have been reponsible for this faunal change in the study area.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
• /
• v.6 no.4
• /
• pp.297-306
• /
• 2008

This study was carried out to identify the characteristics of hydrochemistry controlling groundwater chemical condition in a repository site of Gyeongju. For this study, 12 bore holes of all monitoring bore holes in the study area were selected and total 46 groundwater samples were collected with depth. In addition, 3 surfacewater samples and 1 seawater sample were collected. For water samples, cations and anions were analyzed. The environmental isotopes(${\delta}^{18}O-{\delta}D$, Tritium, ${\delta}^{13}C,\;{\cdot}{\delta}^{34}S$) were also analyzed to trace the origin of water and solutes. The result of ${\delta}^{18}O\;and\;{\delta}D$ analysis showed that surface water and groundwater were originated from precipitation. Tritium concentrations of groundwater decreased with depth but high concentrations of tritium indicated that groundwater was recharged recently. The results of ion and correlation analysis showed that groundwater types of the study area were represented by Ca-Na-$HCO_3$ and Na-Cl-$SO_4$, which was caused by sea spray and water-rock interaction. Especially, high ratio of Na content in groundwater resulted from ion exchange. For redox condition of groundwater, the values of DO and Eh decreased with depth, which indicated that reducing condition was formed in deeper groundwater. In addtion, high concentration of Fe and Mn showed that redox condition of groundwater was controlled by the reduction of Fe and Mn oxides.