• Title/Summary/Keyword: surface sampling

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Atmospheric Bioaerosol, Bacillus sp., at an Altitude of 3,500 m over the Noto Peninsula: Direct Sampling via Aircraft

  • Kobayashi, Fumihisa;Morosawa, Shinji;Maki, Teruya;Kakikawa, Makiko;Yamada, Maromu;Tobo, Yutaka;Hon, Chun-Sang;Matsuki, Atsushi;Iwasaka, Yasunobu
    • Asian Journal of Atmospheric Environment
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    • v.5 no.3
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    • pp.164-171
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    • 2011
  • This work focuses on the analysis of bioaerosols in the atmosphere at higher altitudes over Noto Peninsula, Japan. We carried out direct sampling via aircraft, separated cultures, and identified present isolates. Atmospheric bioaerosols at higher altitudes were collected using a Cessna 404 aircraft for an hour at an altitude of 3,500 m over the Noto Peninsula. The aircraft-based direct sampling system was devised to improve upon the system of balloon-based sampling. In order to examine pre-existing microorganism contamination on the surface of the aircraft body, bioaerosol sampling was carried out just before takeoff using the same method as atmospheric sampling. Identification was carried out by a homology search for 16S or 18S rDNA isolate sequences in DNA databases (GenBank). Isolate sampling just before takeoff revealed Stretpomyces sp., Micrococcus sp., and Cladosporium sp. One additional strain, Bacillus sp., was isolated from the sample after bioaerosol collection at high altitude. As the microorganism contamination on the aircraft body before takeoff differed from that while in the air, the presence of additional, higher atmosphere-based microorganisms was confirmed. It was found that Bacillus sp. was floating at an altitude of 3,500 m over Noto Peninsula.

Serviceability reliability analysis of cable-stayed bridges

  • Cheng, Jin;Xiao, Ru-Cheng
    • Structural Engineering and Mechanics
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    • v.20 no.6
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    • pp.609-630
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    • 2005
  • A reliability analysis method is proposed in this paper through a combination of the advantages of the response surface method (RSM), finite element method (FEM), first order reliability method (FORM) and the importance sampling updating method. The accuracy and efficiency of the method is demonstrated through several numerical examples. Then the method is used to estimate the serviceability reliability of cable-stayed bridges. Effects of geometric nonlinearity, randomness in loading, material, and geometry are considered. The example cable-stayed bridge is the Second Nanjing Bridge with a main span length of 628 m built in China. The results show that the cable sag that is part of the geometric nonlinearities of cable-stayed bridges has a major effect on the reliability of cable-stayed bridge. Finally, the most influential random variables on the reliability of cable-stayed bridges are identified by using a sensitivity analysis.

A Magnet Pole Shape Optimization of a Large Scale BLDC Motor Using a RSM With Design Sensitivity Analysis (민감도기법과 RSM을 이용한 대용량 BLDC 전동기 영구자석의 형상 최적화)

  • Shin, Pan-Seok;Chung, Hyun-Koo;Woo, Sung-Hyun
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.58 no.4
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    • pp.735-741
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    • 2009
  • This paper presents an algorithm for the permanent magnet shape optimization of a large scale BLDC(Brushless DC) motor to minimize the cogging torque. A response surface method (RSM) using multiquadric radial basis function is employed to interpolate the objective function in design parameter space. In order to get a reasonable response surface with relatively small number of sampling data points, additional sampling points are added on the basis of design sensitivity analysis computed by using FEM. The algorithm has 2 stages: the first stage is to determine the PM arc angle, and the 2nd stage is to optimize the magnet pole shape. The developed algorithm is applied to a 5MW BLDC motor to get a minimum cogging torque. After 3 iterations with 4 design parameters, the cogging torque is reduced to 13.2% of the initial one.

Design Optimization of a Staggered Dimpled Channel Using Neural Network Techniques (신경회로망기법을 사용한 엇갈린 딤플 유로의 최적설계)

  • Shin, Dong-Yoon;Kim, Kwang-Yong
    • The KSFM Journal of Fluid Machinery
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    • v.10 no.3 s.42
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    • pp.39-46
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    • 2007
  • This study presents a numerical procedure to optimize the shape of staggered dimple surface to enhance turbulent heat transfer in a rectangular channel. The RBNN method is used as an optimization technique with Reynolds-averaged Navier-Stokes analysis of fluid flow and heat transfer with shear stress transport (SST) turbulence model. The dimple depth-to-dimple print diameter (d/D), channel height-to-dimple print diameter ratio (H/D), and dimple print diameter-to-pitch ratio (D/S) are chosen as design variables. The objective function is defined as a linear combination of heat transfer related term and friction loss related term with a weighting factor. Latin Hypercube Sampling (LHS) is used to determine the training points as a mean of the design of experiment. The optimum shape shows remarkable performance in comparison with a reference shape.

Optimization of Boss Shape for Damage Reduction of the Press-fitted Shaft End (압입축 끝단의 손상저감을 위한 보스부 형상 최적설계)

  • Byon, Sung-Kwang
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.14 no.3
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    • pp.85-91
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    • 2015
  • The press-fit shaft is an important part used in automobiles, vessels, and trains. This study proposes an optimized design method to reduce damage that may occur in the press-fitted shaft by modifying the shape of the boss step of the press-fitted shaft. To reduce the time and cost of running the optimized design method, an approximate design optimization is applied and an optimized algorithm is generated using a genetic algorithm that is widely used in engineering fields and an approximate model using a response surface method. The planned experiments for the data that are needed to generate the approximate model use a central composite design (CCD) and Latin hypercube sampling (LHS), and the results of the approximate optimization using the above two design of experiments are to be compared.

DESIGN OPTIMIZATION OF A STAGGERED DIMPLED CHANNEL TO ENHANCE TURBULENT HEAT TRANSFER (열전달성능 향상을 위한 엇갈린 딤플 유로의 최적설계)

  • Shin, D.Y.;Kim, K.Y.
    • 한국전산유체공학회:학술대회논문집
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    • 2007.04a
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    • pp.159-162
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    • 2007
  • This study presents a numerical procedure to optimize the shape of a staggered dimpled surface to enhance the turbulent heat transfer in a rectangular channel. A optimization technique based on neural network is used with Reynolds-averaged Navier-Stakes analysis of the fluid flow and heat transfer with Shear Stress Transport turbulence model. The dimple depth-to-dimple print diameter ratio, channel height-to-dimple print diameter ratio, and dimple print diameter-to-pitch ratio are chosen as design variables. The objective function is defined as a linear combination of terms related to heat transfer and friction loss with a weighting factor. Latin Hypercube Sampling is used to determine the training points as a mean of the Design of Experiment. Optimal values of the design variables were obtained in a range of the weighting factor.

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Measurement of Dry Deposition Flux of Air Pollutants to the Waterbody (수체로의 대기오염물질 건식침적량 측정)

  • 김영성;진현철;김용표
    • Journal of Korean Society for Atmospheric Environment
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    • v.20 no.3
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    • pp.421-426
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    • 2004
  • Dry deposition fluxes of inorganic acidic species to the waterbody were measured by the dry deposition sampler (DDS). DDS was composed of three pans filled with pure water. An average concentration increase during the sampling time, after removing an abnormal value if existed, was considered as the input by deposition. Important operation parameters such as the amount of water used and sampling time were determined through a series of laboratory experiments. The deposition flux measured by DDS was compared with that by the water surface sampler developed by Yi et ai. (1997a,b).

Use of Graphite Plate for Homogeneous Sample Preparation in Matrix/Surface-assisted Laser Desorption and Ionization of Polypropyleneglycol and Polystyrene

  • Kim, Jeong Hwan;Gang, Wi Gyeong
    • Bulletin of the Korean Chemical Society
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    • v.21 no.4
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    • pp.401-404
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    • 2000
  • Matrix/Surface-assisted laser desorption/ionization (M/SALDI) mass spectrometry of polypropylene glycol and polystyrene, directly deposited on graphite plate, is demonstrated. Graphite plate is effective both as an en-ergy transfer medium and robu st sampling support for LDI of polymers. Mass spectra ofpolymers can be easily obtained due to homogeneous distribution on graphite surface and their ion signals are long-lived by large ef-fective desorption volume enough to investigate M/SALDI process.