• BMB Reports
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• v.31 no.3
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• pp.240-244
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• 1998

Helicobacter pylori is the etiologic agent of human gastritis and peptic ulceration and produces urease as the major protein component on its surface. H. pylori urease is known to serve as a major virulence factor and in a potent immunogen. In order to express the recombinant urease at a higher level, a DNA fragment containing the minimal H. pylori urease gene cluster was subcloned into a high copy-number vector. The recombinant H. pylori urease expressed in an E. coli strain that was grown in a rich medium supplemented with added nickel was purified to near homogeneity by using DEAE-Sepharose, Superdex HR200, and Mono-Q (FPLC) columns and the purified enzyme possessed the specific activity of 1255 U/mg. Polyclonal antibodies raised against the purified recombinant H. pylori urease were shown to be very specific when subjected to Western blot analysis, in which crude extracts from the H. pylori ATCC strain and the recombinant E. coli strains expressing various bacterial ureases were exnmined for cross-reactivity.

• Tribology and Lubricants
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• v.11 no.2
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• pp.34-43
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• 1995

The boundary films formed in sliding on steel surfaces were characterized using various lubricants. The mechanism of boundary film formation and loss was investigated over a range of temperature. The thickness of the boundary films was monitored in-situ by an ellipsometer, and the composition of the films was analyzed by XPS. The performance of the lubricants is closely associated with boundary film forming ability. In order to achieve high load carrying capacity, a boundary film must be formed on the surface. Sliding is necessary to form the films and some time is also required. As temperature increases, chemical reactivity increases the film formation rate, while the film removal rate increases due to thg decrease of durability of the boundary film material. There is a balance between these two competing mechanisms and this balance is reflected in the boundary film thickness.

• Journal of the Korean Applied Science and Technology
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• v.27 no.1
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• pp.87-92
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• 2010

Silicone surfactants are widely used in many industrial area because of its thermal stability and lower foaming property. But it has limitation to expand the application because of migration and bubble generation issues when it is mixed with organic surfactant. In this study, epoxy functionalized fluoro-silicone surfactant, perfluoro glycidoxypropyl polyether siloxane(PFGES), was synthesized using hydrosilylation reaction among perfluoro methyl hydrogen siloxane, allyl glycidyl ether, and allyl ployether in order to get lower surface tension, better thermal stability than conventional silicone surfactant, and reactivity with anhydride function.

• Carbon letters
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• v.10 no.2
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• pp.123-130
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• 2009

Multi-elements doped $TiO_2$ was prepared as a new photocatalyst in order to decrease the band gap of $TiO_2$ by sol-gel process which can provide the large active sites of $TiO_2$. Multi-elements were doped by using a single precursor, tetraethylammonium tetrafluoroborate (TEATFB). By the benefit of large specific surface area of $TiO_2$ prepared by sol-gel process, catalysts showed initial fast removal of dye. The photoactivity showed that the doped catalysts significantly promote the light reactivity than undoped $TiO_2$. The commendable photoactivity of prepared catalysts is predominantly attributable to the doping of anions which may reduce the band gap.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.10 no.1
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• pp.30-35
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• 2000

A floating solidification technique is a process applied extensively to the production of glass plates. We used the technique for fabrication of Si plate. We used the proper liquid substrate material of 50 wt% $SiO_2$- 21 wt% $A1_2$$O_3$-29 wt% MnO, after studying ternary phase diagram of various oxides systems. The liquid substrate material has proper properties in the aspects of temperature, density and reactivity. But results showed some problems in fluidity and surface tension of the liquid substrate material.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.24 no.4
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• pp.280-284
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• 2011

Silicon carbide (SiC)-based gas sensors can be operated at very high temperatures. So far, catalytic metal-schottky diodes respond fast to a change between a reducing and an oxidizing atmosphere. Therefore SiC diodes have been suggested for high temperature gas sensor applications. In this work, the effect of reactivity of the catalytic surface on the 4H-SiC sensor-structures in 375 K~775 K have been studied and some fundamental simulations have also been performed.

• Journal of the Korean Society of Safety
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• v.23 no.6
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• pp.1-6
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• 2008

Machine operator and quality are affected by chip during cutting process to product machine parts. This paper presents a study of the influence of cutting conditions on the surface roughness obtained by turning using Taguchi method for safety of turning operator. In the machining of titanium alloy, high cutting temperature and strong chemical affinity between the tool and the work material are generated because of its low thermal conductivity and chemical reactivity. Therefore titanium alloys are known as difficult-to materials. An orthogonal array, the signal-to-noise ratio, the analysis of variance are employed to investigate the cutting characteristics of implant material bars using tungsten carbide cutting tools of throwaway type. Also Experimental results by orthogonal array are compared with optimal condition to evaluate advanced reliability. Required simulations and experiments are performed, and the results are investigated.

• Bulletin of the Korean Chemical Society
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• v.19 no.7
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• pp.754-760
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• 1998

It was found that the adsorption of a cobalt(III) complex with a macrocyclic ligand, C-meso-5,7,7,12,14,14-hexamethyl-1,4,8,11-tetraazacyclotetradecane (hmc), was induced on a glassy carbon electrode by heavily oxidizing the electrode surface. Adsorption properties are discussed. The glassy carbon electrode with the adsorbed complex was employed to see the catalytic activities for the electro-reduction of O2. In the presence of oxygen, reduction of (hmc)Co3+ showed two cathodic waves in cyclic voltammetry. Compared to the edge plane graphite electrode at which two cathodic waves were also observed in a previous study, catalytic reduction of O2 occurred in the potential region of the first wave while it happened in the second wave region with the other electrode. A rotating disk electrode after the same treatment was employed to study the mechanism of the O2 reduction and two-electron reduction of O2 was observed. The difference from the previous results was explained by the different reactivity of the (hmc)CoOOH2+ intermediate, which is produced after the two electron reduction of (hmc)Co3+ in the presence of O2.

• Nuclear Engineering and Technology
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• v.56 no.3
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• pp.772-784
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• 2024

The hexagonal nodal code RENUS has been enhanced to handle irregularly deformed hexagonal assemblies. The underlying RENUS methods involving triangle-based polynomial expansion nodal (T-PEN) and corner point balance (CPB) were extended in a way to use line and surface integrals of polynomials in a deformed hexagonal geometry. The nodal calculation is accelerated by the coarse mesh finite difference (CMFD) formulation extended to unstructured geometry. The accuracy of the unstructured nodal solution was evaluated for a group of 2D SFR core problems in which the assembly corner points are arbitrarily displaced. The RENUS results for the change in nuclear characteristics resulting from fuel deformation were compared with those of the reference McCARD Monte Carlo code. It turned out that the two solutions agree within 18 pcm in reactivity change and 0.46% in assembly power distribution change. These results demonstrate that the proposed unstructured nodal method can accurately model heterogeneous thermal expansion in hexagonal fueled cores.

• Bulletin of the Korean Chemical Society
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• v.33 no.5
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• pp.1457-1464
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• 2012

An ecofriendly catalytic route for selective synthesis of $N$-substituted azacyclopentanes, nitrogen-containing heterocyclic intermediates for many bioactive compounds, was established by carrying out $N$-heterocyclization (di $N$-alkylation) of primary amines with 1,4-dichloro butane (as dialkylating agent) using catalytic amount of hydrotalcite as solid base catalyst. The hydrotalcite was found to be efficient solid base catalyst for di $N$-alkylation of different primary amines (aniline, benzyl amine, cyclohexyl amine and n-butyl amine) giving 82 to 96% conversion (at optimized reaction condition) of 1,4-dichloro butane and > 99% selectivity of respective $N$-substituted azacyclopentanes within 30 min. under solvent free condition. The reaction parameters significantly influence the conversion of 1,4-dichloro butane to $N$-substituted azacyclopentanes. The nature of substituent present on amino group affects the reactivity of amine substrates for di $N$-alkylation reaction with 1,4-dichloro butane. The 1,4-dichloro butane was found to be highly reactive alkylating agent for di $N$-alkylation of amines as compared to 1,4-dihydroxy butane. The reusability of the catalyst and its chemical stability in the reaction was demonstrated.